<div>In 2025, two outstanding young female researchers, Drs. Filipa Oliveira and Stella Gonsales, both originally from outside the Czech Republic, received prestigious JUNIOR STAR grants from the Czech Science Foundation (GAČR). JUNIOR STAR enables early career researchers to establish their own independent research groups and significantly advance their scientific careers. Their projects reflect cutting-edge research in materials science and sustainable chemistry, highlighting the international reach and welcoming research environment at UCT Prague.Dr. Stella Gonsales is also a&nbsp;laureate of the Dagmar Procházková Fund, which supports promising scientific projects in sustainable and advanced organic chemistry. Another recipient of this grant is Dr.&nbsp;Anna Valunchene, whose research complements UCT Prague’s&nbsp;sustainability goals. This dual funding underlines Stella’s&nbsp;remarkable achievements and the wide scope of innovative research at UCT Prague.Both Filipa and Stella are actively looking for motivated students and researchers to join their teams, offering a&nbsp;unique opportunity to work at the forefront of science in a&nbsp;vibrant and supportive environment.</div><div><h3>Interview with Filipa Oliveira: Pioneering tubular MXenes for supercapacitor energy storage</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Filipa Oliveira" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg 235w," sizes="100vw" width="1500" height="999"><h5>Congratulations on receiving the JUNIOR STAR grant. What does this achievement mean for you and how will it influence your next steps?</h5>Thank you. It’s&nbsp;a huge achievement to receive this prestigious grant. It gives me the opportunity to establish my own research group here at UCT Prague. The JUNIOR STAR program supports talented young scientists in building their teams and accessing resources. This means I&nbsp;now have the support to focus fully on my research about tubular MXenes and their application in supercapacitors.<h5>Why are tubular MXenes so innovative for energy storage?</h5>MXenes are a family of 2D materials with a layered, accordion-like structure and known for their excellent electrical conductivity. Our innovation is to produce them with a tubular morphology, combining the high conductivity and redox activity in superior ion-transport tubular architectures. Their open, hollow structure increases surface area, ion diffusion and charge transport. This could translate to faster, more efficient supercapacitors, critical for sustainable energy technologies.<h5>How did your scientific interest lead you to this field?</h5>Global challenges around green energy made me focus on materials that can improve energy storage. MXenes stood out because changes in the composition and structure can significantly change their properties. I&nbsp;was curious about how tuning different metallic elements in a&nbsp;tubular morphology can impact how MXenes perform and how to optimize them for practical applications.<h5>What challenges have you encountered?</h5>One major challenge is their stability: MXenes oxidize quickly which deteriorates performance. Another challenge is understanding how synthesis conditions of both MXenes and their precursors (MAX phases) must be adapted to create and preserve tubular morphologies since their synthesis mechanisms differ from conventional MXenes. Additionally, effectively communicating the project in the funding proposal to show to reviewers why I&nbsp;should obtain this funding was also a&nbsp;challenge, but thanks to mentorship and collaboration, I&nbsp;overcame these hurdles.<h5>What motivates you most about working at UCT Prague?</h5>The support from colleagues and the excellent technical infrastructure make it a&nbsp;great place to work. Also, being a&nbsp;former member of Professor Sofer’s&nbsp;leading group in 2D&nbsp;materials is inspiring and led me to this achievement.