<p><strong>The goal of this study is to advance molecular epidemiology by integrating and analyzing large-scale population data from two complementary platforms: quantitative NMR spectroscopy for 209 lipoprotein measures and LC-MS/MS for 809 lipid classes and species. Together, these datasets encompass 1018 molecular measures across 7830 participants from two population cohorts. The study aims to elucidate the molecular and compositional relationships within the circulatory lipid transport system and their connections to physiological and clinical characteristics.</strong></p><p>Through cluster analyses and unsupervised neural network techniques (self-organizing maps), the study identifies inherent links between deep molecular data and population health traits, including cardiometabolic risk factors such as lipoprotein lipids, apolipoprotein B, ceramides, and oxidized lipids. Notable findings include the consistent cardiometabolic risk associations of triglyceride species across the lipoprotein cascade and the intricate metabolic profiles of cholesteryl ester species, which vary in their risk implications. This work highlights the unprecedented detail and compatibility of combined omics methodologies, paving the way for comprehensive molecular insights and new opportunities in large-scale population health studies.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229"><strong>Personalized Profiling of Lipoprotein and Lipid Metabolism Based on 1018 Measures from Combined Quantitative NMR and LC-MS/MS Platforms</strong></a></h4><p>Siyu Zhao, Corey Giles, Kevin Huynh, Johannes Kettunen, Marjo-Riitta Järvelin, Mika Kähönen, Jorma Viikari, Terho Lehtimäki, Olli T. Raitakari, Peter J. Meikle, Ville-Petteri Mäkinen, Mika Ala-Korpela</p><p>Analytical Chemistry, Vol 96/Issue 52</p><p><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229">https://doi.org/10.1021/acs.analchem.4c03229</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p>&nbsp;</p><p class="cke-user_style-important"><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><h3><strong>Abstract</strong></h3><p>Applications of advanced omics methodologies are increasingly popular in biomedicine. However, large-scale studies aiming at clinical translation are typically siloed to single technologies. Here, we present the first comprehensive large-scale population data combining 209 lipoprotein measures from a quantitative NMR spectroscopy platform and 809 lipid classes and species from a quantitative LC-MS/MS platform. These data with 1018 molecular measures were analyzed in two population cohorts totaling 7830 participants. The association and cluster analyses revealed excellent coherence between the methodologically independent data domains and confirmed their quantitative compatibility and suitability for large-scale studies. The analyses elucidated the detailed molecular characteristics of the heterogeneous circulatory macromolecular lipid transport system and the underlying structural and compositional relationships. Unsupervised neural network analysis─the so-called self-organizing maps (SOMs)─revealed that these deep molecular and metabolic data are inherently related to key physiological and clinical population characteristics. The data-driven population subgroups uncovered marked differences in the population distribution of multiple cardiometabolic risk factors. These include, e.g., multiple lipoprotein lipids, apolipoprotein B, ceramides, and oxidized lipids. All 79 structurally unique triglyceride species showed similar associations over the entire lipoprotein cascade and indicated systematically increased risk for carotid intima media thickening and other atherosclerosis risk factors, including obesity and inflammation. The metabolic attributes for 27 individual cholesteryl ester species, which formed six distinct clusters, were more intricate with associations both with higher─e.g., CE(16:1)─and lower─e.g., CE(20:4)─cardiometabolic risk. The molecular details provided by these combined data are unprecedented for molecular epidemiology and demonstrate a new potential avenue for population studies.</p><p>Lipoprotein lipids have had a central role in clinical risk assessment for cardiometabolic diseases since the 1950s. <a href="javascript:void(0);" aria-label="reference 1">(1)</a> The realization that <sup>1</sup>H NMR spectroscopy would be inherently suited for lipoprotein analytics took place in the early 1990s. <a href="javascript:void(0);" aria-label="reference 2">(2)</a> In addition to the fundamental advantages of <sup>1</sup>H NMR spectroscopy in producing quantitative data, the spherical monolayered structure of lipoprotein particles provides a compelling attribute that the chemical shifts of lipid molecules transported by these particles are dependent on the particle diameter. This phenomenon arises from the orientational order of the surface lipids and the resulting anisotropy of the magnetic susceptibility, and leads to distinctive particle size-dependent frequency shifts. This facilitates comprehensive lipoprotein analytics that is pertinent to the metabolic constituents of the lipoprotein cascade. Practical applications and statistical considerations have indicated that 14 lipoprotein subclasses and their main lipid constituents can be robustly quantified. <a href="javascript:void(0);" aria-label="reference 3 and 4">(3,4)</a> Multiple commercial methodologies have also been developed. <a href="javascript:void(0);" aria-label="reference 5 to 7">(5−7)</a> Here we applied a platform that has been widely applied in large-scale epidemiology and genetics over the last 15 years with some 500 publications and 2 M samples analyzed, including all the 0.5 M serum samples in the UK Biobank. <a href="javascript:void(0);" aria-label="reference 3, 5 and 8">(3,5,8)</a> This particular platform has also played a key role in yielding open access genetic data, with the most recent genome-wide association study meta-analysis being carried out in 136,000 individuals. <a href="javascript:void(0);" aria-label="reference 9">(9)</a></p><p>Mass spectrometry based lipidomics methodologies have been developed independent of the above and are technologically more challenging than the applications of <sup>1</sup>H NMR spectroscopy. <a href="javascript:void(0);" aria-label="reference 10 to 12">(10−12)</a> Nevertheless, epidemiological applications have become feasible over the recent years and clinical translation has been suggested, for example, with respect to assessing cardiometabolic risk <a href="javascript:void(0);" aria-label="reference 13 and 14">(13,14)</a> and cognitive impairment. <a href="javascript:void(0);" aria-label="reference 15">(15)</a> Initial genetic studies have also been published. <a href="javascript:void(0);" aria-label="reference 16 and 17">(16,17)</a></p><p>The NMR spectroscopy and MS lipidomics approaches to lipid characterization and quantification are fundamentally different (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>). In NMR the serum sample is directly analyzed with the lipoprotein particles intact; this allows quantification of the comprehensive and specific data on the lipoprotein subclasses and lipids. <a href="javascript:void(0);" aria-label="reference 