<div><p><strong>In 2025, two outstanding young female researchers, Drs. Filipa Oliveira and Stella Gonsales, both originally from outside the Czech Republic, received prestigious JUNIOR STAR grants from the Czech Science Foundation (GAČR). JUNIOR STAR enables early career researchers to establish their own independent research groups and significantly advance their scientific careers. Their projects reflect cutting-edge research in materials science and sustainable chemistry, highlighting the international reach and welcoming research environment at UCT Prague.</strong></p><p><strong>Dr. Stella Gonsales is also a&nbsp;laureate of the Dagmar Procházková Fund, which supports promising scientific projects in sustainable and advanced organic chemistry. Another recipient of this grant is Dr.&nbsp;Anna Valunchene, whose research complements UCT Prague’s&nbsp;sustainability goals. This dual funding underlines Stella’s&nbsp;remarkable achievements and the wide scope of innovative research at UCT Prague.</strong></p><p><strong>Both Filipa and Stella are actively looking for motivated students and researchers to join their teams, offering a&nbsp;unique opportunity to work at the forefront of science in a&nbsp;vibrant and supportive environment.</strong></p></div><div><h3><strong>Interview with Filipa Oliveira: Pioneering tubular MXenes for supercapacitor energy storage</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Filipa Oliveira" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg 235w," sizes="100vw" width="1500" height="999"></p><h5><strong>Congratulations on receiving the JUNIOR STAR grant. What does this achievement mean for you and how will it influence your next steps?</strong></h5><p>Thank you. It’s&nbsp;a huge achievement to receive this prestigious grant. It gives me the opportunity to establish my own research group here at UCT Prague. The JUNIOR STAR program supports talented young scientists in building their teams and accessing resources. This means I&nbsp;now have the support to focus fully on my research about tubular MXenes and their application in supercapacitors.</p><h5><strong>Why are tubular MXenes so innovative for energy storage?</strong></h5><p>MXenes are a family of 2D materials with a layered, accordion-like structure and known for their excellent electrical conductivity. Our innovation is to produce them with a tubular morphology, combining the high conductivity and redox activity in superior ion-transport tubular architectures. Their open, hollow structure increases surface area, ion diffusion and charge transport. This could translate to faster, more efficient supercapacitors, critical for sustainable energy technologies.</p><h5><strong>How did your scientific interest lead you to this field?</strong></h5><p>Global challenges around green energy made me focus on materials that can improve energy storage. MXenes stood out because changes in the composition and structure can significantly change their properties. I&nbsp;was curious about how tuning different metallic elements in a&nbsp;tubular morphology can impact how MXenes perform and how to optimize them for practical applications.</p><h5><strong>What challenges have you encountered?</strong></h5><p>One major challenge is their stability: MXenes oxidize quickly which deteriorates performance. Another challenge is understanding how synthesis conditions of both MXenes and their precursors (MAX phases) must be adapted to create and preserve tubular morphologies since their synthesis mechanisms differ from conventional MXenes. Additionally, effectively communicating the project in the funding proposal to show to reviewers why I&nbsp;should obtain this funding was also a&nbsp;challenge, but thanks to mentorship and collaboration, I&nbsp;overcame these hurdles.</p><h5><strong>What motivates you most about working at UCT Prague?</strong></h5><p>The support from colleagues and the excellent technical infrastructure make it a&nbsp;great place to work. Also, being a&nbsp;former member of Professor Sofer’s&nbsp;leading group in 2D&nbsp;materials is inspiring and led me to this achievement.</p><h5><strong>How do&nbsp;you see your professional development going in the future?</strong></h5><p>The grant will help me build my own team, acquire equipment, and develop collaborations. My long-term vision is to contribute significantly to sustainable energy solutions and scientific progress both locally and internationally.</p><h5><strong>You are looking for new team members. What kind of students or scientists do&nbsp;you need?</strong></h5><p>We are currently welcoming enthusiastic master's&nbsp;students, PhD candidates, and postdocs who are passionate about materials science and eager to explore new frontiers in MXenes and energy storage. If you love curiosity-driven research and want to be part of a&nbsp;growing international team, we encourage you to join us.</p><h3><strong>Interview with Stella Gonsales: Catalysis as a&nbsp;key to polymer recycling</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Stella Gonsales" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg 233w," sizes="100vw" width="1499" height="1000"></p><h5><strong>Congratulations on your JUNIOR STAR grant and also being awarded the Dagmar Procházková Fund. What does receiving both mean to you and how will they shape your work?</strong></h5><p>Thank you. Both grants are hugely important. The JUNIOR STAR grant allows me to expand my group and resources for research into catalytic recycling of polymers, while the Dagmar Procházková Fund supports complementary projects such as synthesizing new biodegradable polymers. Together, they help tackle the plastic waste problem from multiple scientific angles.</p><h5><strong>Could you explain your research in layperson’s&nbsp;terms?</strong></h5><p>Most plastics have strong carbon-carbon bonds. We insert more cleavable double bonds into polymers that can be broken down by catalytic reactions called olefin metathesis, making recycling on a&nbsp;molecular level more feasible.</p><h5><strong>What makes the JUNIOR STAR grant particularly important for your research and career development?</strong></h5><p>The JUNIOR STAR grant is a&nbsp;prestigious and highly competitive funding opportunity aimed at excellent early-career scientists within eight years of completing a&nbsp;Ph.D. It provides up to 25&nbsp;million CZK over five years, allowing recipients to establish independent research groups and pursue innovative scientific topics. This grant is crucial for building a&nbsp;solid foundation, acquiring necessary equipment, and recruiting talented team members, enabling the pursuit of ambitious projects that can significantly contribute to both the Czech scientific community and global challenges.</p><h5><strong>What collaborations support your research?</strong></h5><p>The collaboration between UCT Prague and the Institute of Organic Chemistry and Biochemistry (IOCB) of the Czech Academy of Sciences primarily provides financial and technical support. The cooperation helps with funding, access to advanced equipment, and operational resources needed for running research projects.</p><h5><strong>What advice would you give young scientists aiming to succeed?</strong></h5><p>I&nbsp;encourage thinking outside the box and combining diverse knowledge you acquire during your career to address complex problems that are exciting and relevant to you.</p><h5><strong>Your laboratory bears the name of Otto Wichterle and was ceremonially opened in the presence of Professor Kamil Wichterle. How does Wichterle’s&nbsp;legacy inspire you personally?</strong></h5><p>Wichterle’<s>’</s>s&nbsp;innovative spirit motivates me to push creative boundaries and pursue novel solutions in sustainable chemistry.</p><h5><strong>What are your visions for the future?</strong></h5><p>I&nbsp;aim to build a&nbsp;research group that advances sustainable polymer recycling, contributing to environmental responsibility and the circular economy.</p><h5><strong>You are recruiting people for your team. What skills do&nbsp;they need to have?</strong></h5><p>We are looking for talented Ph.D. students and postdocs experienced in air-sensitive catalysis techniques. If you want to contribute to transformative research in sustainable chemistry and join a&nbsp;dynamic team, this is a&nbsp;great opportunity.</p></div>

JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science

<p><strong>The clean laboratories of the CEITEC Nano research infrastructure at CEITEC Brno University of Technology house a four-meter-tall TITAN transmission electron microscope for researching materials at the atomic level. This scientific device underwent two significant upgrades this year, entailing the addition of two state-of-the-art technologies: an electromagnetic lens aberration corrector for scanning transmission electron microscopy and an electron energy loss spectrometer equipped with cameras featuring today's most sensitive sensors. Thanks to these upgrades, TITAN has become a state-of-the-art device that can only be found in a few of the world's most advanced laboratories in this configuration.</strong></p><p><strong>The TITAN Themis microscope </strong>was manufactured in 2015 by FEI (now Thermo Fisher Scientific) and has been in use at CEITEC BUT since 2016. At the time of its acquisition, it was one of<strong> the most powerful commercially available electron microscopes</strong>; however, it lacked a corrector for optical defects in the lenses that form the narrow electron beam scanning the sample. This meant limited resolution and a lower ability to analyze the chemical composition and physical properties of materials at the atomic level.</p><p><i>"The latest generation S-CORR aberration corrector allows the electron beam to be focused to a size up to twice smaller than an atom, while also providing a higher electron current. This has led to a significant increase in the resolution and analytical capabilities of the microscope,"</i> explains <strong>Jan Michalička</strong>, guarantor of the TITAN microscope. "<i>Thanks to the new corrector, our microscope achieves the world's highest standard in resolution, specifically less than 50 picometers at an acceleration voltage of 300 keV." </i>For better understanding, he adds a comparison: <i>"Imagine the ratio of the size of a golf ball to that of planet Earth. That is exactly the ratio of an atom measuring 100 pm to a golf ball."</i> Thanks to this resolution, scientists are not only able to image individual atoms, but also to determine their chemical nature through further analysis. This is of fundamental importance, for example, for the development of new catalysts for a more environmentally friendly chemical industry.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Differences in resolution before and after the installation of the aberration corrector." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg 245w," sizes="100vw" width="1600" height="904"></p><p>The new corrector also allows the microscope to achieve high resolution even with low-energy electrons. This is crucial for studying highly sensitive materials such as graphene and other two-dimensional structures, which would be damaged by a stronger electron beam. <i>"Electrons interact strongly with the sample and can damage it at the atomic level, for example, by changing its structure and chemical composition. With the new corrector, we can achieve atomic resolution even at the lowest acceleration voltage of 60 keV, allowing us to study even very sensitive materials in a virtually unchanged state," </i>says Jan Michalička.</p><h4><strong>The additional installation of a corrector at the CEITEC laboratory is unique worldwide</strong></h4><p><strong>Correctors of optical defects in electromagnetic lenses are an exceptional technology</strong> that has completely changed the world of electron microscopy. For a long time, they were considered technically impossible, and the absence of correctors essentially prevented research into the structure and properties of materials at the atomic level. It was not until the 1990s that a group of four scientists – Harald Rose, Max Haider, Knut Urban, and Ondřej Křivánek – succeeded in implementing this technology, for which they received the prestigious Kavli Prize in 2020. <i>“Correctors basically consist of a pair of complex lenses, each containing a series of magnetic multipoles. In the case of the correctors invented by the first three scientists mentioned above, defects are removed by two hexapole lenses. Our new corrector is based on the same concept,”</i> adds Jan Michalička.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Delivered aberration corrector prior to installation, with the TITAN microscope in the background." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><p>The additional installation of a corrector is <strong>unique </strong>in itself nowadays. Similarly complex technologies are usually installed during the manufacture of microscopes in the factory, in this case in the Netherlands. However, in Brno, the microscope was successfully upgraded with a corrector under the limited conditions of the laboratory. The installation of the corrector took about four months and required the dismantling of a large part of the microscope. <i>"With this installation, we basically went back to the time when the four scientists mentioned above placed their prototype corrector on an older microscope, thus improving its performance,"</i> says Jan Michalička with a smile, adding that the most difficult part was the precise adjustment of the corrector and the entire optics of the microscope in order to achieve top parameters. <i>"Even though most things are now controlled by computers, there are only a few people in the world who can install and tune a corrector to the highest level of accuracy. We are therefore extremely pleased to have had such experts at our disposal here."</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the aberration corrector – removal of the condenser." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><h4><strong>New spectrometer will enable scientists to see more</strong></h4><p>The second and equally important upgrade to the TITAN microscope is the installation of <strong>the latest generation GIF Continuum HR electron energy loss spectrometer by Gatan</strong>. This is an approximately two-meter-long device installed horizontally under the microscope, whose main component is a magnetic prism that separates the electron beam according to their energy losses. The spectra of these losses provide enormous information about the sample, principally at the atomic level, as they are characteristic of the given state of the atoms, such as the valence arrangement of their electron shell. In combination with the new corrector, the spectra can be used to analyze the chemical bonds, arrangement in the crystal lattice, or the entire range of physical properties of atomic nanostructures down to the level of individual atoms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the spectrometer beneath the microscope, magnetic prism." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><p>An essential part of the spectrometer are the <strong>state-of-the-art K3 and Stela cameras</strong>, which can directly detect and count individual electrons. At the same time, these cameras are extremely fast and their sensors capture data without noise. <i>"These features of our cameras are key to measuring sensitive or dynamically changing samples and allow us to obtain information that was previously invisible to electron microscopes,"</i> says Jan Michalička.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installed high-performance spectrometer cameras." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><p>Last but not least, the spectrometer will open up completely new possibilities for measuring the magnetic properties of atomic structures. The new spectrometer will also be used for such experiments within the project TERAFIT, which financed this upgrade, and which focuses on research into materials for ultra-high capacity, speed, and energy efficiency in information technology. <i>"The device is fully prepared for advanced experiments and the use of modern software tools, including artificial intelligence,"</i> adds <strong>Vojtěch Uhlíř</strong>, head of the <i>Nanomagnetism and Spintronics</i> research group at CEITEC BUT and one of the TERAFIT project investigators, highlighting the advantages of the spectrometer.</p><p>Together with the corrector, the spectrometer represents a breakthrough in the analysis of material properties, and according to Jan Michalička, CEITEC now has one of the most powerful microscopes available. <i>“In modern materials science, for example, in the development of magnetic memories, quantum materials, or 2D structures, it is necessary to see not only atoms, but also their behaviour in real time, including magnetic states. And it is this spectrometer that pushes the boundaries of what we can see and analyze.</i>” Michal Urbánek, head of the CEITEC Nano laboratories and coordinator of the large research infrastructure <a target="_blank" rel="noopener noreferrer" href="https://www.czechnanolab.cz/"><strong>CzechNanoLab</strong></a>, agrees with him, adding that the device can now contribute to scientific knowledge on a global scale. <i>“Thanks to its integration into the large CzechNanoLab research infrastructure, this state-of-the-art microscope is accessible not only to scientists from CEITEC, but also to other institutions in the Czech Republic and abroad.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Final configuration of the TITAN microscope after installation of the aberration corrector and spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><blockquote><p>Do you want to know more? Read the<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/upgrade-of-titan-transmission-electron-microscope-ranks-ceitec-among-elite-laboratories-expert-version/t11696?secret=40ab0267-ec7e-11f0-8f5d-2ed9ab825d4b"><strong>expert version</strong></a><strong> </strong>of this article.</p></blockquote>

Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories

<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Anton Paar

Agilent Technologies

AZoM

Milestone

Bruker

Thermo Fisher Scientific

<p><strong>The strength of the Cary 7000 is its ability to make absolute specular reflectance measurements at angles ranging from 5.2 to 85 degrees. It also can make transmission measurements during the same collection sequence thereby ensuring the same location on the sample is being interrogated. This provides the high-quality data necessary for R&amp;Q work. The single sample holders available also allow the user the ability to QC their production runs. While time consuming, all parts of a production can be analyzed to limit return of product. The addition of an Autosampler facilitates the increase in productivity of a QC run while maintaining the workflow.</strong></p><p>During this presentation, we will look at the aspects of adding and using an autosampler to Maximize QC Workflow.</p><blockquote><p><strong>Presenter: </strong>Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)</p><p>Mark, an Application Scientist for Agilent Technologies, has over 30 years of experience in molecular spectroscopy. He has held roles in Agilent's UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark's responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.</p></blockquote>

Agilent Technologies: Moving from R&D and Limited QC on a UV-Vis-NIR instrument to Large Scale QC Using an Autosampler

Moving from R&D and Limited QC on a UV-Vis-NIR instrument to Large Scale QC Using an Autosampler

During this presentation, we will look at the aspects of adding and using an autosampler to Maximize QC Workflow.

From UV-Vis-NIR to Large Scale QC Using an Autosampler

<p><strong>This webinar will discuss accuracy and precision and how to ensure both.</strong></p><p>We will show live examples of precision examples and how to improve it. For accuracy we will show techniques like internal standards, standard addition, dilutions, duplicates, spikes and spike recoveries, second source standard type and there uses.</p><blockquote><p><strong>Presenter:</strong> Paul Krampitz (Application Scientist, Agilent Technologies, Inc.)</p><p>Paul is a graduate of Bowling Green State University with a Chemistry Bachelor of Science degree. Twenty – five years of Spectroscopy experience mainly Inductively Coupled Plasma (ICP) Spectroscopy as well as ICP-MS and AAS. His experience also includes X-Ray Fluorescence (ED, WD), Laser Ablation, Inorganic Speciation and Microwave Plasma Spectroscopy. Market Segment expertise includes environmental, petrochemical, metallurgical applications.</p><p><strong>Presenter:</strong> Luke Gormley (Pre-Sales Application Engineer, Agilent Technologies, Inc.)</p><p>Luke earned his Bachelor of Science in Chemistry from Clemson University in South Carolina. He began his career at an environmental contract testing laboratory, where he used EPA methods for routine ICP-OES analysis. Later, he expanded his role by joining the laboratory’s instrument service and repair team, troubleshooting a wide range of analytical instruments and testing equipment. In 2025 Luke joined Agilent Technologies, where he now works as an Application Scientist, supporting the ICP-OES, MP-AES, and AA product lines.</p></blockquote>

Agilent Technologies: Learn how to Confirm your Data on ICP-OES. Are you getting the Right Result? Are You Sure?