<h5>How do&nbsp;you see your professional development going in the future?</h5>The grant will help me build my own team, acquire equipment, and develop collaborations. My long-term vision is to contribute significantly to sustainable energy solutions and scientific progress both locally and internationally.<h5>You are looking for new team members. What kind of students or scientists do&nbsp;you need?</h5>We are currently welcoming enthusiastic master's&nbsp;students, PhD candidates, and postdocs who are passionate about materials science and eager to explore new frontiers in MXenes and energy storage. If you love curiosity-driven research and want to be part of a&nbsp;growing international team, we encourage you to join us.<h3>Interview with Stella Gonsales: Catalysis as a&nbsp;key to polymer recycling</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Stella Gonsales" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg 233w," sizes="100vw" width="1499" height="1000"><h5>Congratulations on your JUNIOR STAR grant and also being awarded the Dagmar Procházková Fund. What does receiving both mean to you and how will they shape your work?</h5>Thank you. Both grants are hugely important. The JUNIOR STAR grant allows me to expand my group and resources for research into catalytic recycling of polymers, while the Dagmar Procházková Fund supports complementary projects such as synthesizing new biodegradable polymers. Together, they help tackle the plastic waste problem from multiple scientific angles.<h5>Could you explain your research in layperson’s&nbsp;terms?</h5>Most plastics have strong carbon-carbon bonds. We insert more cleavable double bonds into polymers that can be broken down by catalytic reactions called olefin metathesis, making recycling on a&nbsp;molecular level more feasible.<h5>What makes the JUNIOR STAR grant particularly important for your research and career development?</h5>The JUNIOR STAR grant is a&nbsp;prestigious and highly competitive funding opportunity aimed at excellent early-career scientists within eight years of completing a&nbsp;Ph.D. It provides up to 25&nbsp;million CZK over five years, allowing recipients to establish independent research groups and pursue innovative scientific topics. This grant is crucial for building a&nbsp;solid foundation, acquiring necessary equipment, and recruiting talented team members, enabling the pursuit of ambitious projects that can significantly contribute to both the Czech scientific community and global challenges.<h5>What collaborations support your research?</h5>The collaboration between UCT Prague and the Institute of Organic Chemistry and Biochemistry (IOCB) of the Czech Academy of Sciences primarily provides financial and technical support. The cooperation helps with funding, access to advanced equipment, and operational resources needed for running research projects.<h5>What advice would you give young scientists aiming to succeed?</h5>I&nbsp;encourage thinking outside the box and combining diverse knowledge you acquire during your career to address complex problems that are exciting and relevant to you.<h5>Your laboratory bears the name of Otto Wichterle and was ceremonially opened in the presence of Professor Kamil Wichterle. How does Wichterle’s&nbsp;legacy inspire you personally?</h5>Wichterle’<s>’</s>s&nbsp;innovative spirit motivates me to push creative boundaries and pursue novel solutions in sustainable chemistry.<h5>What are your visions for the future?</h5>I&nbsp;aim to build a&nbsp;research group that advances sustainable polymer recycling, contributing to environmental responsibility and the circular economy.<h5>You are recruiting people for your team. What skills do&nbsp;they need to have?</h5>We are looking for talented Ph.D. students and postdocs experienced in air-sensitive catalysis techniques. If you want to contribute to transformative research in sustainable chemistry and join a&nbsp;dynamic team, this is a&nbsp;great opportunity.</div>

JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science

The clean laboratories of the CEITEC Nano research infrastructure at CEITEC Brno University of Technology house a four-meter-tall TITAN transmission electron microscope for researching materials at the atomic level. This scientific device underwent two significant upgrades this year, entailing the addition of two state-of-the-art technologies: an electromagnetic lens aberration corrector for scanning transmission electron microscopy and an electron energy loss spectrometer equipped with cameras featuring today's most sensitive sensors. Thanks to these upgrades, TITAN has become a state-of-the-art device that can only be found in a few of the world's most advanced laboratories in this configuration.The TITAN Themis microscope was manufactured in 2015 by FEI (now Thermo Fisher Scientific) and has been in use at CEITEC BUT since 2016. At the time of its acquisition, it was one of the most powerful commercially available electron microscopes; however, it lacked a corrector for optical defects in the lenses that form the narrow electron beam scanning the sample. This meant limited resolution and a lower ability to analyze the chemical composition and physical properties of materials at the atomic level."The latest generation S-CORR aberration corrector allows the electron beam to be focused to a size up to twice smaller than an atom, while also providing a higher electron current. This has led to a significant increase in the resolution and analytical capabilities of the microscope," explains Jan Michalička, guarantor of the TITAN microscope. "Thanks to the new corrector, our microscope achieves the world's highest standard in resolution, specifically less than 50 picometers at an acceleration voltage of 300 keV." For better understanding, he adds a comparison: "Imagine the ratio of the size of a golf ball to that of planet Earth. That is exactly the ratio of an atom measuring 100 pm to a golf ball." Thanks to this resolution, scientists are not only able to image individual atoms, but also to determine their chemical nature through further analysis. This is of fundamental importance, for example, for the development of new catalysts for a more environmentally friendly chemical industry.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Differences in resolution before and after the installation of the aberration corrector." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg 245w," sizes="100vw" width="1600" height="904">The new corrector also allows the microscope to achieve high resolution even with low-energy electrons. This is crucial for studying highly sensitive materials such as graphene and other two-dimensional structures, which would be damaged by a stronger electron beam. "Electrons interact strongly with the sample and can damage it at the atomic level, for example, by changing its structure and chemical composition. With the new corrector, we can achieve atomic resolution even at the lowest acceleration voltage of 60 keV, allowing us to study even very sensitive materials in a virtually unchanged state," says Jan Michalička.<h4>The additional installation of a corrector at the CEITEC laboratory is unique worldwide</h4>Correctors of optical defects in electromagnetic lenses are an exceptional technology that has completely changed the world of electron microscopy. For a long time, they were considered technically impossible, and the absence of correctors essentially prevented research into the structure and properties of materials at the atomic level. It was not until the 1990s that a group of four scientists – Harald Rose, Max Haider, Knut Urban, and Ondřej Křivánek – succeeded in implementing this technology, for which they received the prestigious Kavli Prize in 2020. “Correctors basically consist of a pair of complex lenses, each containing a series of magnetic multipoles. In the case of the correctors invented by the first three scientists mentioned above, defects are removed by two hexapole lenses. Our new corrector is based on the same concept,” adds Jan Michalička.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Delivered aberration corrector prior to installation, with the TITAN microscope in the background." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg 117w," sizes="100vw" width="1600" height="2133">The additional installation of a corrector is unique in itself nowadays. Similarly complex technologies are usually installed during the manufacture of microscopes in the factory, in this case in the Netherlands. However, in Brno, the microscope was successfully upgraded with a corrector under the limited conditions of the laboratory. The installation of the corrector took about four months and required the dismantling of a large part of the microscope. "With this installation, we basically went back to the time when the four scientists mentioned above placed their prototype corrector on an older microscope, thus improving its performance," says Jan Michalička with a smile, adding that the most difficult part was the precise adjustment of the corrector and the entire optics of the microscope in order to achieve top parameters. "Even though most things are now controlled by computers, there are only a few people in the world who can install and tune a corrector to the highest level of accuracy. We are therefore extremely pleased to have had such experts at our disposal here."