18">(18)</a> Unlike NMR, LC-MS/MS lipidomics calls for lipid extraction of the serum samples as an integral prerequisite for the analyses. <a href="javascript:void(0);" aria-label="reference 19">(19)</a> The lipidomics sample preparations thus represent pooled mixtures of all the lipid molecules from all the circulating lipoprotein particles. All the information from the specific lipoprotein origin of a certain lipid in the circulation is lost in the extraction phase. However, with LC-MS/MS hundreds of unique lipid species can be analyzed from dozens of different molecular lipid classes. <a href="javascript:void(0);" aria-label="reference 12 and 20">(12,20)</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2024_96_52_20362_20370_Figure_1_Schematic_illustration_of_the_methodological_differences_and_analytical_characteristics_between_1_H_NMR_and_LC_MSMS_lipidomics_and_their_molecular_views_into_serum_b9907a5e30.jpeg" alt="Anal. Chem. 2024, 96, 52, 20362-20370: Figure 1. Schematic illustration of the methodological differences and analytical characteristics between 1H NMR and LC-MS/MS lipidomics and their molecular views into serum lipids. (A) The lipoprotein analysis with NMR is performed directly from the intact serum sample. (B) For the LC-MS/MS analysis all the lipids need to be extracted to a homogeneous lipid-soluble mixture. This allows LC-MS/MS to reach detailed molecular quantification, however, as a total serum concentration without any information on the originating lipoproteins. (C,D) The NMR analysis gives molecular information on 14 lipoprotein subclasses with the abundant core and surface lipids quantified. The percentages depict the compositional variation of these lipids in different lipoprotein particles." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2024_96_52_20362_20370_Figure_1_Schematic_illustration_of_the_methodological_differences_and_analytical_characteristics_between_1_H_NMR_and_LC_MSMS_lipidomics_and_their_molecular_views_into_serum_b9907a5e30.jpeg 192w," sizes="100vw" width="947" height="771"></p><p>Lipoprotein particles are the sole transport vehicles for all lipid molecules in serum, <a href="javascript:void(0);" aria-label="reference 18, 21 and 22">(18,21,22)</a> though albumin contains the bulk of free fatty acids and acylcarnitines, and transports also various lyso-type of lipid molecules. <a href="javascript:void(0);" aria-label="reference 23 and 24">(23,24)</a> The NMR and the LC-MS/MS data on serum essentially represent the same lipids as noted by Ghorasaini et al. <a href="javascript:void(0);" aria-label="reference 25">(25)</a> The NMR provides the size-resolution and metabolic information on lipoprotein particles (209 measures) and the LC-MS/MS lipidomics opens up a prolific and detailed molecular view on all the circulating lipids (809 measures).</p><p>These different methodologies and molecular views to study serum lipids have independently advanced toward large-scale population studies. Here, we integrate these state-of-the-art data─over 1000 quantitative molecular lipid measures per individual─in two large-scale population cohorts of over 7800 participants. These data reveal the fundamental relationships between the lipoprotein subclass cascade and metabolism (NMR) and the plethora of specific molecular information (LC-MS/MS). The molecular and metabolic corollaries are numerous, and we elaborate on some pertinent findings. Among the various analyses we also demonstrate the admirable coherence within the independent experiments and data domains. It is also uncovered─via data-driven, unsupervised neural network analysis─that these molecular lipid measures per se can be utilized for metabolically and clinically meaningful subgrouping of populations. The results illustrate that these combined comprehensive data lead to improved molecular characterization of population-level metabolic health and cardiometabolic risk.</p><h3><strong>Materials and Methods</strong></h3><h5><strong><sup>1</sup>H NMR Spectroscopy</strong></h5><p>We applied an NMR platform that has been widely used in epidemiology and for which the general methodological issues have been published. <a href="javascript:void(0);" aria-label="reference 3, 5, 8, 9 and 18">(3,5,8,9,18)</a> The separation of lipoprotein subclasses by proton NMR spectroscopy is based on particle size, <a href="javascript:void(0);" aria-label="reference 2">(2)</a> the platform resolution being 14 subclasses (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229#fig2" data-tab="pane-pcw-Figures" id="">Figure 2</a>). <a href="javascript:void(0);" aria-label="reference 3 and 18">(3,18)</a> Independent verification for the number of subclasses has been published by Mihaleva and co-workers with an in-depth handling of statistical grounds. <a href="javascript:void(0);" aria-label="reference 4">(4)</a> Particle (P), phospholipid (PL), free cholesterol (FC), cholesteryl ester (CE), and triglyceride (TG) concentrations are quantified for all 14 subclasses, also allowing calculation of the relative lipid composition for each lipoprotein subclass. The platform also provides all key clinically used lipid measures (e.g., total TG, cholesterol, and HDL-C) as well as apolipoprotein B (apoB) and A-I (apoA-I) concentrations. In total 209 lipoprotein-related measures were analyzed (98 concentration and 70 compositional measures from 14 lipoprotein subclasses, 19 clinical lipid measures, and 22 measures related to fatty acids, average particle sizes, and apoA-I and apoB).</p><h5><strong>LC-MS/MS Mass Spectrometry</strong></h5><p>Serum lipids were extracted as previously described. <a href="javascript:void(0);" aria-label="reference 19">(19)</a> The LC-MS/MS profiling was performed using the standardized methodology described recently by Huynh et al. <a href="javascript:void(0);" aria-label="reference 12">(12)</a> and updated with a larger set of targets. The mass spectrometric details and parameters are given in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c03229/suppl_file/ac4c03229_si_001.pdf">Supporting Information</a>.</p><h3><strong>Conclusions</strong></h3><p>The presented combination of lipoprotein and lipid data from NMR spectroscopy and LC-MS/MS lipidomics led to an unprecedented metabolic and molecular detail with over 1000 measures per person in a population setting of over 7800 individuals. The analyses demonstrated notable coherence between the data from inherently different spectroscopic techniques. The data-driven analyses revealed multiple confirmatory and novel molecular results in relation to systemic lipid metabolism and illustrated the power of all-inclusive lipid data in understanding cardiometabolic population health, related clinical factors, and disease risk. The analyses emphasized the augmented value of interpreting lipidomics data (deep molecular phenotyping) together with lipoprotein data (deep metabolic phenotyping) and association heatmaps between all the lipidomics measures and the key measures characterizing lipoprotein subclass metabolism are provided. These unique motifs are useful as guidelines for the key lipoprotein mediators for various lipidomics associations in biomedical studies when comprehensive lipoprotein profiles are not available.</p>

