<div><div><p><strong>Researchers led by </strong><a target="_blank" rel="noopener noreferrer" href="https://kudova.group.uochb.cz/en"><strong>Eva Kudová</strong></a><strong> from IOCB Prague and Jitka Viktorová from UCT Prague have identified new androstane-based steroid derivatives that can significantly improve the effectiveness of existing antibiotics against multidrug-resistant </strong><i><strong>Staphylococcus aureus</strong></i><strong>.</strong></p></div></div><div><p>The team screened dozens of natural steroidal hormones and synthetic derivatives to see whether they could block bacterial efflux pumps – mechanisms that bacteria use to eject antibiotics and survive treatment. Two compounds emerged as particularly promising: they inhibited efflux pumps and lowered the effective doses of antibiotics such as ciprofloxacin and erythromycin.</p><p>Further analysis showed that one of the compounds also suppressed genes linked to bacterial virulence. Another key finding is that the most active molecules are non-toxic to human immune cells and showed no detectable endocrine activity, suggesting a favorable safety profile.</p><p>These findings support the idea that rationally designed steroid-based molecules could act as antibiotic adjuvants, helping existing drugs overcome bacterial resistance.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg" alt="IOCB Prague: Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg 222w," sizes="100vw" width="1258" height="886"></p><blockquote><h4><strong>Original Article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0223523426001613?via%3Dihub"><strong>Steroidal hormones and neurosteroids - novel therapeutic strategies in bacterial infections: Design, synthesis, and biological evaluation</strong></a></h4><p>Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti-Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová&nbsp;</p><p><i><strong>Eur. J. Med. Chem., 2026, 308, 118716</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.ejmech.2026.118716">https://doi.org/10.1016/j.ejmech.2026.118716</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>The global rise of antibiotic resistance necessitates novel therapeutic strategies for infectious diseases. Inhibition of bacterial efflux pumps, which contribute to multidrug resistance, represents a promising approach to restore or even increase the efficacy of existing antibiotics. Using fluorescence-based ethidium bromide accumulation, broth microdilution, and checkerboard assays, we evaluated 26 endogenous steroidal hormones and neurosteroids, along with 30 synthetic derivatives, for their ability to enhance antibiotic susceptibility in multidrug-resistant <i>Staphylococcus aureus</i>. Structure-activity relationship analysis identified compounds <strong>13</strong> and <strong>16</strong> as lead candidates, exhibiting strong efflux pump inhibition and marked reductions in the minimum inhibitory concentrations of ciprofloxacin and erythromycin. Both compounds showed additive effects in checkerboard assays. Modifications at C-3 (polar substitution) and C-17 (3α,5β-stereochemistry and nonpolar substitution) were essential for potent efflux inhibition and sensitization, although these modifications were not additive when combined. Transcriptome analysis further revealed that compound <strong>13</strong> significantly downregulated <i>S. aureus</i> virulence-associated genes when administered alone or in combination with antibiotics. Cytotoxicity assessment in human peripheral blood mononuclear cells and receptor transactivation assays for estrogen, androgen, and progesterone receptors indicated that the most active derivatives were non-toxic and lacked detectable endocrine activity, suggesting a favorable safety profile. Overall, these findings support the concept that rationally designed andostane-based steroidal scaffolds can function as competitive bacterial efflux pump inhibitors and serve as potential antibiotic adjuvants to mitigate efflux-mediated resistance.</p><h4><strong>Instrumentation</strong></h4><h5><strong>Structural characterization</strong></h5><ul><li><strong>AVANCE III 400 MHz NMR spectrometer</strong> (Bruker)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=LTQ+Orbitrap+XL"><strong>LTQ Orbitrap XL HRMS</strong></a> (Thermo Fisher Scientific, ESI)</li></ul></div>

Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections

<p><strong>Wiley, a global leader in authoritative content and research intelligence for the advancement of scientific discovery, innovation and learning, today announced the release of additional data to its IR and Raman spectral libraries, significantly broadening compound coverage, including materials, polymers, minerals, and others.</strong></p><p>This latest release includes the first installment of Raman spectra from <strong>Wiley's collaboration with the American Museum of Natural History (AMNH), announced earlier this spring</strong>, drawing from one of the world's most important mineral collections.</p><p>“<i>Researchers depend on reference data as a foundation for sound scientific decisions</i>,” said Graeme Whitley, senior director, data science solutions at Wiley. “<i>Continuous updates ensure scientists always have the most current, reliable reference libraries at their fingertips, helping them work faster and make confident decisions when it matters most.</i>”</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Wiley_expands_its_trusted_IR_and_Raman_spectral_library_collections_helping_analytical_labs_make_faster_more_confident_decisions_1a54aaad3a.jpg" alt="Wiley: Wiley expands its trusted IR and Raman spectral library collections—helping analytical labs make faster, more confident decisions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Wiley_Wiley_expands_its_trusted_IR_and_Raman_spectral_library_collections_helping_analytical_labs_make_faster_more_confident_decisions_1a54aaad3a.jpg 245w," sizes="100vw" width="1600" height="856"></p><h4><strong>Summary of updates:</strong></h4><h5><strong>KnowItAll Raman Spectral Library Collection</strong></h5><ul><li><strong>Added: </strong>1,213 spectra</li><li><strong>Now contains: </strong>29K+ spectra</li></ul><p><strong>2 new databases:</strong></p><ul><li>Raman – American Museum of Natural History Minerals – Wiley – 198 spectra</li><li>Raman – Microbiology – 71 spectra</li></ul><p><strong>Spectra added to databases:</strong></p><ul><li>Raman – Consumer Goods &amp; Food-Related Compounds – +104</li><li>Raman – Drugs – +128</li><li>Raman – Materials – +132</li><li>Raman – Minerals – +233</li><li>Raman – Polymers – +5</li><li>Raman – Pigments &amp; Dyes – +342</li></ul><h5><strong>KnowItAll IR Spectral Library Collection</strong></h5><ul><li><strong>Added: </strong>949 spectra</li><li><strong>Now contains: </strong>344K+ spectra</li></ul><p><strong>7 new databases:</strong></p><ul><li>ATR-IR – Clinical – 364 spectra</li><li>ATR-IR – Materials – 10 spectra</li><li>ATR-IR – Pigments &amp; Dyes – 420 spectra</li><li>ATR-IR – Polymers – 21 spectra</li><li>IR – Clinical – 20 spectra</li><li>IR – Materials – 17 spectra</li><li>IR – Microbiology – 75 spectra</li></ul><p><strong>Spectra added to databases:</strong></p><ul><li>IR – Minerals – +5</li><li>IR – Polymers – +17</li></ul><p>Spectral library subscribers automatically gain access to new data, ensuring labs always have the most current, reliable reference libraries to improve analysis, reduce errors, and increase productivity.</p><ul><li>Learn more at: <a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/spectral-databases/"><strong>www.sciencesolutions.wiley.com/spectral-databases</strong></a></li><li>Existing subscribers can access step-by-step guidance on updating their KnowItAll databases at <a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/how-to-update-knowitall-databases"><strong>https://sciencesolutions.wiley.com/how-to-update-knowitall-databases</strong></a></li></ul><h5><strong>Wiley Spectral Databases</strong></h5><p>Wiley's spectral databases are consistently evolving to meet today's research demands, covering multiple spectral techniques across a wide range of applications including polymers, materials, environmental, forensics, toxicology, pharmaceutical, biotech, automotive, aerospace, food, cosmetics, and more. Built on rigorous quality protocols, Wiley delivers industry-leading spectral data and intelligent tools to accelerate analysis and deliver deeper insights — making it the authoritative, trusted source that analytical labs worldwide rely on.&nbsp;</p><h5><strong>About Wiley</strong></h5><p>Wiley (NYSE: WLY) is a global leader in authoritative content and research intelligence for the advancement of scientific discovery, innovation, and learning. With more than 200 years at the center of the scholarly ecosystem, Wiley combines trusted publishing heritage with AI-powered platforms to transform how knowledge is discovered, accessed, and applied. From individual researchers and students to Fortune 500 R&amp;D teams, Wiley enables the transformation of scientific breakthroughs into real-world impact. From knowledge to impact—Wiley is redefining what's possible in science and learning.&nbsp;</p>

