<h3><strong>Conference Program</strong></h3>
<h3><strong>13th October 2021 (Wednesday)</strong></h3>
<h5><strong>7:30 - 8:20 REGISTRATION</strong></h5>
<h5><strong>8:20 - 8:40 OPENING OF THE CONFERENCE</strong></h5>
<h4><strong>SESSION I (in NLT)</strong></h4>
<p>(Chair: František Tureček (University of Washington, United States))</p>
<h5><strong>8:40 - 9:30 Computational and Gas-phase Studies in Service of Catalysis Research: Identification of the &quot;Aristocratic Atoms&quot;</strong></h5>
<ul>
<li><em>Helmut Schwarz (Technische Universität Berlin, Germany)</em><h5><strong>9:30 - 10:00 Mass Spectrometry at the J. Heyrovský Institute of Physical Chemistry</strong></h5>
</li>
<li><em>Miroslav Polášek (Heyrovský Institute of Physical Chemistry of the CAS, Czech Republic)</em><h5><strong>10:00 - 10:30 Gas Phase Ion-molecule Reactions in Czechia over 60 Years</strong></h5>
</li>
<li><em>Patrik Španěl (Heyrovský Institute of Physical Chemistry of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>COFFEE BREAK</strong></h5>
<h4><strong>SESSION II (in NLT)</strong></h4>
<p>(Chair: <a href="https://lcms.cz/articles?search=michal+holcapek">Michal Holčapek</a> (<a href="https://lcms.cz/companies/15">University of Pardubice</a>, Czech Republic))</p>
<h5><strong>11:00 - 11:30 Past, Present and Future of Orbitrap Mass Spectrometry (virtual presentation)</strong></h5>
<ul>
<li><em>Alexander Makarov (Thermo Fisher Scientific, Germany)</em></li>
</ul>
<h5><strong>11:30 - 12:00 Development of Novel Ion-less Matrices for Profiling and Imaging Mass Spectrometry</strong></h5>
<ul>
<li><em>Aleš Svatoš (Max Planck Institute for Chemical Ecology, Germany)</em></li>
</ul>
<h5><strong>12:00 - 12:30 Mass Spectrometry at the Institute of Organic Chemistry and Biochemistry &amp; Museum Opening</strong></h5>
<ul>
<li><em>Josef Cvačka (Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>LUNCH BREAK</strong></h5>
<h4><strong>SESSION III (in NLT)</strong></h4>
<p>(Chair: Miroslav Polášek (Heyrovský Inst. of Physical Chemistry of the <a href="https://lcms.cz/companies/10">CAS</a>, Czech Republic))</p>
<h5><strong>14:00 - 14:50 High-resolution Native Mass Spectrometry</strong></h5>
<ul>
<li><em>Albert J. R. Heck (Utrecht University, The Netherlands)</em></li>
</ul>
<h5><strong>14:50 - 15:20 Noncanonical Ionized DNA Nucleobases and Nucleosides. A Dark Matter of DNA Ionization?</strong></h5>
<ul>
<li><em>František Tureček (University of Washington, United States)</em></li>
</ul>
<h5><strong>15:20 - 15:50 Mass Spectrometry at the University of Pardubice: Focus on Lipidomics</strong></h5>
<ul>
<li><em>Michal Holčapek (University of Pardubice, Czech Republic)</em></li>
</ul>
<h5><strong>15:50 - 16:20 Forty Year Evolution of High Throughput Mass Spectrometry: A Perspective (virtual presentation)</strong></h5>
<ul>
<li><em>Tom Covey (SCIEX, Canada)</em></li>
</ul>
<h5><strong>BREAK</strong></h5>
<h4><strong>POSTER SESSION &amp; DINNER (in OWTP)</strong></h4>
<h5><strong>17:30 - 19:00 POSTER SESSION</strong></h5>
<h5><strong>19:00 - 23:00 SOCIAL EVENT - Conference dinner, concert performance, guided underground tours</strong></h5>
<h3><strong>14th October 2021 (Thursday)</strong></h3>
<h4><strong>SESSION IV (in NLT)</strong></h4>
<p>(Chair: <a href="https://lcms.cz/articles?search=jan+preisler">Jan Preisler</a> (<a href="https://lcms.cz/companies/12">Masaryk University</a>, Czech Republic))</p>
<h5><strong>8:40 - 9:30 FT ICR Mass Spectrometry. Is There Room for Further Improvements? (virtual presentation)</strong></h5>
<ul>
<li><em>Evgeny Nikolaev (Skolkovo Institute of Science and Technology, Russia)</em></li>
</ul>
<h5><strong>9:30 - 10:00 Mass Spectrometry: from Metabolites to Art</strong></h5>
<ul>
<li><em>Karel Lemr (Palacký University Olomouc, Czech Republic)</em></li>
</ul>
<h5><strong>10:00 - 10:30 Historical Perspectives on Bruker’s Involvement in Mass Spectrometry and the Development of Modern Q-TOF Mass Spectrometry</strong></h5>
<ul>
<li><em>Gary H. Kruppa (Bruker, Czech Republic)</em></li>
</ul>
<h5><strong>COFFEE BREAK</strong></h5>
<h4><strong>SESSION V – YOUNG SCIENTISTS (in NLT)</strong></h4>
<p>(Chair: <a href="https://lcms.cz/articles?search=karel+lemr">Karel Lemr</a> (<a href="https://lcms.cz/companies/17">Palacký University Olomouc</a>, Czech Republic))</p>
<h5><strong>11:00 - 11:15 Identification of Novel Fatty Acids in Vernix Caseosa using UV Photodissociation Mass Spectrometry in Combination with Fixed-charge Photo-labile Derivatives</strong></h5>
<ul>
<li><em>Lukáš Cudlman (Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>11:15 - 11:30 Mass Spectrometry-based Chemical Proteomics in Protein Post-translational Modifications Analysis</strong></h5>
<ul>
<li><em>Pavel Kielkowski (Ludwig-Maximilians-Universität München, Germany)</em></li>
</ul>
<h5><strong>11:30 - 11:45 Mapping of Regional and Subregional Unbound Drug Transport Across Blood-Brain Barrier Enabled by Quantitative Mass Spectrometry Imaging</strong></h5>
