<p><strong>All the participants remembered our dear colleagues and friends who left us in the past years with a minute of silence. After the introductory briefing for the participants, the special guest of the conference Prof. Ozaki obtained the Ioannes Marcus Marci medal for his extraordinary achievements in the field of molecular spectroscopy.</strong></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Minuta_ticha_za_nase_drahe_kolegy_a_kamarady_kteri_nas_v_minulych_letech_opustili_4710c59577/SSJMM-Minuta-ticha-za-nase-drahe-kolegy-a-kamarady-kteri-nas-v-minulych-letech-opustili.webp" alt="SSJMM: Minuta ticha za naše drahé kolegy a kamarády, kteří nás v minulých letech opustili"/></p>
<p><strong>Prof. Ozaki</strong> started the sequence of lectures with a very interesting topic entitled <strong>&quot;Chiral-label-free SERS and TERS strategies for chiral discrimination&quot;</strong>, which was followed by other very engaging lectures from both the <strong>molecular</strong> and <strong>atomic</strong> sections until the afternoon hours. Breaks were used for refreshments and visits of sponsor stands.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Oceneni_Prof_Ozakiho_medaili_Jana_Marka_Marci_23c43d0109/SSJMM-Oceneni-Prof.-Ozakiho-medaili-Jana-Marka-Marci.webp" alt="SSJMM: Ocenění Prof. Ozakiho medailí Jana Marka Marci"/></p>
<p>The afternoon poster session brought passionate <strong>scientific discussions</strong> between the participants and new acquaintances were made here. Wine, beer, coffee and snacks in the form of buffets were also served here. Thanks to the beautiful weather, participants were able to enjoy drinks and snacks on the <strong>roof terrace</strong>.</p>
<p>In the evening, a small surprise was prepared for the conference participants in the form of <strong>a trip to the observatory on a special conference tram</strong> (<strong>the &quot;Šalina&quot;</strong>). After a short hike to the observatory on Kraví hora, a scientific program was prepared in the <strong>3D digital room</strong>, where the participants learned interesting <strong>facts about stars</strong> and <strong>constellations</strong> and then enjoyed 2 films called <strong>&quot;Voyager&quot;</strong> and <strong>&quot;We came from the stars&quot;</strong>.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Konferencni_tramvaj_ESAS_CSSC_2022_salinou_b08bb9db74/SSJMM-Konferencni-tramvaj-ESAS-CSSC-2022-(salinou%22)..webp" alt="SSJMM: Konferenční tramvaj ESAS-CSSC 2022 („šalinou“)"/></p>
<p>For those interested, in the middle of the night the astronomical observatory offered the opportunity to <strong>see the moon</strong>, <strong>the planets</strong> of <strong>the solar system</strong>, stars and nebulae underlined by scientific talk.</p>
<p><strong>To be continued on Tuesday….</strong></p>
<blockquote>
<p>💡💡💡 <strong><a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ESAS_2022_BOOK_OF_ABSTRACTS_a222376ad0/ESAS-2022-BOOK-OF-ABSTRACTS.pdf">BOOK OF ABSTRACTS ESAS-CSSC 2022</a></strong></p>
</blockquote>
<h3><strong>Programme monday 5th of september 2022</strong></h3>
<h5><strong>8:00-8:30 OPENING CEREMONY</strong></h5>
<ul>
<li>(Hall P31)</li>
<li>KANICKÝ, MATĚJKA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_OPENING_CEREMONY_KANICKY_MATEJKA_8a78101e80/SSJMM-OPENING-CEREMONY-(KANICKY-MATEJKA).webp" alt="SSJMM: OPENING CEREMONY (KANICKÝ, MATĚJKA)"/></p>
<h4><strong>SURFACE-ENHANCED RAMAN SPECTROSCOPY</strong></h4>
<ul>
<li>(Hall P31) CHAIR: MATĚJKA</li>
</ul>
<h5><strong>8:30 –9:15 Chiral-label-free SERS and TERS strategies for chiral discrimination</strong></h5>
<ul>
<li>PL1: Y. OZAKI</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Chiral_label_free_SERS_and_TERS_strategies_for_chiral_discrimination_Y_OZAKI_a336b3d8a0/SSJMM-Chiral-label-free-SERS-and-TERS-strategies-for-chiral-discrimination-(Y.-OZAKI).webp" alt="SSJMM: Chiral-label-free SERS and TERS strategies for chiral discrimination (Y. OZAKI)"/></p>
<h5><strong>9:15 –9:35 SERS and SERRS spectroscopy as a tool for probing and monitoring plasmon catalysed decarboxylation of Ru(II)-dicarboxybipyridine complexes</strong></h5>
<ul>
<li>OL 1: B. VLČKOVÁ</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_SERS_and_SERRS_spectroscopy_as_a_tool_for_probing_and_monitoring_plasmon_catalysed_decarboxylation_of_Ru_II_dicarboxybipyridine_complexes_B_VLCKOVA_092cfb1344/SSJMM-SERS-and-SERRS-spectroscopy-as-a-tool-for-probing-and-monitoring-plasmon-catalysed-decarboxylation-of-Ru(II)-dicarboxybipyridine-complexes-(B.-VLCKOVA).webp" alt="SSJMM: SERS and SERRS spectroscopy as a tool for probing and monitoring plasmon catalysed decarboxylation of Ru(II)-dicarboxybipyridine complexes (B. VLČKOVÁ)"/></p>
<h5><strong>9:35 –9:55 New approaches in plasma-assisted nanostructured platforms for surface-enhanced Raman scattering (SERS) spectroscopy</strong></h5>
<ul>
<li>OL 2: M. PROCHÁZKA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_New_approaches_in_plasma_assisted_nanostructured_platforms_for_surface_enhanced_Raman_scattering_SERS_spectroscopy_M_PROCHAZKA_47a1aad288/SSJMM-New-approaches-in-plasma-assisted-nanostructured-platforms-for-surface-enhanced-Raman-scattering-(SERS)-spectroscopy-(M.-PROCHAZKA).webp" alt="SSJMM: New approaches in plasma-assisted nanostructured platforms for surface-enhanced Raman scattering (SERS) spectroscopy (M. PROCHÁZKA)"/></p>
<h5><strong>9:55 –10:15 Molecularspectroscopy NICOLET 1992-2022</strong></h5>
<ul>
<li>OL 3: J. PÁSZTOR</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Molecularspectroscopy_NICOLET_1992_2022_J_PASZTOR_e28b7f046a/SSJMM-Molecularspectroscopy-NICOLET-1992-2022-(-J.-PASZTOR).webp" alt="SSJMM: Molecularspectroscopy NICOLET 1992-2022 (J. PÁSZTOR)"/></p>
<h5><strong>10:15 –10:35 The integration of a handheld Raman device and a SERS-based sensor forspectral recognition of vaginal fluids alterations</strong></h5>
<ul>
<li>OL 4: S. BERUS</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_The_integration_of_a_handheld_Raman_device_and_a_SERS_based_sensor_forspectral_recognition_of_vaginal_fluids_alterations_S_BERUS_6ed701fc1d/SSJMM-The-integration-of-a-handheld-Raman-device-and-a-SERS-based-sensor-forspectral-recognition-of-vaginal-fluids-alterations-(S.-BERUS).webp" alt="SSJMM: The integration of a handheld Raman device and a SERS-based sensor forspectral recognition of vaginal fluids alterations (S. BERUS)"/></p>
<h5><strong>10:35 –11:05 COFFEE BREAK</strong></h5>
<ul>
<li>ATRIUMFACULTY OF SOCIAL STUDIES</li>
</ul>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>RAMAN SPECTROSCOPY &amp; IR SPECTROSCOPY 1</strong></h4>
<ul>
<li>(Hall P31) CHAIR: JOHNSON</li>
</ul>
<h5><strong>11:05 – 11:35 Drop coating deposition Raman (DCDR) spectroscopy for sensitive detection of biologically important molecules</strong></h5>
<ul>
<li>IL 1: E. KOČIŠOVÁ</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Drop_coating_deposition_Raman_DCDR_spectroscopy_for_sensitive_detection_of_biologically_important_molecules_E_KOCISOVA_b5e3d3643e/SSJMM-Drop-coating-deposition-Raman-(DCDR)-spectroscopy-for-sensitive-detection-of-biologically-important-molecules-(E.-KOCISOVA).webp" alt="SSJMM: Drop coating deposition Raman (DCDR) spectroscopy for sensitive detection of biologically important molecules (E. KOČIŠOVÁ)"/></p>
<h5><strong>11:35 – 12:05 Structure and interactions of amyloid-β peptide aggregates unraveled by novel infrared spectroscopy approaches</strong></h5>
<ul>