Personalized Profiling of Lipoprotein and Lipid Metabolism Based on 1018 Measures from Combined Quantitative NMR and LC-MS/MS Platforms

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 20th January 2025? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Shimadzu, Agilent Technologies </strong>and<strong> Thermo Fisher Scientific!</strong></p><h4><strong>1. Shimadzu: TOC Analysis for Evaluation of Plastics and Other Organic Materials in Contact with Drinking Water</strong></h4><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/paper/28646"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>TOC analysis helps ensure compliance with EU regulations for materials in contact with drinking water, protecting public health and maintaining high water quality standards.</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?instrumentationItems=103&amp;mainauthorItems=1&amp;search=TOC-L+CPH"><strong>TOC-L </strong></a>supports a comprehensive analysis capable of testing a wide range of components, thus helping to obtain conformity certification for the hygienic suitability of products in contact with drinking water.</li></ul><h5><strong>Introduction</strong></h5><p>Ensuring the purity of drinking water during production and distribution is a critical requirement. Quality standards are defined by the European Union in the Drinking Water Directive (EU) 2020/2184, where Article 11 specifically addresses materials that come into contact with water (Table 1) . These materials must not adversely affect human health, alter the taste, color or odor of the water, contribute to microbial growth or release significant quantities of contaminants. In essence, no extractable substances from these materials should contaminate drinking water. Therefore, the technical and hygienic suitability of products in contact with drinking water must be verified by laboratory tests.</p><p>Article 11 (EU) of 2020/2184 is essentially based on the "4 Member States Initiative" (4MSI) common approach for organic materials. One guideline that has dealt with this topic to date is the KTW guideline (German for Plastics - Drinking Water) from the German Federal Environment Agency. It was used for the hygienic assessment of organic materials in contact with<br>drinking water and describes the requirements that plastics must meet in this context. As guidelines tend to be of a recommendatory nature, the EU has obliged its member states to specifically regulate the requirements for materials in contact with drinking water. Accordingly, the Federal Environment Agency published the KTW-BWGL (Plastics Drinking Water Assessment Guideline) in March 2019. It officially replaced the previous recommendations of the KTW guideline.</p><h4><strong>2.&nbsp;Agilent Technologies: Agilent 8800 ICP-QQQ Upgrades</strong></h4><ul><li>Other</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/paper/28650"><strong>Full PDF for download</strong></a></li></ul><p>Upgrading your 8800 to an Agilent 8900 provides a seamless transition, as the new instrument can replace your existing 8800 with minimal disruption to your lab’s operations:</p><ul><li>Existing laboratory services – power, exhaust vent, and gas supplies – will still be suitable</li><li>Major peripherals such as the ASX and SPS 4 autosamplers and water chiller/recirculator can still be used</li><li>Many consumables (torches, sampling cones, tubing) are compatible</li><li>Upgrading also gives you access to the latest in ICP-QQQ technology as well as the upgraded UHMI High Matrix Introduction system<sup>1</sup>.</li></ul><h5><strong>Upgrading your Agilent 8800 ICP-QQQ</strong></h5><p>For a limited time, Agilent is offering 8800 Series ICP-QQQ users the opportunity to upgrade their ICP-QQQ instrumentation to the latest, high-performance, Agilent triple quadrupole ICP-QQQ. The Agilent 8900 is the ideal replacement for your 8800, as it provides continuity of analytical capability but with even better performance. The 8900 is also compatible with your existing bench space and lab services.</p><p>With the 8900, there is no need to compromise the unmatched matrix tolerance, helium, and unparalleled reaction cell mode performance and wide detector dynamic range that made the 8800 such a remarkable success.</p><h4><strong>3.&nbsp;Thermo Fisher Scientific: A comprehensive workflow for beverage analysis using inductively coupled plasma mass spectrometry (ICP-MS)</strong></h4><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/paper/28429"><strong>Full PDF for download</strong></a></li></ul><p>This application note will highlight a complete and comprehensive workflow for the analysis of nutrients and toxic trace elements in different types of beverages. Samples were digested using a microwave system prior to analysis using triple quadrupole ICP-MS for analysis.</p><h5><strong>Introduction</strong></h5><p>Trace metals analysis in beverages plays a crucial role in ensuring the safety, quality, and compliance of these products, helping to protect consumer health and maintain the reputation of beverage manufacturers.</p><p>Trace metals can enter beverages through various sources, starting from their natural occurrence in the ingredients, water used in production, or through processing equipment, packaging materials, etc. The presence of these metals in excessive amounts can pose potential health risks to consumers, making it necessary to monitor and control their levels. Typical analytes include, but are not limited to, arsenic, cadmium, lead, mercury, and copper. The analysis is essential to ensure the safety and quality of beverages consumed by humans.</p><p>Regulatory bodies, such as the World Health Organization (WHO) and the Food and Drug Administration (FDA), have established guidelines and maximum allowable limits for trace metals in beverages to ensure consumer safety. Manufacturers and regulatory authorities rely on the analysis to verify compliance with these regulations and to maintain the quality and integrity of beverages in the market.</p><p>It is important to note that concentration limits can vary depending on the specific beverage type and the intended consumer population (e.g., infants, children, adults). Furthermore, applicable regulations and guidelines are regularly updated, so it is crucial for beverage manufacturers to stay informed about the latest requirements in their respective markets to ensure compliance.</p><p>On the other hand, there are several essential inorganic elements that are vital for various physiological processes in the body. Nutrients such as calcium, phosphorous, sodium, potassium, magnesium, iron, and zinc serve essential roles in various physiological processes within the human body and provide the building blocks for growth, development, and maintenance of tissues and organs. These elements therefore play crucial roles in maintaining overall health and supporting various bodily functions.</p><p>This application note demonstrates a workflow for highly reproducible sample preparation combined with sensitive and robust analysis for a wide variety of beverages. The proposed workflow allowed accurate determination of both essential nutrients and potentially toxic trace metals without any compromise and can be implemented, for example, in analytical testing laboratories performing the analysis on behalf of manufacturers or regulatory authorities.</p><h5><strong>Conclusion</strong></h5><p>This workflow, combining sample preparation using microwave assisted digestion and analysis using the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1488"><strong>iCAP MTX ICP-MS</strong></a>, allows the determination of nutrient composition of fruit juices and processed beverages as required by the food labeling regulations and detection of any contaminants such as toxic elements present in the beverages.</p><p>The analytical method was proven to provide accurate and precise results and can provide reliable performance over long analysis sequences, ensuring productivity in an applied testing laboratory. Based on the automatic and constant 5-times dilution of all samples, there are no matrix effects that would lead to unwanted interruptions of the analysis due to QC failures. Samples can be placed on the autosampler after completing preparation; no additional dilution is required. All types of interferences (polyatomic as well as isobaric interference, including doubly charged ions) are effectively suppressed with either helium collision gas or by using oxygen as a reactive gas in triple quadrupole mode. The instrument method could be easily set up thanks to the Reaction Finder method development assistant for <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1489"><strong>Qtegra ISDS Software</strong></a>.</p>