<ul>
<li><em>Dominika Luptáková (Uppsala University, Sweden)</em></li>
</ul>
<h5><strong>11:45 - 12:00 Visualization of Polyamines and Amino Acids Alterations in Neonatal Brain Hypoxic-Ischemic Injury in Rats by Mass Spectrometry Imaging</strong></h5>
<ul>
<li><em>Hynek Mácha (Institute of Microbiology of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>12:00 - 12:15 Temperature-Controlled Electrospray: A Window into Solution Thermochemistry of Non-Canonical Nucleic Acid Complexes</strong></h5>
<ul>
<li><em>Adam Pruška (ETH Zurich, Switzerland)</em></li>
</ul>
<h5><strong>12:15 - 12:30 Freeing Aspergillus fumigatus of Polymycovirus Infection renders it More Resistant to Competition with Pseudomonas aeruginosa due to Altered Iron-acquiring Tactics</strong></h5>
<ul>
<li><em>Rutuja Patil (Institute of Microbiology of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>LUNCH BREAK</strong></h5>
<h4><strong>SESSION VI (in NLT)</strong></h4>
<p>(Chair: Aleš Svatoš (Max Planck Institute for Chemical Ecology, Germany))</p>
<h5><strong>14:00 - 14:50 Native Ambient Mass Spectrometry: Mass Spectrometry Imaging of Intact Proteins and Protein Complexes (virtual presentation)</strong></h5>
<ul>
<li><em>Helen J. Cooper (University of Birmingham, United Kingdom)</em></li>
</ul>
<h5><strong>14:50 - 15:20 A (Time of) Flight from Ames to Brno: Laser Desorption Mass Spectrometry at Masaryk University</strong></h5>
<ul>
<li><em>Jan Preisler (Masaryk University, Czech Republic)</em></li>
</ul>
<h5><strong>15:20 - 15:50 The History of Time-of-Flight Mass Spectrometry at: VG - Micromass - Waters (virtual presentation)</strong></h5>
<ul>
<li><em>Mike Morris (Waters Corporation, Manchester, United Kingdom)</em></li>
</ul>
<h5><strong>15:50 - 16:20 Mass Spectrometry Innovation at Agilent Technologies (virtual presentation)</strong></h5>
<ul>
<li><em>George Stafford (Agilent Technologies, United States)</em></li>
</ul>
<h5><strong>16:20 - 16:30 CONFERENCE CLOSING</strong></h5>
<h4><strong>IOCB ChemBioDrug Workshop (in NLT</strong>)</h4>
<p>(Chair: <a href="https://lcms.cz/articles?search=josef+cvacka"> Josef Cvačka</a> (<a href="https://lcms.cz/companies/62">IOCB</a>, Czech Republic))</p>
<h5><strong>17:00 - 17:05 OPENING</strong></h5>
<h5><strong>17:05 - 17:25 Orbitrap Fusion Lumos: A Versatile Mass Spectrometer for Biomolecules</strong></h5>
<ul>
<li><em>Martin Hubálek (Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>17:25 - 17:45 Protein Structure and Interactions Studied by Cross-Linking Mass Spectrometry</strong></h5>
<ul>
<li><em>Petra Junková (Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>17:45 - 18:05 Protein Structure and Interactions Studied by Hydrogen Deuterium Exchange Mass Spectrometry</strong></h5>
<ul>
<li><em>Jakub Sýs (Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>18:05 - 18:30 HPLC/ESI-MS2 – Based Lipidomic Analysis and MALDI Imaging of Lipids</strong></h5>
<ul>
<li><em>Vladimír Vrkoslav, Štěpán Strnad (Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic)</em></li>
</ul>
<h5><strong>18:30 - 19:00 Discussion / User Meeting</strong></h5>
<h5><strong>WORKSHOP CLOSING</strong></h5>
<p>Program je k dispozici také jako pdf na webu <a href="http://ms-prague2021.uochb.cas.cz/program.php">Prague Meeting on Historical Perspectives of Mass Spectrometry</a></p>


Conference Program Prague Meeting on Historical Perspectives of Mass Spectrometry

<p><strong>We would like to cordially invite you to the joint meeting of the CZECH-SLOVAK SPECTROSCOPIC CONFERENCE and MÖSSBAUER SPECTROSCOPY IN MATERIAL SCIENCES 2024.</strong></p>
<h3><a href="https://icpms.labrulez.com/events/479"><strong>18th Czech - Slovak Spectroscopic Conference (CSSC)</strong></a></h3>
<p><strong>organized by Ioannes Marcus Marci Spectroscopic Society  &amp;  Slovak Spectroscopic Society</strong> (a member of the Association of Slovak Scientific and Technological Societies)</p>
<p><strong>&amp;</strong></p>
<h3><a href="https://icpms.labrulez.com/events/479"><strong>Mössbauer Spectroscopy in Materials Science (MSMS)</strong></a></h3>
<p><strong>organized by Ioannes Marcus Marci Spectroscopic Society  &amp;  Palacky University Olomouc &amp; Slovak Spectroscopic Society</strong> (a member of the Association of Slovak Scientific and Technological Societies)</p>
<p><strong>Will take place in Kurdějov, May 27 - 31,  2024</strong></p>
<h3><strong>Microsymposium as a Conference Programme Session</strong></h3>
<p><strong>&quot;Recent progress in vibrational spectroscopy - Celebrating 50 years of SERS and the 65th anniversary of A. Smekal death&quot;</strong></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSSC_and_MSMS_2024_banner_445de982f8/CSSC-and-MSMS-2024-banner.jpg" alt="CSSC &amp; MSMS 2024"/></p>
<blockquote>