<li>IL 2: A. BARTH</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Structure_and_interactions_of_amyloid_v_peptide_aggregates_unraveled_by_novel_infrared_spectroscopy_approaches_A_BARTH_66298556f7/SSJMM-Structure-and-interactions-of-amyloid-b-peptide-aggregates-unraveled-by-novel-infrared-spectroscopy-approaches-(A.-BARTH).webp" alt="SSJMM: Structure and interactions of amyloid-β peptide aggregates unraveled by novel infrared spectroscopy approaches (A. BARTH)"/></p>
<h5><strong>12:05 – 12:25 Raman microscopy survey of intracellular purine inclusions: New paradigm of biocrystallization in eukaryotes</strong></h5>
<ul>
<li>OL 5: P. MOJZEŠ</li>
</ul>
<h5><strong>12:25 – 12:45 Raman optical activity as a promising tool for mononucleotide g-quadruplexes studies</strong></h5>
<ul>
<li>OL 6: V. PROFANT</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Raman_optical_activity_as_a_promising_tool_for_mononucleotide_g_quadruplexes_studies_V_PROFANT_62e00f1fd2/SSJMM-Raman-optical-activity-as-a-promising-tool-for-mononucleotide-g-quadruplexes-studies-(V.-PROFANT).webp" alt="SSJMM: Raman optical activity as a promising tool for mononucleotide g-quadruplexes studies (V. PROFANT)"/></p>
<h5><strong>12:45 – 13:05 Fluorescence-free material identification with new XTR technology from Metrohm</strong></h5>
<ul>
<li>OL 7: M. LIBÁNSKÝ</li>
</ul>
<h5><strong>13:05 – 14:45 LUNCH</strong></h5>
<ul>
<li>MU RECTORATE</li>
</ul>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>ICP-MS/OES</strong></h4>
<ul>
<li>(Hall P51) CHAIR: MATOUŠEK</li>
</ul>
<h5><strong>11:05 – 11:35 In-vivo and in-vitro studies on metal release (ions and nanoparticles) from dental implants using single-cell and single-particle ICP-MS</strong></h5>
<ul>
<li>IL 3: M. CORTE-RODRIGUEZ</li>
</ul>
<h5><strong>11:35 – 11:55 Analysis of PTFE micro-particles by ICP-QMS operated in a single-particle mode</strong></h5>
<ul>
<li>OL 8: F. GELMAN</li>
</ul>
<h5><strong>11:55 – 12:15 iCAP TQe ICP-MS: For samples where single quadrupole performance isn&#39;t enough</strong></h5>
<ul>
<li>OL 9: M. GODULA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_i_CAP_T_Qe_ICP_MS_For_samples_where_single_quadrupole_performance_isn_t_enough_M_GODULA_8de9c07538/SSJMM-iCAP-TQe-ICP-MS-For-samples-where-single-quadrupole-performance-isn't-enough-(M.-GODULA).webp" alt="SSJMM: iCAP TQe ICP-MS: For samples where single quadrupole performance isn&#39;t enough (M. GODULA)"/></p>
<h5><strong>12:15 – 12:35 Isotopic tools for uranium ores dating: application of uranium-lead and lead-lead dating systems</strong></h5>
<ul>
<li>OL 10: J. DOBEŠ</li>
</ul>
<h5><strong>12:35 – 12:55 Photochemical vapor generation of rhodium and rhenium - on the way towards real sample analyses</strong></h5>
<ul>
<li>OL 11: J. VYHNANOVSKÝ</li>
</ul>
<h5><strong>13:05 – 14:45 LUNCH</strong></h5>
<ul>
<li>MU RECTORATE</li>
</ul>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>RAMAN SPECTROSCOPY &amp; IR SPECTROSCOPY 2</strong></h4>
<ul>
<li>(Hall P31) CHAIR: BARTH</li>
</ul>
<h5><strong>14:45 – 15:15 Identification of microplastics by micro-FTIR: Validation and uncertainty evaluation</strong></h5>
<ul>
<li>IL 4: R. BETTENCOURT DA SILVA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Identification_of_microplastics_by_micro_FTIR_Validation_and_uncertainty_evaluation_R_BETTENCOURT_DA_SILVA_80796798af/SSJMM-Identification-of-microplastics-by-micro-FTIR-Validation-and-uncertainty-evaluation-(R.-BETTENCOURT-DA-SILVA).webp" alt="SSJMM: Identification of microplastics by micro-FTIR: Validation and uncertainty evaluation (R. BETTENCOURT DA SILVA)"/></p>
<h5><strong>15:15 – 15:35 Microplastics analysis by agilent 8700 LDIR</strong></h5>
<ul>
<li>OL 12: M. HÁKOVÁ</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Microplastics_analysis_by_agilent_8700_LDIR_M_HAKOVA_0de423b84a/SSJMM-Microplastics-analysis-by-agilent-8700-LDIR-(M.-HAKOVA).webp" alt="SSJMM: Microplastics analysis by agilent 8700 LDIR (M. HÁKOVÁ)"/></p>
<h5><strong>15:35 – 15:55 Spectroscopic characterization of humic acids in chernozems</strong></h5>
<ul>
<li>OL 13: K. BOTUROVÁ</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Spectroscopic_characterization_of_humic_acids_in_chernozems_K_BOTUROVA_524c436747/SSJMM-Spectroscopic-characterization-of-humic-acids-in-chernozems-(K.-BOTUROVA).webp" alt="SSJMM: Spectroscopic characterization of humic acids in chernozems (K. BOTUROVÁ)"/></p>
<h5><strong>15:55 – 16:15 Identification of gemstones in watch by Raman spectroscopy</strong></h5>
<ul>
<li>OL 14: J. ŠTUBŇA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Identification_of_gemstones_in_watch_by_Raman_spectroscopy_J_STUBNA_f09efee8c5/SSJMM-Identification-of-gemstones-in-watch-by-Raman-spectroscopy-(J.-STUBNA).webp" alt="SSJMM: Identification of gemstones in watch by Raman spectroscopy (J. ŠTUBŇA)"/></p>
<h5><strong>16:15 – 18:15 POSTER SESSION</strong></h5>
<ul>
<li>AUDITORIUM OF FACULTY OF SOCIAL STUDIES</li>
<li><a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/List_of_Posters_ESAS_CSSC_2022_final_044f000ec5/List_of_Posters_ESAS_CSSC_2022_final.pdf"><strong>List of Posters ESAS-CSSC 2022</strong></a></li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_POSTER_SESSION_ef8cfa3bf9/SSJMM-POSTER-SESSION.webp" alt="SSJMM:  POSTER SESSION"/></p>
<h5><strong>19:30 – 22:00 BRNO OBSERVATORY</strong></h5>
<ul>
<li>SEE DETAILS IN <a href="http://esas-cssc2022.spektroskopie.cz/social_programme">SOCIAL PROGRAMME</a></li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_BRNO_OBSERVATORY_23237e036c/SSJMM-BRNO-OBSERVATORY.webp" alt="SSJMM: BRNO OBSERVATORY"/></p>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>SPECIATION ANALYSIS 1</strong></h4>
<ul>
<li>(Hall P51) CHAIR: FELDMANN</li>
</ul>
<h5><strong>14:45 – 15:15 Instrumental Analysis in 30 minutes: bridging the techniques</strong></h5>
<ul>
<li>IL 5: G. SCHLEMMER</li>
</ul>
<h5><strong>11:15 – 15:35 Simultaneous ultratrace speciation analysis of arsenic and germanium in natural water</strong></h5>
<ul>
<li>OL 15: T. MATOUŠEK</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Simultaneous_ultratrace_speciation_analysis_of_arsenic_and_germanium_in_natural_water_T_MATOUSEK_c5786a1c57/SSJMM-Simultaneous-ultratrace-speciation-analysis-of-arsenic-and-germanium-in-natural-water-(T.-MATOUSEK).webp" alt="SSJMM: Simultaneous ultratrace speciation analysis of arsenic and germanium in natural water (T. MATOUŠEK)"/></p>
<h5><strong>15:35 – 15:55 Speciation analysis of Gd-based contrast agents in river water using HPLC-ICP-MS</strong></h5>
<ul>
<li>OL 16: K. CHIBA</li>
</ul>
<h5><strong>15:55 – 16:15 Miniaturised liquid phase extraction of butyl and phenyltins in sediment samples using deep eutectic solvent: Optimisation and HPLC-ICP-MS analysis</strong></h5>
<ul>
<li>OL 17: P. NOMNGONGO</li>
</ul>
<h5><strong>16:15 – 18:15 POSTER SESSION</strong></h5>
<ul>
<li>AUDITORIUM OF FACULTY OF SOCIAL STUDIES</li>
<li><a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/List_of_Posters_ESAS_CSSC_2022_final_044f000ec5/List_of_Posters_ESAS_CSSC_2022_final.pdf"><strong>List of Posters ESAS-CSSC 2022</strong></a></li>
</ul>
<h5><strong>19:30 – 22:00 BRNO OBSERVATORY</strong></h5>
<ul>
<li>SEE DETAILS IN <a href="http://esas-cssc2022.spektroskopie.cz/social_programme">SOCIAL PROGRAMME</a></li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_BRNO_OBSERVATORY_2_54406cbf64/SSJMM-BRNO-OBSERVATORY-2.webp" alt="SSJMM: BRNO OBSERVATORY"/></p>