News from LabRulezICPMS Library - Week 4, 2025

<p><strong>In the week from January 27, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Agilent Technologies: Sample ID and Material Analysis by Handheld FTIR</strong></h5><div><div><ul><li><div>Tu, 28.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4645">Registration</a></div></li></ul></div><p>Learn how the Agilent 4300 can deliver an actionable result at site or in production in less than 30 seconds.</p></div><h5><strong>2.&nbsp;Mettler-Toledo: Routine Testing of Balances and Scales</strong></h5><div><div><ul><li><div>Tu, 28.1.2025 14:30 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4657">Registration</a></div></li></ul></div><p>This webinar will give you a good understanding of why balance and scale testing is crucial for the accuracy of your weighing results in laboratory and production environments.</p></div><h5><strong>3.&nbsp;Bruker: Ensure Food Authenticity &amp; Quality with NMR: Advanced Profiling Solutions</strong></h5><div><div><ul><li><div>We, 29.1.2025 10:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4659">Registration</a></div></li></ul></div><p>This webinar will delve into the basics of NMR, explore specific food profiler solutions, and highlight the versatility of NMR with different upgradation options.</p></div><h5><strong>4.&nbsp;SelectScience/Velp Scientifica: How to successfully face analytical challenges in fats and oils extractions</strong></h5><div><div><ul><li><div>We, 29.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4678">Registration</a></div></li></ul></div><p>Join Corey Letizio as he discusses how you can promptly address your challenges and streamline your extractions to benefit environmental, agricultural, and chemical sectors.</p></div><h5><strong>5.&nbsp;Mettler-Toledo: Live Webinar: How to Determine the Glass Transition by Thermal Analysis</strong></h5><div><div><ul><li><div>Th, 30.1.2025 08:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4549">Registration</a></div></li></ul></div><p>Join our webinar to discover the effectiveness of techniques such as Tg, DSC, TMA and DMA using a variety of application examples.</p></div><h5><strong>6.&nbsp;SPECTRO Analytical Instruments: High Precision Analysis with ICP-OES: Bracketing vs. Dynamic Measurement Correction</strong></h5><div><div><ul><li><div>Th, 30.1.2025 09:30 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4691">Registration</a></div></li></ul></div><p>High Precision Analysis with ICP-OES: Bracketing vs. Dynamic Measurement Correction - Join our live-webinar to discover all about this exciting techniques.</p></div>

Webinars LabRulezICPMS Week 5/2025

Synthesis and absolute configuration of cyclic synthetic cathinones derived from α-tetralone

<p><strong>In the week from February 3, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Mettler-Toledo: Improve Dairy Processing</strong></h5><div><div><ul><li><div>Tu, 4.2.2025 09:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4655">Registration</a></div></li></ul></div><p>The impact of pH on dairy production, issues with lab pH measurement, the value of continuous pH data and how the latest pH measurement systems can improve dairy processing.</p></div><h5><strong>2.&nbsp;Anton Paar: Optimizing Animal Feed Production: Mixing, Extrusion, Texture, and Tribology</strong></h5><div><div><ul><li><div>Tu, 4.2.2025 18:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4452">Registration</a></div></li></ul></div><p>This webinar will introduce both single and twin-screw extrusion techniques for lab-scale animal food production.</p></div><h5><strong>3.&nbsp;Agilent Technologies: Learn Why Atomic Absorption, MicroWave, and ICP Plasma Spectroscopy are the Perfect Tools for Both a Teaching and Research Environment</strong></h5><div><div><ul><li><div>Tu, 4.2.2025 19:00 (CET)</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/3158">Registration</a></div></li></ul></div><p>We will discuss each of the above techniques and outline the advantages and challenges of each.</p></div><h5><strong>4.&nbsp;Anton Paar: Scratch Measurement Optimization</strong></h5><div><div><ul><li><div>Tu, 4.2.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4450">Registration</a></div></li></ul></div><p>Explore how to fine-tune scratch test parameters for your specific needs.</p></div><h5><strong>5.&nbsp;Agilent Technologies: Atomic Spectroscopy</strong></h5><div><div><ul><li><div>Th, 6.2.2025 04:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4672">Registration</a></div></li></ul></div><p>In order to increase the effectiveness and productivity of elemental analysis in food and environmental samples, in this first session we will exchange tips and the newest Agilent technology available.</p></div><h5><strong>6.&nbsp;Anton Paar: Data Integrity - the Anton Paar way</strong></h5><div><div><ul><li><div>Th, 6.2.2025 10:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4551">Registration</a></div></li></ul></div><p>Data integrity has now been extended from the device to the AP Connect Pharma edition. This software allows you to store, verify, and validate your data easily in your own SQL database.</p></div><h5><strong>7.&nbsp;Separation Science/Agilent Technologies: Improving Efficiency with Challenging ICP-OES Applications Utilizing a Robust Sample Introduction System</strong></h5><div><div><ul><li><div>Th, 6.2.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4684">Registration</a></div></li></ul></div><p>In this webinar, we'll provide an overview of some new sample introduction components that remove pain points around running challenging sample matrices by ICP-OES efficiently.</p></div>

Webinars LabRulezICPMS Week 6/2025

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 3rd February 2025? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Shimadzu, Agilent Technologies </strong>and<strong> Thermo Fisher Scientific!</strong></p><h4><strong>1. Shimadzu: Simultaneous TG/DTA - DTG-60 Series</strong></h4><ul><li>Product note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c161_e005_0b449915ae.pdf">Full PDF for download</a></li></ul><p>This instrument varies the sample temperature in accordance with a program, and simultaneously measures the change in mass of the sample (TG) and the temperature difference between the sample and a standard substance (DTA).</p><ul><li>High-Sensitivity and High-Accuracy Measurement</li><li>Supports Various Types of Measurement</li><li>Automated Functions Increase Throughput</li><li>Complies with Analysis Laboratory Regulations</li><li>Compact Design</li></ul><h4><strong>2.&nbsp;Agilent Technologies: Capabilities and Operation of the Advanced Dilution System 2</strong></h4><ul><li>Technical Overview</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/te_ads_2_icp_5994_7211en_agilent_631a8d1650.pdf">Full PDF for download</a></li></ul><p><strong>Automate calibration and sample dilution for Agilent ICP-OES and ICP-MS instruments</strong></p><p>Now, more than ever, laboratories worldwide are facing increasing challenges to perform more analyses with fewer resources, prompting a re-evaluation of many analytical practices. Automation of certain tasks can help lab managers to streamline workflows and optimize resource utilization. To increase the efficiency of labs equipped with Agilent ICP-OES or ICP-MS instrumentation, Agilent has developed the Advanced Dilution System 2 (ADS 2)—a fully integrated inline autodilution system designed for routine, high throughput applications. The ADS 2 can automatically dilute stock standards and samples up to 400 times, making it ideal for the auto-preparation of calibration standards and samples, or dilution of overrange samples. The ADS 2 effectively reduces the number of manual tasks that operators have to perform when preparing a quantitative method for ICP-OES or ICP-MS, allowing more time to work on other tasks.</p><p>The two-syringe ADS 2 is available for the Agilent 5800 and 5900 ICP-OES* and Agilent 7850, 7900, and 8900 ICP-MS*. The ADS 2 and Advanced Valve System (AVS)** work seamlessly together under software control to maximize sample throughput, increase sample turn-around-time, and reduce cost-per-sample. The integrated design of the ADS 2 and AVS avoids adding excess time when not performing a dilution, addressing a common drawback of other dilution systems. The design also ensures that the system remains readily available to provide inline sample dilution whenever needed. If a reactive dilution of a sample is required, the intelligent software conveniently presents the results for the diluted sample at the end of each worksheet run, while retaining full access to all data.</p><h4><strong>3.&nbsp;Thermo Fisher Scientific: Thermo Scientific iCAP MX Series ICP-MS - Fully automatic start up and performance verification</strong></h4><ul><li>Product spotlight</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/sl_002910_tea_qtegra_isds_software_get_ready_sl002910_em_en_9e1bfd41ac.pdf">Full PDF for download</a></li></ul><p>The Thermo Scientific™ iCAP™ MX Series ICP-MS instruments provide sensitive, accurate, and robust analysis of a wide variety of sample matrices. Designed with ease of use and intelligent solutions to time-consuming steps in the daily laboratory in mind, the Get Ready process allows for automatic and unattended instrument initialization and performance verification.</p><p>Inductively coupled plasma-mass spectrometry (ICP-MS) is a powerful technology for trace and ultra-trace determination of elemental impurities in various markets, including environmental and food safety testing, industrial applications, clinical analysis, and research. In recent years, ICP-MS has become not only more sensitive to improve detection of lower concentrations, but also more robust to replace analyses previously run on ICP-optical emission spectroscopy (ICP-OES) instruments.</p><p>Another emerging trend is an increase in automation of labor-intensive and time-consuming steps in sample preparation and analysis. With increased demands on flexibility, both from clients demanding results with short turnover time as well as laboratory personnel looking for more flexible work arrangements, automated and timed start of the analytical system is an area to improve on efficiency and productivity in the laboratory.</p>