Learn how to Confirm your Data on ICP-OES. Are you getting the Right Result? Are You Sure?

This webinar will discuss accuracy and precision and how to ensure both.

Learn how to Confirm your Data on ICP-OES.

<p><strong>As the needs of the semiconductor industry call for being able to measure metals at lower and lower concentrations, it is necessary to employ the best tune conditions for each of the metals of interest.</strong></p><p>This means that multiple tune modes such as cool plasma, hot plasma, oxygen, ammonia, etc. are required when analyzing a full suite of elements thus necessitating the changing of RF power, positioning of the torch and gas flows within a single analysis. &nbsp;Not only is it important to demonstrate the ability to achieve low detection limits within these methods but also to demonstrate long term stability as the instrument cycles back and forth through the various tune conditions from sample to sample.</p><p>We will show examples of this with TMAH and UPW matrices.</p><blockquote><p><strong>Presenter:</strong> Bert Woods (Application Scientist, Agilent Technologies, Inc.)</p><p>Joined the Agilent ICP-MS team in 2004, with previous employment in the semiconductor industry with Dominion Semiconductor (IBM/Toshiba) and Micron. Bert is a 1997 Chemistry graduate of Radford University in Virginia and an avid Washington DC Sports fan.</p></blockquote>

Agilent: Agilent Webinar Series - Semiconductor and Specialty Chemical Industry

Novel Advancements in Trace Level Determination and Longterm Stability of Metals in TMAH and UPW matrices

Trace Determination and Stability of Metals in TMAH and UPW

<div><p><strong>The Compact-Q DEER Spectrometer is designed to support researchers in academia and industry to efficiently characterize quantum materials, develop devices for quantum sensing, advance and validate algorithms to control qubits, and other applications in spin-based quantum technologies. The Compact-Q DEER system features a modern microwave architecture, high-resolution AWG, and digital signal processing schemes enabling high flexibility in addressing modern challenges of quantum technologies.</strong></p><p>The focus of this webinar will be to highlight the capabilities of the Compact-Q DEER spectrometer towards the characterization and coherent control of qubits for quantum technologies. Modern experiments in Quantum Information Science (QIS) demand a state-of-the-art microwave architecture, fast, high-memory, high-resolution AWGs, large transmission and detection bandwidths, and modern digital signal processing features to create the most sophisticated pulse shapes, quantum gates and read-out sequences. Having all of those features incorporated, as well as operating at an intermediate frequency of 500 MHz, and utilizing a cryogen-free 1.5 T magnet with integrated Variable Temperature Insert (iVTI) enabling temperatures from 2 K to RT, the Compact-Q system meets modern demands and provides a versatile and high-fidelity platform for probing qubit properties and execution of quantum algorithms driving innovations in quantum technologies.</p><h4><strong>What to Expect</strong></h4><p>During this webinar, we will demonstrate how the Compact-Q DEER is ideally suited for applications in QIS. We will show how broad-band echo sequences can be used to manipulate spin states and how electron spin coherences can be created and detected. We will explain the different spectrometer features, the data acquisition platform along with different experiments, highlighting how the Compact-Q can elevate your research in quantum information science (QIS).</p><h4><strong>Key Learning Points</strong></h4><p>Versatility, high-performance, and user-friendly operation of the Compact-Q DEER spectrometer will be highlighted along with:</p><ul><li>Easy to use resonator bandwidth control</li><li>AWG generated pulses, digital down conversion, and FIR filtering</li><li>Pulse shaping and quantum gate optimization</li><li>Broadband and selective inversion pulse schemes</li><li>Studying spin dephasing mechanisms and materials characterization</li></ul><h4><strong>Who should attend?</strong></h4><ul><li>Undergraduate and graduate students in chemistry, physics, quantum information science, material science, and related fields&nbsp;</li><li>Early-career researchers, postdoctoral fellows, and instrument facility managers familiar or new to EPR spectroscopy&nbsp;</li><li>Professors and instructors teaching EPR spectroscopy or related courses&nbsp;</li><li>Spectroscopy facility managers</li><li>Industry professionals, scientists, and engineers in materials science and quantum technologies industries</li></ul><blockquote><p><strong>Presenter:</strong> Alvaro Montoya (Ph.D. in chemistry from the University of Florida, EPR Application Scientist, Bruker BioSpin)</p><p>Alvaro Montoya began his EPR career as a Ph.D. student at the University of Florida after obtaining his B.S. in Chemistry from Susquehanna University. Early in his graduate career, he made use of CW and High Field EPR in studying the catalytic mechanisms of metalloenzymes. He then grew a profound appreciation for FT EPR and expanded his EPR toolbox for his research endeavors. After completing his Ph.D., Alvaro joined Bruker in January of 2023 working as an Applications Scientist.</p><p><strong>Presenter:</strong> Dmitry Akhmetzyanov (Ph.D. in chemistry from Goethe University Frankfurt am Main, Research Scientist, Bruker BioSpin)</p><p>Dr. Akhmetzyanov began exploring EPR spectroscopy during his undergraduate studies at Kazan University, the birthplace of EPR. Throughout his Bachelor’s and Master’s programs, Dmitry’s work centered on applying EPR techniques to materials science. For his Ph.D., Dmitry joined Thomas Prisner’s group at Goethe University Frankfurt am Main, where he focused on development and applications of pulsed EPR dipolar spectroscopy to study structures and dynamics of macromolecules. After his research positions in Germany and Canada, Dmitry joined Bruker in 2024. In the current role as a Research Scientist, he focuses on advancing EPR spectroscopy, developing novel applications, and supporting customers.&nbsp;</p></blockquote></div>