ESAS-CSSC 2022 (Day 1)

<p><strong>Prof. Flores</strong> was recognized for his outstanding achievements in the field of <strong>sample preparation for analytical spectrometry</strong>, especially <strong>microwave</strong> and <strong>ultrasound applications</strong>.</p>
<p>The rest of the lectures were then devoted to a lot of interesting topics from the <strong>AAS</strong>, <strong>Raman</strong>, <strong>IR</strong>, <strong>Speciation analysis</strong>, <strong>Luminescence spectroscopy</strong>, <strong>Mass Spectrometry</strong> and <strong>Microwave plasma</strong>.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Predani_medaile_IMM_kterou_v_roce_2022_obdrzel_prof_Erico_Marlon_de_Moraes_Flores_ze_Statni_univerzity_v_Brazilske_Santa_Marii_1cd15ba45b/SSJMM-Predani-medaile-IMM-kterou-v-roce-2022-obdrzel-prof.-Erico-Marlon-de-Moraes-Flores-ze-Statni-univerzity-v-Brazilske-Santa-Marii.webp" alt="SSJMM: The medallist in 2022: prof. Érico Marlon de Moraes Flores from Departamento de Química, Universidade Federal de Santa Maria, Brasil"/></p>
<p>After the lectures, the last <strong>poster session</strong> took place, where small snacks and refreshments were served and there was a great mood.</p>
<p>To be continued on Wednesday….</p>
<blockquote>
<p>💡💡💡 <strong><a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ESAS_2022_BOOK_OF_ABSTRACTS_a222376ad0/ESAS-2022-BOOK-OF-ABSTRACTS.pdf">BOOK OF ABSTRACTS ESAS-CSSC 2022</a></strong></p>
</blockquote>
<h3><strong>Programme tuesday september 6th</strong></h3>
<h4><strong>AAS 1</strong></h4>
<ul>
<li>(Hall P31) CHAIR: DĚDINA</li>
</ul>
<h5><strong>8:30 – 9:20 Advances and trends in sample preparation for analytical spectrometry</strong></h5>
<ul>
<li>PL 2: E. FLORES</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Advances_and_trends_in_sample_preparation_for_analytical_spectrometry_E_FLORES_461494a6a9/SSJMM-Advances-and-trends-in-sample-preparation-for-analytical-spectrometry-(E.-FLORES).webp" alt="SSJMM: Advances and trends in sample preparation for analytical spectrometry (E. FLORES)"/></p>
<blockquote>
<h4><strong>Érico Marlon de Moraes Flores (Departamento de Química, Universidade Federal de Santa Maria, Brazil)</strong></h4>
</blockquote>
<blockquote>
<p>Érico M. M. Flores, born in 1966, has a permanent position at Federal University of Santa Maria (since 1991) in Brazil, performing research and teaching activities as a Full Professor.</p>
</blockquote>
<blockquote>
<p>His research interests include: development of methods for sample preparation, ultrasound and microwave applications, speciation analysis and quality control of environmental samples, pharmaceutical and other industrial products using atomic spectrometry (ICP-MS, ICP-OES, AAS) and ion chromatography. He has been coordinator of more than 60 research projects since 2000, including international cooperation with public and private companies, Brazilian government, etc. More than 8 million USD (total).</p>
</blockquote>
<blockquote>
<p>He has presented more than 50 invited lectures in international meetings (China, USA, Denmark, Germany, Austria, France, Greece, Italy, Czech Republic, Israel, Equator, Argentina, Venezuela, Mexico, Malaysia, Uruguay, Slovakia, Egypt, Turkey, among others) and published more than 380 peer reviewed international papers (including Analytical Chemistry, JAAS, Spectrochimica Acta Part B, Talanta, Analytica Chimica Acta, etc), and many international books and book chapters (over 8000 citations and H index = 45). He authored many patents and developed a new system of microwave induced combustion that is currently commercialized by Anto Paar company (Graz, Austria) in more than 50 countries. More than 33 students have received the PhD and more than 54 have received the MS degree under his supervision.</p>
</blockquote>
<blockquote>
<p>He was member of Editorial Board of Journal of Analytical Atomic Spectrometry/RSC (2014-2019). Currently, he is member of Editorial Board of Atomic Spectroscopy and Editor for Reviews of Brazilian Journal of Analytical Chemistry/BrJAC-Brazil and Editor of Drug Analytical Research journal/Brazil.</p>
</blockquote>
<blockquote>
<p>He received more than 30 national and international awards since 2002. He is a reviewer for more than 50 international scientific journals. He was a chairman or Organizing Committee member of several international (including CSI) and many national meetings. He is Fellow of Royal Society of Chemistry, Associate Member of Analytical Chemistry Division of IUPAC and Deputy Topic Leader of Expert Working Group/EWG-Q3D (Elemental Impurities) of International Conference of Harmonization. He has been a representative member of many national and international committees, namely in 2014-2018 he performed as Scientific Director of Foundation of Rio Grande do Sul State for Research Support.</p>
</blockquote>
<blockquote>
<p>Since 2018, he is the Coordinator of the International Affairs Office of Federal University of Santa Maria and responsible by the international cooperations with foreign nations involving scientific, technological, and academic cooperation as, e.g., with researchers of the Czech Republic among others.</p>
</blockquote>
<h5><strong>9:20 – 9:50 High resolution continuum source AAS for determination of nonmetals</strong></h5>
<ul>
<li>IL 6: E. BEINROHR</li>
</ul>
<h5><strong>9:50 – 10:10 Study of distribution of zinc in ZnO nanoparticles in soil colloids (extracted from samples collected at various agricultural and forest sites in Slovakia) by sequential centrifugation and flame AAS</strong></h5>
<ul>
<li>OL 18: V. CYPRICHOVÁ</li>
</ul>
<h5><strong>10:10 – 10:40 What metals/speciome can tell us about bioanalytics applied to health and plant science</strong></h5>
<ul>
<li>IL 7: M. ARRUDA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_What_metals_speciome_can_tell_us_about_bioanalytics_applied_to_health_and_plant_science_M_ARRUDA_94faf1c193/SSJMM-What-metals-speciome-can-tell-us-about-bioanalytics-applied-to-health-and-plant-science-(M.-ARRUDA).webp" alt="SSJMM: What metals-speciome can tell us about bioanalytics applied to health and plant science (M. ARRUDA)"/></p>
<h5><strong>10:40 – 11:10 COFFEE BREAK</strong></h5>
<ul>
<li>ATRIUM OF FACULTY OF SOCIAL STUDIES</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_COFFEE_BREAK_ATRIUM_OF_FACULTY_OF_SOCIAL_STUDIES_59dc44581b/SSJMM-COFFEE-BREAK-ATRIUM-OF-FACULTY-OF-SOCIAL-STUDIES.webp" alt="SSJMM: COFFEE BREAK - ATRIUM OF FACULTY OF SOCIAL STUDIES"/></p>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>AAS 2</strong></h4>
<ul>
<li>(Hall P31) CHAIR: FLORES</li>
</ul>
<h5><strong>11:10 – 11:40 Plasma induced chemical vapor generation: a new green and efficient sample introduction method for atomic spectroscopy</strong></h5>
<ul>
<li>IL 8: Z. ZHU</li>
</ul>
<h5><strong>11:40 – 12:10 Employing laser induced fluorescence and selected ion flow tube mass spectrometry to understand what happens in hydride atomizers</strong></h5>
<ul>
<li>IL 9: J. DĚDINA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Employing_laser_induced_fluorescence_and_selected_ion_flow_tube_mass_spectrometry_to_understand_what_happens_in_hydride_atomizers_J_DEDINA_a707e11383/SSJMM-Employing-laser-induced-fluorescence-and-selected-ion-flow-tube-mass-spectrometry-to-understand-what-happens-in-hydride-atomizers-(J.-DEDINA).webp" alt="SSJMM: Employing laser induced fluorescence and selected ion flow tube mass spectrometry to understand what happens in hydride atomizers (J. DĚDINA)"/></p>
<h5><strong>12:10 – 12:30 An improved method for the determination of PFAS using HR-CS-GFMAS via GaF detection</strong></h5>
<ul>
<li>OL 19: F. SIMON</li>
</ul>
<h5><strong>12:30 – 12:50 Spectral aspects in determination of fluorine via GaF, CaF and SrF molecules using high-resolution continuum source molecular absorption spectrometry</strong></h5>
<ul>
<li>OL 20: Z. KOWALEWSKA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Spectral_aspects_in_determination_of_fluorine_via_Ga_F_Ca_F_and_Sr_F_molecules_using_high_resolution_continuum_source_molecular_absorption_spectrometry_Z_KOWALEWSKA_67ed8da1a3/SSJMM-Spectral-aspects-in-determination-of-fluorine-via-GaF-CaF-and-SrF-molecules-using-high-resolution-continuum-source-molecular-absorption-spectrometry-(Z.-KOWALEWSKA).webp" alt="SSJMM: Spectral aspects in determination of fluorine via GaF, CaF and SrF molecules using high-resolution continuum source molecular absorption spectrometry (Z. KOWALEWSKA)"/></p>
<h5><strong>12:50 – 14:30 LUNCH</strong></h5>
<ul>
<li>MU RECTORATE</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_LUNCH_MU_RECTORATE_7f66541c4d/SSJMM-LUNCH-MU-RECTORATE.webp" alt="SSJMM: LUNCH - MU RECTORATE"/></p>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>RAMAN SPECTROSCOPY &amp; IR SPECTROSCOPY 3</strong></h4>
