<div>In 2025, two outstanding young female researchers, Drs. Filipa Oliveira and Stella Gonsales, both originally from outside the Czech Republic, received prestigious JUNIOR STAR grants from the Czech Science Foundation (GAČR). JUNIOR STAR enables early career researchers to establish their own independent research groups and significantly advance their scientific careers. Their projects reflect cutting-edge research in materials science and sustainable chemistry, highlighting the international reach and welcoming research environment at UCT Prague.Dr. Stella Gonsales is also a&nbsp;laureate of the Dagmar Procházková Fund, which supports promising scientific projects in sustainable and advanced organic chemistry. Another recipient of this grant is Dr.&nbsp;Anna Valunchene, whose research complements UCT Prague’s&nbsp;sustainability goals. This dual funding underlines Stella’s&nbsp;remarkable achievements and the wide scope of innovative research at UCT Prague.Both Filipa and Stella are actively looking for motivated students and researchers to join their teams, offering a&nbsp;unique opportunity to work at the forefront of science in a&nbsp;vibrant and supportive environment.</div><div><h3>Interview with Filipa Oliveira: Pioneering tubular MXenes for supercapacitor energy storage</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Filipa Oliveira" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg 235w," sizes="100vw" width="1500" height="999"><h5>Congratulations on receiving the JUNIOR STAR grant. What does this achievement mean for you and how will it influence your next steps?</h5>Thank you. It’s&nbsp;a huge achievement to receive this prestigious grant. It gives me the opportunity to establish my own research group here at UCT Prague. The JUNIOR STAR program supports talented young scientists in building their teams and accessing resources. This means I&nbsp;now have the support to focus fully on my research about tubular MXenes and their application in supercapacitors.<h5>Why are tubular MXenes so innovative for energy storage?</h5>MXenes are a family of 2D materials with a layered, accordion-like structure and known for their excellent electrical conductivity. Our innovation is to produce them with a tubular morphology, combining the high conductivity and redox activity in superior ion-transport tubular architectures. Their open, hollow structure increases surface area, ion diffusion and charge transport. This could translate to faster, more efficient supercapacitors, critical for sustainable energy technologies.<h5>How did your scientific interest lead you to this field?</h5>Global challenges around green energy made me focus on materials that can improve energy storage. MXenes stood out because changes in the composition and structure can significantly change their properties. I&nbsp;was curious about how tuning different metallic elements in a&nbsp;tubular morphology can impact how MXenes perform and how to optimize them for practical applications.<h5>What challenges have you encountered?</h5>One major challenge is their stability: MXenes oxidize quickly which deteriorates performance. Another challenge is understanding how synthesis conditions of both MXenes and their precursors (MAX phases) must be adapted to create and preserve tubular morphologies since their synthesis mechanisms differ from conventional MXenes. Additionally, effectively communicating the project in the funding proposal to show to reviewers why I&nbsp;should obtain this funding was also a&nbsp;challenge, but thanks to mentorship and collaboration, I&nbsp;overcame these hurdles.<h5>What motivates you most about working at UCT Prague?</h5>The support from colleagues and the excellent technical infrastructure make it a&nbsp;great place to work. Also, being a&nbsp;former member of Professor Sofer’s&nbsp;leading group in 2D&nbsp;materials is inspiring and led me to this achievement.<h5>How do&nbsp;you see your professional development going in the future?</h5>The grant will help me build my own team, acquire equipment, and develop collaborations. My long-term vision is to contribute significantly to sustainable energy solutions and scientific progress both locally and internationally.<h5>You are looking for new team members. What kind of students or scientists do&nbsp;you need?</h5>We are currently welcoming enthusiastic master's&nbsp;students, PhD candidates, and postdocs who are passionate about materials science and eager to explore new frontiers in MXenes and energy storage. If you love curiosity-driven research and want to be part of a&nbsp;growing international team, we encourage you to join us.<h3>Interview with Stella Gonsales: Catalysis as a&nbsp;key to polymer recycling</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Stella Gonsales" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg 233w," sizes="100vw" width="1499" height="1000"><h5>Congratulations on your JUNIOR STAR grant and also being awarded the Dagmar Procházková Fund. What does receiving both mean to you and how will they shape your work?</h5>Thank you. Both grants are hugely important. The JUNIOR STAR grant allows me to expand my group and resources for research into catalytic recycling of polymers, while the Dagmar Procházková Fund supports complementary projects such as synthesizing new biodegradable polymers. Together, they help tackle the plastic waste problem from multiple scientific angles.<h5>Could you explain your research in layperson’s&nbsp;terms?</h5>Most plastics have strong carbon-carbon bonds. We insert more cleavable double bonds into polymers that can be broken down by catalytic reactions called olefin metathesis, making recycling on a&nbsp;molecular level more feasible.<h5>What makes the JUNIOR STAR grant particularly important for your research and career development?</h5>The JUNIOR STAR grant is a&nbsp;prestigious and highly competitive funding opportunity aimed at excellent early-career scientists within eight years of completing a&nbsp;Ph.D. It provides up to 25&nbsp;million CZK over five years, allowing recipients to establish independent research groups and pursue innovative scientific topics. This grant is crucial for building a&nbsp;solid foundation, acquiring necessary equipment, and recruiting talented team members, enabling the pursuit of ambitious projects that can significantly contribute to both the Czech scientific community and global challenges.<h5>What collaborations support your research?</h5>The collaboration between UCT Prague and the Institute of Organic Chemistry and Biochemistry (IOCB) of the Czech Academy of Sciences primarily provides financial and technical support. The cooperation helps with funding, access to advanced equipment, and operational resources needed for running research projects.<h5>What advice would you give young scientists aiming to succeed?</h5>I&nbsp;encourage thinking outside the box and combining diverse knowledge you acquire during your career to address complex problems that are exciting and relevant to you.<h5>Your laboratory bears the name of Otto Wichterle and was ceremonially opened in the presence of Professor Kamil Wichterle. How does Wichterle’s&nbsp;legacy inspire you personally?</h5>Wichterle’<s>’</s>s&nbsp;innovative spirit motivates me to push creative boundaries and pursue novel solutions in sustainable chemistry.<h5>What are your visions for the future?</h5>I&nbsp;aim to build a&nbsp;research group that advances sustainable polymer recycling, contributing to environmental responsibility and the circular economy.<h5>You are recruiting people for your team. What skills do&nbsp;they need to have?</h5>We are looking for talented Ph.D. students and postdocs experienced in air-sensitive catalysis techniques. If you want to contribute to transformative research in sustainable chemistry and join a&nbsp;dynamic team, this is a&nbsp;great opportunity.</div>

JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science

The clean laboratories of the CEITEC Nano research infrastructure at CEITEC Brno University of Technology house a four-meter-tall TITAN transmission electron microscope for researching materials at the atomic level. This scientific device underwent two significant upgrades this year, entailing the addition of two state-of-the-art technologies: an electromagnetic lens aberration corrector for scanning transmission electron microscopy and an electron energy loss spectrometer equipped with cameras featuring today's most sensitive sensors. Thanks to these upgrades, TITAN has become a state-of-the-art device that can only be found in a few of the world's most advanced laboratories in this configuration.The TITAN Themis microscope was manufactured in 2015 by FEI (now Thermo Fisher Scientific) and has been in use at CEITEC BUT since 2016. At the time of its acquisition, it was one of the most powerful commercially available electron microscopes; however, it lacked a corrector for optical defects in the lenses that form the narrow electron beam scanning the sample. This meant limited resolution and a lower ability to analyze the chemical composition and physical properties of materials at the atomic level."The latest generation S-CORR aberration corrector allows the electron beam to be focused to a size up to twice smaller than an atom, while also providing a higher electron current. This has led to a significant increase in the resolution and analytical capabilities of the microscope," explains Jan Michalička, guarantor of the TITAN microscope. "Thanks to the new corrector, our microscope achieves the world's highest standard in resolution, specifically less than 50 picometers at an acceleration voltage of 300 keV." For better understanding, he adds a comparison: "Imagine the ratio of the size of a golf ball to that of planet Earth. That is exactly the ratio of an atom measuring 100 pm to a golf ball." Thanks to this resolution, scientists are not only able to image individual atoms, but also to determine their chemical nature through further analysis. This is of fundamental importance, for example, for the development of new catalysts for a more environmentally friendly chemical industry.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Differences in resolution before and after the installation of the aberration corrector." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg 245w," sizes="100vw" width="1600" height="904">The new corrector also allows the microscope to achieve high resolution even with low-energy electrons. This is crucial for studying highly sensitive materials such as graphene and other two-dimensional structures, which would be damaged by a stronger electron beam. "Electrons interact strongly with the sample and can damage it at the atomic level, for example, by changing its structure and chemical composition. With the new corrector, we can achieve atomic resolution even at the lowest acceleration voltage of 60 keV, allowing us to study even very sensitive materials in a virtually unchanged state," says Jan Michalička.<h4>The additional installation of a corrector at the CEITEC laboratory is unique worldwide</h4>Correctors of optical defects in electromagnetic lenses are an exceptional technology that has completely changed the world of electron microscopy. For a long time, they were considered technically impossible, and the absence of correctors essentially prevented research into the structure and properties of materials at the atomic level. It was not until the 1990s that a group of four scientists – Harald Rose, Max Haider, Knut Urban, and Ondřej Křivánek – succeeded in implementing this technology, for which they received the prestigious Kavli Prize in 2020. “Correctors basically consist of a pair of complex lenses, each containing a series of magnetic multipoles. In the case of the correctors invented by the first three scientists mentioned above, defects are removed by two hexapole lenses. Our new corrector is based on the same concept,” adds Jan Michalička.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Delivered aberration corrector prior to installation, with the TITAN microscope in the background." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg 117w," sizes="100vw" width="1600" height="2133">The additional installation of a corrector is unique in itself nowadays. Similarly complex technologies are usually installed during the manufacture of microscopes in the factory, in this case in the Netherlands. However, in Brno, the microscope was successfully upgraded with a corrector under the limited conditions of the laboratory. The installation of the corrector took about four months and required the dismantling of a large part of the microscope. "With this installation, we basically went back to the time when the four scientists mentioned above placed their prototype corrector on an older microscope, thus improving its performance," says Jan Michalička with a smile, adding that the most difficult part was the precise adjustment of the corrector and the entire optics of the microscope in order to achieve top parameters. "Even though most things are now controlled by computers, there are only a few people in the world who can install and tune a corrector to the highest level of accuracy. We are therefore extremely pleased to have had such experts at our disposal here."