<h3><a href="http://cssc2024.spektroskopie.cz/registration_online"><strong>ONLINE REGISTRATION</strong></a></h3>
<ul>
<li><strong>Early fee</strong> (registration before March 15, 2024)</li>
<li><strong>Regular fee</strong> (registration after March 15, 2024)</li>
</ul>
</blockquote>
<blockquote>
<h3><a href="http://cssc2024.spektroskopie.cz/"><strong>OFFICIAL COINFERENCE WEBPAGE</strong></a></h3>
</blockquote>
<p> <strong>The 18th Czech-Slovak Spectroscopic Conference (18th CSSC)</strong> is the scientific meeting with international participation to share recent developments, exchange ideas, explore new directions and initiate a possible collaboration in the <strong>analytical spectrometry</strong> area. Leading scientists and researchers will be invited to present their most up-to-date results at this conference, to exchange exciting ideas and experiences as well as look into future development. The aim of this scientific event is to <strong>bring together experts</strong> from universities, academia, official centers, various laboratories, and industry, to summarize the current progress in various areas of spectroscopy/spectrometry and the trends in the applications such as chemical, environmental, geological, biological, food, pharmaceutical and industrial materials and to stimulate contacts and mutual exchange of experiences and ideas.</p>
<p>The upcoming year of this conference with a long tradition differs from the previous ones by a greater emphasis on analytical spectrometry applied in the <strong>geological sciences</strong>. Many scientists with a geological background are engaged in laboratory analysis and research of geological materials. These geologists are experts not only in their parent field, but also bring new knowledge in the methodology of analytical chemistry, namely spectrometry. Contributions to the conference program in the field of analysis of geological materials are therefore very much in demand, as well as participants - geologists, who are warmly welcome to this conference.</p>
<p> The microsymposium entitled <strong>&quot;Recent progress in vibrational spectroscopy - Celebrating 50 years of SERS and the 65th anniversary of A. Smekal death&quot;</strong> is the inherent part of the <strong>Conference programme</strong> as a parallel session. Invited as well as short lectures in the Microsymposium will cover a wide range of topics targeted on recent progress in Raman and infrared spectroscopy and their applications. A particular attention will be given to special methods of Raman spectroscopy, namely resonance Raman scattering (RRS) and surface-enhanced/resonance/Raman scattering (SERS and SERRS) as well as to time-resolved IR and Raman spectroscopy. Moreover, the development and application of advanced techniques of vibrational spectroscopis imaging/mapping both in micro- and nanoscale will be covered. Furthermore, application of IR and Raman spectroscopy in various branches of chemistry, material science, geology, gemmology, art conservation, environmental science, food processing, biology and medicine will be an inherent part of the Microsymposium scope.</p>
<p>A series of specialized colloquia entitled <strong>“Mössbauer Spectroscopy in Materials Science (MSMS)”</strong> was initiated in the Central Europe. They have been almost regularly organized since 1994 alternatively in Slovakia and Czech Republic with a two-year periodicity. Firstly, MSMS colloquium represents an important forum for researchers in materials science who share their results on understanding the solid-state matter employing Mössbauer spectroscopy. Secondly, the conference organizers invite several scientists well distinguished in Mössbauer spectroscopy worldwide to give both tutorial lectures and lectures of broad perspective to convey the knowledge to young generation (researcher beginners, PhD students, etc.). Thirdly, cutting-edge works are presented stimulating future studies and research orientation in the field of Mössbauer spectroscopy.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSSC_and_MSMS_2024_Partneri_akce_ffae17979b/CSSC-and-MSMS-2024-Partneri-akce.jpg" alt="CSSC &amp; MSMS 2024: Conference Partners"/></p>
<h3><strong>CONFERENCE TOPICS</strong></h3>
<h4><strong>Czech - Slovak Spectroscopic Conference</strong></h4>
<ul>
<li>Atomic spectrometry (AAS, ICP-MS, AFS, OES, etc.)<ul>
<li><a href="https://icpms.labrulez.com/library?instrumentationItems=85"><strong>AAS</strong></a></li>
<li><a href="https://icpms.labrulez.com/library?instrumentationItems=83"><strong>ICP-MS</strong></a></li>
<li><a href="https://icpms.labrulez.com/library?instrumentationItems=84"><strong>ICP-OES</strong></a></li>
</ul>
</li>
<li>Molecular spectrometry/spectroscopy (UV-Vis, NMR, Raman, IR, etc.)<ul>
<li><a href="https://icpms.labrulez.com/library?instrumentationItems=82"><strong>UV-Vis</strong></a></li>