<ul>
<li>(Hall P51) CHAIR: KOČIŠOVÁ</li>
</ul>
<h5><strong>11:10 – 11:40 Promising molecular-based materials for nonlinear optics – spectroscopic, diffraction and optical study</strong></h5>
<ul>
<li>IL 10: I. NĚMEC</li>
</ul>
<h5><strong>11:40 – 12:10 Nanoscale infrared spectroscopy studies of cellulose</strong></h5>
<ul>
<li>IL 11: M. JOHNSON</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Nanoscale_infrared_spectroscopy_studies_of_cellulose_M_JOHNSON_9db07bbebc/SSJMM-Nanoscale-infrared-spectroscopy-studies-of-cellulose-(M.-JOHNSON).webp" alt="SSJMM: Nanoscale infrared spectroscopy studies of cellulose (M. JOHNSON)"/></p>
<h5><strong>12:10 – 12:30 Nanoscopic techniques related to molecular vibrations</strong></h5>
<ul>
<li>OL 21: P. MATĚJKA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Nanoscopic_techniques_related_to_molecular_vibrations_P_MATEJKA_b341065a12/SSJMM-Nanoscopic-techniques-related-to-molecular-vibrations-(P.-MATEJKA).webp" alt="SSJMM: Nanoscopic techniques related to molecular vibrations (P. MATĚJKA)"/></p>
<h5><strong>12:30 – 12:50 Chemistry at the nanoscale – AFM meets IR spectroscopy</strong></h5>
<ul>
<li>OL 22: J. HORÁK</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Chemistry_at_the_nanoscale_AFM_meets_IR_spectroscopy_J_HORAK_cb38dc0517/SSJMM-Chemistry-at-the-nanoscale-AFM-meets-IR-spectroscopy-(J.-HORAK).webp" alt="SSJMM: Chemistry at the nanoscale – AFM meets IR spectroscopy (J. HORÁK)"/></p>
<h5><strong>12:50 – 14:30 LUNCH</strong></h5>
<ul>
<li>MU RECTORATE</li>
</ul>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>SPECIATION ANALYSIS 2</strong></h4>
<ul>
<li>(Hall P31) CHAIR: DONARD</li>
</ul>
<h5><strong>14:30 – 15:00 Elemental speciation from metals, metalloids to non-metals in environmental science: successes and problems</strong></h5>
<ul>
<li>IL 12: J. FELDMANN</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Elemental_speciation_from_metals_metalloids_to_non_metals_in_environmental_science_successes_and_problems_J_FELDMANN_9dbfaea2bb/SSJMM-Elemental-speciation-from-metals-metalloids-to-non-metals-in-environmental-science-successes-and-problems-(J.-FELDMANN).webp" alt="SSJMM: Elemental speciation from metals, metalloids to non-metals in environmental science - successes and problems (J. FELDMANN)"/></p>
<h5><strong>15:00 – 15:20 Arsenic and rice (Oryza sativa L.)</strong></h5>
<ul>
<li>OL 23: A. RAAB</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Arsenic_and_rice_Oryza_sativa_L_A_RAAB_28784318da/SSJMM-Arsenic-and-rice-(Oryza-sativa-L.)-(A.-RAAB).webp" alt="SSJMM: Arsenic and rice (Oryza sativa L.) (A. RAAB)"/></p>
<h5><strong>15:20 – 15:40 Comparison of mobile phases for cation exchange HPLC-ICP OES</strong></h5>
<ul>
<li>OL 24: A. ORLOWSKA</li>
</ul>
<h5><strong>15:40 – 16:00 Selenium transformation by food-grade microorganisms</strong></h5>
<ul>
<li>OL 25: A. KAŇA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Selenium_transformation_by_food_grade_microorganisms_A_KANA_a22d7ecbd5/SSJMM-Selenium-transformation-by-food-grade-microorganisms-(A.-KANA).webp" alt="SSJMM: Selenium transformation by food-grade microorganisms (A. KAŇA)"/></p>
<h5><strong>16:00 – 16:20 Determination of metals and metalloids species using self-designed hyphenated systems based on detection by optical emission spectrometry</strong></h5>
<ul>
<li>OL 26: J. PROCH</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Determination_of_metals_and_metalloids_species_using_self_designed_hyphenated_systems_based_on_detection_by_optical_emission_spectrometry_J_PROCH_1fe4bb7b75/SSJMM-Determination-of-metals-and-metalloids-species-using-self-designed-hyphenated-systems-based-on-detection-by-optical-emission-spectrometry-(J.-PROCH).webp" alt="SSJMM: Determination of metals and metalloids species using self-designed hyphenated systems based on detection by optical emission spectrometry (J. PROCH)"/></p>
<h5><strong>16:20 – 16:40 Mass balance analyses of PFAS in soil, snowmelt and ski waxes</strong></h5>
<ul>
<li>OL 27: V. MUELLER</li>
</ul>
<h5><strong>16:45 – 18:45 POSTER SESSION</strong></h5>
<ul>
<li>AUDITORIUM OF FACULTY OF SOCIAL STUDIES</li>
<li><a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/List_of_Posters_ESAS_CSSC_2022_final_044f000ec5/List_of_Posters_ESAS_CSSC_2022_final.pdf"><strong>List of Posters ESAS-CSSC 2022</strong></a></li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_POSTER_SESSION_Day_2_cf30f04d44/SSJMM-POSTER-SESSION-(Day-2).webp" alt="SSJMM: POSTER SESSION (Day 2)"/></p>
<h3><strong>PARALLEL SESSION</strong></h3>
<h4><strong>LUMINISCENCE SPECTROSCOPY</strong></h4>
<ul>
<li>(Hall P51) CHAIR: NĚMEC</li>
</ul>
<h5><strong>14:30 – 15:00 Inorganic nanoluminophores containing Ln ions and their multifunctionalized systems focused on sensor &amp; analytical applications</strong></h5>
<ul>
<li>IL 13: S. LIS</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Inorganic_nanoluminophores_containing_Ln_ions_and_their_multifunctionalized_systems_focused_on_sensor_and_analytical_applications_S_LIS_eacef96a49/SSJMM-Inorganic-nanoluminophores-containing-Ln-ions-and-their-multifunctionalized-systems-focused-on-sensor-and-analytical-applications-(S.-LIS).webp" alt="SSJMM: Inorganic nanoluminophores containing Ln ions and their multifunctionalized systems focused on sensor &amp; analytical applications (S. LIS)"/></p>
<h5><strong>15:00 – 15:30 Ultrafast time-resolved probes of chirality during photochemical reactions</strong></h5>
<ul>
<li>IL 14: V. SVOBODA</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Ultrafast_time_resolved_probes_of_chirality_during_photochemical_reactions_V_SVOBODA_721c3d23e7/SSJMM-Ultrafast-time-resolved-probes-of-chirality-during-photochemical-reactions-(V.-SVOBODA).webp" alt="SSJMM: Ultrafast time-resolved probes of chirality during photochemical reactions (V. SVOBODA)"/></p>
<h5><strong>15:30 – 15:50 FTIR-FPA and FTIR-QCL microscopy: Breakthrough techniques in infrared imaging</strong></h5>
<ul>
<li>OL 28: D. MATOUŠEK</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_FTIR_FPA_and_FTIR_QCL_microscopy_Breakthrough_techniques_in_infrared_imaging_D_MATOUSEK_5b3cc7da3c/SSJMM-FTIR-FPA-and-FTIR-QCL-microscopy-Breakthrough-techniques-in-infrared-imaging-(D.-MATOUSEK).webp" alt="SSJMM: FTIR-FPA and FTIR-QCL microscopy: Breakthrough techniques in infrared imaging (D. MATOUŠEK)"/></p>
<h5><strong>15:50 – 16:10 The optical chemosensor array for analysis of halides or small biological molecules</strong></h5>
<ul>
<li>OL 29: P. LUBAL</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_The_optical_chemosensor_array_for_analysis_of_halides_or_small_biological_molecules_P_LUBAL_3a8d1ab6b0/SSJMM-The-optical-chemosensor-array-for-analysis-of-halides-or-small-biological-molecules-(P.-LUBAL).webp" alt="SSJMM: The optical chemosensor array for analysis of halides or small biological molecules (P. LUBAL)"/></p>
<h5><strong>16:45 – 18:45 POSTER SESSION</strong></h5>
<ul>
<li>AUDITORIUM OF FACULTY OF SOCIAL STUDIES</li>
<li><a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/List_of_Posters_ESAS_CSSC_2022_final_044f000ec5/List_of_Posters_ESAS_CSSC_2022_final.pdf"><strong>List of Posters ESAS-CSSC 2022</strong></a></li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Conference_partners_ESAS_CSSC_2022_Pragolab_Thermo_Scientific_Merici_technika_Morava_Bruker_8e2a7377fe/SSJMM-Conference-partners-ESAS-CSSC-2022-(Pragolab-Thermo-Scientific-Merici-technika-Morava-Bruker).webp" alt="SSJMM: Conference partners ESAS-CSSC 2022 (Pragolab/Thermo Scientific, Měříci technika Morava/Bruker)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Conference_partners_ESAS_CSSC_2022_HPST_Agilent_Technologies_bca1f2466b/SSJMM-Conference-partners-ESAS-CSSC-2022-(HPST-Agilent-Technologies).webp" alt="SSJMM: Conference partners ESAS-CSSC 2022 (HPST/Agilent Technologies)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Conference_partners_ESAS_CSSC_2022_Shimadzu_Optik_instruments_Bruker_5ce1beb36d/SSJMM-Conference-partners-ESAS-CSSC-2022-(Shimadzu-Optik-instruments-Bruker).webp" alt="SSJMM: Conference partners ESAS-CSSC 2022 (Shimadzu, Optik instruments/Bruker)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Conference_partners_ESAS_CSSC_2022_Specion_Horiba_Chrom_Spec_4c105d6fdd/SSJMM-Conference-partners-ESAS-CSSC-2022-(Specion-Horiba-ChromSpec).webp" alt="SSJMM: Conference partners ESAS-CSSC 2022 (Specion/Horiba, ChromSpec)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SSJMM_Conference_partners_ESAS_CSSC_2022_Analytika_Perkin_Elmer_77ca8cffb3/SSJMM-Conference-partners-ESAS-CSSC-2022-(Analytika-PerkinElmer).webp" alt="SSJMM: Conference partners ESAS-CSSC 2022 (Analytika, PerkinElmer)"/></p>