Wiley expands its trusted IR and Raman spectral library collections

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 4th May 2026? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by<strong> KNAUER, Shimadzu, </strong>and <strong>Thermo Fisher Scientific!</strong></p><h3><strong>1. KNAUER: Analysis of HPLC fractions by Benchtop NMR</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/knauer_hplc_benchtop_nmr_web_638c60e13c.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Preparative HPLC is essential for isolating compounds and removing impurities in pharmaceutical and chemical industries. Non-destructive, fast analysis is preferred to maintain yield and enable at-line monitoring, avoiding lengthy sample preparation. Recent advancements in benchtop NMR spectrometers (up to 90 MHz) allow quick analysis without deuterated solvents. In this proof-of-concept study we purified a pharmaceutical sample via HPLC and confirmed its identity using benchtop NMR. Fractions (0.7–4.4 mM) were analyzed without preparation, using only 300 µL per sample, ensuring rapid batch control with no product loss. This study demonstrates the seamless integration of HPLC and benchtop NMR.</p><h4><strong>Experimental</strong></h4><h5><strong>Preparative HPLC Method</strong></h5><p>Eluent 20:80 Ethanol/water, isocratic mode, flow rate 12.5 ml/min, detection UV254 nm 2 Hz, 500 µl stacked injection, automated fractionation with threshold function, column temperature ambient, Column Eurospher II 100-10 C18 150x20 mm&nbsp;</p><h5><strong>Analytical HPLC Method</strong></h5><p>Eluent 20:80 Ethanol /water + 0.1% formic acid, isocratic mode, flow rate 1 ml/min, detection UV254 nm 10 Hz, 5 µl partial loop injection, column temperature 25 °C, Column <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1633"><strong>Eurospher II 100-5 C18</strong></a> 150x4 mm&nbsp;</p><h5><strong>NMR Method&nbsp;</strong></h5><p>NMR spectra were acquired on a <strong>Magritek Multi-X Ultra 80 MHz benchtop NMR system</strong> using an adapted WET sequence for solvent suppression. 32 scans were acquired with a repetition time of 15s. WET parameters were adjusted for each sample to yield optimal suppression for all solvent signals. Pooled HPLC fractions were analysed directly without addition of deuterated solvents or other sample preparation.</p><h4><strong>Conclusion</strong></h4><p>This proof-of-concept study successfully shows that HPLC and benchtop NMR can be used together easily. Samples can be analyzed non-destructively within minutes and without any further preparation due to highly efficient suppression of multiple solvent signals. Compounds can be identified without the need of reference standards. This study shows that NMR can provide a valid compatible alternative detector for HPLC fraction analysis.</p><h3><strong>2. Shimadzu: DIC Analysis in High-Speed Tensile Test of CFRP</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00854_en_fb6c68b858.pdf"><strong>Full PDF for download</strong></a></li></ul><h4><strong>User Benefits</strong></h4><ul><li>The <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=HPV-X3"><strong>HPV-X3</strong></a> is suitable for observation of fracture of brittle materials such as CFRP because it has a maximum recording speed (framerate) of 20 Mfps.&nbsp;</li><li>The HPV-X3 provides improved DIC analysis performance, as its resolution is 3 times higher than that of the conventional highspeed video camera.</li><li>The HITS-TX impact testing machine enables testing at a maximum speed of 20 m/s</li></ul><p>Carbon fiber reinforced plastic (CFRP) is a material with particularly high specific strength, even among composite materials, and used in various applications such as transport aircraft, taking advantage of its excellent mechanical properties. Because CFRP consists of carbon fibers and resin, it shows complex fracture behavior, and brittle fracture progresses from damage as the point of origin. Since fracture proceeds instantaneously, a high-speed video camera with a high recording speed is necessary when observing fracture of CFRP. One method for measuring strain in a high-speed tensile test is Digital Image Correlation (DIC), which visualizes the distribution of strain at the specimen surface by comparing a random pattern on the specimen surface before and after deformation. Thus, a high-speed video camera with a high resolution is also necessary in order to improve the DIC performance.&nbsp;</p><p>Newly-developed <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=HPV-X3"><strong>Hyper Vision HPV-X3 high-speed video camera</strong></a> is extremely well-suited to DIC analysis of CFRP, as its recording speed is 2 timesfaster and itsresolution is 3 times higher than the conventional HPV-X2. In this experiment, a high-speed tensile test of CFRP was conducted using a HITS-TX high-speed tensile testing machine. The condition of the test was observed with the new HPV-X3, and the strain distribution of the CFRP test piece was clarified by a DIC analysis of the recorded images.</p><h4><strong>Test Piece and Test Instruments</strong></h4><p>A CFRP quasi-isotropic laminate was used as the test piece. Aluminum foil was glued to the back side of the test piece, and the timing when the electrical conduction of the aluminum foil was interrupted by fracture of the test piece was used as the trigger for video recording. The HITS-TX was used in the highspeed tensile test. The HPV-X3 was used in fracture observation, and for comparison, the fracture process was also recorded with the conventional HPV-X2 camera. Fig. 1 shows the condition of the test, and Table 1 and Table 2 show the equipment composition and test conditions, respectively.</p><h4><strong>DIC Analysis Results in High-Speed Tensile Test</strong></h4><p>Fig. 3 shows the DIC analysis results in the high-speed tensile test. The color bar was set to display the color purple at around 0 % strain and red at around 2 % strain. Fig. 3 (1) is an image at the start of the test. After the test started, strain in the 45˚ direction concentrated from the lower left to the right central area, as can be seen in Fig. 3 (2). Then, as the test progressed, a condition of increasing strain in the initial area of strain concentration can be seen in Fig. 3 (3) to (9). A crack occurs in Fig. 3 (10), and strain decreases in the crack area. Following this, the test piece fractured completely in Fig. 3 (11) and (12), and a decrease in the strain in the area around the crack could be observed.