<p><strong>Jiří Nováček, a researcher and the deputy director for research infrastructure at CEITEC Masaryk University, shares insights into his journey with Brnoregion Microscopy. Sparked by a conversation with Jan Neuman, Nováček saw the potential of integrating Atomic Force Microscopy (AFM) into life-science electron microscopy. Today, he plays an active role in the community, collaborating with colleagues to enhance educational initiatives.&nbsp;</strong></p><h4><strong>How did you become involved with Brnoregion Microscopy?</strong></h4><p>My involvement with Brnoregion Microscopy began through my interaction with <a target="_blank" rel="noopener noreferrer" href="https://brnoregion.com/en/news/people-of-brnoregion-microscopy-jan-neuman-nenovis">Jan Neuman</a>. We were discussing the potential applications of their integrated Atomic Force Microscopy (AFM) module in the field of life-science electron microscopy. I was immediately intrigued by the idea of forming a microscopy community and saw great potential in it. Jan's vision of fostering stronger local connections among colleagues with complementary expertise resonated with me. This alignment of interests and the shared goal of advancing our understanding and capabilities in microscopy made me eager to join and contribute to the community.&nbsp;</p><h4><strong>What role do you play in the community?</strong></h4><p>Currently, I am an active member of the Community working group within Brnoregion Microscopy. In this role, I collaborate closely with Tomáš Vystavěl from Thermo Fischer Scientific on various educational initiatives. Our focus is on enhancing the educational aspects of the Microscopy community, looking for opportunities for deepening the knowledge and skills in the area of microscopy in our region. It's a rewarding experience to be part of a team that is dedicated to fostering learning and innovation in this field.&nbsp;</p><h4><strong>What benefits does it bring to you?</strong></h4><p>Being part of the Microscopy community has been incredibly enriching for me. It provides a platform for continuous learning and exposure to the latest advancements in microscopy technology and techniques. Working together stimulates new ideas and approaches, which are key to our ongoing development. Additionally, it offers the opportunity to network with experts and peers, opening doors to potential collaborations and projects. That’s how it works in our local innovation ecosystem… &nbsp;</p><h4><strong>Do you have any message for the community?</strong></h4><p>Whether you're already involved or just curious, join the upcoming <a target="_blank" rel="noopener noreferrer" href="https://brnoregion.com/en/events/120-seconds-meet-brnoregion-microscopy-24-11">120 seconds: Meet Brnoregion Microscopy</a> event. It's a fantastic opportunity to see first-hand how the Microscopy community can benefit you. These events are designed to showcase the practical applications of microscopy and the innovative work being done by our members.&nbsp;</p>

People of Brnoregion Microscopy: Jiří Nováček (CEITEC MUNI)

<p><strong>In the week from January 20, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Anton Paar: Fundamentals of Scratch</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4449">Registration</a></div></li></ul></div><p>This webinar provides a thorough introduction to the theory and practice of instrumented scratch testing, a critical method for evaluating the mechanical properties of materials under stress.</p></div><h5><strong>2.&nbsp;Mettler-Toledo: Advanced Inspection of Canned Goods with X-ray Inspection</strong></h5><div><div><ul><li><div>We, 22.1.2025 09:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4658">Registration</a></div></li></ul></div><p>Join our webinar to discover the wide range of benefits that x-ray inspection offers canned applications - including advanced detection capabilities and integrity and completeness checks.</p></div><h5><strong>3.&nbsp;Bruker: Nanoscale Chemical Characterization of Semiconductor Materials and Devices using Photothermal AFM-IR</strong></h5><div><div><ul><li><div>We, 22.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4681">Registration</a></div></li></ul></div><p>In this webinar, Bruker nanoscale IR spectroscopy experts show how photothermal AFM-IR can identify the local chemistry of defects.</p></div><h5><strong>4.&nbsp;Agilent Technologies: Using LDIR for Advanced Microplastics Characterization</strong></h5><div><div><ul><li><div>Th, 23.1.2025 09:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4683">Registration</a></div></li></ul></div><p>This session will highlight the advanced features of LDIR technology and its critical applications in analyzing microplastics within various matrices.</p></div><h5><strong>5.&nbsp;LECO: Optimization of Combustion Methods for Determination of Low-Level Nitrogen-Protein in Food</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4686">Registration</a></div></li></ul></div><p>In this webinar, Lloyd Allen, Ph.D., LECO's Director of the Technical Services Laboratory, will share practical insights on overcoming challenges using advanced combustion instruments.</p></div><h5><strong>6.&nbsp;Agilent Technologies: Different Cones / Nebulizers / Sample Introduction Systems .. Where and When to Use them</strong></h5><div><div><ul><li><div>Th, 23.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4546">Registration</a></div></li></ul></div><p>Different Cones / Nebulizers / Sample Introduction Systems .. Where and When to Use them</p></div>