ESAS-CSSC 2022 (Day 2)

<p>The invitation to give plenary lecture was accepted by:</p>
<ul>
<li><strong>Karl Mechtler</strong> (Research Institute of Molecular Pathology, Vienna)</li>
<li><strong>Carlos Cordeiro</strong> (Universidade de Lisboa)</li>
<li><strong>Berndt Wollscheid</strong> (ETH Zurich)</li>
</ul>
<h3><strong>Program CEEPC/IPM/CMSC 2022</strong></h3>
<blockquote>
<p><a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/abstractbook_2022_3bec805e4e/abstractbook_2022.pdf"><strong>Complete program including abstracts for download</strong></a></p>
</blockquote>
<h3><strong>Thursday September 29, 2022</strong></h3>
<h5><strong>8:30 - 10:00 Registration</strong></h5>
<h5><strong>10:00 - 10:10 Opening of the 16th CEEPC, 8th IPM and 10th CMSC</strong></h5>
<h4><strong>10:10 - 11:00 Plenary lecture I: New insights through Single-Cell Proteomics</strong> (Chairperson: Petr Novák)</h4>
<ul>
<li>Karl Mechtler</li>
</ul>
<h5><strong>11:00 - 11:20 Coffee break</strong></h5>
<h4><strong>11:20 - 13:00 Session I - Method Development</strong></h4>
<ul>
<li>Chairperson: Katarina Davalieva</li>
</ul>
<h5><strong>11:20 - 11:40 Identification of potential cell-surface targets and druggable enzymes in human pheochromocytoma and paraganglioma using classical and membrane-targeting proteomic approaches</strong></h5>
<ul>
<li>Ondřej Vít</li>
</ul>
<h5><strong>11:40 - 12:00 Global SEC-PCP-SILAC mapping reveals protein complexes mediating NF-κB activation in breast cancer</strong></h5>
<ul>
<li>Petr Lapčík</li>
</ul>
<h5><strong>12:00 - 12:20 Determination of changes in the milk proteome in production and storage of kefir using mass spectrometry-based omics approaches</strong></h5>
<ul>
<li>Haci Mehmet Kayili</li>
</ul>
<h5><strong>12:20 - 12:40 Non-Immunoaffinity Extraction of Intact Proteins from Biological Fluids and Their Analysis by Liquid Chromatography – Triple Quadrupole Mass Spectrometry</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<h5><strong>12:40 - 13:00 Advancing Cyclic Ion Mobility Mass Spectrometry Methods for Studying Biomolecules: Towards the Conformational Dynamics of Mega Dalton Protein Aggregates</strong></h5>
<ul>
<li>Adam Pruška</li>
</ul>
<h5><strong>13:00 - 15:30 Lunch + Poster session I (Odd-numbered posters)</strong></h5>
<h4><strong>15:30 - 16:30 Company Workshop – Bruker Daltonics</strong></h4>
<h5><strong>The latest proteomics development at Bruker</strong></h5>
<ul>
<li>Gary Kruppa</li>
</ul>
<h5><strong>Clinical (phospho)proteomics with TIMS and PASEF</strong></h5>
<ul>
<li>Florian Meier</li>
</ul>
<h5><strong>16:30 - 16:50 Coffee break</strong></h5>
<h4><strong>16:50 - 17:30 Short Talks</strong></h4>
<ul>
<li>Chairperson: Petr Man</li>
</ul>
<h5><strong>Detection of C. difficile Toxin B by biomolecule-modified chips and mass spectrometry</strong></h5>
<ul>
<li>Josef Dvořák</li>
</ul>
<h5><strong>Optimized HDX-MS workflow for antibody structure monitoring by HDX-MS</strong></h5>
<ul>
<li>Zuzana Kalaninová</li>
</ul>
<h5><strong>RPLC-UV and HILIC-UV characterisation of on-site produced Ramucirumab-DTPA immunoconjugate</strong></h5>
<ul>
<li>Denis Naplekov</li>
</ul>
<h5><strong>Collision energy setting in proteomics and glycoproteomics: From individual species to a practical perspective</strong></h5>
<ul>
<li>Agnes Revesz</li>
</ul>
<h5><strong>Novel circulating biomarkers of biventricular heart failure</strong></h5>
<ul>
<li>Matěj Běhounek</li>
</ul>
<h5><strong>Comprehensive metabolomic and lipidomic study of tauopathy and Alzheimer&#39;s disease patients</strong></h5>
<ul>
<li>Dana Dobešová</li>
</ul>
<h5><strong>Overlooked oligomerization process in Azurin, a model metalloprotein</strong></h5>
<ul>
<li>Roman Tuzhilkin</li>
</ul>
<h4><strong>17:30 - 18:00 Josef Chmelik Award</strong></h4>
<h5><strong>18:00 - 19:30 Guided tour to the conference dinner</strong></h5>
<h4><strong>19:30 - 23:00 Conference dinner and sightseeing boat trip</strong></h4>
<blockquote>
<h3><strong>Sponsors</strong></h3>
</blockquote>
<blockquote>
<ul>
<li>Bruker Daltonics</li>
<li>Amedis</li>
<li>Merck</li>
<li>Beckman coulter</li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters Corporation</strong></a></li>
</ul>
</blockquote>
<blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Sponsors_CEEPC_IPM_CMSC_2022_6f46a9b213/CSHS-Sponsors-CEEPC-IPM-CMSC-2022.webp" alt="ČSHS: Sponsors: CEEPC/IPM/CMSC 2022"/></p>
</blockquote>
<h3><strong>Friday September 30, 2022</strong></h3>
<h4><strong>8:50 - 9:40 Plenary lecture II: An FT-ICR-MS metabolomic journey</strong> (Chairperson: Michael Volný)</h4>
<ul>
<li>Carlos Cordeiro</li>
</ul>
<h4><strong>9:40 - 11:20 Session II - Novel Instrumentation and approaches</strong></h4>
<ul>
<li>Chairperson: Lászlo Drahos</li>
</ul>
<h5><strong>9:40 - 10:00 In a flash of light: Native mass spectrometry for single particle imaging with X ray free electron lasers</strong></h5>
<ul>
<li>Alan Kádek</li>
</ul>
<h5><strong>10:00 - 10:20 Qualitative and quantitative advancements of QTOF performance: the SCIEX ZenoTOF 7600 system</strong></h5>
<ul>
<li>Volker Kruft</li>
</ul>
<h5><strong>10:20 - 10:40 Synergistic success of proteo-genomics in profiling human tear fluid for disease biomarkers using a novel high sensitivity Immunoassay</strong></h5>
<ul>
<li>Suresh Jivan Gadher</li>
</ul>
<h5><strong>10:40 - 11:00 Back to Basics with Antibodies</strong></h5>
<ul>
<li>Stanislav Kukla</li>
</ul>
<h5><strong>11:00 - 11:20 Two-dimensional mass spectrometry for top-down analysis and structural characterization of proteins</strong></h5>
<ul>
<li>Maria van Agthoven</li>
</ul>
<h5><strong>11:20 - 11:40 Coffee break</strong></h5>
<h5><strong>11:40 - 13:00 Session Session III - MS and SW tools</strong></h5>
<ul>
<li>Chairperson: Eva Csosz</li>
</ul>
<h5><strong>11:40 - 12:00 Rapid ion mobility-resolved phosphoproteomics with dia-PASEF</strong></h5>
<ul>
<li>Florian Meier</li>
</ul>
<h5><strong>12:00 - 12:20 Prediction of Intact N-Glycopeptide Retention Time Windows in Hydrophilic Interaction Liquid Chromatography</strong></h5>
<ul>
<li>Tomáš Ječmen</li>
</ul>
<h5><strong>12:20 - 12:40 Quantification of small molecules in liquid chromatography/mass spectrometry datasets by CycloBranch</strong></h5>
<ul>
<li>Jiří Novák</li>
</ul>
<h5><strong>12:40 - 13:00 Mass spectrometric analysis of intact proteins: the dark side of deconvolution</strong></h5>
<ul>
<li>Lászlo Drahos</li>
</ul>
<h5><strong>13:00 - 15:30 Lunch + Poster session II (Even-numbered posters)</strong></h5>
<h4><strong>15:30 - 17:30 Session IV - Applications in Biomedicine 1</strong></h4>
<ul>
<li>Chairperson: Maria van Agthoven</li>
</ul>
<h5><strong>15:30 - 15:50 Identification of potential biomarkers for azoospermia by human testis proteomic analysis</strong></h5>
<ul>
<li>Katarina Davalieva</li>
</ul>
<h5><strong>15:50 - 16:10 Proteotype classification of renal cell carcinoma for prognosis and therapy response</strong></h5>
<ul>
<li>Pavel Bouchal</li>
</ul>
<h5><strong>16:10 - 16:30 Stability Study of Triacetylfusarinine C and Gliotoxin in bodily fluids</strong></h5>
<ul>
<li>Jiří Houšť</li>
</ul>
<h5><strong>16:30 - 16:50 MALDI-TOF mass spectrometry: A powerful method for blood meal identification in insect vectors</strong></h5>
<ul>
<li>Petr Halada</li>
</ul>
<h5><strong>16:50 - 17:10 Characterization of glycosphingolipids in iPSC-derived cerebral organoids and its application in neurodegenerative disorders</strong></h5>
<ul>
<li>Durga Jha</li>
</ul>
<h5><strong>17:10 - 17:30 Redox resetting by modulation of redox sensor Kelch-like ECH-associated protein 1 restores leukemic cells sensitivity to Azacytidine</strong></h5>
<ul>
<li>Kristýna Pimková</li>
</ul>
<h4><strong>17:30 - 18:10 <a href="https://lcms.cz/articles?search=zdenek+herman">Zdeněk Herman</a> Award presented by Resonance Foundation and presentation of the winning thesis</strong></h4>
<h5><strong>18:10 - 18:30 Coffee break</strong></h5>
<h4><strong>18:30 - 19:00 General assembly of the CSMS</strong></h4>
<h3><strong>Saturday October 1, 2022</strong></h3>
<h4><strong>8:50 - 10:50 Session V - Applications in Biomedicine 2</strong></h4>
<ul>
<li>Chairperson: Haci Mehmet Kayili</li>
</ul>
<h5><strong>8:50-9:10 Proteomic Analysis of Hepatitis B Virus</strong></h5>
<ul>
<li>Martin Hubálek</li>
</ul>
<h5><strong>9:10-9:30 The single amino acid substitutions in Mason-Pfizer Monkey Virus matrix protein modulate its proteolytic cleavage rate</strong></h5>
<ul>
<li>Jakub Sýs</li>
</ul>
<h5><strong>9:30-9:50 Cardiac alarmins as residual risk markers of atherosclerosis under lipid-lowering therapy</strong></h5>
<ul>
<li>Viorel-Iulian Suica</li>
</ul>
<h5><strong>9:50-10:10 Cervical mucus – a non-invasive uterine biomarker source?</strong></h5>
<ul>
<li>Tomáš Oždian</li>
</ul>
<h5><strong>10:10-10:30 High-Throughput µLC-MS/MS Lipidomics of 3D In Vitro Disease Models to Investigate Lipid Dysregulation</strong></h5>
<ul>
<li>Darshak Gadara</li>
</ul>
<h5><strong>10:30-10:50 Multi-omics network analysis in atherosclerosis reveals mechanisms driving the progression towords complicated lesion</strong></h5>
<ul>
<li>Eva Csosz</li>
</ul>
<h5><strong>10:50 - 11:10 Coffee break</strong></h5>
<h4><strong>11:10 - 12:00 Plenary lecture III: Light-mediated discovery of surfaceome nanoscale organization and inter-cellular receptor interaction networks</strong> (Chairperson: Pavel Bouchal)</h4>
<ul>
<li>Bernd Wollscheid</li>
</ul>
<h4><strong>12:00 - 12:20 Poster prize, Closing remarks</strong></h4>
<h5><strong>12:20 - 13:30 Lunch</strong></h5>