Bruker: Meet Compact-Q DEER Spectrometer, Your Gateway to Quantum Innovation

Meet Compact-Q DEER Spectrometer, Your Gateway to Quantum Innovation

Webinar on Compact-Q DEER spectrometer for quantum information science, showcasing qubit control, pulse shaping, broadband EPR, and advanced DSP capabilities.

Compact-Q DEER Spectrometer, Gateway to Quantum Innovation

<div><div id="inner_content_body_text" data-content-id="394785"><p><strong>Join us for an exclusive live webinar introducing the new Litesizer DIF series of laser diffraction particle size analyzers. Designed for both liquid and dry dispersions, the Litesizer DIF delivers precise and reproducible particle size distributions across the nanometer to millimeter range.</strong></p><p>Learn how its best-in-class optical setup, Quick-Click dispersion units, and Kalliope software enable seamless, high-confidence measurements – whether for R&amp;D or quality control. Our experts will demonstrate how the system simplifies workflows, enhances repeatability, and minimizes manual effort to make advanced particle sizing accessible to every laboratory.&nbsp;</p><p><strong>Join the free webinar with certification of attendance now.</strong></p><h5><strong>Attend This Webinar to Learn:</strong></h5><ul><li>How Litesizer DIF laser diffraction technology enables full-range particle size analysis</li><li>How integrated dynamic image analysis module supports method development and troubleshooting, and helps interpret and validate results</li><li>Practical strategies for the use of dispersion methods &nbsp;(wet and dry measurements)</li><li>How Litesizer DIF’s Kalliope software supports workflow efficiency</li><li>Techniques to improve QC efficiency and reduce measurement variability</li><li>Real-world application examples from industrial and research environments</li></ul><h5><strong>Ideal For:</strong></h5><ul><li>Scientists, lab managers, and quality professionals in the fields of building materials, minerals, paints and coatings, batteries, and environmental analysis who are seeking to modernize their particle-sizing workflow with robust, flexible technology.</li></ul><blockquote><p><strong>Presenter:</strong> Dr. Aleksandra Mitrovic (Product Manager, Anton Paar GmbH, Graz / Austria)</p><p>Dr. Aleksandra Mitrovic holds a degree in biochemistry from the University of Sciences in Novi Sad, Serbia. She earned her PhD in molecular biotechnology from Graz University of Technology before going on to work in applied biocatalysis across various industrial projects. In 2017, Aleksandra joined Anton Paar as a global product manager for laser diffraction, where she has accumulated eight years of experience and continues to deepen her expertise in this specialized field.</p></blockquote></div></div>

Anton Paar: From First to Best: Introducing the Litesizer DIF Series

From First to Best: Introducing the Litesizer DIF Series

Join us for a webinar introducing the new Litesizer DIF series of laser diffraction particle size analyzers. 