Webinars LabRulezICPMS Week 4/2025

<p><strong>The goal of this study is to advance molecular epidemiology by integrating and analyzing large-scale population data from two complementary platforms: quantitative NMR spectroscopy for 209 lipoprotein measures and LC-MS/MS for 809 lipid classes and species. Together, these datasets encompass 1018 molecular measures across 7830 participants from two population cohorts. The study aims to elucidate the molecular and compositional relationships within the circulatory lipid transport system and their connections to physiological and clinical characteristics.</strong></p><p>Through cluster analyses and unsupervised neural network techniques (self-organizing maps), the study identifies inherent links between deep molecular data and population health traits, including cardiometabolic risk factors such as lipoprotein lipids, apolipoprotein B, ceramides, and oxidized lipids. Notable findings include the consistent cardiometabolic risk associations of triglyceride species across the lipoprotein cascade and the intricate metabolic profiles of cholesteryl ester species, which vary in their risk implications. This work highlights the unprecedented detail and compatibility of combined omics methodologies, paving the way for comprehensive molecular insights and new opportunities in large-scale population health studies.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229"><strong>Personalized Profiling of Lipoprotein and Lipid Metabolism Based on 1018 Measures from Combined Quantitative NMR and LC-MS/MS Platforms</strong></a></h4><p>Siyu Zhao, Corey Giles, Kevin Huynh, Johannes Kettunen, Marjo-Riitta Järvelin, Mika Kähönen, Jorma Viikari, Terho Lehtimäki, Olli T. Raitakari, Peter J. Meikle, Ville-Petteri Mäkinen, Mika Ala-Korpela</p><p>Analytical Chemistry, Vol 96/Issue 52</p><p><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229">https://doi.org/10.1021/acs.analchem.4c03229</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p>&nbsp;</p><p class="cke-user_style-important"><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><h3><strong>Abstract</strong></h3><p>Applications of advanced omics methodologies are increasingly popular in biomedicine. However, large-scale studies aiming at clinical translation are typically siloed to single technologies. Here, we present the first comprehensive large-scale population data combining 209 lipoprotein measures from a quantitative NMR spectroscopy platform and 809 lipid classes and species from a quantitative LC-MS/MS platform. These data with 1018 molecular measures were analyzed in two population cohorts totaling 7830 participants. The association and cluster analyses revealed excellent coherence between the methodologically independent data domains and confirmed their quantitative compatibility and suitability for large-scale studies. The analyses elucidated the detailed molecular characteristics of the heterogeneous circulatory macromolecular lipid transport system and the underlying structural and compositional relationships. Unsupervised neural network analysis─the so-called self-organizing maps (SOMs)─revealed that these deep molecular and metabolic data are inherently related to key physiological and clinical population characteristics. The data-driven population subgroups uncovered marked differences in the population distribution of multiple cardiometabolic risk factors. These include, e.g., multiple lipoprotein lipids, apolipoprotein B, ceramides, and oxidized lipids. All 79 structurally unique triglyceride species showed similar associations over the entire lipoprotein cascade and indicated systematically increased risk for carotid intima media thickening and other atherosclerosis risk factors, including obesity and inflammation. The metabolic attributes for 27 individual cholesteryl ester species, which formed six distinct clusters, were more intricate with associations both with higher─e.g., CE(16:1)─and lower─e.g., CE(20:4)─cardiometabolic risk. The molecular details provided by these combined data are unprecedented for molecular epidemiology and demonstrate a new potential avenue for population studies.</p><p>Lipoprotein lipids have had a central role in clinical risk assessment for cardiometabolic diseases since the 1950s. <a href="javascript:void(0);" aria-label="reference 1">(1)</a> The realization that <sup>1</sup>H NMR spectroscopy would be inherently suited for lipoprotein analytics took place in the early 1990s. <a href="javascript:void(0);" aria-label="reference 2">(2)</a> In addition to the fundamental advantages of <sup>1</sup>H NMR spectroscopy in producing quantitative data, the spherical monolayered structure of lipoprotein particles provides a compelling attribute that the chemical shifts of lipid molecules transported by these particles are dependent on the particle diameter. This phenomenon arises from the orientational order of the surface lipids and the resulting anisotropy of the magnetic susceptibility, and leads to distinctive particle size-dependent frequency shifts. This facilitates comprehensive lipoprotein analytics that is pertinent to the metabolic constituents of the lipoprotein cascade. Practical applications and statistical considerations have indicated that 14 lipoprotein subclasses and their main lipid constituents can be robustly quantified. <a href="javascript:void(0);" aria-label="reference 3 and 4">(3,4)</a> Multiple commercial methodologies have also been developed. <a href="javascript:void(0);" aria-label="reference 5 to 7">(5−7)</a> Here we applied a platform that has been widely applied in large-scale epidemiology and genetics over the last 15 years with some 500 publications and 2 M samples analyzed, including all the 0.5 M serum samples in the UK Biobank. <a href="javascript:void(0);" aria-label="reference 3, 5 and 8">(3,5,8)</a> This particular platform has also played a key role in yielding open access genetic data, with the most recent genome-wide association study meta-analysis being carried out in 136,000 individuals. <a href="javascript:void(0);" aria-label="reference 9">(9)</a></p><p>Mass spectrometry based lipidomics methodologies have been developed independent of the above and are technologically more challenging than the applications of <sup>1</sup>H NMR spectroscopy. <a href="javascript:void(0);" aria-label="reference 10 to 12">(10−12)</a> Nevertheless, epidemiological applications have become feasible over the recent years and clinical translation has been suggested, for example, with respect to assessing cardiometabolic risk <a href="javascript:void(0);" aria-label="reference 13 and 14">(13,14)</a> and cognitive impairment. <a href="javascript:void(0);" aria-label="reference 15">(15)</a> Initial genetic studies have also been published. <a href="javascript:void(0);" aria-label="reference 16 and 17">(16,17)</a></p><p>The NMR spectroscopy and MS lipidomics approaches to lipid characterization and quantification are fundamentally different (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>). In NMR the serum sample is directly analyzed with the lipoprotein particles intact; this allows quantification of the comprehensive and specific data on the lipoprotein subclasses and lipids. <a href="javascript:void(0);" aria-label="reference 