<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the aberration corrector – removal of the condenser." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg 117w," sizes="100vw" width="1600" height="2133"><h4>New spectrometer will enable scientists to see more</h4>The second and equally important upgrade to the TITAN microscope is the installation of the latest generation GIF Continuum HR electron energy loss spectrometer by Gatan. This is an approximately two-meter-long device installed horizontally under the microscope, whose main component is a magnetic prism that separates the electron beam according to their energy losses. The spectra of these losses provide enormous information about the sample, principally at the atomic level, as they are characteristic of the given state of the atoms, such as the valence arrangement of their electron shell. In combination with the new corrector, the spectra can be used to analyze the chemical bonds, arrangement in the crystal lattice, or the entire range of physical properties of atomic nanostructures down to the level of individual atoms.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the spectrometer beneath the microscope, magnetic prism." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg 117w," sizes="100vw" width="1600" height="2133">An essential part of the spectrometer are the state-of-the-art K3 and Stela cameras, which can directly detect and count individual electrons. At the same time, these cameras are extremely fast and their sensors capture data without noise. "These features of our cameras are key to measuring sensitive or dynamically changing samples and allow us to obtain information that was previously invisible to electron microscopes," says Jan Michalička.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installed high-performance spectrometer cameras." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg 117w," sizes="100vw" width="1600" height="2133">Last but not least, the spectrometer will open up completely new possibilities for measuring the magnetic properties of atomic structures. The new spectrometer will also be used for such experiments within the project TERAFIT, which financed this upgrade, and which focuses on research into materials for ultra-high capacity, speed, and energy efficiency in information technology. "The device is fully prepared for advanced experiments and the use of modern software tools, including artificial intelligence," adds Vojtěch Uhlíř, head of the Nanomagnetism and Spintronics research group at CEITEC BUT and one of the TERAFIT project investigators, highlighting the advantages of the spectrometer.Together with the corrector, the spectrometer represents a breakthrough in the analysis of material properties, and according to Jan Michalička, CEITEC now has one of the most powerful microscopes available. “In modern materials science, for example, in the development of magnetic memories, quantum materials, or 2D structures, it is necessary to see not only atoms, but also their behaviour in real time, including magnetic states. And it is this spectrometer that pushes the boundaries of what we can see and analyze.” Michal Urbánek, head of the CEITEC Nano laboratories and coordinator of the large research infrastructure <a target="_blank" rel="noopener noreferrer" href="https://www.czechnanolab.cz/">CzechNanoLab</a>, agrees with him, adding that the device can now contribute to scientific knowledge on a global scale. “Thanks to its integration into the large CzechNanoLab research infrastructure, this state-of-the-art microscope is accessible not only to scientists from CEITEC, but also to other institutions in the Czech Republic and abroad.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Final configuration of the TITAN microscope after installation of the aberration corrector and spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg 117w," sizes="100vw" width="1600" height="2133"><blockquote>Do you want to know more? Read the <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/upgrade-of-titan-transmission-electron-microscope-ranks-ceitec-among-elite-laboratories-expert-version/t11696?secret=40ab0267-ec7e-11f0-8f5d-2ed9ab825d4b">expert version</a> of this article.</blockquote>

Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories

Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Article

Scientists

Science and research

Video

Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Thermo Fisher Scientific

Extracting the full potential of trace elemental analysis instrumentation to meet regulatory, compliance and performance requirements: Part 3 Triple quadrupole ICP-MS
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Presenter: Matthew Gregory (Specialist, Sales Support, TEA &amp; DIA, UK &amp; Ireland, Thermo Fisher Scientific)
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After graduating from the University of Surrey with an MChem in Chemistry, Matthew has gained 15 years’ experience of trace elemental analysis in industry, working in environmental and precious metal research laboratories. In 2018 Matthew joined Thermo Fisher Scientific as an Applications Specialist for the Trace Elemental Analysis product range in the UK and Ireland.
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Presenter: Niel Williams (Specialist, Sales Support, TEA &amp; DIA, UK &amp; Ireland, Thermo Fisher Scientific)
</blockquote>
<blockquote>
After graduating in 2014, Niel first joined Teledyne Advanced Chemistry systems as an applications specialist and later technical sales specialist. In 2019, he took the role of applications specialist for the Trace Elemental Analysis product range in the UK and Ireland at Thermo Fisher Scientific. Niel holds a BSc in Geology and Planetary science and a PhD in Isotope Geochemistry from the University of Manchester.
</blockquote>
<blockquote>
Presenter: Simon Nelms (Product Marketing Manager TEA, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Simon joined Thermo Fisher Scientific in 1996 as an applications specialist for ICP-MS, before taking a 2-year postdoctoral sabbatical at IRMM in Belgium. He re-joined the company in 2000 and in 2014 moved into Regional Marketing for the EMEA area. Simon has recently joined the Product Marketing team, covering trace elemental analysis accessories and software.
</blockquote>

Thermo Scientific: Triple quadrupole ICP-MS Educational Webinar

Triple quadrupole ICP-MS Educational Webinar

How the hardware and software of the iCAP TQe/TQ enhance performance and simplify your analysis.

The issue of spectral interferences in quadrupole ICP-MS and the negative effects these have on trace element data accuracy and detection limits has been known since the early days of the technology.
During the 1990’s, the advent of collision cell technology enabled significant reduction of a range of the most well-known interferences, such as ArO (on Fe), ArCl (on As) and ArAr (on Se) to be achieved, but challenges, such as doubly-charged ion and isobaric (same isotope mass) overlap interference, remained. The development of triple quadrupole ICP-MS in the last few years has provided a solution to these challenges by enabling much more selective interference control. With triple quadrupole instruments, this selectivity is achieved through a combination of mass pre-filtering prior to the collision cell of the instrument and use of reactive gases, such as oxygen, hydrogen and ammonia in the cell, to remove interferences via chemical reactions. In part 1 of this this 2-part webinar series, the theory and practice of triple quadrupole ICP-MS in comparison to single quadrupole ICP-MS will first be presented. The focus of the presentation will then move on to the performance of a new, workhorse instrument that utilises only He and O2 collision / reaction cell gases to remove the most commonly encountered interferences, the Thermo Scientific™ iCAP™ TQe triple quadrupole ICP-MS.
Key takeaways from the webinar:
<ul>
<li>What performance advantages triple quadrupole ICP-MS offers over single quadrupole instruments
</li>
<li>Which elements benefit from the enhanced interference removal capabilities of triple quadrupole technology
</li>
<li>What range of instruments are available for meeting the demands of both high throughput, day-to-day applications and research studies
</li>
</ul>
Who should attend:
<ul>
<li>Analysts working in the area of elemental analysis
</li>
<li>Laboratory managers looking to improve their trace element data accuracy
</li>
<li>Research academics and students who need more flexible ICP-MS technology for removing elemental interferences and reducing detection limits
</li>
</ul>
<blockquote>
Presenter: Matthew Gregory (Sales Support Specialist, Trace Elemental Analysis, Thermo Fisher Scientific)
</blockquote>
<blockquote>
After graduating from the University of Surrey with an MChem in Chemistry, Matthew has gained 15 years’ experience of trace elemental analysis in industry, working in environmental and precious metal research laboratories. In 2018 Matthew joined Thermo Fisher Scientific as an Applications Specialist for the Trace Elemental Analysis product range in the UK and Ireland.
</blockquote>

Thermo Scientific: iCAP TQe ICP-MS: For samples where single quadrupole performance isn’t enough

iCAP TQe ICP-MS: For samples where single quadrupole performance isn’t enough

Theory and practice of triple quadrupole ICP-MS in comparison to single quadrupole ICP-MS.