<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the aberration corrector – removal of the condenser." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg 117w," sizes="100vw" width="1600" height="2133"><h4>New spectrometer will enable scientists to see more</h4>The second and equally important upgrade to the TITAN microscope is the installation of the latest generation GIF Continuum HR electron energy loss spectrometer by Gatan. This is an approximately two-meter-long device installed horizontally under the microscope, whose main component is a magnetic prism that separates the electron beam according to their energy losses. The spectra of these losses provide enormous information about the sample, principally at the atomic level, as they are characteristic of the given state of the atoms, such as the valence arrangement of their electron shell. In combination with the new corrector, the spectra can be used to analyze the chemical bonds, arrangement in the crystal lattice, or the entire range of physical properties of atomic nanostructures down to the level of individual atoms.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the spectrometer beneath the microscope, magnetic prism." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg 117w," sizes="100vw" width="1600" height="2133">An essential part of the spectrometer are the state-of-the-art K3 and Stela cameras, which can directly detect and count individual electrons. At the same time, these cameras are extremely fast and their sensors capture data without noise. "These features of our cameras are key to measuring sensitive or dynamically changing samples and allow us to obtain information that was previously invisible to electron microscopes," says Jan Michalička.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installed high-performance spectrometer cameras." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg 117w," sizes="100vw" width="1600" height="2133">Last but not least, the spectrometer will open up completely new possibilities for measuring the magnetic properties of atomic structures. The new spectrometer will also be used for such experiments within the project TERAFIT, which financed this upgrade, and which focuses on research into materials for ultra-high capacity, speed, and energy efficiency in information technology. "The device is fully prepared for advanced experiments and the use of modern software tools, including artificial intelligence," adds Vojtěch Uhlíř, head of the Nanomagnetism and Spintronics research group at CEITEC BUT and one of the TERAFIT project investigators, highlighting the advantages of the spectrometer.Together with the corrector, the spectrometer represents a breakthrough in the analysis of material properties, and according to Jan Michalička, CEITEC now has one of the most powerful microscopes available. “In modern materials science, for example, in the development of magnetic memories, quantum materials, or 2D structures, it is necessary to see not only atoms, but also their behaviour in real time, including magnetic states. And it is this spectrometer that pushes the boundaries of what we can see and analyze.” Michal Urbánek, head of the CEITEC Nano laboratories and coordinator of the large research infrastructure <a target="_blank" rel="noopener noreferrer" href="https://www.czechnanolab.cz/">CzechNanoLab</a>, agrees with him, adding that the device can now contribute to scientific knowledge on a global scale. “Thanks to its integration into the large CzechNanoLab research infrastructure, this state-of-the-art microscope is accessible not only to scientists from CEITEC, but also to other institutions in the Czech Republic and abroad.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Final configuration of the TITAN microscope after installation of the aberration corrector and spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg 117w," sizes="100vw" width="1600" height="2133"><blockquote>Do you want to know more? Read the <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/upgrade-of-titan-transmission-electron-microscope-ranks-ceitec-among-elite-laboratories-expert-version/t11696?secret=40ab0267-ec7e-11f0-8f5d-2ed9ab825d4b">expert version</a> of this article.</blockquote>

Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories

Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Article

Scientists

Science and research

Video

Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Agilent Technologies

Thermo Fisher Scientific

Shimadzu Corporation

Even if you have robust quality systems governing your analytical laboratory and a strong data integrity program in place, you may still be nervous about preparing for a regulatory inspection. What will the inspectors want to know? What will they ask? Is your team prepared?
You can ease those fears by attending this two-part webcast. First, we will explain what you should expect during an inspection. Then, we will simulate an inspection to show you, in a realistic scenario, what an inspector might ask, and how you and your team should handle the questions, including when they don’t know all the answers or don’t understand all the questions. We will explain the scope of a data integrity inspection in a regulated laboratory, and our simulation of an inspection of a chromatography data system (CDS) will explain the roles of different people in a multi-person team that includes a laboratory manager and the CDS laboratory administrator.
<h3>What we will cover:</h3>
<ul>
<li>Set up of a chromatography data system (CDS) and sample analysis</li>
<li>Peak integration</li>
<li>Review and e-signatures</li>
<li>Investigations of out-of-specification (OOS) results and invalidation of OOS results</li>
</ul>
<h3>Key Learning Objectives:</h3>
<ul>
<li>Understand how data integrity plays a role in your inspection</li>
<li>Understand and answer the questions being asked</li>
<li>Understand the impact of poor analytical decisions</li>
<li>Use systems that can help demonstrate data integrity and regulatory compliance</li>
<li>Use technical controls to enforce operating procedures</li>
</ul>
<h3>Who Should Attend:</h3>
<ul>
<li>Analysts working in a regulated analytical laboratory</li>
<li>Chromatography data system (CDS) administrators</li>
<li>Laboratory managers</li>
<li>Quality assurance (QA) and quality control (QC) staff</li>
</ul>
<blockquote>
Presenter: Bob McDowall (Director, RD McDowall Limited, Bromley, Kent, UK)
</blockquote>
<blockquote>
Bob McDowall, PhD, is an analytical chemist with over 45 years of experience including 15 years working in the pharmaceutical industry and over 25 years working for the industry as a consultant. He is the director of R D McDowall Ltd, a consulting company offering services on process improvement, implementation of laboratory informatics applications, training staff in regulatory compliance, and qualification of IT infrastructure. Bob is a trained auditor and has been involved with the validation of computerized systems for over 30 years. He edited the first book on LIMS in 1987 and is the author of books on the validation of chromatography data systems and data integrity and data governance in regulated laboratories due to be published in late 2018. He was presented with the 1997 LIMS Award. He is a core industry expert for the GAMP Data Integrity Special Interest Group and was a subject matter expert contributor and reviewer of the 2017 GAMP Guide for Records and Data Integrity. He co-drafted the update to USP &lt;1058&gt; on Analytical Instrument Qualification (AIQ) for public comment that became effective in August 2017.
</blockquote>
<blockquote>
Presenter: Paul Smith (Global Compliance Specialist, Agilent Technologies, Cheadle, UK)
</blockquote>
<blockquote>
Paul is global compliance specialist with Agilent technologies. He has worked for Agilent for 9 years and has 35 years’ experience in the pharmaceutical and analytical area.
</blockquote>
<blockquote>
Presenter: Josh Miller (OpenLab Software Marketing – eLearning Expert, Agilent Technologies, Santa Clara, CA)
</blockquote>
<blockquote>
Josh is a professional chemist with 15 years’ experience in validating, supporting, deploying, and developing the OpenLab software suite of products. He is a true expert with all things OpenLab and has extensive experience with chromatography data system, electronic content management, business process management, instrument control products, and more. He has developed many of the training courses for OpenLab products and has served as an instructor for dozens of formal courses around the globe. In 2009, he created “eFamiliarization” to help customers learn the basics of OpenLab products in an interactive simulation of Agilent software. More recently he has transformed Agilent’s embedded software help systems to better serve customers by putting consistent and searchable content at their fingertips. You may also find a significant collection of video content that he produces to help users learn to use OpenLab products.
</blockquote>

LCGC/Agilent Technologies: Five Essentials for Surviving Your Next Laboratory Inspection: A Quality Control Example, Part 2

Five Essentials for Surviving Your Next Laboratory Inspection: A Quality Control Example, Part 2

Learn how to transform your unknown samples into routine analyses. Utilizing ICP-Expert’s Smart features, any analyst can easily develop a method that offers greater sample insight than ever before – leading to better results.
Join us to see how you can become a more savvy ICP-OES analyst.
<blockquote>
Presenter: Ana García-González (Atomic Spectroscopy Application Scientist, Agilent Technologies)
</blockquote>
<blockquote>
Ana Garcia is the Atomic Spectroscopy Application Scientist at Agilent Technologies. Ana has a broad background in atomic spectroscopy technologies, including international experience developing and validating analytical spectroscopy methods for the geochemical industry. She has also published in the fields of molecular spectroscopy and chemometrics. Ana holds a MSc in plant Biotechnology at CINVESTAV, México, and a Bachelor of Science in Biotechnology Engineering.
</blockquote>
<blockquote>
Presenter: Chris Conklin (Atomic Spectroscopy Product Specialist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
With a degree from the University of Wisconsin – Eau Claire, Chris worked in, and lead, a quality control lab testing fine chemicals ranging from reagent grade to high purity. Over the course of 12 years in that role, Chris has run a variety of atomic elemental instruments and techniques including AA, ICP-OES, and ICP-MS. As a result, he has seen most of the periodic table in its elemental form and overcome the associated interferences. In 2018, Chris brought that knowledge and experience to his current role with Agilent as the Product Specialist for Atomic Spectroscopy supporting AA, MP-AES, and ICP-OES for the Eastern US.
</blockquote>

Agilent Technologies: Tips and Tricks Workshop on ICP-OES: Smarter Method Development

Tips and Tricks Workshop on ICP-OES: Smarter Method Development

Learn how to transform your unknown samples into routine analyses.