18">(18)</a> Unlike NMR, LC-MS/MS lipidomics calls for lipid extraction of the serum samples as an integral prerequisite for the analyses. <a href="javascript:void(0);" aria-label="reference 19">(19)</a> The lipidomics sample preparations thus represent pooled mixtures of all the lipid molecules from all the circulating lipoprotein particles. All the information from the specific lipoprotein origin of a certain lipid in the circulation is lost in the extraction phase. However, with LC-MS/MS hundreds of unique lipid species can be analyzed from dozens of different molecular lipid classes. <a href="javascript:void(0);" aria-label="reference 12 and 20">(12,20)</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2024_96_52_20362_20370_Figure_1_Schematic_illustration_of_the_methodological_differences_and_analytical_characteristics_between_1_H_NMR_and_LC_MSMS_lipidomics_and_their_molecular_views_into_serum_b9907a5e30.jpeg" alt="Anal. Chem. 2024, 96, 52, 20362-20370: Figure 1. Schematic illustration of the methodological differences and analytical characteristics between 1H NMR and LC-MS/MS lipidomics and their molecular views into serum lipids. (A) The lipoprotein analysis with NMR is performed directly from the intact serum sample. (B) For the LC-MS/MS analysis all the lipids need to be extracted to a homogeneous lipid-soluble mixture. This allows LC-MS/MS to reach detailed molecular quantification, however, as a total serum concentration without any information on the originating lipoproteins. (C,D) The NMR analysis gives molecular information on 14 lipoprotein subclasses with the abundant core and surface lipids quantified. The percentages depict the compositional variation of these lipids in different lipoprotein particles." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2024_96_52_20362_20370_Figure_1_Schematic_illustration_of_the_methodological_differences_and_analytical_characteristics_between_1_H_NMR_and_LC_MSMS_lipidomics_and_their_molecular_views_into_serum_b9907a5e30.jpeg 192w," sizes="100vw" width="947" height="771"></p><p>Lipoprotein particles are the sole transport vehicles for all lipid molecules in serum, <a href="javascript:void(0);" aria-label="reference 18, 21 and 22">(18,21,22)</a> though albumin contains the bulk of free fatty acids and acylcarnitines, and transports also various lyso-type of lipid molecules. <a href="javascript:void(0);" aria-label="reference 23 and 24">(23,24)</a> The NMR and the LC-MS/MS data on serum essentially represent the same lipids as noted by Ghorasaini et al. <a href="javascript:void(0);" aria-label="reference 25">(25)</a> The NMR provides the size-resolution and metabolic information on lipoprotein particles (209 measures) and the LC-MS/MS lipidomics opens up a prolific and detailed molecular view on all the circulating lipids (809 measures).</p><p>These different methodologies and molecular views to study serum lipids have independently advanced toward large-scale population studies. Here, we integrate these state-of-the-art data─over 1000 quantitative molecular lipid measures per individual─in two large-scale population cohorts of over 7800 participants. These data reveal the fundamental relationships between the lipoprotein subclass cascade and metabolism (NMR) and the plethora of specific molecular information (LC-MS/MS). The molecular and metabolic corollaries are numerous, and we elaborate on some pertinent findings. Among the various analyses we also demonstrate the admirable coherence within the independent experiments and data domains. It is also uncovered─via data-driven, unsupervised neural network analysis─that these molecular lipid measures per se can be utilized for metabolically and clinically meaningful subgrouping of populations. The results illustrate that these combined comprehensive data lead to improved molecular characterization of population-level metabolic health and cardiometabolic risk.</p><h3><strong>Materials and Methods</strong></h3><h5><strong><sup>1</sup>H NMR Spectroscopy</strong></h5><p>We applied an NMR platform that has been widely used in epidemiology and for which the general methodological issues have been published. <a href="javascript:void(0);" aria-label="reference 3, 5, 8, 9 and 18">(3,5,8,9,18)</a> The separation of lipoprotein subclasses by proton NMR spectroscopy is based on particle size, <a href="javascript:void(0);" aria-label="reference 2">(2)</a> the platform resolution being 14 subclasses (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c03229#fig2" data-tab="pane-pcw-Figures" id="">Figure 2</a>). <a href="javascript:void(0);" aria-label="reference 3 and 18">(3,18)</a> Independent verification for the number of subclasses has been published by Mihaleva and co-workers with an in-depth handling of statistical grounds. <a href="javascript:void(0);" aria-label="reference 4">(4)</a> Particle (P), phospholipid (PL), free cholesterol (FC), cholesteryl ester (CE), and triglyceride (TG) concentrations are quantified for all 14 subclasses, also allowing calculation of the relative lipid composition for each lipoprotein subclass. The platform also provides all key clinically used lipid measures (e.g., total TG, cholesterol, and HDL-C) as well as apolipoprotein B (apoB) and A-I (apoA-I) concentrations. In total 209 lipoprotein-related measures were analyzed (98 concentration and 70 compositional measures from 14 lipoprotein subclasses, 19 clinical lipid measures, and 22 measures related to fatty acids, average particle sizes, and apoA-I and apoB).</p><h5><strong>LC-MS/MS Mass Spectrometry</strong></h5><p>Serum lipids were extracted as previously described. <a href="javascript:void(0);" aria-label="reference 19">(19)</a> The LC-MS/MS profiling was performed using the standardized methodology described recently by Huynh et al. <a href="javascript:void(0);" aria-label="reference 12">(12)</a> and updated with a larger set of targets. The mass spectrometric details and parameters are given in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c03229/suppl_file/ac4c03229_si_001.pdf">Supporting Information</a>.</p><h3><strong>Conclusions</strong></h3><p>The presented combination of lipoprotein and lipid data from NMR spectroscopy and LC-MS/MS lipidomics led to an unprecedented metabolic and molecular detail with over 1000 measures per person in a population setting of over 7800 individuals. The analyses demonstrated notable coherence between the data from inherently different spectroscopic techniques. The data-driven analyses revealed multiple confirmatory and novel molecular results in relation to systemic lipid metabolism and illustrated the power of all-inclusive lipid data in understanding cardiometabolic population health, related clinical factors, and disease risk. The analyses emphasized the augmented value of interpreting lipidomics data (deep molecular phenotyping) together with lipoprotein data (deep metabolic phenotyping) and association heatmaps between all the lipidomics measures and the key measures characterizing lipoprotein subclass metabolism are provided. These unique motifs are useful as guidelines for the key lipoprotein mediators for various lipidomics associations in biomedical studies when comprehensive lipoprotein profiles are not available.</p>