<li>IČ: <a href="https://icpms.labrulez.com/library?instrumentationItems=89"><strong>FTIR</strong></a>, <a href="https://icpms.cz/library?instrumentationItems=49"><strong>NIR</strong></a></li>
<li><a href="https://icpms.labrulez.com/library?instrumentationItems=48"><strong>Raman</strong></a></li>
</ul>
</li>
<li>XRF spectrometry (EDS, WDS, XRF, PIXE, etc.)</li>
<li>Mass spectrometry (LC-MS, GC-MS, MALDI – MS, DESI-MS, etc.)<ul>
<li><a href="https://gcms.labrulez.com/library?instrumentationItems=3"><strong>GC-MS</strong></a></li>
<li><a href="https://lcms.labrulez.com/library?instrumentationItems=31&amp;search=ESI"><strong>ESI-MS</strong></a></li>
<li><a href="https://lcms.labrulez.com/library?instrumentationItems=25-31&amp;search=ESI"><strong>MALDI</strong></a></li>
<li><a href="https://lcms.labrulez.com/library?instrumentationItems=31&amp;search=DESI"><strong>DESI-MS</strong></a></li>
<li><a href="https://lcms.labrulez.com/library?instrumentationItems=31"><strong>LC-MS</strong></a></li>
</ul>
</li>
<li>Instrumental radioanalytical methods (Gamma spectroscopy, NAA, etc.)</li>
<li>Mössbauer spectroscopy</li>
<li>Laser spectroscopy</li>
<li>Special spectroscopy techniques</li>
<li>Sample preparation and introduction techniques</li>
<li>Applications and metrology</li>
<li>New techniques and data processing methods in IR and Raman spectroscopy</li>
<li>Time-resolved IR and Raman spectroscopy, spectral monitoring</li>
<li>Special methods of Raman spectroscopy: resonance Raman, SERS, SERRS, TERS</li>
<li>IR and Raman spectral mapping and imaging in micro- and nano-scale</li>
<li>Applications of IR and Raman spectroscopy in chemistry, geology, material science</li>
<li>Biological, biophysical, biochemical, medical and environmental applications of IR and Raman spectroscopy</li>
</ul>
<h4><strong>Mössbauer Spectroscopy in Material Science</strong></h4>
<ul>
<li>Environmental, catalytic, biologic, medical applications</li>
<li>Magnetism, magnetic nanoparticles, molecular magnets</li>
<li>Nanomaterials, nanostructures, nanocomposites, core-shell nanoparticles, thin films</li>
<li>Nanomaterials in biomedicine</li>
<li>Nuclear forward scattering in materials science</li>
<li>Solid-state chemistry and physics</li>
<li>Industrial applications, corrosion</li>
<li>Instrumentation, software</li>
<li>Earth Science, Mineralogy and Archaeology</li>
<li>New techniques and data processing methods in IR and Raman spectroscopy</li>
<li>Time-resolved IR and Raman spectroscopy, spectral monitoring</li>
<li>Special methods of Raman spectroscopy: resonance Raman, SERS, SERRS, TERS</li>
<li>IR and Raman spectral mapping and imaging in micro- and nano-scale</li>
<li>Applications of IR and Raman spectroscopy in chemistry, geology, material science</li>
</ul>
<p>-Biological, biophysical, biochemical, medical and environmental applications of IR and Raman spectroscopy</p>
<h3><strong>INVITED SPEAKERS</strong></h3>
<ul>
<li><p><strong>Prof. César Augusto Barrero Meneses</strong> (University of Antioquia, Medellín, Colombia)</p>
</li>
<li><p><strong>Prof. Lothar Brecker</strong> (University of Vienna, Austria)</p>
</li>
<li><p><strong>Prof. Volker Deckert</strong> (Friedrich Schiller University Jena, Germany)</p>
</li>
<li><p><strong>Prof. Stanislaw M. Dubiel</strong> (AGH University of Science and Technology, Krakow, Poland)</p>
</li>
<li><p><strong>Jean-Marc Greneche</strong> (University Le Mans, France)</p>
</li>
<li><p><strong>Prof. Zoltán Homonnay</strong> (Eötvös Loránd University, Budapest, Hungary)</p>
</li>
<li><p><strong>Prof. Antonín Kaňa</strong> (University of Chemistry and Technology Prague, Czech Republic)</p>
</li>
<li><p><strong>Dr. Filip Košek</strong> (Charles University in Praque, Czech Republic)</p>
</li>
<li><p><strong>Dr. Stjepko Krehula</strong> (Ruđer Bošković Institute, Zagreb, Croatia)</p>
</li>
<li><p><strong>Prof. Andreas Limbeck</strong> (Vienna University of Technology, Austria)</p>
</li>
<li><p><strong>Prof. Bernhard Lendl</strong> (Vienna University of Technology, Austria)</p>
</li>
<li><p><strong>Prof. Ruslan Mariychuk</strong> (University of Prešov, Slovakia)</p>
</li>
<li><p><strong>Prof. Jan Preisler</strong> (Masaryk University, Brno, Czech Republic)</p>
</li>
<li><p><strong>Prof. Volker Schünemann</strong> (Technical University of Kaiserslautern, Germany)</p>
</li>
<li><p><strong>Prof. Andrii Vyshnikin</strong> (Pavol Jozef Šafárik University in Košice, Slovakia)</p>
</li>
</ul>
<h3><strong>MEDAL AWARDS</strong></h3>
<p>The Ioannes Marcus Marci Spectroscopic Society (IMMSS) and Slovak Spectroscopic Society (SSS) present awards to eminent personalities in the field of spectroscopy, bearing the names of Ioannes Marcus Marci, eminent scientist, physician and academic dignitary, and Nicolaus Konkoly-Thege, eminent scientist, physicist and astronomer.</p>
<h5><strong>Prof. Ernest Beinrohr</strong></h5>
<ul>
<li>University of St. Cyril and Methodius in Trnava, Slovakia</li>