For samples where single quadrupole performance isn’t enough

In part 1 of this 2-part webinar series, the theory and practice of triple quadrupole ICP-MS in comparison to single quadrupole ICP-MS was presented and our new iCAP™ TQe triple quadrupole ICP-MS was introduced.
The iCAP TQe offers enhanced removal of the most commonly encountered interferences, such as argon based polyatomic species and doubly charged rare earth element interferences, using only helium and oxygen collision / reaction cell gases. However, there are some interferences (such as GdO (on Yb) and PtH (on Au)) that cannot be fully eliminated with just helium and oxygen cell gases and for these interferences the even more reactive gas, ammonia, is required. There are also some applications, such as measurement of 40Ca (which is strongly interfered by 40Ar) that benefit from the use of hydrogen cell gas. In part 2 of this webinar series, the capabilities of our fully flexible triple quadrupole ICP-MS, the Thermo Scientific™ iCAP™ TQ for removing more complex and challenging interferences will be described and results for selected applications that require use of hydrogen and ammonia cell gases will be presented.
Key takeaways from the webinar:
<ul>
<li>What performance advantages triple quadrupole ICP-MS offers over single quadrupole instruments
</li>
<li>Which elements benefit from the enhanced interference removal capabilities of triple quadrupole technology
</li>
<li>What range of instruments are available for meeting the demands of both high throughput, day-to-day applications and research studies
</li>
</ul>
Who should attend? 
<ul>
<li>Analysts working in the area of elemental analysis
</li>
<li>Laboratory managers looking to improve their trace element data accuracy
</li>
<li>Research academics and students who need more flexible ICP-MS technology for removing elemental interferences and reducing detection limits
</li>
</ul>
<blockquote>
Presenter: Niel Williams (Application Specialist, Trace Elemental Analysis, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Niel graduated from the University of Manchester with a degree in Geology and planetary science and a PhD in Isotope Cosmochemistry. Following on from this Niel worked for Teledyne Advanced Chemistry systems as an applications specialist specialising in Laser Ablation and liquid sample introduction systems. In 2019 Niel joined Thermo Fisher Scientific as an applications specialist for the Trace Elemental Analysis product range in the UK and Ireland.
</blockquote>

Thermo Scientific: iCAP TQ ICP-MS: For samples where even the iCAP TQe capabilities aren’t enough

iCAP TQ ICP-MS: For samples where even the iCAP TQe capabilities aren’t enough

Capabilities of our fully flexible triple quadrupole ICP-MS, the Thermo Scientific™ iCAP™ TQ for removing more complex and challenging interferences.

For samples where the iCAP TQe capabilities aren’t enough

<ul>
<li>Advances in triple quadrupole ICP-MS for environmental analysis
</li>
<li>Maximizing your lab productivity with true high pressure ion chromatography solutions
</li>
<li>Analysis of VOC and GRO in Water using the TriPlus 500 GC-MS Headspace Autosampler
</li>
<li>At the end of the presentations there will be time for you to ask any questions you have using the chat box
</li>
</ul>
<blockquote>
Presenter: Simon Nelms (Regional Marketing Manager, Trace Elemental, Thermo Fisher Scientific)
</blockquote>
<blockquote>
With the advent of triple quadrupole technology, a new level of interference removal in ICP-MS has been achieved. The implementation of an additional quadrupole before the collision cell of the instrument allows pre-filtering of the ion beam to selectively transmit only the masses of interest, which alleviates the problem of unwanted side interference production in the cell through collisions between other masses in the sample and the cell gas. This pre-filtering capability also allows selective chemical reactions to be conducted in the cell to enhance polyatomic interference removal and, in many cases, solve the problem of isobaric overlap. In this presentation, the latest advances in triple quadrupole ICP-MS technology and how these apply to routine analysis of environmental will be described.
</blockquote>
<blockquote>
Presenter: Seif Mohey (Technical Sales Manager Ion Chromatography, Thermo Fisher Scientific)
</blockquote>
<blockquote>
This presentation summarizes how ion chromatography has evolved over the years into today’s multimode technique suitable for solving analytical problems in all areas of interest. The Reagent-Free Ion Chromatography (RFIC) systems with electrolytic eluent generation make it possible to perform a wide range of ion chromatographic separations using only deionized water as eluent ( Just Add water Technology ). Our top of the range, Thermo Scientific™ Dionex™ ICS-6000 HPIC™ system is a modular ion chromatograph, configurable as a single or dual channel system equipped with a modern tablet interface and interactive wellness features offering superior column chemistries to maximize productivity and ease of use. This presentation brings out the advantages of using the ICS-6000 in different applications.
</blockquote>
<blockquote>
Presenter: Daniela Cavagnino (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Environmental testing laboratories involved in volatile organics determination in water and soil samples will benefit of the innovative design of theThermo Scientific™ TriPlus™ 500 HS autosampler offering scalable productivity, extended recovery and data quality, at the best cost/value. Gasoline Range Organics (GRO) are highly volatile compounds that can be easily extracted from the matrix without the need for time-consuming sample preparation. These chemicals are often present in the environment, especially in ground water and soil, mainly due to contamination incidents during handling, storing or transporting oil and related products. The TriPlus 500 HS autosampler offers a very efficient extraction of the volatiles, maximizing the sensitivity in shorter cycle times. The coupling with the GC-FID configuration provides a rugged solution for quantitative determination of volatile hydrocarbons at ppb levels, representing a robust analytical tool for routine environmental laboratories.
</blockquote>