Personalized Profiling of Lipoprotein and Lipid Metabolism Based on 1018 Measures from Combined Quantitative NMR and LC-MS/MS Platforms

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

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<h4>Unraveling Epigenetic Changes in Cancer Cells</h4>
<p><strong>During this webinar, guest speaker Dr. Babak Faryabi, University of Pennsylvania, discusses utilizing Oligopaint and super-resolution microscopy to investigate the role of transcription hubs in altering genome folding.</strong></p>
<p>Attendees can expect to learn about the power of super-resolution microscopy and chromatin tracing in understanding the three-dimensional structure of the genome in single cells.</p>
<p>The importance of epigenetic changes in cancer pathogenesis has become increasingly clear, yet the underlying mechanisms remain obscure. Our research focuses on identifying epigenetic changes that can render cancer cells addicted to specific transcriptional regulators. I will present evidence that oncogenic subversion of transcription factors leads to disruption of chromosome topology and instruct enhancer elements to aberrantly interact and activate intact key proto-oncogenes. These aberrant epigenetic states can drive cancer cell growth and proliferation in the absence of genetic mutations and may also confer drug resistance. I will also discuss our efforts to develop single-cell molecular tools and experimental models to characterize epigenetic mechanisms of oncogenesis and attendant therapeutic opportunities.</p>
<blockquote>
<p><strong>Presenter:</strong> Babak Faryabi, Ph.D. (Associate Professor of Pathology and Cancer Biology, University of Pennsylvania Perelman School of Medicine)</p>
</blockquote>
<blockquote>
<p>Dr. Faryabi has a distinguished history of research and accomplishments in cancer epigenomics. His work has significantly advanced our understanding of how transcription factors alter cancer genome folding. By conducting the first genome-wide and single-cell resolution measurement of genome looping in triple-negative breast cancer (TNBC) and mantle cell lymphoma (MCL), he discovered that Notch can regulate gene expression by changing genome folding and bringing together distant enhancer and promoter elements on the linear genome. This identified a novel mechanism by which oncogenic Notch alters transcription. Furthermore, he uncovered that Notch-dependent DNA loops bring multiple genomic elements into highly interacting spatial hubs of enhancers and promoters. He is among the first to elucidate the contribution of genome topology organization to cancer therapy resistance. His work revealed the role of genome folding reorganization as a novel epigenetic adaptation mechanism, conferring resistance to Notch inhibitors in T-cell leukemia and to BTK inhibitors in B cell lymphoma. To achieve his central scientific objective, he also innovates machine learning tools and software to better dissect the complexity and heterogeneity of genome structures and regulation in cancer.</p>
</blockquote>
<blockquote>
<p>As the faculty lead at the Center for Personalized Diagnostics at the Hospital of the University of Pennsylvania, his clinical expertise lies in developing genomic assays for diagnostic and predictive markers in cancer.</p>
</blockquote>


Bruker: Notch-Mutated Cancer Genomes in 3D

Notch-Mutated Cancer Genomes in 3D

Find out what super-resolution microscopy can reveal about epigenetic changes in cancer pathogenesis.

<p><strong>Adeno-associated vectors (AAVs) are transforming therapeutics – from gene therapy to vaccines.</strong></p>
<p><strong>A major challenge when developing AAV-based therapeutics is ensuring that empty AAVs that lack therapeutic DNA are removed, increasing the efficacy of the product.</strong></p>
<p>But the empty AAVs are chemically identical to their full counterparts, making this process complicated. Enter mass photometry, a new bioanalytical technique that can efficiently characterize AAVs and distinguish between full and empty AAVs.</p>
<p>This webinar explores how mass photometry can circumvent challenges associated with AAVs, helping to accelerate therapeutic development and manufacturing.</p>
<p>Attend this webinar to: </p>
<ul>
<li><strong>Discover how mass photometry is currently being used in industry</strong></li>
<li><strong>Learn how mass photometry can accurately measure empty/full AAV capsid ratios</strong></li>
<li><strong>Explore how to integrate mass photometry in a GMP-regulated environment</strong></li>
</ul>
<p><strong>Will I receive a certificate of attendance?</strong></p>
<p>Yes, when attending the webinar, you will be able to download a certificate of attendance from the literature section.</p>
<p><strong>Will the webinar be available to watch On-Demand?</strong></p>
<p>Yes, after the live date the recording will be available to watch. Once registered, you will receive an email notifying you when the recording is available along with your on-demand link.</p>
<ul>
<li><strong>Presenter:</strong> Dr. Matt Ranaghan (Senior Applications Scientist, Refeyn)</li>
</ul>


Technology Networks: Enhancing the Development of AAV-Based Therapeutics With Mass Photometry

Enhancing the Development of AAV-Based Therapeutics With Mass Photometry

Mass photometry, a new bioanalytical technique that can efficiently characterize AAVs and distinguish between full and empty AAVs.

Development of AAV-Based Therapeutics With Mass Photometry

<p><strong>Aquaporin (AQP) water channels facilitate water diffusion across lipid bilayers following an osmotic gradient. AQPs are especially important in renal water reabsorption. AQP2 in the renal collecting duct mediates fine-tuning of urine volume in response to the antidiuretic hormone arginine vasopressin (AVP).</strong></p>
<p><strong>AVP mediates plasma membrane insertion of AQP2 from an intracellular reservoir of AQP2 containing vesicles, which instantly increases water reabsorption and urine concentration. The vesicle population cannot be resolved via conventional fluorescent microscopy, therefore detailed information regarding the AQP2 vesicular population is still lacking.</strong></p>
<p>Expansion microscopy (ExM) addresses challenges in resolving vesicle populations. Newly established 4.5x ExM can increase resolution to 60–70 nm. In this webinar, Dr. Lene Niemann Nejsum, Professor of Epithelial Physiology and Pathophysiology at Aarhus University, will present results showing that 4.5 ExM is an ideal approach to obtain detailed information regarding the AQP2 vesicle population. Using 4.5x ExM, spinning disk microscopy and image processing, Dr. Nejsum and her team detected AQP2 vesicles/endosomes. Using different markers of the endosomal system, they obtained detailed information of the cellular AQP2 shuttling itinerary.</p>
<p><strong>Key learning objectives</strong></p>
<ul>
<li>Understand how Aquaporin mediated water transport across lipid bilayers</li>
<li>Gain insight into Studies of intracellular vesicles using Expansion Microscopy</li>
<li>Learn more about the Use of Andor BC43 with Deconvolution for ExM</li>
</ul>
<p><strong>Who should attend?</strong></p>
<p>Both new and experienced Imaris and BC43 users interested in accelerating microscopy projects. The research is relevant across life science projects where visualizsation and analysis is required.</p>
<p><strong>Certificate of attendance</strong></p>
<p>All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p>
<blockquote>
<p><strong>Presenter:</strong> Geraint Wilde (Business Manager - Life Science Microscopy, Oxford Instruments Andor)</p>
</blockquote>
<blockquote>
<p>Dr. Geraint Wilde attained a Ph.D. in Neuroscience in 1997 from the University of Southampton, UK, before holding a number of research positions. He focused on intracellular signalling and gene expression through live-cell imaging. Dr. Wilde eventually pursued a commercial career in microscopy and he joined Andor Technology in 2009, where he is now Product Manager for Microscopy Solutions.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Lene Niemann Nejsum (Professor of Epithelial Physiology and Pathophysiology, Department of Clinical Medicine, Aarhus University)</p>
</blockquote>
<blockquote>
<p>Dr. Lene Niemann Nejsum is a Professor of Epithelial Physiology and Pathophysiology at Aarhus University, Denmark. She earned her PhD in Medicine in 2004, followed by a five-year postdoctoral position at Stanford University, and completed a Doctor medicinae in 2023 from Aarhus University. Her research focuses on epithelial function and aquaporin (AQP) water channels. Her lab investigates the AQP2 vesicle population in renal cells using advanced microscopy, achieving the first visualization of their 3D network. Additionally, her lab has discovered novel AQP functions in cellular morphology, adhesion, migration, and polarity—key mechanisms in cancer development and progression.</p>
</blockquote>