Tips and Tricks Workshop on ICP-OES: Method Development

How to avoid potential non-compliance with regulatory guidelines in challenging times.
The complexities of maintaining compliance and audit-readiness in regulated environments take significant time and resources. Learn in this part of the session how to mitigate the risk of potential non-compliances as you return to the lab or transition instrumentation to meet new demands with operational qualification and requalification services from Unity Lab Services. See how you can save time and improve your laboratory’s performance. 
<h3>Learn about:</h3>
<ul>
<li>Qualification Services for Analytical Instruments and Laboratory Equipment </li>
<li>Range of qualification capabilities that provide improved productivity, single-vendor convenience, a no-charge requalification guarantee</li>
</ul>
<blockquote>
Presenter: Martin Wheeler (Regulatory Product Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Martin has worked since 1997 within the organization focusing on single quadrupole instrumentation using Gas &amp; Liquid Chromatography together with Mass Spectrometers, CDS and Layered Applications. His current role as Regulatory Product Manager includes compliance and qualification procedures working closely as part of the Regulatory Product Support Group assisting Field based Engineers, as well as creating/updating qualification protocols.
</blockquote>
<blockquote>
Presenter: Julian Renpenning - Moderator (Regional Marketing Manager EMEA, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Julian studied at the Technical University of Berlin Biotechnology and specialized in analytical chemistry and bioprocess engineering. In 2011 he started his PhD at the Environmental Research in Leipzig, Germany and continued in 2015 to work as Postdoc career on analytical method development for hyphenated MS, IRMS and ICP-MS with gas chromatography. Since 2019 I took a role at TFS as EMEA Regional Marketing Manager for GC, GC-MS and IOMS.
</blockquote>

Thermo Fisher Scientific - Webinars Live and on-demand

Moving forward webinar part 3 - compliance

Inductively Coupled Plasma Mass Spectrometry is the industry standard for elemental impurities analysis in pharmaceuticals, due to very low detection limits and the robust nature of this technology.
While the instrumentation is extremely accurate, this analytical technique is prone to errors from sample preparation. These include contamination from the environment and equipment, reagents, digestion, and various sample handling techniques. In this webinar we will discuss tips and tricks for reducing contamination and ensuring the most accurate results possible.

Shimadzu: Elemental Impurities in Pharmaceuticals – Sample Preparation Methods and Tips for ICP-MS

Elemental Impurities in Pharmaceuticals – Sample Preparation Methods and Tips for ICP-MS

Tips and tricks for reducing contamination and ensuring the most accurate results possible.

Elemental Impurities in Pharmaceuticals by ICP-MS

Our experts will discuss ICP-MS tips and tricks and innovations that resolve the most complex interferences, streamline method development, and simplify analytical workflows.
<blockquote>
Presednter: Daniel Kutscher (Product Marketing Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Daniel graduated with his degree in Chemistry from the University of Mainz, Germany. He then joined the Analytical Spectrometry Group at the University of Oviedo, Spain, where he obtained his Ph.D. His main research interest in that time was the use of elemental labeling of proteins and peptides to explore new technologies based on elemental mass spectrometry for their accurate quantification.
</blockquote>
<blockquote>
Daniel joined Thermo Fisher Scientific in 2011 as a Product Specialist for ICP-MS. Besides working with hyphenated techniques such as IC-ICP-MS, he is also involved in finding solutions for the high-throughput analysis of challenging sample types.
</blockquote>

Thermo Scientific: Champion Your ICP-MS Analysis: Tips, Tricks and Innovations for Routine and Advanced Applications

Champion Your ICP-MS Analysis: Tips, Tricks and Innovations for Routine and Advanced Applications

ICP-MS tips and tricks and innovations that resolve the most complex interferences, streamline method development, and simplify analytical workflows.