Conference CEEPC/IPM/CMSC 2022 (Program)

<h3><strong>Program CEEPC/IPM/CMSC 2022</strong></h3>
<blockquote>
<p><a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/abstractbook_2022_3bec805e4e/abstractbook_2022.pdf"><strong>Complete program including abstracts for download</strong></a></p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Suresh_Jivan_Gadher_cb291006b9/CSHS-Konference-CEEPC-IPM-CMSC-2022-(Suresh-Jivan-Gadher).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 (Suresh Jivan Gadher)"/></p>
<h3><strong>Thursday September 29, 2022</strong></h3>
<h5><strong>8:30 - 10:00 Registration</strong></h5>
<h5><strong>10:00 - 10:10 Opening of the 16th CEEPC, 8th IPM and 10th CMSC</strong></h5>
<h4><strong>10:10 - 11:00 Plenary lecture I: New insights through Single-Cell Proteomics</strong> (Chairperson: Petr Novák)</h4>
<ul>
<li>Karl Mechtler</li>
</ul>
<p>The analysis of single cell proteomes has recently become a viable complement to transcriptomics and genomics studies. Proteins are the main driver of cellular functionality and mRNA levels are often an unreliable proxy of such. Therefore, the global analysis of the proteome is essential to study cellular identities. Both multiplexed and label-free mass spectrometry-based approaches with single cell resolution have lately attributed surprising heterogeneity to believed homogenous cell populations. Even though specialized experimental designs and instrumentation have demonstrated remarkable advances, the efficient sample preparation of single cells still lacks behind.</p>
<p>Here, we introduce the proteoCHIP, optimized for multiplexed single cell proteomics sample preparation at surprising sensitivity and throughput. Sample processing using the cellenONE® robot, allows to reduce final sample volumes to low nanoliters submerged in a hexadecane layer simultaneously eliminating error prone manual sample handling and overcoming evaporation. This results in around 1,500 protein groups per analytical run at remarkable reporter ion signal to noise while reducing or eliminating the carrier proteome. We identified close to 2,600 proteins across 170 multiplexed single cells from two highly similar human cell types. This dedicated loss-less workflow allows to distinguish in vitro co-differentiated cell types of self-organizing cardiac organoids based on indicative markers across 150 single cells. In-depth characterization revealed enhanced cellular motility of endothelial cells and acute myocardium sarcomere organization in cardiomyocytes.</p>
<p>In addition, we evolved a robust and sensitive one-pot label free single cell workflow. By working in standard 384 well plates and compatibility with both, cell sorting in the cellenONE® or using alternatives like a FACS device the need for specialized equipment is obsolete making this workflow easy to use, cheap and accessible to a broader community. By keeping the sample in a hydrated state during proteolytic digestion and addition of DMSO for storage we improved recovery of hydrophobic peptides and boosted ID numbers to more than 1000 proteins from a single cell without match between runs.<br/>In conclusion, our versatile, and semi-automated sample preparation workflows have not only proven to be easily adoptable but are also sufficiently sensitive to drive biological applications of single cell proteomics.</p>
<h5><strong>11:00 - 11:20 Coffee break</strong></h5>
<blockquote>
<h3><strong>Sponsors</strong></h3>
</blockquote>
<blockquote>
<ul>
<li>Bruker Daltonics</li>
<li>Amedis</li>
<li>Merck</li>
<li>Beckman coulter</li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters Corporation</strong></a></li>
</ul>
</blockquote>
<blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Sponsors_CEEPC_IPM_CMSC_2022_6f46a9b213/CSHS-Sponsors-CEEPC-IPM-CMSC-2022.webp" alt="CMSC: Sponsors: CEEPC/IPM/CMSC 2022"/></p>
</blockquote>
<h4><strong>11:20 - 13:00 Session I - Method Development</strong></h4>
<ul>
<li>Chairperson: Katarina Davalieva</li>
</ul>
<h5><strong>11:20 - 11:40 Identification of potential cell-surface targets and druggable enzymes in human pheochromocytoma and paraganglioma using classical and membrane-targeting proteomic approaches</strong></h5>
<ul>
<li>Ondřej Vít</li>
</ul>
<p>Integral membrane proteins (IMPs) represent optimal drug targets but are under-represented in standard proteomic analyses due to their amphipathy, lack of trypsin cleavage sites, and low expression levels. To identify tumor-upregualted IMPs and druggable enzymes in rare neuroendocrine tumors – pheochromocytoma and paraganglioma (PPGL), we combined three approaches: 1) hpTC method, where the identification of IMPs is based on their hydrophobic alpha-helices, isolated by proteolytic shaving and re-cleaved with CNBr; 2) two glycocapture methods - lectin entrapment on ultrafilters (N-glyco-FASP), and solid-phase enrichment with hydrazide chemistry (SPEG); and 3) the classical detergent-trypsin approach.<br/>We focused mainly on the high-risk tumors belonging to the so-called cluster 1, characterized by mutations in genes related to citric acid cycle and hypoxia, such as SDHB, VHL, and EPAS1). The classical trypsin-based proteomic approach pointed us toward upregulated soluble druggable enzymes (autotaxin, SHMT2, and Arginase 2) and upregulated cell surface IMPs (including CD146 and CD171). The glycopeptide enrichment approach provided additional potential cell surface targets (CD39), and the hpTC method provided additional IMP targets (e.g., anoctamin-1).<br/>The above-mentioned IMPs and soluble enzymes have all been previously shown to be upregulated in several human cancers and to affect tumor progression directly. Their marked upregulation was confirmed by specific antibodies. Together, this makes these molecules promising candidates for drug targets and/or proteins enabling sensitive tumor imaging.</p>
<h5><strong>11:40 - 12:00 Global SEC-PCP-SILAC mapping reveals protein complexes mediating NF-κB activation in breast cancer</strong></h5>
<ul>
<li>Petr Lapčík</li>
</ul>
<p>NF-κB has essential role in immune response and is associated with lymph node metastasis of luminal A breast tumors (1). Analysis of protein interactome and its changes in response to NF-κB modulation could uncover pro-metastatic mechanisms related to NF-κB. We apply metabolic isotope labeling SILAC, size exclusion chromatography (SEC) and protein correlation profiling (PCP) (2) to construct a network of interactome rearrangement in response to NF-κB modulation in MCF-7 breast cancer cells.</p>
<p>We generated two co-fractionation datasets consisting of 80 fractions from SILAC-labeled and 80 fractions from label-free native MCF-7 lysates with inhibited or native NF-κB activity. LC-MS/MS analysis of SEC fractions using Orbitrap Lumos and Bruker Impact II mass spectrometers quantified 3308 and 5460 protein groups in SILAC and label-free datasets, respectively (FDR = 0.01). Interactome reconstruction using PrinCE (3) detected 7568 interactions among 1520 proteins. Co-elution of subunits of known complexes, such as ribosome, proteasome and MCM, was observed. Modulation of NF-κB was linked to interactome changes of proteins involved in immune response, cell cycle and DNA replication. NF-κB factor RELA interacted with proteins co-eluting with activators of NF-κB and these interactions were modulated by NF-κB inhibition.</p>
<p>Our interaction network represents a complex insight into dynamics of MCF-7 protein interactome associated with NF-κB pathway and could serve as a basis for future studies characterizing NF-κB in breast cancer.</p>
<h5><strong>12:00 - 12:20 Determination of changes in the milk proteome in production and storage of kefir using mass spectrometry-based omics approaches</strong></h5>
<ul>
<li>Haci Mehmet Kayili</li>
</ul>
<p>Kefir is an important food source based in the Caucasus region. The beneficial effects of kefir on human health are indicated in the literature. The mechanisms underlying these critical properties of kefir have not yet been elucidated. This study aims to examine the changes in milk protein profiles during kefir production and storage by using mass spectrometry-based-omics approaches. In addition, the detection of kefir-based proteins that transfer to milk from kefir microflora was investigated within the scope of the study. First, Kefir production was carried out. During the production and storage of kefir, kefir samples were taken from the periods determined in the study, and milk proteins were extracted. Peptides were produced using Lys-C and trypsin enzymes by a classical proteomics approach. Afterward, the fractionation of the peptides was carried out. Peptide-containing samples were analyzed by nLC-QExactive-Plus mass spectrometry. The data obtained as a result of the analysis were processed with the Maxquant software, and statistical analyzes were performed. According to the results obtained, significant changes were found in the milk proteome in 3 proteins between 0-12 hours, 39 proteins between 0-24 hours, 41 proteins between 0-7 days, and finally, 42 proteins between 0-28 days. From the 24th hour, a significant change was observed in 23 proteins. In the analysis, it was determined in the study that 398 kefir-based proteins were released into the milk. It is anticipated that these results will provide valuable contributions to the literature in terms of showing the changes in the milk proteome in kefir production.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Determination_of_changes_in_the_milk_proteome_in_production_and_storage_of_kefir_using_mass_spectrometry_based_omics_approaches_Haci_Mehmet_Kayili_866576d951/CSHS-Konference-CEEPC-IPM-CMSC-2022-Determination-of-changes-in-the-milk-proteome-in-production-and-storage-of-kefir-using-mass-spectrometry-based-omics-approaches-(Haci-Mehmet-Kayili).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Determination of changes in the milk proteome in production and storage of kefir using mass spectrometry-based omics approaches (Haci Mehmet Kayili)"/></p>
<h5><strong>12:20 - 12:40 Non-Immunoaffinity Extraction of Intact Proteins from Biological Fluids and Their Analysis by Liquid Chromatography – Triple Quadrupole Mass Spectrometry</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<p>One of the crucial steps in quantitation of intact proteins from complex biological matrices is, except their reliable analysis, also sample preparation to achieve sufficient specificity and sensitivity. Commonly used immunoaffinity-based methods are characterized by their superior selectivity, although this can be a drawback if simultaneous analysis of multiple different proteins from a single sample is required. In our work, we developed non-immunoaffinity sample preparation based on a generally widely affordable microelution solid phase extraction for eleven model intact proteins (5.5 – 29 kDa) with various isoelectric points (4.5 - 11.3). Extracted intact proteins were analysed by reversed-phase liquid chromatography coupled with a triple quadrupole mass spectrometer operated in a multiple reaction monitoring (MRM) mode. Reversed-phase separations were performed on the Restek wide-pore Viva C4 column, as mobile phases served water and acetonitrile acidified by 0.1% difluoroacetic acid and 0.2% formic acid. The best recoveries for most of the selected proteins were obtained by using the HLB stationary phase. 1% trifluoroacetic acid and 0.2% Triton X-100 were used as efficient pretreatment reagents to release interactions between the proteins and biological matrix. Multiple sample loading was found out to be essential to obtain recoveries &gt;65% in urine for all targeted proteins (up to 30kDa) and &gt;50% in serum/plasma for most of the proteins. Limits of quantitation in biological matrices were in the range 2 - 1200 ng/mL, corresponding to 0.23 - 97.6 nM.</p>
<h5><strong>12:40 - 13:00 Advancing Cyclic Ion Mobility Mass Spectrometry Methods for Studying Biomolecules: Towards the Conformational Dynamics of Mega Dalton Protein Aggregates</strong></h5>
<ul>
<li>Adam Pruška</li>
</ul>
<p>Native mass spectrometry is a powerful tool for the analysis of non-covalent complexes. When coupled with high-resolution ion mobility, this technique can be used to investigate the conformational changes induced in said complexes by different solution or gas-phase conditions. In this study, we describe how a new generation high-resolution ion mobility instrument equipped with a cyclic ion mobility cell can be utilized for the analysis of large biomolecular systems, including temperature-induced protein aggregates of masses greater than 1.5 MDa, as well as a 63 kDa oligonucleotide complex. The effects of and the interplay between the voltages applied to the different components of the cyclic ion mobility spectrometry system on ion transmission and arrival time distribution were demonstrated using biomolecules covering the m/z range 2 000 to 10 000. These data were used to establish a theoretical framework for achieving the best separation on the cyclic ion mobility system. Finally, the cyclic ion mobility mass spectrometer was coupled with a temperature-controlled electrospray ionization source to investigate high-mass protein aggregation. This analysis showed that it was possible to continuously monitor the change in abundance for several conformations of MDa aggregates with increasing temperature. This work significantly increases the range of biomolecules that can be analyzed by both cyclic ion mobility and temperature-controlled electrospray ionization mass spectrometry, providing new possibilities for high-resolution ion mobility analysis.</p>
<h5><strong>13:00 - 15:30 Lunch + Poster session I (Odd-numbered posters)</strong></h5>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Posterova_sekce_I_Postery_liche_aecf40bb37/CSHS-Konference-CEEPC-IPM-CMSC-2022-Posterova-sekce-I-(Postery-liche).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Poster session I (Odd-numbered posters)"/></p>
<h4><strong>15:30 - 16:30 Company Workshop – Bruker Daltonics</strong></h4>
<h5><strong>The latest proteomics development at Bruker</strong></h5>
<ul>
<li>Gary Kruppa</li>
</ul>
<h5><strong>Clinical (phospho)proteomics with TIMS and PASEF</strong></h5>
<ul>
<li>Florian Meier</li>
</ul>
<h5><strong>16:30 - 16:50 Coffee break</strong></h5>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Petr_Novak_a_Michael_Volny_8288df35cd/CSHS-Konference-CEEPC-IPM-CMSC-2022-Petr-Novak-a-Michael-Volny.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Petr Novák and Michael Volný"/></p>
<h4><strong>16:50 - 17:30 Short Talks</strong></h4>
<ul>
<li>Chairperson: Petr Man</li>
</ul>
<h5><strong>Detection of C. difficile Toxin B by biomolecule-modified chips and mass spectrometry</strong></h5>
<ul>
<li>Josef Dvořák</li>
</ul>
<h5><strong>Optimized HDX-MS workflow for antibody structure monitoring by HDX-MS</strong></h5>
<ul>
<li>Zuzana Kalaninová</li>
</ul>
<h5><strong>RPLC-UV and HILIC-UV characterisation of on-site produced Ramucirumab-DTPA immunoconjugate</strong></h5>
<ul>
<li>Denis Naplekov</li>
</ul>
<h5><strong>Collision energy setting in proteomics and glycoproteomics: From individual species to a practical perspective</strong></h5>
<ul>
<li>Agnes Revesz</li>
</ul>
<h5><strong>Novel circulating biomarkers of biventricular heart failure</strong></h5>
<ul>
<li>Matěj Běhounek</li>
</ul>
<h5><strong>Comprehensive metabolomic and lipidomic study of tauopathy and Alzheimer&#39;s disease patients</strong></h5>
<ul>
<li>Dana Dobešová</li>
</ul>
<h5><strong>Overlooked oligomerization process in Azurin, a model metalloprotein</strong></h5>
<ul>
<li>Roman Tuzhilkin</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Den_1_62de462e8b/CSHS-Konference-CEEPC-IPM-CMSC-2022-Den-1.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Day 1"/></p>
<h4><strong>17:30 - 18:00 Josef Chmelik Award</strong></h4>
<h5><strong>18:00 - 19:30 Guided tour to the conference dinner</strong></h5>
<h4><strong>19:30 - 23:00 Conference dinner and sightseeing boat trip</strong></h4>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Slavnostni_vecere_a_vylet_na_lodi_249c7c3702/CSHS-Konference-CEEPC-IPM-CMSC-2022-Slavnostni-vecere-a-vylet-na-lodi.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Conference dinner and sightseeing boat trip"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Slavnostni_vecere_a_vylet_na_lodi_3_6452afa91a/CSHS-Konference-CEEPC-IPM-CMSC-2022-Slavnostni-vecere-a-vylet-na-lodi-3.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Conference dinner and sightseeing boat trip"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Slavnostni_vecere_a_vylet_na_lodi_2_42bb7f554d/CSHS-Konference-CEEPC-IPM-CMSC-2022-Slavnostni-vecere-a-vylet-na-lodi-2.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Conference dinner and sightseeing boat trip"/></p>