SelectScience: The Cellular Itinerary of Renal Aquaporin-2 Shuttling with 4.5x Expansion Microscopy

The Cellular Itinerary of Renal Aquaporin-2 Shuttling with 4.5x Expansion Microscopy

Results showing that newly established 4.5 expansion microscopy is an ideal approach to obtain detailed information regarding the AQP2 vesicle population.

Renal Aquaporin-2 Shuttling with 4.5x Expansion Microscopy

<p><strong>Join us for an exclusive one-day hybrid event, here we&#39;ll explore the latest trends and innovations in microplastics analysis. The morning session will be live-streamed and recorded, allowing virtual  articipation for those unable to attend in person.</strong></p>
<p>We are excited to invite you to a special <strong>hybrid event</strong> in partnership with <strong>i3 Membrane</strong>, where we will delve into the latest trends and innovations in microplastics analysis. Whether you join us in person or virtually, you’ll gain invaluable insights and hands-on experience.</p>
<p><strong>Event also available in live stream</strong></p>
<h3><strong>Agenda:</strong></h3>
<ul>
<li><p><strong>Morning session:</strong> Gain key insights into <a href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=microplastics+analysis"><strong>microplastics analysis</strong></a>, as well as <a href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=PFAS"><strong>PFAS</strong></a> and other environmental contaminants, through engaging presentations by top researchers and industry experts.</p>
</li>
<li><p><strong>Afternoon session:</strong> Participate in interactive workshops designed to enhance your practical skills in sample preparation. This provides a unique opportunity to master advanced techniques and methodologies under the guidance of professionals. Attend a live demo on the IR QCL-based <a href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=8700+LDIR"><strong>8700 LDIR</strong></a>. Additionally, enjoy a guided laboratory tour offering an exclusive glimpse into the state-of-the-art facilities at the HEREON campus.</p>
</li>
</ul>
<h5>9:15 a.m. - Welcome</h5>
<h5>9:20 a.m. - <strong>From Macroplastics to Mesoplastics: Challenges and Solutions</strong></h5>
<ul>
<li>Dr. Marion Börger, Agilent Technologies,</li>
<li>Dr. Andreas Kerstan, Agilent Technologies</li>
</ul>
<h5>9:40 a.m. - <strong>i3 Membrane Session: Filter Membranes/Packaging - Jacobs</strong></h5>
<ul>
<li>Kathrin Hahn, i3 Membrane</li>
</ul>
<h5>10:00 a.m. - <strong>Exploring Microplastic Sources and Distribution in Australian Soils</strong></h5>
<ul>
<li>Subharthe Samandra, University of Melbourne</li>
</ul>
<h5>10:20 a.m. - <strong>Advancing Analysis: Eurofins&#39; Path to ISO 17025 Accreditation for Microplastics Testing</strong></h5>
<ul>
<li>Julia Jäger, Eurofins</li>
</ul>
<h5>10:40 a.m. - Coffee Break</h5>
<h5>11:00 a.m. - <strong>Validation and Application of LDIR Imaging to Analyze Microplastics in Marine Samples</strong></h5>
<ul>
<li>Lars Hildebrandt, Hereon</li>
</ul>
<h5>11:20 a.m. - <strong>Applying Multiple Analytical Techniques to Answer Open Questions About Offshore Wind</strong></h5>
<ul>
<li>Alexa Zonderman, Hereon</li>
</ul>
<h5>11:40 a.m. - <strong>Sample-Based Subsampling Strategies to Analyze Large Quantities of Particles in LDIR</strong></h5>
<ul>
<li>Manuel Andrade, University La Coruna</li>
</ul>
<h5>12:00 p.m. - <strong>Beyond the Surface: Spotlight on Plastic [Metal(loid)] Additive Leaching and Unseen Contaminants</strong></h5>
<ul>
<li>Ole Klein, Hereon</li>
</ul>
<h5>12:20 p.m. - <strong>Microplastics back in food- Advantages of Thermal Desorption for a wide range of Samples</strong></h5>
<h5>12:40 p.m. - <strong>Struggling with Solvent Effects During the Analysis of PFAS</strong></h5>
<ul>
<li>Dr. Axel Ritter, Agilent Technologies</li>
</ul>
<h5>1:00 p.m. - Lunch Break</h5>
<h5>2:00 p.m. - <strong>An Analytical Toolbox to Unravel PFAS Source Fingerprints</strong></h5>
<ul>
<li>Frank Menger, Hereon</li>
</ul>
<h5>2:20 p.m. - <strong>LDIR for Assessment of Microplastics in the VAMAS Ring Trial and Tissue in a Murine Infection Model</strong></h5>
<ul>
<li>Christoph Krafft, IPHT</li>
</ul>
<h5>2:40 p.m. - <strong>Label-Free Infrared Spectroscopic Micro- and Nanoplastics Detection in Complex Matrices</strong></h5>
<ul>
<li>Markus Brandstetter, Recendt</li>
</ul>
<h5>3:00 p.m. - <strong>Break in Dividing the Guests into Subgroups</strong></h5>
<h5>3:10 p.m. - <strong>Lab Visits: Organic Lab, Microplastics Lab, Sample Prep, LDIR, and Other Techniques</strong></h5>
<h5>4:50 p.m. - Wrap up</h5>
<h3><strong>Meeting Information</strong></h3>
<ul>
<li><p><strong>Date:</strong> 29 October 2024</p>
</li>
<li><p><strong>Time:</strong> 9:00 a.m. to 4:50 p.m. CEST</p>
</li>
<li><p><strong>Location:</strong> GITZ GmbH, Max-Planck-Straße 2, 21502 Geesthacht, Germany </p>
</li>
<li><p><strong>Meals included:</strong> Enjoy complimentary lunch and snacks during the event.</p>
</li>
</ul>
<p><strong>Limited spots available!</strong> Hurry, to secure yours today.</p>
<blockquote>
<p><a href="https://explore.agilent.com/microplameeting24#form"><strong>Secure your spot</strong></a></p>
</blockquote>


Agilent Microplastics Meeting 2024

We'll explore the latest trends and innovations in microplastics analysis.