</p><h4><strong>Conclusion</strong></h4><p>A high-speed tensile test of CFRP was conducted using a HITSTX high-speed tensile testing machine and the newlydeveloped HPV-X3 high-speed video camera, and the strain distribution at the time of test piece fracture was visualized by a DIC analysis. Because the HPV-X3 offers a 3 times higher resolution and a 2 times faster recording speed than the conventional device, it is a suitable high-speed video camera for fracture observation and DIC analysis in impact tests of brittle materials such as CFRP. The measurement system introduced in this Application News article is a useful tool for the development of composite materials.</p><h3><strong>3. Thermo Fisher Scientific: Polymer analysis using femtosecond-laser-ablation depth profiling</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/xps_depth_profiling_polymer_analysis_appnote_c731e2e7e0.pdf"><strong>Full PDF for download</strong></a></li></ul><p>X-ray photoelectron spectroscopy (XPS) is an essential polymer characterization technique that can identify not just the elemental composition but also the chemical state of polymer samples. This allows the chemical bonding environment to be deduced and, as a result, the polymer species can be identified. This can all be achieved with minimal sample preparation thanks to the built-in charge-compensation systems found in XPS instruments, which simplify the analysis of insulating samples.&nbsp;</p><p>The introduction of gas cluster ion sources (GCIS), such as the Thermo Scientific™ MAGCIS Dual Mode Ion Source, marked a major step forward in polymer analysis. Unlike monatomic ion beams, GCIS are able to clean surface contamination, and etch away layers of sample material, without damaging the underlying chemistry of organic samples such as polymers. As a result, GCIS has allowed chemical-damage-free analysis to reach micrometers below the sample surface. This depth of analysis can be highly time consuming, however, and total experimental times can range from multiple hours to days.&nbsp;</p><p>The addition of femtosecond-laser-ablation (fs-LA) technology is alleviating this, allowing for XPS spectra to be collected from a depth of tens of µm into a sample in a matter of minutes. This is because the laser ablation occurs on the femtosecond timescale (i.e., nearly instantaneously), effectively eliminating the material removal time of the depth profile. Additionally, unlike other material removal techniques such as atomic ion beam impact (where a collision cascade effect is induced to remove surface material) fs-LA is capable of removing material without chemical damage due to the ultrashort columbic explosion process used to eject the material. The resulting profile is therefore representative of the actual sample chemistry.</p><h4><strong>Paint layer analysis&nbsp;</strong></h4><p>In traditional ion-beam depth profiling, analytical depths are generally limited to several hundred nanometers, up to a micrometer. This means that it is extremely difficult to analyze the interfaces between multiple micrometer-thick layers in a sample without the use of additional techniques such as ultralow-angle microtome (ULAM) sectioning.¹ The introduction of fs-LA technology, however, allows micrometers of material to be removed rapidly, enabling the characterization of layered samples.&nbsp;</p><p>As an example, a multi-layer paint specimen (provided by the University of Surrey, UK) was depth profiled using the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2206"><strong>Thermo Scientific™ Hypulse™ Surface Analysis System</strong></a>. The sample consists of an aluminum substrate coated with a 5 µm layer of polyurethane primer followed by a 25 µm PMMA/PVdF topcoat.</p><p>The depth profile was generated using a 1,030 nm laser with an energy of 250 µJ for ablation, along with a 30 µm X-ray spot size for data collection. XPS spectra were collected after each ablation cycle to obtain an atomic concentration plot through both paint layers into the substrate material (Figure 2).&nbsp;</p><p>5 distinct regions throughout the analyzed depth can be seen. The top surface of the sample consists of an adventitious layer of carbon contamination, which is commonly found on samples exposed to air. The second layer is the 25 µm PVdF/PMMA topcoat, and the third layer is the PU primer. Between the primer and the final aluminum substrate layer, a clear increase in adventitious carbon is observed, similar to the signal seen at the top sample surface. This suggests residual carbon contamination was present on the substrate before the paint layers were applied.&nbsp;</p><p>It would take hours to produce the XPS depth profile shown in Figure 2 using traditional ion beam approaches. Even with the use of a monatomic beam with a high energy per atom, the length of the analysis could still be just as long, depending on the sample material, and would sacrifice the chemistry of any organic/polymeric materials. The combination of fs-LA depth profiling and snapshot XPS acquisition, meanwhile, can reduce the experimental time to less than 20 minutes without inducing any chemical damage to the sample surface.</p><h4><strong>Summary&nbsp;</strong></h4><p>One of the most critical aspects of successful polymer XPS depth profiling is the preservation of the sample chemistry during material removal. Additionally, experiment time can be a significant limiting factor, and thicker samples could lead to reduced time for repeat experiments. The femtosecond laser ablation capabilities of the Hypulse Surface Analysis System provide fast, efficient, and chemical-damage-free material removal from a sample surface, enabling analysis multiple micrometers into a sample. This means that hidden sample interfaces, that were largely inaccessible before, can now be analyzed easily and efficiently</p>