News from LabRulezICPMS Library - Week 6, 2025

<p><strong>In the week from February 10, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Merck: Webinar Series &amp; Expert Roundtable (Day 1)</strong></h5><ul><li>Monday, February 10, 2025, 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4647">Registration</a></li></ul><p>Webinar Series &amp; Expert Roundtable (Day 1) - Navigating Quality Grades Part 1: Inorganics and Solvents for Classical Analysis and Research and Part 2: Solvents and Reagents for Chromatography Applications</p><h5><strong>2. Merck: Webinar Series &amp; Expert Roundtable (Day 2)</strong></h5><ul><li>Tuesday, February 11, 2025, 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4646">Registration</a></li></ul><p>Webinar Series &amp; Expert Roundtable (Day 2) - Part 1: Dihydropinene, γ-Valerolactone, and Dimethyl Isosorbide: Solvents for Organic Synthesis and Part 2: Enhance Your PFAS Testing</p><h5><strong>3. Mettler-Toledo: Laboratory Balance Cleaning</strong></h5><ul><li>Tuesday, February 11, 2025, 10:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4654">Registration</a></li></ul><p>Join our free live webinar to find out more about the benefits of and solutions for cleaning your balance.</p><h5><strong>4. Thermo Fisher Scientific: Increasing Matrix Robustness using Intelligent Matrix Handling (IMH) with Thermo Scientific iCAP MX Series ICP-MS</strong></h5><ul><li>Tuesday, February 11, 2025, 13:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4698">Registration</a></li></ul><p>Join this virtual demonstration of the Thermo Scientific iCAP MX Series ICP-MS to learn how Qtegra ISDS Intelligent Matrix Handling reduces sample-to-sample carryover and maintenance of your instrument.</p><h5><strong>5. Agilent Technologies: From Single Cell and Nanoparticle Analysis to Laser Ablation and Beyond: Advanced ICP-MS Research Applications</strong></h5><ul><li>Tuesday, February 11, 2025, 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/3159">Registration</a></li></ul><p>Explore nanoparticle, single cell, speciation, laser ablation, and other hyphenated ICP-MS applications that can advance your research.</p><h5><strong>6. Merck: Webinar Series &amp; Expert Roundtable (Day 3)</strong></h5><ul><li>Wednesday, February 12, 2025, 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4648">Registration</a></li></ul><p>Webinar Series &amp; Expert Roundtable (Day 3) - Part 1: Optimize Your Karl Fischer Titration and Part 2: Beyond Safety Standards – Best Practices for Safe Handling of Hazardous Chemicals</p><h5><strong>7. C&amp;EN/Merck: From Challenges to Solutions: Revolutionizing Moisture Analysis with NIR and Titration</strong></h5><ul><li>Wednesday, February 12, 2025, 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4731">Registration</a></li></ul><p>Join industry experts as they explore modern approaches to moisture analysis in chemical, polymer, and petrochemical environments.</p><h5><strong>8. Merck: Webinar Series &amp; Expert Roundtable (Day 4)</strong></h5><ul><li>Thursday, February 13, 2025, 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4649">Registration</a></li></ul><p>Webinar Series &amp; Expert Roundtable (Day 4) - Part 1: Greener Solvents - Making Big Moves for More Sustainability in the Lab! and Part 2: Tips and Tricks for LC-MS Troubleshooting.</p><h5><strong>9. LECO: Kjeldahl vs Dumas - How Do You Determine Protein?</strong></h5><ul><li>Thursday, February 13, 2025, 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4685">Registration</a></li></ul><p>Join us for this 30-minute webinar where Michael Jakob, LECO European Product Manager, discusses how LECO’s protein analyzers streamline the Dumas method for protein determination. The webinar also features an online demonstration of running a sample on the FP828 in our Berlin lab.</p><h5><strong>10. Agilent Technologies: New Improvements to MP Workflow</strong></h5><ul><li>Thursday, February 13, 2025, 20:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4552">Registration</a></li></ul><p>Improvements to Microplastics workflow will be demonstrated.</p><h5><strong>11. Merck: Webinar Series &amp; Expert Roundtable (Day 5)</strong></h5><ul><li>Friday, February 14, 2025, 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4650">Registration</a></li></ul><p>Webinar Series &amp; Expert Roundtable (Day 5) - Roundtable Expert Discussion.</p>