<p><strong>We kindly invite you to join us for this live webinar: An Introduction to Nanoscale Infrared Spectroscopy and Imaging with Photothermal AFM-IR.</strong><br><br>Infrared (IR) spectroscopy is a commonly used characterization technique for organic materials and many inorganic compounds and crystals. While powerful, it is limited by the diffraction limit to resolution on the order of several microns, and the lateral resolution is strongly dependent on the wavelength.<br><br>Photothermal AFM-IR&nbsp;is a novel technique which offers IR spectroscopy and imaging on materials with a lateral resolution 1000x better than traditional FTIR and &lt;1 nm detection sensitivity with model-free interpretation. AFM-IR achieves this by using an AFM probe as the detector of photothermal expansion. This opens a variety of applications on both organic and inorganic materials where nanometer sensitivity and resolution are key.</p><div><br><strong>In this webinar we will:</strong></div><ul><li>Introduce the photothermal AFM-IR technique</li><li>Explore the surprisingly simple mechanism underlying the technique</li><li>Highlight a few of its many broad applications</li><li>Offer a demonstration of photothermal AFM-IR on the Dimension IconIR system</li></ul><blockquote><p><strong>Presenter:</strong> Peter De Wolf, Ph.D., (Director of Technology &amp; Application Development)</p><p>Peter De Wolf is director for AFM technology &amp; application development at Bruker Nano Surfaces, covering all applications related to Scanning Probe Microscopy (SPM). He obtained his PhD from IMEC, Belgium on the development of new SPM methods for 2D carrier profiling in semiconductors and has more than 25 years of experience on SPM. He is the author and co-author of over 30 publications related to electrical characterization using SPM. He also owns several SPM patents, and developed several new SPM modes for electrical characterization.</p><p><strong>Presenter:</strong> Cassandra Phillips, Ph.D. (Application Scientist, Bruker)</p><p>Cassandra did her Ph.D. at the University of Toronto exploring the photophysics of boron nitride nanotubes using scattering scanning nearfield optical microscopy (s-SNOM) and computational models. She has been working at Bruker Nano Surfaces and Metrology since September 2019 as an Applications Scientist focusing on nanoscale IR spectro-microscopy and other correlated imaging techniques realized with atomic force microscopy.</p><p><strong>Presenter: </strong>Dr. Qichi Hu, (Senior Applications Scientist )</p><p>Qichi is currently a Senior Staff Applications Scientist at Bruker Nano. He received a bachelor’s degree from Peking University and Ph.D. from University of British Columbia. He then did postdocs in U.S. university and national labs. Qichi has been working on nanoIR development and applications for over a decade, at Anasys and now at Bruker.</p></blockquote>

Bruker: Introduction to Nanoscale Infrared Spectroscopy and Imaging with Photothermal AFM-IR

Introduction to Nanoscale Infrared Spectroscopy and Imaging with Photothermal AFM-IR

An Introduction to Nanoscale Infrared Spectroscopy and Imaging with Photothermal AFM-IR.

Nanoscale IR Spectroscopy / Imaging with Photothermal AFM-IR

<p><strong>In this webinar, presenters will cover recent advances in in-situ nanomechanics testing, highlighting a case study on fracture toughness in nitride hard coatings. They will also introduce the PI 89 Auto PicoIndenter—which automates nanoindentation mapping for correlating microstructure with mechanical properties—and explore processing-structure-mechanical property relationships using this high-throughput in-situ system.</strong></p><p><strong>Join us for this webinar to see:</strong></p><ul><li>Research on the influence of columnar grain boundaries on the fracture toughness of nitride hard coatings, determined using in-situ SEM microcantilever fracture tests</li><li>An introduction to the PI 89 Auto PicoIndenter for streamlined correlation of microstructure and mechanical properties</li><li>Evaluation of processing-structure-property relationships in structural materials as determined using PI 89 Auto to correlate EBSD, EDS, and nanoindentation maps</li></ul><p><strong>Presenter:</strong> Subin Lee, Ph.D. (Group leader, Applied Materials Karlsruhe Institute of Technology)</p><p><strong>Presenter:</strong> Kevin Schmalbach, Ph.D. (Instrumentation Scientist, Nanoindentation Unit Bruker)</p>

Bruker: The latest advances in in-situ SEM nanoindentation testing

The latest advances in in-situ SEM nanoindentation testing

This webinar will cover recent advances in in-situ nanomechanics testing, highlighting a case study on fracture toughness in nitride hard coatings.