<li><strong>Ioannes Marcus Marci Medal</strong></li>
</ul>
<h5><strong>Prof. Ewa Bulska</strong></h5>
<ul>
<li>University of Warsaw, Poland</li>
<li><strong>Ioannes Marcus Marci Medal</strong></li>
</ul>
<h5><strong>Prof. Martin Hof</strong></h5>
<ul>
<li>J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic</li>
<li><strong>Ioannes Marcus Marci Medal</strong></li>
</ul>
<h5><strong>Prof. Viktor Kanický</strong></h5>
<ul>
<li>Masaryk University, Brno, Czech Republic</li>
<li><strong>Nicolaus Konkoly-Thege Medal</strong></li>
</ul>
<h3><strong>HISTORY AND TRADITION</strong></h3>
<p>The 18th Czech-Slovak Spectroscopic Conference (18th CSSC) is a proceeding based on a long-term scientific cooperation between Ioannes Marcus Marci Spectroscopic Society and The Slovak Spectroscopic Society. The meetings organized by the former Czechoslovak spectroscopic society (1949 – 1993) in the past and the continuation in the tradition of the national Slovak and Czech spectroscopic conferences now, since 2008, exist as a joint event. </p>
<p>The Czech spectroscopic conference was repeated every four years, while the Slovak spectroscopic conference took place as a biennial. Joint scientific meetings are now held every two years alternately in the Czech Republic and Slovakia. The order may change if this conference is combined with the European Symposium on Analytical Spectrometry (ESAS). To make the tradition of the original separate national conferences clear, the conferences held in the Czech Republic retain their original serial numbering, and the same is true of the conferences held in Slovakia. To recall history, it is possible to take a look at some of the conferences of the past twenty years. </p>
<h3><strong>VENUE</strong></h3>
<p>The conference will take place in Kurdějov, a charming village cradled amidst the rolling vineyards of southern Moravia. Known for its rich viticultural heritage, this quaint destination offers a unique blend of intellectual pursuits, cultural treasures, and breathtaking natural landscapes.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSSC_and_MSMS_2024_venue_Kurdejov_dcea3cb164/CSSC-and-MSMS-2024-venue-Kurdejov.jpg" alt="CSSC &amp; MSMS 2024: the conference will take place in a charming village of Kurdějov"/></p>
<p>The conference takes place in a modern <a href="https://www.hotelkurdejov.cz/"><strong>hotel Kurdějov</strong></a>  providing all amenities, situated right in the heart of Kurdějov. The hotel offers a tranquil retreat, providing a perfect blend of modern comfort and the rustic charm of the countryside. You can enjoy accompanying activities such as bowling, wellness, wine tasting or a tour of the Jan Křtitel church.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSSC_and_MSMS_2024_venue_hotel_Kurdejov_1789faa26c/CSSC-and-MSMS-2024-venue-hotel-Kurdejov.jpg" alt="CSSC &amp; MSMS 2024: Conference venue - hotel Kurdějov"/></p>


18th Czech - Slovak Spectroscopic Conference (CSSC) & Mössbauer Spectroscopy in Materials Science (MSMS) - Registration

CSSC & MSMS 2024 (Conference programme)

Digital Immunoassay for Biomarker Detection Based on Single-Particle Laser Ablation ICP MS

<p><strong>This study presents a novel method for determining transport efficiency (TE) in laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS), based on counting upconversion nanoparticles from gels analyzed by fluorescent microscopy. The approach proved highly effective for a 2940-nm laser ablation system, achieving near-quantitative nanoparticle desorption, with TE largely dependent on ablation cell design and aerosol transport.</strong></p><p>For a 213-nm laser, incomplete desorption and nanoparticle redeposition at low fluences could affect TE reproducibility, while a 193-nm laser caused nanoparticle disintegration, raising baseline noise and lowering sensitivity—rendering it unsuitable for TE measurement. This method demonstrates versatility for TE assessment in LA-ICP-MS, while clearly outlining limitations linked to wavelength and fluence.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c01306"><strong>A Method for Determination of Transport Efficiency in Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Tissue Analysis</strong></a></h4><p>Jaromír Stráník, Vilém Svojanovský, Julie Weisová, Kateřina Uhrová, David Clases, Antonín Hlaváček* and Jan Preisler*</p><p>Anal. Chem. 2025, 97, 25, 12940–12946</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.5c01306">https://doi.org/10.1021/acs.analchem.5c01306</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><p>Inductively coupled plasma–mass spectrometry (ICP-MS) stands at the forefront of analytical techniques, pivotal in advancing our understanding of elemental composition across diverse fields ranging from geological applications to environmental monitoring, as well as biological research. Recognized for its excellent sensitivity and precision, ICP-MS enables the quantification of trace elements with remarkable accuracy and, now more often, the characterization of nanoparticles and microparticles. In this analytical landscape, the measurement of transport efficiency (TE) is crucial. TE, which is defined as the ratio of analyte transported from the matrix to the analyte fraction reaching the detector, is a fundamental parameter for the concentration sensitivity of the ICP-MS experiment.