Webinars LabRulezICPMS Week 7/2025

<p><strong>This study introduces a simple and absolute method called evaporated volume analysis (EVA) for determining the number concentration of nanoparticles. The technique involves depositing macroscopic volumes of nanoparticle dispersions onto a glass surface, allowing the solvent to evaporate, and subsequently scanning the entire dried droplet with an optical microscope. Stitched micrographs enable AI-assisted counting of thousands of nanoparticles per droplet.&nbsp;</strong></p><p>The method was validated using ∼60 nm Tm³⁺-doped photon-upconversion nanoparticles, achieving a combined relative standard uncertainty of 2.7%, and was further tested with ∼80 nm Nile red-doped polystyrene and ∼90 nm silver nanoparticles. Theoretical performance limits, including detection limits, quantification limits, and optimal working ranges, are also discussed.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c05555"><strong>Optical Microscopy and Deep Learning for Absolute Quantification of Nanoparticles on a Macroscopic Scale and Estimating Their Number Concentration</strong></a></h4><p>Antonín Hlaváček, Kateřina Uhrová, Julie Weisová, Hana Brožková, Naděžda Pizúrová</p><p>Anal. Chem. 2025, 97, 5, 2588–2592</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.4c05555">https://doi.org/10.1021/jasms.4c00455</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p>Many new substances─nanoparticles─have been recently prepared and advanced research and technology. They also compose our environment and bodies where part of them is of natural origin and the other part is contributed by human activity. Besides others, the absolute quantification of nanoparticles and estimating their number concentrations (<i>i</i>.<i>e</i>. the number of nanoparticles in a given volume) are important for standardization, trading, studying nanoparticle properties, researching their role in the environment, and evaluating their toxicity. <a href="javascript:void(0);" aria-label="reference 1 and 2">(1,2)</a> Available techniques for nanoparticle quantification measure either the ensemble property of nanoparticle dispersion or utilize nanoparticle counting. <a href="javascript:void(0);" aria-label="reference 1 and 2">(1,2)</a> The counting is absolute and more straightforward. <a href="javascript:void(0);" aria-label="reference 1 and 2">(1,2)</a> Two types of counting approaches─serial and parallel─can be recognized. In serial counting, the nanoparticles are detected individually when the dispersion streams through a detector. Optical detection, <a href="javascript:void(0);" aria-label="reference 3">(3)</a> resistive pulse sensing, <a href="javascript:void(0);" aria-label="reference 4">(4)</a> or single particle mass spectroscopy <a href="javascript:void(0);" aria-label="reference 5">(5)</a> can be used. In a parallel setting, microscopy is used for imaging and counting large numbers of nanoparticles in a single micrograph. For instance, optical microscopy was used for counting and tracking single nanoparticles in a free dispersion. <a href="javascript:void(0);" aria-label="reference 6">(6)</a></p><p>However, these methods are limited by short detection times, which prevent us from counting small nanoparticles (∼30 nm) with generally low signals. <a href="javascript:void(0);" aria-label="reference 6 and 7">(6,7)</a> In serial counting, the detection time is limited by a need for sufficiently fast flow and diffusion of nanoparticles imposes limits in single-particle tracking. The problem of moving nanoparticles was solved by their embedding in gels <a href="javascript:void(0);" aria-label="reference 8">(8)</a> or resins. <a href="javascript:void(0);" aria-label="reference 9">(9)</a> However, the detection is still limited by a background signal <a href="javascript:void(0);" aria-label="reference 7">(7)</a> from the gel-enclosed solvent, gel matrix, or resin, and the complexity of instrumentation. <a href="javascript:void(0);" aria-label="reference 8 and 9">(8,9)</a> To overcome these limitations, specialized sampling approaches are developed when the nanoparticles are immobilized on a suitable substrate and the solvent is removed traceably. For instance, a nanopipette was designed for transmission electron microscopy (TEM), <a href="javascript:void(0);" aria-label="reference 10">(10)</a> and anisotropically collapsing gels were used for optical microscopy. <a href="javascript:void(0);" aria-label="reference 11">(11)</a></p><p>Here, we present a simplistic approach (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c05555#sch1" aria-label="Scheme 1">Scheme 1</a>). A macroscopic volume of nanoparticle dispersion is dropped on a glass surface and the solvent is freely evaporated. An optical microscope scans the dried droplet, and all nanoparticles are counted. We call this method evaporated volume analysis (EVA) because all nanoparticles are counted after droplet volume evaporation. Indeed, the EVA is not a completely new technique and was used either for larger particles with specific imaging signals (<i>e</i>.<i>g</i>. 107 nm Eu chelate-doped polystyrene nanoparticles in time-resolved fluorescence microscope) <a href="javascript:void(0);" aria-label="reference 12">(12)</a> or for counting particles from microscopic droplets fitting the single field of view of the microscope (<i>e</i>.<i>g</i>. gold nanoparticles in piezo-dispensed microdroplets in scanning electron microscope). <a href="javascript:void(0);" aria-label="reference 13">(13)</a> On the other hand, theoretical limits, and uncertainty sources were not investigated. The EVA was also overlooked in reviews dedicated to the methods of nanoparticle quantification. <a href="javascript:void(0);" aria-label="reference 1 and 2">(1,2)</a> The reasons for this limited use can be (1) contradicting requirements for sensitivity and the field of view (tends to image either large particles or working with impractically small volumes/fields of view), <a href="javascript:void(0);" aria-label="reference 7">(7)</a> (2) the need for counting large numbers of nanoparticles (counting uncertainty decreases with number of counted nanoparticles), (3) nanoparticle spatial organization during the droplet drying (causing nanoparticle overlaps and subsequent miscounting). <a href="javascript:void(0);" aria-label="reference 14">(14)</a> We solved these problems by (1) stitching multiple micrographs of small fields of view ultimately covering macroscopic scales, (2) artificial intelligence automated the counting of nanoparticles, and (3) nanoparticle clustering was prevented by introducing ultralow gelling agarose. As a model, an aqueous dispersion of ∼60 nm Tm<sup>3+</sup>-doped photon-upconversion nanoparticles (UCNPs) coated in carboxylated silica shells (UCNP-COOHs) are quantified. Uncertainty sources are discussed and theoretical limits are analyzed. A wider applicability is tested by imaging ∼80 nm Nile red-doped polystyrene nanoparticles (NileNPs) and ∼90 nm silver nanoparticles (AgNPs). See the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c05555/suppl_file/ac4c05555_si_001.pdf">Supporting Information</a> (SI) for nanoparticle synthesis (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c05555/suppl_file/ac4c05555_si_001.pdf">SI Note S1</a>) and characterization (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c05555/suppl_file/ac4c05555_si_001.pdf">SI Note S2</a>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_5_2588_2592_Scheme_1_4720dbde56.jpeg" alt="Anal. Chem. 2025, 97, 5, 2588–2592 - Scheme 1 a) Nanoparticles (blue dots) in ultra-low-gelling agarose  b) After gelling and drying, c) the entire droplet is covered with a grit of 6×6 images, the images are stitched, and nanoparticles are counted" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_5_2588_2592_Scheme_1_4720dbde56.jpeg 245w," sizes="100vw" width="1000" height="583"></p><p>TEM showed ∼60 nm oleic acid-capped NaYF<sub>4</sub> UCNPs doped with 18% Yb<sup>3+</sup> and 2% Tm<sup>3+</sup> (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c05555/suppl_file/ac4c05555_si_001.pdf">Figure S1, SI Note S2</a>). The UCNP-COOHs were formed by coating the UCNPs with a ∼ 5 nm thick carboxylated silica <a href="javascript:void(0);" aria-label="reference 15">(15)</a> (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c05555#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>a, <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c05555/suppl_file/ac4c05555_si_001.pdf">Figure S2, SI Note S2</a>) and were stored as a concentrated aqueous dispersion (11.8 ± 0.24 mg mL<sup>–1</sup>). The sizes were confirmed by dynamic light scattering estimating hydrodynamic diameters at 69 and 78 nm for oleic acid-capped UCNPs and UCNP-COOHs, respectively (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c05555#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>b). The dispersion of UCNP-COOHs was virtually aggregate-free as confirmed by agarose gel electrophoresis (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c05555#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>c). Under the excitation of 976 nm, the UCNP-COOH emitted strongly at near-infrared (802 nm, <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c05555#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>d), and in the epiphoton-upconversion microscope appeared as diffraction-limited spots with a full width at half-maximum of 1.8 px (1.2 μm, <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c05555#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>e,f, <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c05555/suppl_file/ac4c05555_si_001.pdf">SI Note S3, Figure S3</a>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_5_2588_2592_Figure_1_eb43eecb83.jpeg" alt="Anal. Chem. 2025, 97, 5, 2588–2592 - Figure 1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_5_2588_2592_Figure_1_eb43eecb83.jpeg 104w," sizes="100vw" width="1000" height="1504"></p><p><i>Nanoparticle properties. (a) TEM image of UCNP-COOHs. (b) Hydrodynamic diameters of UCNPs with different surface modifications. (c) UCNP-COOH agarose electrophoresis. (d) The emission spectrum of UCNP-COOHs in water. (e) Epiphoton-upconversion microscopy of UCNP-COOHs in the dried droplet (exposure 5 s). (f) Nanoparticle spot cross sections as indicated in (e). (c-f) Excitation 976 nm. Emission (c) 764–822 nm, and (e,f) 800 ± 25 nm.</i></p><h3><strong>Conclusion</strong></h3><p>In conclusion, image stitching, artificial neural networks, and agarose gel refined the properties of EVA. The number concentration of ∼60 nm UCNPs was estimated with a relative standard uncertainty of 2.7%. Imaging fluorescent NileNPs and plasmonic AgNPs confirmed the wider applicability.</p>