<p><strong>This live webinar will focus on applying Automated Atomic Force Microscopy (AFM) in inline metrology of hybrid bonding in the semiconductor industry. Bruker’s fully automated AFM solutions&nbsp;enable the highly accurate, non-destructive, nanoscale characterization of surfaces, while delivering high-throughput data for inline process control and actionable data for hybrid bonding yield enhancement.</strong></p><div><p>In this webinar, we will illustrate how Automated AFM can be applied in the most current hybrid bonding technology nodes and wafer processing steps, and its suitability for labs and fabs working on new bonding device design. The following topics will be discussed:</p><ul><li>Key Automated AFM features for process control in high volume manufacturing of bonded wafers</li><li>High resolution imaging and analysis for critical bond pad metrology</li><li>Large-area scanning of areas of up to 100s of mm2&nbsp;for large scan topography in wafer-to-wafer bonding</li><li>Automated bevel edge metrology for hybrid bonding</li><li>Patterned and bare/blanket wafer defect review</li></ul><p>Don’t miss the opportunity to speak to our experts in the Q&amp;A session!</p><blockquote><p><strong>Presenter: </strong>Sean Hand (Senior Staff Applications Scientist, Bruker)</p><p>Sean Hand is a Senior Staff Applications Scientist with Bruker's Automated AFM group focused on the application of AFM to semiconductor manufacturing. Sean has an M.S. in physics from the University of Vermont where he focused on Atomic Force Microscopy of lipid bilayers for transdermal drug delivery. Sean has over 25 years of experience in AFM for semiconductor manufacturing and has co-authored over 20 papers and owns several patents related to Automated AFM in semiconductor manufacturing.</p><p><strong>Presenter: </strong>Ingo Schmitz, Ph.D. (Technical Marketing Engineer, Bruker)</p><p>Ingo Schmitz received his Ph.D. in Chemistry from the University of Technology, Vienna Austria in 1996. His Ph.D. work focused on the development of SPM-based in-situ characterization of corrosion processes of glass and metal surfaces. Since 1997, Ingo Schmitz works at Bruker (formerly Digital Instruments &amp; Veeco Instruments) in both Santa Barbara, USA. At Bruker, he held positions as an R&amp;D engineer and application development scientist and contributed in the development of SPM methods including automated surface characterization of polysilicon. Currently, he is working in the technical marketing of automated AFM systems.</p></blockquote><p>&nbsp;</p></div>

Bruker: Automated AFM for Inline Hybrid Bonding Metrology in the Semiconductor Industry

Automated AFM for Inline Hybrid Bonding Metrology in the Semiconductor Industry

Learn how automated AFM can be applied to the most current hybrid bonding technology nodes and wafer processing steps

Automated AFM for Inline Hybrid Bonding Metrology

<div><p><strong>Join us and our special guest speaker Dr. Thomas Stroh, Assistant Professor at Montreal Neurological Institute, McGill University, Canada, for this webinar on Nanoscopic Imaging of Neuroendocrine Regulatory Processes Using Super-Resolution Microscopy. Dr. Thomas Stroh is a leading researcher in the field of G protein-coupled receptors. His work focuses on studying the mechanisms governing the availability of receptors at the cell surface of neurons and of neuroendocrine cells, factors that can ultimately determine their sensitivity to transmitters, neuropeptides, and active agents.</strong></p><p>Don’t miss the opportunity to hear Dr. Stroh give an overview of his research and innovative work on:</p><ul><li>Using the super-resolution microscopy techniques dSTORM (direct Stochastic Optical Reconstruction Microscopy) and biplane SMLM (single molecule localization microscopy) to image neuroendocrine regulatory processes in the brain at the near-molecular level</li><li>Combining super-resolution microscopy with plasma hormone level testing to study receptor trafficking and synaptic remodeling in neuroendocrine circuits in pituitary cells and brain tissue</li><li>Quantification of synaptic remodeling under the influence of hormonal secretion cycles in mouse brains</li></ul><blockquote><p><strong>Presenter:</strong> Thomas Stroh, Ph.D. (Associate Professor, Montreal Neurological Institute, McGill University)</p><p>Assoc. Prof. Thomas Stroh has over 20 years of experience working in the fields of microscopy and neurobiology. In 2005, he established the Neuro Microscopy Core Facility of the Montreal Neurological Institute (The Neuro) of McGill University, which has since grown into a modern microscopy platform housing advanced microscopy ranging from live cell microscopy to fluorescence super-resolution microscopy. It currently serves &gt;50 laboratories from The Neuro, various McGill departments, other Montreal universities and industry. Dr. Stroh has taught in numerous workshops and microscopy summer schools, including the Montreal Light Microscopy Course and the Canadian Light Microscopy Course at the University of Calgary. Together with Dr. Claire Brown of McGill University’s Advanced BioImaging Facility (ABIF), he organized the first Canadian Quantitative Super-Resolution Microscopy Course at The Neuro in 2019. Dr. Stroh is on the Executive Board of the Canada BioImaging Network. His research focuses on studying the regulation and trafficking of G Protein-Coupled Receptors. More recently, the Stroh lab is combining highly sensitive plasma hormone level testing with super-resolution microscopy to study the effects of endogenous rhythms on synaptic connectivity and plasticity in the hypothalamus.</p></blockquote><div><div><div><h3>Presenter's Abstract</h3><h4>Nanoscopic Imaging of Neuroendocrine Regulatory Processes in the Hypothalamo-Pituitary Axis Using dSTORM</h4></div></div></div><div><div><div><p>Single molecule localization microscopy (SMLM) using direct Stochastic Optical Reconstruction Microscopy (dSTORM) enables the imaging of regulatory processes in the brain at the near-molecular level, which cannot be resolved using traditional fluorescence microscopy techniques. However, imaging brain samples is challenging due to the optical density of the samples. Nevertheless, suitable tissue clearing, biplane imaging to capture 3D data, as well as computational and statistical methods to differentiate between signal and noise can overcome these difficulties.&nbsp;</p><p>Here, we used biplane SMLM and dSTORM in combination with highly sensitive determination of plasma hormone levels to investigate receptor trafficking and synaptic remodeling within the hypothalamo-pituitary endocrine circuit in endocrine pituitary cells and brain tissue, respectively. In corticotropic pituitary cells, we identified a novel syntaxin-6 positive compartment that is distinct from the trans-Golgi-network (TGN) by dSTORM. This compartment facilitates the regulated recycling of the somatostatin receptor subtype 2 (SSTR2) to the cell surface following Corticotropin-Releasing Hormone stimulation, using nearest-neighbor analysis to determine the size of this compartment. In the hypothalamus, we used dSTORM to image synaptic connectivity and its remodeling in sections through the arcuate nucleus of the mouse hypothalamus in parallel with the pulsatile rhythm of Growth Hormone (GH) secretion, employing biplane imaging and the DBSCAN clustering algorithm. We observed that during peak GH secretion, there were more excitatory synapses at the somata of GH-releasing hormone (GHRH) neurons.</p><p>Conversely, during periods of low GH levels, inhibitory synaptic input increased on GHRH cells. The results presented here highlight biplane imaging and dSTORM as a valuable quantitative approach to study receptor trafficking and synaptic structure in neuroendocrine circuits. Using appropriate sample preparation, SMLM imaging techniques, and careful statistical analysis we achieved a nanometer-scale precision in identifying a novel compartment in the pituitary cells as well as imaging and quantifying synaptic remodeling under the influence of hormonal secretion cycles in mouse brain.</p></div></div></div></div>

Bruker: Nanoscopic Imaging of Neuroendocrine Regulatory Processes Using Super-Resolution Microscopy

Nanoscopic Imaging of Neuroendocrine Regulatory Processes Using Super-Resolution Microscopy

Using super-resolution microscopy to reveal the inner workings of neuronal and neuroendocrine cells.