Thermo Scientific: Innovations in Environmental Analysis

Innovations in Environmental Analysis

Advances in triple quadrupole ICP-MS for environmental analysis.

Inductively coupled plasma mass spectrometry (ICP-MS) is a key technology for trace elements in routine focused laboratories.
The determination of trace elements is regulated in a number of sample matrices, including drinking waters (EPA method 200.8 and ISO method 17294), waste waters (EPA method 6020B), foodstuffs (AOAC method 2015.01) and pharmaceutical products (United States Pharmacopeia chapters &lt;232&gt; and &lt;233&gt;).
In many cases, contract laboratories are key players to turn over the large amount of samples coming through from smaller and mid-size municipalities (unable to perform cost-efficient testing themselves), or companies that do not want to operate specialized laboratories with lower sample throughput. Contract laboratories can, therefore, offer cost-efficient analysis through higher numbers of samples, and specialize to offer dedicated solutions for key requirements. Consequently, contract laboratories have a stringent demand set on analytical instruments, allowing them to be highly cost efficient, through minimized sample turnover times, and maximized system uptime. This again is facilitated by the instrument manufacturers through intelligent solutions for sample handling, instrument performance and reduced maintenance requirements in routine operation.
New technology, such as triple quadrupole ICP-MS is key to overcoming challenges including unexpected interferences requiring post-analysis assessment of the data and labour intensive subsequent re-runs.
This on-demand webinar demonstrates how modern ICP-MS instruments, such as the Thermo ScientificTM iCAPTM RQ ICP-MS and Thermo ScientificTM iCAPTM TQ ICP-MS, can help to solve the above mentioned challenges. The presentation also shows how key metrics including turnover time per sample or maintenance required downtime can be reduced without compromising system performance.
By viewing this on-demand webinar you will...
<ul>
<li>get to know new solutions to increase sample throughput, overcome interferences more efficiently, and reduce the system downtime through care and maintenance
</li>
<li>reduce the number of re-runs, by getting quality results first time
</li>
<li>learn how easy it is using modern software tools to integrate improved technology for sampling and analysis into your laboratory and reach another level of analytical possibilities
</li>
<li>find out how your laboratory will benefit from upgrading to triple quadrupole ICP-MS routine analysis with no obstacles for your operators
</li>
</ul>
This presentation is suitable for...
<ul>
<li>operators and managers of contract labs who want to improve their ICP-MS cost of ownership through improved ICP-MS operational best practices
</li>
<li>laboratory managers of government or QA/QC laboratories who are looking for new opportunities to improve the performance of their laboratory
</li>
<li>trace-elemental analysts who want to overcome the challenges and limitations encountered with single quadrupole ICP-MS, or who are interested in how triple quadrupole technology will help them do more
</li>
<li>operators in industry and universities who want to know how this technique can facilitate everyday routine laboratory analysis
</li>
</ul>
<blockquote>
Presenter: Richard F. Jack, PhD (Thermo Fisher Scientific Inc.)
</blockquote>
<blockquote>
Richard is the senior director of vertical marketing for the environmental and industrial markets at Thermo Fisher Scientific Inc. with over 15 yrs. experience with chromatography and mass spectrometry. Richard received his PhD in Biochemistry and Anaerobic Microbiology from Virginia Tech University in Blacksburg, Virginia, USA. He has been a product manager for Hitachi High Technologies and Dionex before taking his current role.
</blockquote>
<blockquote>
Presenter: Dr Daniel Kutscher (Thermo Fisher Scientific Inc.)
</blockquote>
<blockquote>
After graduating with a degree in chemistry in 2007, Daniel obtained his PhD from the University of Oviedo in 2011. In the same year, he joined Thermo Fisher Scientific as an Application Specialist for ICP-MS, and became Product Marketing Specialist in 2018.
</blockquote>