Optical Microscopy and Deep Learning for Absolute Quantification of Nanoparticles on a Macroscopic Scale and Estimating Their Number Concentration

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 10th February 2025? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Shimadzu, Agilent Technologies </strong>and<strong> Thermo Fisher Scientific!</strong></p><h4><strong>1. Shimadzu: Analyzing Various Plastics</strong></h4><p><strong>Benefits of Bundled KnowItAll® Software</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00824_en_3f3cdee3b3.pdf"><strong>Full PDF for download</strong></a></li></ul><p>This article describes using John Wiley &amp; Sons, Inc. <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?search=KnowItAll"><strong>KnowItAll software </strong></a>for plastic analysis. KnowItAll has an extensive library for a wide variety of data analysis, such as searching for multiple components to identify and determine the percentage of chemical components contained in plastics or identifying<br>functional groups attributable to specific spectral peaks (partial structural analysis). Furthermore, KnowItAll is bundled with Shimadzu <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=3&amp;search=LabSolutions"><strong>LabSolutions IR software</strong></a> for <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=3&amp;search=FTIR"><strong>FTIR analysis</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=3&amp;search=AMsolution"><strong>AMsolution</strong></a> infrared microscope software. Clicking the [KnowItAll] button in the Shimadzu software automatically sends the active spectral data to the KnowItAll software, ensuring quick analysis.</p><h5><strong>Conclusion</strong></h5><p>An FTIR system in combination with a single-reflection <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=3&amp;search=ATR"><strong>ATR accessory</strong></a> can measure a variety of plastics. However, the analyst must be able to judge whether the software data analysis results are correct, which requires significant amounts of knowledge and experience. But with KnowItAll bundle described in this article, functional groups of specific peaks or multiple components can be easily analyzed, providing an effective way to improve the accuracy of data analysis.</p><h4><strong>2. Shimadzu: Speciation Analysis of Chromium by LC-ICP-MS Based on ISO 24384</strong></h4><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00809_en_13bfd5870a.pdf"><strong>Full PDF for download</strong></a></li></ul><p><strong>User Benefits:</strong></p><ul><li>Enables Cr(III) and Cr(VI) to be measured simultaneously in accordance with ISO 24384.</li><li>Suffers less interference from coexisting substances than the conventional method for measuring Cr(VI) and can be analyzed with<br>a simple pretreatment.</li><li>Easy to connect instruments simply by attaching the LC column outlet piping to the ICP-MS nebulizer.</li></ul><p>In this method, Cr(III) is chelated using 2,6-pyridinedicarboxylic acid (PDCA) or ethylenediaminetetraacetic acid (EDTA) and then separated and quantified by <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=3&amp;search=LC-ICP-MS"><strong>LC-ICP-MS</strong></a>. This method is less prone to interference, is simple to use , and allows simultaneous determination of Cr(III) and Cr(VI). In this application, EDTA was used as a chelating agent, and the results of performing chromium speciation using a Prominence Inert Analysis System connected to an <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=3&amp;search=ICPMS-2050"><strong>ICPMS-2050</strong></a> are reported.</p><h5><strong>Conclusion</strong></h5><p>The measurement sensitivity and resolution were good, and the spike recovery rate for each sample met the criteria required by ISO 24384 well. The LC-ICP-MS method can simultaneously quantify Cr(III) and Cr(VI) using only simple pretreatment and is less prone to the effects of coexisting substances than the conventional method. The LC-ICP-MS analysis system is easily constructed by simply attaching the LC column outlet piping to the ICP-MS nebulizer. This analysis was performed on an ICPMS-2050 equipped with a collision/reaction cell, but it can also be performed on the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=3&amp;search=ICPMS-2040"><strong>ICPMS-2040</strong></a>, a model dedicated to the collision mode.</p><h4><strong>3. Agilent Technologies: Elemental Analysis of Lithium-Ion Battery Black Mass Recycling Material by ICP-OES&nbsp;</strong></h4><p><strong>Enhancing instrument robustness for the analysis of high-TDS samples using an inert Agilent V-groove nebulizer</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_battery_black_mass_recycling_icp_oes_5994_8064en_agilent_1_09aa5f70f0.pdf"><strong>Full PDF for download</strong></a></li></ul><p>This study highlights the effectiveness of the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=1&amp;search=5900+svdv"><strong>Agilent 5900 SVDV ICP-OES</strong></a>, equipped with the Agilent inert V-groove nebulizer, for the multi-element analysis of acid-digests of black mass (BM) samples. This new nebulizer enables routine analysis of unfiltered samples with up to 30% total dissolved solids and suspended particles of up to 350 μm in size. The system demonstrated an outstanding long-term stability over 16 hours and excellent spike recoveries. Additionally, the V-groove nebulizer operated without blockages for over three weeks, reducing maintenance requirements while ensuring high accuracy and precision. This method offers BM producers a reliable solution for directly analyzing key analytes in unfiltered sample digests, supporting efforts to recover valuable metals from spent lithium-ion batteries.</p><h5><strong>Conclusion</strong></h5><p>This study shows the suitability of the Agilent 5900 SVDV ICP-OES equipped with the Agilent inert V-groove nebulizer for the multi-element analysis of unfiltered acid-digests of black mass (BM) samples. The robust nebulizer is recommended for the routine analysis of samples with up to 30% total dissolved solids (TDS) and particle sizes less than 350 µm. Excellent long-term stability was demonstrated when samples were analyzed over 16 hours. Measurements of the QC solution were within ± 10% of the expected value, with precision better than 5% RSD. The spike recoveries were well within ± 10% of the spiked concentration at both low and high concentration levels, confirming the accuracy of the method for the detection of a wide range of elemental concentrations in a single analysis. The V-groove nebulizer enabled the samples to be run for more than three weeks without any blockages, while maintaining a high level of accuracy and reducing the frequency of torch and nebulizer maintenance. With an increased focus on recovering valuable metals from spent lithium-ion batteries, BM producers can confidently analyze key analytes in unfiltered sample digests directly using the V-groove nebulizer to enhance the robustness of the 5900 ICP-OES.</p><h4><strong>4. Thermo Fisher Scientific: Supporting development and production with elemental analysis in life sciences and beyond</strong></h4><ul><li>Case study</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cs_003219_tea_icp_ms_icap_mx_series_merck_cs003219_na_en_8e346da1d8.pdf"><strong>Full PDF for download</strong></a></li></ul><p>The central analytics laboratory at Merck KGaA in Darmstadt, Germany, provides elemental analysis services to researchers and production units across the global Merck Group. Led by Dr. Sven Traxel, the Element Analytics group operates ten ICP-MS instruments to handle approximately 3,000 analysis requests annually, supporting development and troubleshooting in various fields. Their work encompasses a diverse range of sample matrices, including pharmaceuticals, life sciences products, liquid crystals, OLEDs, semiconductor materials, specialty chemicals, and pigments. Sample preparation methods vary from microwave-assisted digestions to open vessel digestions using different acids, with analyses often conducted overnight for efficiency. The team has been assessing the Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?manufacturerItems=2&amp;search=iCAP+MSX+ICP-MS"><strong>iCAP™ MSX ICP-MS</strong></a> for its suitability in their analyses.</p><p>ICP-MS expert Niklas Brenner, responsible for developing and adapting measurement methods, has extensive experience with various instruments, including the Thermo Scientific™ iCAP™ Q ICP-MS. He notes that the iCAP MSX ICP-MS delivers performance comparable to other instruments in their laboratory. A significant advantage is the Argon Gas Dilution (AGD) feature, which reduces matrix effects and allows for a higher number of samples to be run before maintenance is required. Brenner reports analyzing nearly 1,500 samples with consistent performance since installation. While AGD slightly reduces system sensitivity, it does not compromise the laboratory's ability to achieve required detection limits. Routine maintenance, such as cleaning the cones, typically necessitates 2–3 hours of downtime weekly, but the iCAP MSX ICP-MS's design helps minimize this, enhancing overall laboratory efficiency.</p>