</p><p>Various methods have been developed to determine TE, particularly for nebulizer sample introduction systems. <a href="javascript:void(0);">(1)</a> In single-particle (SP) nebulizer measurements, TE is essential for accurately determining particle number and size. <a href="javascript:void(0);">(2,3)</a> Therefore, with the growth of SP analysis by ICP-MS, techniques analyzing particle frequency or particle size of stable (metallic) nanoparticles have become increasingly popular for TE determination. <a href="javascript:void(0);">(2−4)</a> Additionally, dynamic mass flow method was proposed in 2020, which is based on measuring directly and continuously the weight of sample uptake and the weight of sample reaching the plasma online over time while the ICP-MS system is in equilibrium and was used for the characterization of nanoparticles. <a href="javascript:void(0);">(5)</a></p><p>Determining TE for laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) is less common and more challenging. Aerosol generation and transport in LA-ICP-MS is a complex process that can be influenced by sample morphology, sample composition, laser parameters, carrier gas, particle size and shape, and other factors related to sample atomization and mass spectrometer setup. <a href="javascript:void(0);">(6−9)</a></p><p>Existing methods adapted from solution analysis are not directly applicable. Standard techniques involve capturing aerosols using filters or multilevel impactors, which are often accompanied by an optical particle counter. <a href="javascript:void(0);">(10)</a> This is typically followed by sample weighing before and after the ablation process and characterizing the captured aerosol. <a href="javascript:void(0);">(10−15)</a> With the growing interest in ablating intact nanoparticles in the SP mode, <a href="javascript:void(0);">(16−20)</a> the particle size method has recently found application in laser ablation (LA) systems. <a href="javascript:void(0);">(20)</a> Additionally, TE has been determined by applying a suspension of nanoparticles of a known number concentration to tissue sections. <a href="javascript:void(0);">(21)</a> In our earlier work, we presented a method for TE determination in which we precisely deposited gold nanoparticles using a piezoelectric dispenser on gelatin sections for LA-SP-ICP-MS analysis and silicon wafers for reference scanning electron microscopy. <a href="javascript:void(0);">(19)</a></p><p>However, conventional methods for TE determination have limitations. They often require special equipment and describe the aerosol fate only in the sample introduction system rather than the whole ICP-MS system. Some rely on particle characteristics provided by manufacturers, which are often only approximated, primarily due to an unknown degree of aggregation. Challenges such as nanoparticle suspension aging, pipetting errors, and nanoparticle adsorption during sample preparation underscore the need for a reliable reference method, mainly when precision and accuracy are paramount in TE determination. Recently, nanoparticles embedded in thin polymer films were applied for their characterization and calibration of LA-SP-ICP-MS. <a href="javascript:void(0);">(22)</a></p><p>We present a method for determining transport efficiency in LA-ICP-MS using agarose layers containing photon-upconversion nanoparticles (NPs). These lanthanide-doped nanocrystals absorb infrared light (976-nm) and emit at shorter near-infrared and visible wavelengths in a nonlinear photoluminescence process known as photon-upconversion. This unique property enables background-free imaging and accurate single-nanoparticle counting via photon-upconversion microscopy, serving as a reference method. The method is compatible with laser wavelengths (2940, 213, and partially 193-nm), providing robust TE evaluation in LA-SP-ICP-MS systems.</p><h4><strong>Experimental Section</strong></h4><h5><strong>ICP-MS Data Evaluation</strong></h5><p>Detailed information on the quadrupole and TOF ICP-MS setups, data acquisition and processing, including correction of coincidence of multiple NPs is available in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c01306/suppl_file/ac5c01306_si_001.pdf">Supporting Information</a>. The TE was calculated based on the ratio of the number of NPs detected by UCM and LA-SP-ICP-MS.