Thermo Scientific: Accelerate Your Throughput and Be More Productive with State-of-the-Art ICP-MS Instruments

Accelerate Your Throughput and Be More Productive with State-of-the-Art ICP-MS Instruments

How modern ICP-MS instruments, such as the Thermo ScientificTM iCAPTM RQ ICP-MS and Thermo ScientificTM iCAPTM TQ ICP-MS, can help to solve challenges.

Accelerate Your Throughput and Be More Productive

Inductively coupled plasma-mass spectrometry (ICP-MS) is one of the most powerful techniques used in elemental analysis for environmental applications.
It provides the advantages of low detection limits, easy quantification, and high throughput. However, experience and best practice are necessary to best use the technique. This webcast series focuses on both routine regulatory monitoring (Part I) and solutions to improve productivity and overcome the challenges of high-matrix samples (Part II). In addition, we will discuss the use of triple quadrupole ICP-MS to meet the needs of environmental researchers and specialty environmental applications.
The webcast will cover the following:
<ul>
<li>Environmental regulations in both the U.S. and Europe
</li>
<li>Tips and tricks on EPA Methods 200.8 and 6020
</li>
<li>Autodilution for productivity improvement for high-matrix samples
</li>
<li>Advanced environmental sample analysis using triple quadrupole ICP-MS
</li>
</ul>
<blockquote>
Presenter: Marcus Manecki (Application Specialist for ICP-MS, Chromatography &amp; Mass Spectrometry Division, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Marcus graduated with a degree in Marine Chemistry at the University of Oldenburg, Germany, in 2010. Following that, Marcus joined the Organic Trace Substances Group at the Institute for Baltic Sea Research in Rostock, Germany, for three years. His main research interest at that time was the highly complex composition of dissolved organic matter and its interaction with different microbial communities. Marcus joined Thermo Fisher Scientific in 2014 as Product Specialist for ICP-MS. He is involved in finding solutions for the high throughput analysis of challenging sample types.
</blockquote>
<blockquote>
Presenter: Dhinesh Asogan (Product Specialist for ICP-MS, Chromatography &amp; Mass Spectrometry Division, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Dhinesh read Chemistry with Forensic Analysis at Loughborough University in the UK, graduating in 2005. He then joined the Centre for Innovative and Collaborative Engineering there and completed an Engineering Doctorate in laser ablation ICP-MS for semiconductor materials in 2012. From there, he joined CETAC Technologies (now Teledyne CETAC Technologies) as the European Technical Specialist for Sample Introduction Systems in Trace Elemental Spectroscopy, and then became the Laser Ablation Product Manager based in Omaha, USA. In 2016, Dhinesh joined Thermo Fisher Scientific (Bremen) GmbH as a Product Specialist in ICP-MS responsible for supporting challenging advanced applications, such as laser ablation ICP-MS.
</blockquote>

Thermo Scientific: Trace Element Analysis Using ICP-MS for Environmental Samples, Part Two: Meeting Routine Monitoring and Research Challenges

Trace Element Analysis Using ICP-MS for Environmental Samples, Part Two: Meeting Routine Monitoring and Research Challenges

Routine regulatory monitoring and solutions to improve productivity and overcome the challenges of high-matrix samples.