News from LabRulezICPMS Library - Week 7, 2025

<p><strong>In the week from February 17, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5 data-start="514" data-end="566"><strong data-start="520" data-end="566">1. Anton Paar: Advanced Scratch Techniques</strong></h5><ul data-start="568" data-end="622"><li data-start="568" data-end="607">Tuesday, February 18, 2025, 20:00 CET</li><li data-start="608" data-end="622"><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4451">Registration</a></li></ul><p data-start="624" data-end="781">In this webinar of our Scratch Series, we introduce advanced quantitative techniques to deepen your understanding of material behavior under scratch testing.</p><h5 data-start="783" data-end="898"><strong data-start="789" data-end="898">2. New Food Magazine/Bruker: Enhancing Food Safety Labs with Rapid Detection of Salmonella, Listeria, and Moulds</strong></h5><ul data-start="900" data-end="956"><li data-start="900" data-end="941">Wednesday, February 19, 2025, 16:00 CET</li><li data-start="942" data-end="956"><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4723">Registration</a></li></ul><p data-start="958" data-end="1105">This webinar focuses on advanced methodologies for detecting microorganisms, including rapid techniques such as MALDI-TOF MS and FTIR-spectroscopy.</p><h5 data-start="1107" data-end="1164"><strong data-start="1113" data-end="1164">3. Agilent Technologies: Molecular Spectroscopy</strong></h5><ul data-start="1166" data-end="1221"><li data-start="1166" data-end="1206">Thursday, February 20, 2025, 04:00 CET</li><li data-start="1207" data-end="1221"><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4673">Registration</a></li></ul><p data-start="1223" data-end="1324">In this second session, we will talk about the analysis of microplastics in the environment and food.</p><h5 data-start="1326" data-end="1490"><strong data-start="1332" data-end="1490">4. Separation Science/Agilent Technologies: Eco-Friendly Extraction Methods for Lithium-Ion Battery Recycling and Elemental Analysis of Impurities in Recovered Metal Sulphates</strong></h5><ul data-start="1492" data-end="1547"><li data-start="1492" data-end="1532">Thursday, February 20, 2025, 11:00 CET</li><li data-start="1533" data-end="1547"><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4708">Registration</a></li></ul><p data-start="1549" data-end="1744">Join us for an insightful webinar on sustainable lithium-ion battery recycling! Discover greener solvents and learn how ICP-OES seamlessly handles alternatives to traditional inorganic solutions.</p><h5 data-start="1746" data-end="1852"><strong data-start="1752" data-end="1852">5. Agilent Technologies: Tips and Tricks on ICP-OES for Best Detection Limits and Less Carryover</strong></h5><ul data-start="1854" data-end="1909"><li data-start="1854" data-end="1894">Thursday, February 20, 2025, 19:00 CET</li><li data-start="1895" data-end="1909"><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/3162">Registration</a></li></ul><p data-start="1911" data-end="2041">Learn all the tips and tricks and select proper components of an ICP-OES sample introduction system to optimize your data quality.</p>

Webinars LabRulezICPMS Week 8/2025

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