</p><p>An <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=ICP-MS+7900"><strong>ICP quadrupole mass spectrometer Agilent 7900</strong></a> (Agilent Technologies, Santa Clara, CA) was combined with 2940, 213, and 193-nm laser ablation systems. The 193-nm laser ablation system was also coupled to the Vitesse ICP-TOF mass spectrometer (Nu Instruments, UK).</p><p>The 2940-nm laser ablation system was linked to the quadrupole ICP mass spectrometer through a 1.34-mm inner diameter stainless steel capillary transport tube (Swagelok, Solon, OH) via a low-volume laser ablation adapter from Glass Expansion (Port Melbourne, Australia) with a make-up gas Ar flow rate of 0.4 L/min to introduce the dry aerosol into the ICP. A quartz plasma torch with a 2.5-mm injector width (AHF analysentechnik AG, Tübingen, Germany) was employed to sustain a total gas flow rate of 2.0 L/min. The ICP-MS was operated in a time-resolved analysis mode with the shortest dwell time possible, 100 μs, to minimize errors from partial NP event integration and coincidental events. The isotope of choice detected within the NPs was 89Y, as it had the highest abundance. We used yttrium standard solution (Analytika, Prague, Czech Republic) diluted to 1 μg/L to adjust the position of the plasma torch and voltage of the ion optics and maximize the detector's response at the mass-to-charge ratio (m/z) 89.</p><p>For the 213-nm LA, the same MS setup was used as in the 2940-nm LA-ICP-MS, with a minor change to tubing and Ar flow rate. The connection between the ablation cell and the plasma torch was facilitated by a 2.5-mm inner diameter polyurethane tube (Parker Legris, Cleveland, OH), and the 1.1 L/min Ar make-up gas was introduced via a quick-connect.</p><p>For the 193-nm LA, the quadrupole system was connected to the laser using a Fluorinated Ethylene Propylene (FEP) tube with an inner diameter of 2 mm. Gas flow rates in the ablation cell were configured to match those used for the 213-nm laser ablation system. The quadrupole mass spectrometer settings were identical to those used for the 213-nm measurements. In the case of LA-ICP-TOFMS, the laser was coupled to the mass spectrometer via an aerosol rapid introduction system (ARIS) for aerosol transport. Parameters such as Ar flow, torch position, and TOF parameters were optimized daily to obtain the best sensitivity by ablating the NIST 612 "Trace Elements in Glass" reference material to maximize the response for 115In, 238U, and 89Y. Final measurements were conducted over a reduced mass range of 39–89 amu with a 76.89 μs dwell time.</p><h4><strong>Results and Discussion</strong></h4><p>Two approaches were examined to determine transport efficiency. In the “Random area” approach, square areas imaged by UCM and LA-SP-ICP-MS were chosen randomly on the same glass slide with the agarose layer. In the “Grid area” approach, an identical square area defined by the grid generated in the agarose layer was inspected by UCM and LA-SP-ICP-MS. Three lasers, with wavelengths of 2940, 213, and 193-nm, were used for ablation. Applicability of agarose as a standard for TE determination of soft tissues was confirmed by comparing numbers of NPs desorbed from agarose and mouse brain tissue sections. Details are available in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c01306/suppl_file/ac5c01306_si_001.pdf">Supporting Information (Figure S2)</a>.</p><h5><strong>2940-nm LA-ICP-MSI</strong></h5><p>For imaging, a square in the grid-modified agarose layers was ablated by the IR system under conditions adjusted for imaging; the time between two consecutive laser pulses was set to ∼100 ms, above the ablation cell washout time of ∼75 ms, <a href="javascript:void(0);">(19)</a> to minimize the overflow of NPs into the following pixels. For 213-nm ablation, conditions remained the same as in the case of the random area approach.</p><p>Overlaying images with different lateral resolutions, shifts, and rotations with no distinctive features is not straightforward and was accomplished using a special algorithm, as detailed in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c01306/suppl_file/ac5c01306_si_001.pdf">Figure S6 of the Supporting Information</a>. UCM and 2940-nm LA-SP-ICP-MS maps of a selected square area on the agarose layer are shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c01306?ref=utm_source=pubsw&amp;utm_medium=web&amp;utm_campaign=IC004_ST0004D_T000549_OA_Microsite_LP&amp;src=IC004_ST0004D_T000549_OA_Microsite_LP#fig1">Figure 1</a>. While at the macroscopic level, the images show fine alignment, careful examination reveals an imperfect overlay of UCM-localized NPs with the ICP-MS map. This misalignment can be attributed to NP spillover into adjacent pixels, mostly due to mechanical limitations of the <i>XY</i> stage discussed previously. Another possible contribution may be the accumulation of material from the previous line on the edge of the next line; this is also seen on the square edges