Trace Element Environmental Analysis Using ICP-MS

Single Quadrupole Inductively Coupled Plasma Mass Spectrometers (ICP-MS) are a long-established tool for the analysis of trace elements in a variety of sample types.
With help of a collision cell, they can overcome most interferences and determine key contaminants across many application areas with low detection limits. This is why they are commonly found in laboratories analyzing all kinds of water samples, soils, foods and feed or industrial products. Over the last years, ICP-MS systems with triple quadrupole mass analyzers (TQ-ICP-MS), enabling advanced interference removal based on reactive gases such as oxygen or ammonia, have increasingly found their way from research driven applications into applied analytical sciences, but are still not found in large number of laboratories providing service testing at a large scale. This is despite their proven track record of providing superior interference removal, improving right results first time, and increasing confidence in &quot;right first time&quot; results. The underlying reasons are mainly because of the higher initial investment not only in the instrument as such, but also in additional laboratory infrastructure, such as providing additional gases like ammonia to the lab.
Key learning objectives
<ul>
<li>Understand the differences in hardware and how they translate into better interference removal and superior detection limits
</li>
<li>See how triple quadrupole ICP-MS can help you to overcome challenges in sample type, independent of the matrix.
</li>
<li>Understand what is needed in order to leverage the full potential of a triple quadrupole ICP-MS.
</li>
<li>Learn how triple quadrupole ICP-MS can help you to improve lab efficiency and realize time savings
</li>
</ul>
<blockquote>
Presenter: Dr. Daniel Kutscher (Product Marketing Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Dr. Magda Mandic (ICP-MS Product Manager, Thermo Fisher Scientific)
</blockquote>

Thermo Scientific: Understanding the Benefits of Triple Quadrupole ICP-MS in Everyday Analysis

Understanding the Benefits of Triple Quadrupole ICP-MS in Everyday Analysis

Understand the differences in hardware and how they translate into better interference removal and superior detection limits.

Understanding the Benefits of Triple Quadrupole ICP-MS

Inorganic species, metals, gases, and organometallics play an important role in the research and analytical lab.
Learn how ICP-OES, ICP-MS, and ICP-MS/MS can help protect expensive catalysts, prevent process fouling or off-spec product, and meet environmental regulations.
<blockquote>
Presenter: Daniel Kutscher (Product Marketing Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
After graduating with a degree in Chemistry in 2007, Daniel obtained his Ph.D. from the University of Oviedo (Spain) in 2011. In the same year, he joined Thermo Fisher Scientific as an Application Specialist for ICP-MS and joined the marketing team in 2018.
</blockquote>

Thermo Scientific: It's ELEMENTary - Managing Challenges in Inorganic Analysis

It's ELEMENTary - Managing Challenges in Inorganic Analysis

Learn how ICP-OES, ICP-MS, and ICP-MS/MS can help protect expensive catalysts, prevent process fouling or off-spec product, and meet environmental regulations.

It's ELEMENTary - Managing Challenges

In the drive towards reducing dependence on burning fossil fuels for energy generation, several technologies have become established as front runners in the race to reduce carbon emissions and improve air quality.
One of the most significant is lithium ion battery technology and a rapid ramp up in production of these batteries is currently underway globally. As a result, a corresponding increase in analytical testing of the battery component materials has arisen.
Elemental analysis techniques such as ICP-OES and ICP-MS are vital for both research and quality control analysis of cathode, electrolyte and anode materials. This presentation gives a complete overview of how ICP-OES and ICP-MS analysis supports every step in the battery manufacturing value chain, from raw material screening and refining, through battery cell material research and QA/AC, to recycling and environmental analysis.
The presentation covers the following topics:
<ul>
<li>Introduction to ICP-OES and ICP-MS and what these techniques provide for battery material analysis
</li>
<li>Discussion of specific analysis examples, including bulk composition and impurity measurement of cathode active materials and electrolytes and determination of trace elements in anode materials
</li>
</ul>
By viewing this presentation, you will learn:
<ul>
<li>The basics of ICP-OES and ICP-MS technology
</li>
<li>How these technologies support battery material analysis, from raw materials to recycling
</li>
<li>What performance they achieve, demonstrated with selected analysis examples
</li>
</ul>
Who should view this presentation?
<ul>
<li>Battery material analysis laboratory managers and analysts
</li>
<li>Battery cell manufacturers and their QA/QA personnel
</li>
<li>Recycling specialists, particularly those working with battery metals such as Li, Ni, Mn, Fe and Co
</li>
<li>Scientists working in battery research and development
</li>
<li>Environmental monitoring laboratory managers
</li>
</ul>
<blockquote>
Presenter: Simon Nelms (ICP-MS Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Sukanya Sengupta (Application Specialist Trace Elemental Analysis, Thermo Fisher Scientific)
</blockquote>

Thermo Scientific: Powering Up Battery Materials Analysis using ICP-OES and ICP-MS

Powering Up Battery Materials Analysis using ICP-OES and ICP-MS

How ICP-OES and ICP-MS analysis supports every step in the battery manufacturing value chain.

ICPMS webinars focusing on ICP/MS/MS - page 5