Nanoscopic Imaging of Neuroendocrine Regulatory Processes

<p><strong>Capturing Dynamic Insights with Live Cell Imaging</strong></p><p>During this webinar, guest speaker Dr. Helge Ewers, Professor for Membrane Biochemistry at the Freie Universität Berlin Institute for Chemistry and Biochemistry, will discuss his work investigating plasma membrane (PM) dynamics. Learn how single particle tracking and super-resolution microscopy unravel how actin rings influence the PM of cells.&nbsp;</p><p><strong>Presenter's Abstract</strong></p><p>The compartmentalization of the plasma membrane (PM) is a fundamental feature of cells. The diffusivity of membrane proteins is significantly lower in biological than in artificial membranes. This is likely due to actin filaments, but assays to prove a direct dependence remain elusive. We recently showed that periodic actin rings in the neuronal axon initial segment (AIS) confine membrane protein motion between them. Still, the local enrichment of ion channels offers an alternative explanation. Here we show, using computational modeling, that in contrast to actin rings, ion channels in the AIS cannot mediate confinement. Furthermore, we show, employing a combinatorial approach of single particle tracking and super-resolution microscopy, that actin rings are close to the PM and that they confine membrane proteins in several neuronal cell types. Finally, we show that actin disruption leads to loss of compartmentalization. Taken together, we here develop a system for the investigation of membrane compartmentalization and show that actin rings compartmentalize the PM.</p><blockquote><p><strong>Presenter:</strong> Helge Ewers, Ph.D. (Professor for Membrane Biochemistry, Freie Universität Berlin Institute for Chemistry and Biochemistry)</p></blockquote>

Bruker: Control of Membrane Protein Motion by Submembrane Actin Ring

Control of Membrane Protein Motion by Submembrane Actin Ring

Learn how single particle tracking and super-resolution microscopy unravel how actin rings influence the plasma membrane of cells.

<p><strong>In this webinar, Bruker nanoscale IR spectroscopy experts show how photothermal AFM-IR can identify the local chemistry of defects. With this detailed knowledge about chemistry, defect origins can be identified and eliminated.</strong></p><p>AFM-IR is a unique, automation-capable technique that reaches beyond the capabilities of bulk spectroscopy or nanoscale elemental analysis methods. It has redefined the boundaries of nanoscale characterization for the semiconductor manufacturing industry.</p><p><strong>Join us for this webinar to learn:</strong></p><ul><li>What AFM-IR is and how it works</li><li>Which parts of the semiconductor manufacturing process could be improved using nanoscale chemical analysis</li><li>What level of automation can be achieved with Bruker’s AFM-IR systems</li></ul><h5>Webinar Abstract</h5><div><p>Semiconductor devices are an essential part of our modern world. Current challenges exist in reducing contamination on wafers and masks, refining existing deposition, etch and growth techniques and improving EUV lithography and photoresist processes. The ability to accurately characterize and measure these materials is critical to improving wafer yields and pushing towards smaller transistors and developing new packaging solutions. Elemental analytical techniques such as EDS/EELS have stepped up to meet the need for higher sensitivity and resolution, but lack chemical specificity in identifying organic materials. AFM-IR is up to the task to identify the origin and narrow down to one of the many processing/inspection steps during which defects may be introduced.</p><p>AFM-IR (atomic force microscopy infrared spectroscopy) combines AFM’s high spatial resolution with IR spectroscopy’s chemical specificity, and has been used by many semiconductor companies to improve product outcomes. Bruker’s Dimension IconIR and Dimension IconIR300 systems work as multifunctional instruments for studying the nanoscale chemistry, along with electrical and mechanical properties of semiconductor devices on wafers up to 300 mm. Automation capabilities and the capability to import coordinates from KLARF allows for considerably higher throughput and the best cost of ownership for nanoscale IR imaging and spectroscopy.</p><p>This webinar highlights AFM-IR's applications in defect identification, process control, and photoresist characterization, and its role in advancing 2D material research for the semiconductor industry. Join us to discover how AFM-IR is redefining the boundaries of nanoscale characterization, and the unique role it can play in semiconductor manufacturing, and how AFM-IR is being used to explore the future of semiconductor devices.</p></div><blockquote><p><strong>Presenter: </strong>Dr. Qichi Hu (Senior Applications Scientist)</p><p>Qichi is currently a Senior Staff Applications Scientist at Bruker Nano. He received a bachelor’s degree from Peking University and Ph.D. from University of British Columbia. He then did postdocs in U.S. university and national labs. Qichi has been working on nanoIR development and applications for over a decade, at Anasys and now at Bruker.</p><p><strong>Presenter:</strong> Chunzeng Li, Ph.D. (Applications Engineer, Bruker)</p><p>Dr. Chunzeng Li is an Applications Engineer with Bruker, Nano Surfaces and Metrology Division, with over 30 years AFM experience. He received his PhD in Physical Chemistry from Xiamen University, China, and is the author of 31 international publications and 2 patents.</p><p><strong>Presenter: </strong>Jin Hee Kim, Ph.D. (Applications Scientist, Bruker)</p><p>Dr. Jinhee Kim is a NanoIR Applications Scientist at Bruker Nano. She received her Ph.D. in materials chemistry from the University of Michigan and completed her postdoctoral appointment at Monash University in Australia. She specialized in nanoIRs since her time in academia and is continuing to apply NanoIR to various fields.</p></blockquote>

Bruker: Nanoscale Chemical Characterization of Semiconductor Materials and Devices using Photothermal AFM-IR

Nanoscale Chemical Characterization of Semiconductor Materials and Devices using Photothermal AFM-IR

In this webinar, Bruker nanoscale IR spectroscopy experts show how photothermal AFM-IR can identify the local chemistry of defects.

ICPMS webinars focusing on Microscopy | LabRulez ICPMS