News from LabRulezICPMS Library - Week 19, 2026

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Application

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Science and research

Software

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

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LECO Cold Mounting Castable Mounts for Metallographic Sample Preparation

Cold mounting materials from LECO include polyester, acrylic, and epoxy resins designed for porous, brittle, or heat-sensitive samples, ensuring easy handling and excellent edge integrity.

<p><strong>Cold mounting—also known as castable mounting—is a metallographic sample preparation technique that uses liquid mounting media to fully encase a specimen. Common mounting materials include polyester, acrylic, and epoxy resins. After the sample is positioned in a mold, a catalyst is added to initiate curing and hardening of the mounting compound.</strong></p><p>This mounting approach is especially suitable for laboratories with lower throughput but a high diversity of samples. Cold mounting is often the preferred—or required—method for porous, brittle, irregularly shaped, or heat-sensitive specimens that cannot withstand the temperature and pressure of hot mounting. Once cured, the mounted samples are easier to handle during grinding, polishing, and analysis, while preserving edge integrity and microstructural features.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_Zalevaci_kelimky_pro_pripravu_metalografickych_vzorku_2_b63c61bea7.jpg" alt="LECO: Cold Mounting Castable Mounts for Metallographic Sample Preparation" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_Zalevaci_kelimky_pro_pripravu_metalografickych_vzorku_2_b63c61bea7.jpg 178w," sizes="100vw" width="884" height="776"></p><h4><strong>Cold Mounting Media Options</strong></h4><p>We offer a complete range of cold mounting materials to support different laboratory needs and sample types:</p><ul><li><strong>Polyester Resins</strong><br>Polyesters are an economical solution for laboratories processing lower sample volumes and where edge retention is not a primary concern. They allow for fast and straightforward mounting and are well suited for routine applications requiring quick turnaround.</li><li><strong>Acrylic Resins</strong><br>Acrylics cure faster than most other castable mounting materials and offer good abrasion resistance with fair edge retention. They are commonly used when multiple mounts are required and hot mounting is impractical. When used with a pressure chamber, such as the LECOMAT Pressure Vessel, acrylics can produce semi-clear mounts.</li><li><strong>Epoxy Resins</strong><br>Epoxies provide hard, chemically resistant, and abrasion-resistant mounts with excellent edge retention. Although they generally require longer curing times, they offer superior flow characteristics and minimal shrinkage. Long-cure epoxies are ideal for challenging samples and can be vacuum-impregnated to remove trapped air and fill internal voids. Quick-cure epoxy formulations are also available, curing in less than one hour.</li></ul><h4><strong>Available Products (Models)</strong></h4><ul><li><strong>Castolite</strong> – Polyester</li><li><strong>LECOSET 7000</strong> – Polyester</li><li><strong>LECOSET 7008</strong> – Acrylic</li><li><strong>Quick-Cure Epoxies</strong></li><li><strong>Long-Cure Epoxies</strong></li></ul><h4><strong>Accessories and Options</strong></h4><ul><li>Ring molds</li><li>Silicone rubber molds</li><li>Mold cups</li><li>General mounting supplies</li><li><strong>LECOMAT Pressure Vessel</strong> for pressure impregnation and improved clarity</li></ul>

LECO DS20 Hand Grinder for Metallographic Polishing

The LECO DS20 is a compact, cost-effective benchtop hand grinder with sloped PVC platens, water flush, and integrated abrasive storage for efficient metallographic sample preparation.