Conference CEEPC/IPM/CMSC 2022 (Day 1)

Conference CEEPC/IPM/CMSC 2022 (Day 2)

<h3><strong>Program CEEPC/IPM/CMSC 2022</strong></h3>
<blockquote>
<p><a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/abstractbook_2022_3bec805e4e/abstractbook_2022.pdf"><strong>Complete program including abstracts for download</strong></a></p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_2_aa28bf7c17/CSHS-Konference-CEEPC-IPM-CMSC-2022-2.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022"/></p>
<h3><strong>Saturday October 1, 2022</strong></h3>
<h4><strong>8:50 - 10:50 Session V – Applications in Biomedicine 2</strong></h4>
<ul>
<li>Chairperson: Haci Mehmet Kayili</li>
</ul>
<h5><strong>8:50-9:10 Proteomic Analysis of Hepatitis B Virus</strong></h5>
<ul>
<li>Martin Hubálek</li>
</ul>
<p>According to the World Health Organisation, an estimated 296 million people worldwide are infected with Hepatitis B virus (HBV). HBV is a small enveloped DNA virus from Hepadnaviridae family that persists in the infected hepatocytes by establishing an episomal covalently closed circular double-stranded DNA (cccDNA) genome containing four open reading frames (C, P, S and X). The ORFs largely overlap and encode multiple proteins using different in-frame start codons. The S ORF encodes three forms of surface envelope glycoproteins S-, M-, and L- HBs). The HBV preC-C gene gives rise to two different products translated from distinct mRNAs – core protein (HBc) and precore protein (HBe). Despite their high sequence similarity, these proteins exhibit different functions and subcellular localizations. The P ORF encodes viral polymerase, and the X ORF encodes regulatory HBx protein.</p>
<p>Mass Spectrometry department of IOCB has established a collaboration with the groups of Iva Pichova and Jan Weber from IOCB. This collaboration on proteomics experiments lead to the discovery of several important biological and biochemical findings including identification of interacting partners of HBc, HBx and HBe proteins or posttranslational modifications of HBc protein. The collaboration resulted in several publications. The proteomic analysis of individually expressed viral proteins has also captured out-of-frame products suggesting the possibility of frameshift or alternative internal initiation of translation. The presentation will highlight the proteomic analyses of HBV at IOCB</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Proteomic_Analysis_of_Hepatitis_B_Virus_Martin_Hubalek_a95dd6216f/CSHS-Konference-CEEPC-IPM-CMSC-2022-Proteomic-Analysis-of-Hepatitis-B-Virus-(Martin-Hubalek).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Proteomic Analysis of Hepatitis B Virus (Martin Hubálek)"/></p>
<h5><strong>9:10-9:30 The single amino acid substitutions in Mason-Pfizer Monkey Virus matrix protein modulate its proteolytic cleavage rate</strong></h5>
<ul>
<li>Jakub Sýs</li>
</ul>
<p>N-terminal domain of polyprotein Gag of Mason-Pfizer Monkey Virus (M-PMV) Matrix protein (MA) is naturally myristoylated on its N-terminus. When MA is a part of polyprotein Gag, the myristate moiety is buried inside hydrophobic pocket and exposed probably upon interaction with host cell plasma membrane (PM). This mechanism called myristoyl switch does not occur in M-PMV as readily as it does in HIV-1, suggesting that it may have an important role in M-PMV maturation by regulating the MA cleavage from Gag polyprotein.</p>
<p>To address this hypothesis, we have treated the myristoylated (myrMAPPHis) and nonmyristoylated MAPPHis (nonmyrMAPPHis) M PMV construct, bearing the cleavage site for M-PMV viral protease (Pr13), on artificial liposomes mimicking PM by Pr13 for several time points. In contrast to rapidly degraded nonmyrMAPPHis even without liposomes, the myrMAPPHis, surprisingly, become cleaved more frequently only after addition of liposomes indicating the possible exposure of myristate upon interaction with PM.</p>
<p>To support our findings, we have designed and examined also four mutants of MAPPHis M PMV with single amino acid substitutions with expectation to block or, conversely, facilitate the myristoyl switch by stabilization (A79V, A79L) or destabilization (I51A, I86A) of MA M PMV hydrophobic pocket. The mutants A79V and A79L were cleaved even less effectively than myrMAPPHis as well as the degradation of mutants I51A and I86A was faster compared to nonmyrMAPPHis. The different cleavage rates of proteins were confirmed also on structural level by using method of hydrogendeuterium exchange coupled with mass spectrometric detection. It shows that the protease cleavage site has different dynamics in proteins as a result of disruption/stabilisation of hydrophobic pocket.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_The_single_amino_acid_substitutions_in_Mason_Pfizer_Monkey_Virus_matrix_protein_modulate_its_proteolytic_cleavage_rate_Jakub_Sys_e300792938/CSHS-Konference-CEEPC-IPM-CMSC-2022-The-single-amino-acid-substitutions-in-Mason-Pfizer-Monkey-Virus-matrix-protein-modulate-its-proteolytic-cleavage-rate-(Jakub-Sys).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: The single amino acid substitutions in Mason-Pfizer Monkey Virus matrix protein modulate its proteolytic cleavage rate (Jakub Sýs)"/></p>
<h5><strong>9:30-9:50 Cardiac alarmins as residual risk markers of atherosclerosis under lipid-lowering therapy</strong></h5>
<ul>
<li>Viorel-Iulian Suica</li>
</ul>
<p>Background: The major cause in the initiation and progression of atherosclerosis are the high levels of low-density lipoproteins. Although statin treatment can effectively lower these levels, there is still a residual risk of cardiovascular events. We hypothesize that a specific panel of alarmins, a family of stress-sensing molecules, could indicate the persistence of silent atherosclerosis residual risk. </p>
<p>Methods: New Zeeland White rabbits were divided into: the control group (C) with standard diet, a group which received a high-fat diet for 12 weeks (Au) and a treated hyperlipidemic group, with a lipid diet for 8 weeks followed by standard diet and hypolipidemic treatment (atorvastatin and PCSK9 siRNA-inhibitor) for 4 weeks (Asi). We used the LTQ Orbitrap Velos Pro mass spectrometer to analyse the left ventricle lysates. The experiments were complemented by immunologic and genomic assays to corroborate the data.</p>
<p>Results: The hyperlipidemic diet determined a general alarmin up-regulation tendency over C group. A significant spectral abundance increase was measured for specific heat shock proteins, S100 family members, HMGB1 and Annexin A1. The hypolipidemic treatment demonstrated a reversed regulation trend, with non-significant spectral alteration over the C group for some identified alarmins.</p>
<p>Conclusion: Our study highlights the discriminating potential of alarmins in hyperlipidemia or following a hypolipidemic treatment.</p>
<h5><strong>9:50-10:10 Cervical mucus – a non-invasive uterine biomarker source?</strong></h5>
<ul>
<li>Tomáš Oždian</li>
</ul>
<p>Cervical mucus (CM) is a viscous fluid produced by cervical glands located in the myometrium of the uterine cervix. Usually, CM forms a plug between vagina and uterus. During the ovulation, CM starts to be more fluidic to allow the sperm to go through. During the ovulation, there is a good window for non-invasive sampling of the CM, originating from uterine derived tissues. The main hypothesis for CM proteomic study is to develop a method for CM sampling, processing and to answer if there are enough proteins in the CM for potential biomarker search. The resulting protocol consists of mucus dissolution, multi-enzyme digestion, and LC-MS proteomic analysis. The optimized approach allows to identify more than thousand proteins from every sample tested. The bioinformatic analysis reveals that the identified proteins are both intra and extra cellular and discovers the proteins with enriched expression in the broader female reproductive tract. To our best knowledge, we provide the most extensive proteomic characterization available.</p>
<h5><strong>10:10-10:30 High-Throughput µLC-MS/MS Lipidomics of 3D In Vitro Disease Models to Investigate Lipid Dysregulation</strong></h5>
<ul>
<li>Darshak Gadara</li>
</ul>
<p>Microflow liquid chromatography interfaced with mass spectrometry is increasingly applied for the high-throughput profiling of biological samples, as it demonstrated an acceptable tradeoff between sensitivity and reproducibility. However, a limited number of applications developed in lipidomics. This study introduces a robust, sensitive, and high-throughput μLC-MS/MS lipidomics workflow, applied in a differential lipidome study of APOE 3 and 4 phenotypes in iPSC-derived cerebral organoids (CO). First, we optimized the microbore column gradient conditions, injecting a mixture of 15 lipid standards on the UHPLC system coupled to a 6469 QQQ. Our μLC-MS/MS method allows quantitation of 351 lipid species from the single CO. The microflow lipidomics shows an average 3.7-fold increase in response compared to a conventional high-flow method. To benchmark the quantitative performance, 303 samples were acquired over 75 hours. The median coefficient of variation (CV) of 351 lipid species was 12.95%, only 26 lipid species show CV higher than 30%. We demonstrated that microflow separation coupled to three-stage quadruple MS substantially increases the sensitivity and allows for robust multiplexed lipid profiling (including cholesterol determination) in a single cerebral organoid. As a proof of concept, lipidomics analysis was performed for the lipid extract of APOE 3/3 and APOE 4/4 (n=10) CO samples. To our knowledge, this is the first systematic report to demonstrate the microflow LC-MS/MS allows for sensitive, high-throughput, and robust measurement of global lipidome, opening an avenue for routine application of microflow separation in the field of lipidomics.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_High_Throughput_LC_MS_MS_Lipidomics_of_3_D_In_Vitro_Disease_Models_to_Investigate_Lipid_Dysregulation_Darshak_Gadara_0935fe540d/CSHS-Konference-CEEPC-IPM-CMSC-2022-High-Throughput-LC-MS-MS-Lipidomics-of-3D-In-Vitro-Disease-Models-to-Investigate-Lipid-Dysregulation-(Darshak-Gadara).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: High-Throughput µLC-MS-MS Lipidomics of 3D In Vitro Disease Models to Investigate Lipid Dysregulation (Darshak Gadara)"/></p>
<h5><strong>10:30-10:50 Multi-omics network analysis in atherosclerosis reveals mechanisms driving the progression towords complicated lesion</strong></h5>
<ul>
<li>Eva Csosz</li>
</ul>
<p>From a biological point of view, it is crucial to understand the mechanisms laying behind atherosclerosis and its complicated forms. In the process of atherosclerosis, first, an atheromatous plaque builds up, which in some conditions can lead to the appearance of complicated lesions. The complicated lesion is characterized by ruptures on the atherosclerotic plaque surface and/or hemorrhage into the plaque. According to the WHO, hemorrhaged lesions, contribute to a considerable extent of deaths worldwide.</p>
<p>The main goal of our project was to perform a multi-omics study involving transcriptomics and proteomics data to get more insights into the pathophysiological processes dominating the complicated lesions.</p>
<p>Transcriptomic (RNAseq) and proteomic (label-free quantification) data were obtained by the analysis of artery samples originating from healthy arteries, atheromatous lesions, and complicated lesions. Both data types were examined separately by Ingenuity Pathway Analysis and later an overlay of the networks was achieved.</p>
<p>120 differentially expressed (DE) proteins and more than 4000 DE genes were detected. The deep analysis of the data could identify the proteins and transcripts characteristic of the complicated lesions and could reveal the pathways specific to atheroma and complicated lesions, respectively. The DE genes were arranged into 25 networks, while the DE proteins were into 12 networks. The lower number of DE proteins compared to the number of DE genes did not result in loss of information, indicating the power of the proteomics experiments. In spite of providing a lower number of DE items, the biological information achievable from proteomics experiments has equal value compared to transcriptomics data.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Multi_omics_network_analysis_in_atherosclerosis_reveals_mechanisms_driving_the_progression_towords_complicated_lesion_Eva_Csosz_aa54a2b04f/CSHS-Konference-CEEPC-IPM-CMSC-2022-Multi-omics-network-analysis-in-atherosclerosis-reveals-mechanisms-driving-the-progression-towords-complicated-lesion-(Eva-Csosz).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Multi-omics network analysis in atherosclerosis reveals mechanisms driving the progression towords complicated lesion (Eva Csosz)"/></p>
<h5><strong>10:50 - 11:10 Coffee break</strong></h5>
<h4><strong>11:10 - 12:00 Plenary lecture III:: Light-mediated discovery of surfaceome nanoscale organization and inter-cellular receptor interaction networks</strong> (Chairperson: Pavel Bouchal)</h4>
<ul>
<li>Bernd Wollscheid</li>
</ul>
<p>The molecular nanoscale organization of the surfaceome is a fundamental regulator of cellular signaling in health and disease. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would unlock theranostic opportunities to target protein communities and the possibility to engineer extracellular signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables the targeted elucidation of acute protein interactions on and in between living cells using light-controlled singlet oxygen generators (SOG). By using SOG-coupled antibodies, small molecule drugs, biologics and intact viral particles, we demonstrate the ability of LUX-MS to decode ligand receptor interactions across organisms and to discover surfaceome receptor nanoscale organization with direct implications for drug action. Furthermore, by coupling SOG to antigens we achieved light-controlled molecular mapping of intercellular signaling within functional immune synapses between antigen-presenting cells and CD8⁺ T cells providing insights into T cell activation with spatiotemporal specificity. LUX-MS based decoding of surfaceome signaling architectures thereby provides a molecular framework for the rational development of theranostic strategies.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Light_mediated_discovery_of_surfaceome_nanoscale_organization_and_inter_cellular_receptor_interaction_networks_Bernd_Wollscheid_019214ab5c/CSHS-Konference-CEEPC-IPM-CMSC-2022-Light-mediated-discovery-of-surfaceome-nanoscale-organization-and-inter-cellular-receptor-interaction-networks-(Bernd-Wollscheid).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Light-mediated discovery of surfaceome nanoscale organization and inter-cellular receptor interaction networks (Bernd Wollscheid)"/></p>
<h4><strong>12:00 - 12:20 Poster prize, Closing remarks</strong></h4>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Predavani_oceneni_konference_CEEPC_IPM_CMSC_2022_Ales_Kvasnicka_a_Michael_Volny_4c0967dd3b/CSHS-Predavani-oceneni-konference-CEEPC-IPM-CMSC-2022-(Ales-Kvasnicka-a-Michael-Volny).webp" alt="ČSHS: Conference prize CEEPC/IPM/CMSC 2022 (Aleš Kvasnička a Michael Volný)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Eu_PA_cestovni_granty_Nejlepsi_kratke_sdeleni_Kalaninova_Zuzana_a_Eva_Csosz_ef6a262eae/CSHS-EuPA-cestovni-granty-Nejlepsi-kratke-sdeleni-(Kalaninova-Zuzana-a-Eva-Csosz).webp" alt="ČSHS: EuPA travel grants: Short talk prize CEEPC/IPM/CMSC 2022 (Kalaninová Zuzana a Eva Csosz)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Vyhlaseni_nejlepsiho_posteru_Konference_CEEPC_IPM_CMSC_2022_Anton_Skriba_a_Michael_Volny_0010fccaaa/CSHS-Vyhlaseni-nejlepsiho-posteru-Konference-CEEPC-IPM-CMSC-2022-(Anton-Skriba-a-Michael-Volny).webp" alt="ČSHS: Poster prize CEEPC/IPM/CMSC 2022 (Anton Škríba a Michael Volný)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Eu_PA_cestovni_granty_Nejlepsi_poster_Jadeja_Siddharth_a_Eva_Csosz_dac2e7c761/CSHS-EuPA-cestovni-granty-Nejlepsi-poster-(Jadeja-Siddharth-a-Eva-Csosz).webp" alt="ČSHS: EuPA travel grants: Poster prize CEEPC/IPM/CMSC 2022 (Jadeja Siddharth a Eva Csosz)"/></p>
<h5><strong>12:20 - 13:30 Lunch</strong></h5>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Obcerstveni_44ad451fcc/CSHS-Konference-CEEPC-IPM-CMSC-2022-Obcerstveni.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Coffe break"/></p>


Conference CEEPC/IPM/CMSC 2022 (Day 3)