<div><div id="inner_content_body_text" data-content-id="394785"><p><strong>Rotational and transient viscosity measurements on a rotational rheometer provide a powerful suite of characterization tools for the flow behavior of polymer melts. From research to quality control, viscosity is a key parameter when developing polymers and their processing techniques.</strong></p><p>This webinar focuses on the application of rotational and transient viscosity measurements to polymer melts, including the instrumentation and accessories, measurement techniques, and data interpretation or analysis.</p><blockquote><p><strong>Presenter:</strong> PhD Kiana Shirzad</p><p>Kiana Shirzad is an Application Scientist for Rheology and Extrusion at Anton Paar. She earned her PhD in Materials Science and Engineering from the University of California, Merced, where she focused on polymers and advanced material characterization methods. Her expertise lies in applying rheological techniques to understand complex material behavior and supporting customers in their research and industrial applications.</p></blockquote></div></div>

Anton Paar: Polymer Rheology: Rotational and Transient Measurements On A Polymer Melt

Polymer Rheology: Rotational and Transient Measurements On A Polymer Melt

Rotational and transient viscosity of polymer melts: rheometer techniques, instrumentation, accessories, and data interpretation for R&D and quality control.

Rotational and Transient Measurements On Polymer Melt

<p><strong>Environmental laboratories face increasing pressure to process complex matrices for both inorganic and organic contaminants with higher efficiency, lower cost, and full regulatory compliance. This four-part webinar series provides a comprehensive overview of modern sample preparation solutions for heavy metals, mercury, and organic pollutants, including PFAS.&nbsp;</strong></p><p>Through practical guidance and real-world examples, we will explore how advanced digestion, extraction, and direct analysis tools streamline workflows, minimize errors, and improve data quality. Participants will gain actionable strategies to enhance productivity and achieve more reliable environmental results across the entire sample-to-analysis journey.</p><h4><strong>Episode 2: Practical guidance to improve the journey from sample to heavy metals analysis for environmental labs</strong></h4><p>The quality and reliability of elemental analysis in environmental labs depend heavily on the steps performed before the sample reaches the analytical instrument. Choices related to acid purity, reagent dosing, filtration, dilution, and vessel cleaning all influence detection limits, blank levels, safety, and overall efficiency. This webinar provides practical, experience-driven recommendations to optimize each stage of the sampleto- analysis workflow. Attendees will learn how to minimize contamination, reduce manual operations, and adopt more cost-effective and productive approaches, ultimately improving data quality while lowering the total cost per sample.</p><blockquote><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://share.hsforms.com/1b7diMdH_QtaH4bNUuW_T9Q5dmem"><strong>Register HERE</strong></a></p></blockquote><blockquote><p><strong>🎤Speaker:</strong> Gianpaolo Rota (Application Specialist, Milestone Srl)</p></blockquote>

Milestone: Environmental Sample Prep Series 2026

Practical guidance to improve the journey from sample to heavy metals analysis for environmental labs

Improve heavy metals analysis in environmental labs with practical tips on reagents, filtration, cleaning, and workflow optimization.

Improve journey from sample to heavy metals analysis

<p><strong>Environmental laboratories face increasing pressure to process complex matrices for both inorganic and organic contaminants with higher efficiency, lower cost, and full regulatory compliance. This four-part webinar series provides a comprehensive overview of modern sample preparation solutions for heavy metals, mercury, and organic pollutants, including PFAS.&nbsp;</strong></p><p>Through practical guidance and real-world examples, we will explore how advanced digestion, extraction, and direct analysis tools streamline workflows, minimize errors, and improve data quality. Participants will gain actionable strategies to enhance productivity and achieve more reliable environmental results across the entire sample-to-analysis journey.</p><h4><strong>Episode 3: Reliable mercury analysis in environmental samples: tools, techniques, and best practices</strong></h4><p>Mercury analysis remains one of the most challenging tasks for environmental laboratories due to its volatility, contamination risks, and the complexity of traditional wet-chemistry workflows. This webinar presents a streamlined and highly reliable approach using Milestone’s DMA-80 direct mercury analyzer. Attendees will learn how to eliminate digestion steps, reduce handling of reagents, and improve data quality across solid and liquid environmental matrices. Practical guidance will be provided on controlling blank levels, avoiding common pitfalls, and meeting regulatory requirements while reducing cost per sample and simplifying day-to-day operations.</p><blockquote><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://share.hsforms.com/1b7diMdH_QtaH4bNUuW_T9Q5dmem"><strong>Register HERE</strong></a></p></blockquote><blockquote><p><strong>🎤Speaker:</strong> Valentina Carrara (Application Specialist, Milestone Srl)</p></blockquote>