Champion Your ICP-MS Analysis: Tips, Tricks and Innovations

Inductively coupled plasma mass spectrometry (ICP-MS) is a key technology for trace elements in routine focused laboratories.
The determination of trace elements is regulated in a number of sample matrices, including drinking waters (EPA method 200.8 and ISO method 17294), waste waters (EPA method 6020B), foodstuffs (AOAC method 2015.01) and pharmaceutical products (United States Pharmacopeia chapters &lt;232&gt; and &lt;233&gt;).
In many cases, contract laboratories are key players to turn over the large amount of samples coming through from smaller and mid-size municipalities (unable to perform cost-efficient testing themselves), or companies that do not want to operate specialized laboratories with lower sample throughput. Contract laboratories can, therefore, offer cost-efficient analysis through higher numbers of samples, and specialize to offer dedicated solutions for key requirements. Consequently, contract laboratories have a stringent demand set on analytical instruments, allowing them to be highly cost efficient, through minimized sample turnover times, and maximized system uptime. This again is facilitated by the instrument manufacturers through intelligent solutions for sample handling, instrument performance and reduced maintenance requirements in routine operation.
New technology, such as triple quadrupole ICP-MS is key to overcoming challenges including unexpected interferences requiring post-analysis assessment of the data and labour intensive subsequent re-runs.
This on-demand webinar demonstrates how modern ICP-MS instruments, such as the Thermo ScientificTM iCAPTM RQ ICP-MS and Thermo ScientificTM iCAPTM TQ ICP-MS, can help to solve the above mentioned challenges. The presentation also shows how key metrics including turnover time per sample or maintenance required downtime can be reduced without compromising system performance.
By viewing this on-demand webinar you will...
<ul>
<li>get to know new solutions to increase sample throughput, overcome interferences more efficiently, and reduce the system downtime through care and maintenance
</li>
<li>reduce the number of re-runs, by getting quality results first time
</li>
<li>learn how easy it is using modern software tools to integrate improved technology for sampling and analysis into your laboratory and reach another level of analytical possibilities
</li>
<li>find out how your laboratory will benefit from upgrading to triple quadrupole ICP-MS routine analysis with no obstacles for your operators
</li>
</ul>
This presentation is suitable for...
<ul>
<li>operators and managers of contract labs who want to improve their ICP-MS cost of ownership through improved ICP-MS operational best practices
</li>
<li>laboratory managers of government or QA/QC laboratories who are looking for new opportunities to improve the performance of their laboratory
</li>
<li>trace-elemental analysts who want to overcome the challenges and limitations encountered with single quadrupole ICP-MS, or who are interested in how triple quadrupole technology will help them do more
</li>
<li>operators in industry and universities who want to know how this technique can facilitate everyday routine laboratory analysis
</li>
</ul>
<blockquote>
Presenter: Richard F. Jack, PhD (Thermo Fisher Scientific Inc.)
</blockquote>
<blockquote>
Richard is the senior director of vertical marketing for the environmental and industrial markets at Thermo Fisher Scientific Inc. with over 15 yrs. experience with chromatography and mass spectrometry. Richard received his PhD in Biochemistry and Anaerobic Microbiology from Virginia Tech University in Blacksburg, Virginia, USA. He has been a product manager for Hitachi High Technologies and Dionex before taking his current role.
</blockquote>
<blockquote>
Presenter: Dr Daniel Kutscher (Thermo Fisher Scientific Inc.)
</blockquote>
<blockquote>
After graduating with a degree in chemistry in 2007, Daniel obtained his PhD from the University of Oviedo in 2011. In the same year, he joined Thermo Fisher Scientific as an Application Specialist for ICP-MS, and became Product Marketing Specialist in 2018.
</blockquote>

Thermo Scientific: Accelerate Your Throughput and Be More Productive with State-of-the-Art ICP-MS Instruments

Accelerate Your Throughput and Be More Productive with State-of-the-Art ICP-MS Instruments

How modern ICP-MS instruments, such as the Thermo ScientificTM iCAPTM RQ ICP-MS and Thermo ScientificTM iCAPTM TQ ICP-MS, can help to solve challenges.

ICPMS webinars focusing on Tips & Tricks - page 12