<p><strong>STARSS project is approaching its end after more than five years. A few more months remain before it will be concluded. The major objective of the project was to create a strong team of researchers enabling significant progress in the field of the separation science. The annual STARSS conferences have always been a good opportunity to bring people from several Czech and European institutions in Hradec Kralove where we have met face to face for a few days in order to inform each other about the progress in the research. It is clear that the focus of these conferences has been on the exchange in scientific information. However, an integral part of our previous conferences has also included informal meetings when we had time to talk about issues sometimes even completely outside of science perhaps with a drink and food. Social part of conferences is simply indispensable.</strong></p>
<p><strong>I trust that this conference will be at least as interesting as all the previous ones. Its program is rich and includes presentations by renowned “icons” of separation science from both abroad and the Czech Republic, as well as talks given by students and postdocs. Such “cross-pollination” will certainly be uplifting for all involved. I wish all those who attend this conference a pleasant stay in Hradec Králové and enjoyable conferencing.</strong></p>
<p><strong><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec">Prof. František Švec</a></strong></p>
<h3><strong>Program: 6th STARSS Conference on Separation Science</strong></h3>
<ul>
<li><a href="https://portal.faf.cuni.cz/getattachment/Projects/STARSS/Events/6th-STARSS-conference/Program/2022-STARSS-booklet.pdf.aspx?lang=en-GB"><strong>Conference booklet including speakers bio</strong></a></li>
</ul>
<h3><strong>DAY 1: Monday 17. 10. 2022</strong></h3>
<h5><strong>08:00 – 09:00 REGISTRATION</strong></h5>
<h5><strong>09:00 – 09:20 Welcoming address</strong></h5>
<ul>
<li>Prof. Petr Solich, Prof. František Švec, Prof. Lucie Nováková</li>
<li>Assoc. Prof. Jaroslav Roh (the dean of the Faculty of Pharmacy, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h4><strong>SESSION I: IMPORTANCE OF SEPARATION SCIENCE FOR CLINICAL APPLICATIONS</strong></h4>
<ul>
<li>Chairperson: Prof. František Švec</li>
</ul>
<h5><strong>09:20 – 10:00 L-01: UHPSFC/MS as a powerful tool for high-throughput lipidomic quantitation in cancer screening</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek">Prof. Michal Holčapek</a> (University of Pardubice, Czech Republic)</li>
</ul>
<h5><strong>10:00 – 10:40 L-02: Selected ion flow tube mass spectrometry, SIFT-MS, analyses of volatile metabolites in exhaled breath</strong></h5>
<ul>
<li>Prof. Patrik Španěl (J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic)</li>
</ul>
<h5><strong>10:40 – 11:10 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION II: BIOPHARMACEUTICALS</strong></h4>
<ul>
<li>Chairperson: Prof. Lucie Nováková</li>
</ul>
<h5><strong>11:10 – 11:50 L-03: Simplifying the characterization of complex biopharmaceutical products through the use of ultra-short columns or multi-isocratic elution mode</strong></h5>
<ul>
<li>Dr. Davy Guillarme (University of Geneva, Switzerland)</li>
</ul>
<h5><strong>11:50 – 12:20 L-04: Analysis of therapeutic proteins by multidimensional LC: How far can we go?</strong></h5>
<ul>
<li>Dr. Valentina D ́Atri (University of Geneva, Switzerland)</li>
</ul>
<h5><strong>12:20 – 12:40 L-05: pyroLC: online thermal decomposition of proteins as a tool for ultra-fast bottom-up proteomics</strong></h5>
<ul>
<li>Dr. Juraj Lenčo (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>12:40 – 13:00 L-06: RPLC-UV and HILIC-UV characterization of home-made ramucirumab-based antibody-drug conjugate</strong></h5>
<ul>
<li>Denis Naplekov (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<h4><strong>SESSION III: 2D SEPARATIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Jiří Urban</li>
</ul>
<h5><strong>14:00 – 14:40 L-07: What can we expect from LC x LC compared to conventional 1D-LC</strong></h5>
<ul>
<li>Prof. Sabine Heinisch (Université Claude Bernard Lyon 1, Lyon, France)</li>
</ul>
<h5><strong>14:40 – 15:20 L-08: Modelling of analyte profiles and band broadening generated by interface loops used in multi-dimensional liquid chromatography</strong></h5>
<ul>
<li>Prof. Ken Broeckhoven (Vrije Universiteit Brussel, Belgium)</li>
</ul>
<h5><strong>15:20 – 15:50 L-09: Off-line LC x SFC in the prospect of upgrading natural or recycled products</strong></h5>
<ul>
<li>Dr. Karine Faure (Université Claude Bernard Lyon 1, Lyon, France)</li>
</ul>
<h5><strong>15:50 – 16:20 L-10: Two-dimensional liquid chromatographic separations of complex samples of industrial products and intermediates</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Assoc. Prof. Petr Česla</strong></a> (University of Pardubice, Czech Republic)</li>
</ul>
<h5><strong>16:20 – 16:50 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION IV: IMPORTANCE OF STEROIDS</strong></h4>
<ul>
<li>Chairperson: Prof. Marcela Segundo</li>
</ul>
<h5><strong>16:50 – 17:30 L-11: An image of the future of steroid biochemistry</strong></h5>
<ul>
<li>Prof. Ruth Andrew (University of Edinburgh, UK)</li>
</ul>
<h5><strong>17:30 – 18:00 L-12: Ecdysterone metabolism: A holistic view on compound identification for doping control</strong></h5>
<ul>
<li>Prof. Maria Kristina Parr (Freie Universität Berlin, Berlin, Germany)</li>
</ul>
<h5><strong>18:00 – 18:30 L-13: New liquid chromatography-tandem mass spectrometry method for targeted profiling of steroids in mouse plasma</strong></h5>
<ul>
<li>Taťána Gazárková (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>18:30 – 19:30 Welcome drink</strong></h5>
<h3><strong>DAY 2: Tuesday 18. 10. 2022</strong></h3>
<h5><strong>08:00 – 09:00 REGISTRATION</strong></h5>
<h4><strong>SESSION V: SUPERCRITICAL FLUID CHROMATOGRAPHY AND EXTRACTIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Radim Kučera</li>
</ul>
<h5><strong>09:00 – 09:30 L-14: Supercritical fluid extraction and chromatography in life science applications</strong></h5>
<ul>
<li>Prof. Charlotta Turner (Lund University, Lund, Sweden)</li>
</ul>
<h5><strong>09:30 – 10:00 L-15: Supercritical fluid chromatography – tandem mass spectrometry for analysis of vitamin D metabolites in biological samples</strong></h5>
<ul>
<li>Dr. Margareta Sandahl (Lund University, Lund, Sweden)</li>
</ul>
<h5><strong>10:00 – 10:30 L-16: The effect of make-up solvent composition on responses in SFC-MS: Electrospray vs UniSpray</strong></h5>
<ul>
<li>Dr. Kateřina Plachká (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>10:30 – 11:00 L-17: UHPSFC-MS/MS versus 2D-UHPLC-MS/MS in chiral separation of Propranolol and its Hydroxy Metabolites</strong></h5>
<ul>
<li>Lukas Harps (Freie Universität Berlin, Berlin, Germany)</li>
</ul>
<h5><strong>11:00 – 11:30 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION VI: COLUMN TECHNOLOGY</strong></h4>
<ul>
<li>Chairperson: Prof. Dalibor Šatínský</li>
</ul>
<h5><strong>11:30 – 12:10 L-18: Hypercrosslinked monolithic stationary phases in proteomics</strong></h5>
<ul>
<li>Assoc. Prof. Jiří Urban (Masaryk University, Brno, Czech Republic)</li>
</ul>
<h5><strong>12:10 – 12:40 L-19: Guidelines for tuning the macropore structure of monolithic columns for high-performance liquid chromatography</strong></h5>
<ul>
<li>Dr. Sebastiaan Eeltink (Vrije Universiteit Brussel, Belgium)</li>
</ul>
<h5><strong>12:40 – 13:10 L-20: Strategies and chemistry in column development</strong></h5>
<ul>
<li>Assoc. Prof. Michal Kohout (University of Chemistry and Technology, Prague, Czech Republic)</li>
</ul>
<h5><strong>13:10 – 14:30 Lunch</strong></h5>
<h4><strong>SESSION VII: RETENTION MECHANISMS AND STATIONARY PHASES</strong></h4>
<ul>
<li>Chairperson: Prof. Spas Kolev</li>
</ul>
<h5><strong>14:30 – 15:00 L-21: Understanding the mechanism, advantages and limitations of hydrophilic interaction liquid chromatography</strong></h5>
<ul>
<li>Prof. David McCalley (University of West England, UK)</li>
</ul>
<h5><strong>15:00 – 15:30 L-22: Study of molecular structures through retention mechanisms in liquid chromatography: New trends and applications</strong></h5>
<ul>
<li>Dr. Jean-Christophe Garrigues (Paul Sabatier University - Toulouse III, Toulouse, France)</li>
</ul>
<h5><strong>15:30 – 16:00 L-23: Problems of stationary phases in SFC</strong></h5>
<ul>
<li>Prof. Lucie Nováková (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>16:00 – 16:30 L-24: The chromatographic behaviour of pharmaceuticals on stationary phases based on metal oxides</strong></h5>
<ul>
<li>Assoc. Prof. Radim Kučera (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>16:30 – 17:00 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION VIII: SAMPLE PREPARATION AND APPLICATIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Hana Sklenářová</li>
</ul>
<h5><strong>17:00 – 17:30 L-25: Glucosinolate profiling in Brassicaceae family: instrumental analysis and biological application</strong></h5>
<ul>
<li>Dr. Iva Pavlovic (Palacký University, Olomouc, Czech Republic)</li>
</ul>
<h5><strong>17:30 – 18:00 L-26: Flow programming for the development of online solid-phase extraction</strong></h5>
<ul>
<li>Assoc. Prof. Petr Chocholouš (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>18:00 – 18:30 L-27: Automated tandem homogeneous liquid-liquid extraction and centrifugation-less deproteination for complex sample analysis on HPLC</strong></h5>
<ul>
<li>Dr. Burkhard Horstkotte (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>19:00 – 22:00 Conference dinner</strong></h5>
<h3><strong>DAY 3: Wednesday 19. 10. 2022</strong></h3>
<h4><strong>WORKSHOPS: FACULTY OF PHARMACY</strong></h4>
<h5><strong>08:00 – 10:30 W1: Supercritical fluid extraction of polar and nonpolar compounds from Eucalyptus sp.</strong></h5>
<ul>
<li>Laboratory 916, 8th floor, Faculty of Pharmacy</li>
<li>Dr. Veronika Pilařová (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>08:00 – 10:30 W2: MetAmino® Kit – simple and easy sample preparation method for your LC-MC or GC-MS analysis</strong></h5>
<ul>
<li>Student’s Laboratory, 3rd floor, Faculty of Pharmacy</li>
<li>Ing. Jana Volková (Chromservis, Czech Republic)</li>
</ul>
<h5><strong>10:30 – 11:00 Coffee-break and discussion</strong></h5>
<h5><strong>11:00 – 13:30 W3: Who will help you with sample prep and liquid handling?</strong></h5>
<ul>
<li>Student’s Laboratory, 4th floor, Faculty of Pharmacy</li>
<li>Dr. Martina Riesová (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>, Prague, Czech Republic)</li>
</ul>
<h5><strong>13:30 – 14:30 Lunch</strong></h5>
<h5><strong>14:30 – 17:00 W4: Practical application of nanofibers for advanced extraction approaches in chromatography</strong></h5>
<ul>
<li>Student’s Laboratory no. 524, 4th floor, Faculty of Pharmacy</li>
<li>Prof. Dalibor Šatínský, Dr. Ivona Lhotská (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>17:00 – 18:00 Coffee-break and discussion</strong></h5>


6th STARSS Conference on Separation Science (Program)

ESAS-CSSC 2022 (Day 3)

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The Faculty of Pharmacy, with its seat in the town of Hradec Králové, was established by a government decree in 1969. This Faculty of Pharmacy has continued in the old and long-time tradition of the education of pharmacy at Charles University.

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ESAS-CSSC 2022 (Day 4)

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6th STARSS Conference on Separation Science (Program)

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