Reliable mercury analysis in environmental samples: tools, techniques, and best practices

Episode 3 focuses on reliable mercury analysis in environmental samples, showing how direct analysis simplifies workflows, reduces contamination risks

Mercury analysis in environmental samples

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>This webinar, titled “Process Raman as a Reliable, Versatile Tool for Downstream Process Monitoring and mAb Production Control,” explores how process Raman spectroscopy can improve the efficiency of in-line monitoring of protein concentration.</strong></p><p>The presentation highlights how process Raman spectroscopy offers several advantages over traditional in-line UV-Vis analysis for process monitoring and metabolite production control. These advantages include both time and cost efficiencies, making Raman spectroscopy a powerful tool for enhancing downstream processes and production control.</p><h4><strong>You will learn:</strong></h4><ul><li>How process Raman spectroscopy is used for protein analysis</li><li>How to develop a Raman-based protein quantification model</li><li>The Raman model advantages over established UV-Vis monitoring practices</li></ul><blockquote><p><strong>Presenter: </strong>Nimesh Khadka, PhD (Sr. Product Application Specialist, Thermo Fisher Scientific)</p><p>Nimesh Khadka is a senior application scientist with at Thermo Fisher Scientific with specializing in analytical biochemistry, spectroscopy, and chemometrics. With more than 5 years of experience in process development and PAT, he currently champions Raman spectroscopy as a PAT for development of biotherapeutics therapeutics.</p><p>Nimesh completed his PhD at Utah State University focusing on enzymology, spectroscopy, and alternative bioenergy. In his postdoctoral training at Case Western Reserve University, Nimesh focused on the study of structural biology in biomolecules associated with eye pathologies. Through this extensive background, Nimesh has a deep understanding of the intricacies of biomolecular structures and their broad applications.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Process Raman: A Reliable and Versatile Tool For Downstream Process Monitoring and Control For mAB Production

Process Raman: A Reliable and Versatile Tool For Downstream Process Monitoring and Control For mAB Production

How process Raman spectroscopy offers several advantages over traditional in-line UV-Vis analysis for process monitoring and metabolite production control. 

Process Raman For Downstream Process For mAB Production

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>In regulated pharmaceutical manufacturing, rapid and reliable raw material identification is essential for maintaining product quality and optimizing GMP workflows. In this webinar hosted by Thermo Fisher Scientific, discover how the Thermo Scientific™ IonicX™ Portable XRF Analyzer is transforming ionic salt verification directly on the manufacturing line.</strong></p><p>Delivering lab-quality elemental analysis in under 2 minutes, IonicX provides real-time PASS/FAIL results for key salts, such as NaCl, KCl, MgCl₂, CaCl₂, and NaOH, helping reduce quarantine delays, streamline material transport, and improve at-line decision-making.</p><p>When paired with the TruScan™ Handheld Raman analyzer, the complementary capabilities of IonicX extend raw material verification coverage, enabling identification of ionic salts for comprehensive raw material ID and GMP compliance.</p><h4><strong>In this session you will see:</strong></h4><ul><li>A live demo of IonicX in action</li><li>How IonicX supports 21 CFR Part 11 and cGMP compliance</li><li>Integration of IQ/OQ/PQ documentation, annual certification, and safety interlocks</li><li>How portability, speed, and accuracy improve productivity</li><li>Innovative software features that strengthen data integrity and audit trails</li><li>How IonicX and TruScan together expand coverage for raw material verification</li></ul><blockquote><p><strong>Presenter: </strong>Meet Linxi Chen</p><p>Linxi Chen is a Field Application Scientist at Thermo Fisher Scientific, specializing in Raman PAT applications. She holds a PhD in Environmental Engineering and has over a decade of industry experience in environmental consulting and analytical instrumentation.</p></blockquote></div></div></div>

Thermo Fisher Scientific: The Perfect Pair for GMP Compliance: IonicX and TruScan for Raw Material Identification and Verification

The Perfect Pair for GMP Compliance: IonicX and TruScan for Raw Material Identification and Verification

Discover how IonicX Portable XRF and TruScan Raman improve GMP raw material identification with fast, compliant, at-line elemental analysis in pharmaceutical

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