in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c01306?ref=utm_source=pubsw&amp;utm_medium=web&amp;utm_campaign=IC004_ST0004D_T000549_OA_Microsite_LP&amp;src=IC004_ST0004D_T000549_OA_Microsite_LP#fig1">Figure 1</a>A. Nevertheless, the spillover of NPs into surrounding pixels does not affect the total number of detected NPs, only their spatial distribution; therefore, the agarose layers can still be used for accurate TE determination. The average detected NP counts for IR-LA and the calculated TE are in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c01306?ref=utm_source=pubsw&amp;utm_medium=web&amp;utm_campaign=IC004_ST0004D_T000549_OA_Microsite_LP&amp;src=IC004_ST0004D_T000549_OA_Microsite_LP#tbl1">Table 1</a>, NP counts from individual experiments can be found in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c01306/suppl_file/ac5c01306_si_001.pdf">Supporting Information</a>. By ablating a defined area with a precisely known number of NPs, we eliminated the effect of inhomogeneous NP dispersion in the gel and thus reduced the RSD’s of the method by 3% or 5% for 2940- or 213-nm laser ablation, respectively. The TE value was in excellent agreement with that determined by the random area approach.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_25_12940_12946_Figure_1_2940_nm_LA_SP_ICP_MS_and_UCM_image_overlay_95400830df.jpg" alt="Anal. Chem. 2025, 97, 25, 12940–12946: Figure 1. 2940-nm LA-SP-ICP-MS and UCM image overlay. (A) UCM image, with bright spots indicating individual NP signals. (B) Digital map of NP distribution measured by 2940-nm LA-SP-ICP-MSI, displayed in grayscale. The shades of gray represent the precise number of NPs in the agarose layer, with a pixel size of 15 µm × 15 µm. (C) Overlay of images A and B, where NPs localized by UCM are highlighted as green circles. (D) A zoomed-in section of panel (C), providing a detailed look at the overlay." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_25_12940_12946_Figure_1_2940_nm_LA_SP_ICP_MS_and_UCM_image_overlay_95400830df.jpg 161w," sizes="100vw" width="1600" height="1557"></p><h4><strong>Conclusion</strong></h4><p>This study presents a direct method for determining TE in LA-SP-ICP-MS. The TE is calculated from the numbers of NPs released from agarose layers, which were previously characterized by UCM. The agarose layers with a specific number of NPs can be prepared, characterized, and archived for future use as a storage-stable standard. This study provides valuable insights into the laser ablation of Y-based upconversion NPs, highlighting wavelength-specific influence on their integrity and transport. The observed differences in NP integrity and transport reflect the behavior of these particular NPs in the employed laser ablation systems and may not directly apply to other NP types and sizes.</p><p>With the 2940-nm laser, the method was robust and reliable, achieving near-quantitative NP desorption. Yet, reablation is advisible to confirm the proper adjustment of the laser ablation system. The TE for this wavelength was primarily determined by the design of the ablation cell and the efficiency of aerosol transport. The 213-nm laser, while effective, required careful optimization of fluence to mitigate incomplete desorption and possible NP redeposition, which introduced variability in TE measurements. In contrast, the 193-nm laser showed significant limitations due to NP disintegration, elevated baseline, and noise, leading to a reduced TE. A possible remedy to NP disintegration could be using a poly(methyl methacrylate) or poly(vinylpyrrolidone) matrix for NP immobilization, <a href="javascript:void(0);">(22)</a> probably due to stronger absorption at 193-nm. We also tested the application of a TOF instrument, which potentially could miss small particles due to its reduced duty cycle. However, for NP sizes used here, we could demonstrate comparable TEs. This might be interesting for future studies as LA-SP-ICP-TOFMS would allow significantly faster mapping and a multiplex analysis, which are critical factors for many biochemical and/or medical applications (e.g., imaging mass cytometry).</p><p>This work emphasizes tailoring TE assessment methods to the unique characteristics of each LA system. By addressing challenges such as particle loss and signal variability, this method lays foundations for improved NP characterization and LA imaging across diverse applications. Future research should focus on optimization of this approach for systems operating at shorter wavelengths, which are the most used in the ICP-MS applications nowadays. Additionally, strategies to minimize particle loss and improve signal processing will be crucial for optimizing TE and broadening the applicability of this method.</p>

A Method for Determination of Transport Efficiency in Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Tissue Analysis

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