<p><strong>The LECO DS20 Hand Grinder is a cost-effective, benchtop solution designed to simplify and streamline manual grinding during metallographic sample preparation. With a straightforward, durable design and practical workflow enhancements, the DS20 is ideal for laboratories seeking reliable surface preparation without unnecessary complexity.</strong></p><p>Engineered for ease of use and consistent results, the DS20 provides stable grinding surfaces, efficient debris removal, and integrated storage to support clean, repeatable operation in everyday lab environments.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_DS_20_Rucni_bruska_pro_metalograficke_lesteni_aa51980ffb.jpg" alt="LECO: DS20 Hand Grinder for Metallographic Polishing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_DS_20_Rucni_bruska_pro_metalograficke_lesteni_aa51980ffb.jpg 213w," sizes="100vw" width="1031" height="757"></p><h4><strong>Key Features</strong></h4><ul><li>Four sloped PVC platens providing flat, non-corroding, and well-drained grinding surfaces</li><li>Individual clamps and guides to keep abrasive paper flat during grinding</li><li>Built-in continuous water flush for effective removal of abrasive swarf</li><li>Closed storage compartment to keep abrasive rolls clean, dry, and readily accessible</li><li>Convenient abrasive paper roll system for fast changes</li><li>Supplied with silicon carbide abrasive rolls in <strong>240, 320, 400, and 600 grit</strong></li></ul><p>The DS20 is a practical choice for laboratories that value simplicity, reliability, and efficiency in manual grinding applications.</p>

LECO LM Series Microindentation Hardness Testing Systems

The LECO LM Series offers flexible microindentation hardness testing from 1 gf to 2000 gf, with compact designs, multiple turret options, and analog or digital models for labs and production sites.

<p><strong>The LECO LM Series Microindentation Hardness Testing Systems are designed to meet the needs of both production environments and research laboratories. With a broad range of configurations—from economical analog models to advanced digital systems—the LM Series offers flexible solutions that can be tailored to your application requirements and budget.</strong></p><p>These systems support precise microindentation testing across a wide load range and are suitable for detailed hardness evaluation of thin layers, coatings, and small or delicate specimens. Their compact footprint, flexible turret configurations, and wide selection of objectives make the LM Series a versatile and reliable choice for modern hardness testing workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_LM_Stanoveni_mikrotvrdosti_12e8c38050.jpg" alt="LECO: LM Series Microindentation Hardness Testing Systems" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_LM_Stanoveni_mikrotvrdosti_12e8c38050.jpg 235w," sizes="100vw" width="416" height="276"></p><h4><strong>Key Features</strong></h4><ul><li>Load range from <strong>1 gf to 2000 gf</strong> for broad application coverage</li><li>Manual or automatic turret options</li><li>Wide selection of objectives from <strong>2.5X to 100X</strong></li><li>Turret configuration supporting up to <strong>four objectives and two indenters</strong></li><li>Compact design to save valuable laboratory space</li></ul><h4><strong>Applications</strong></h4><ul><li>Coating and surface layer hardness testing (e.g., nitrided layers)</li><li>Hardness profiling across material layers</li><li>Thin specimen analysis</li><li>Weld and heat-affected zone evaluation</li></ul><h4><strong>Available Models</strong></h4><ul><li><strong>LM110 Series</strong> – Economical solution for low-volume laboratories</li><li><strong>LM310 Series</strong> – Digital measurement with automatic hardness calculations</li><li><strong>LM810 Series</strong> – Digital measurement, hardness calculations, conversions, and statistical analysis</li><li><strong>LM248AT Dual Indenter</strong> – Digital measurements with conversions and statistical hardness data using dual indenters</li></ul>

LECO LCB3100 Brinell Load Cell Hardness Tester

LCB3100 is a Brinell load cell hardness tester with touch-pad control, automatic load application, and selectable loads, delivering accurate, repeatable hardness testing without mechanical weights.

<p><strong>The LECO LCB3100 is a modern Brinell hardness tester that uses advanced load cell technology to deliver accurate, repeatable, and user-friendly hardness measurements. Designed to simplify operation and reduce the risk of operator error, the LCB3100 eliminates traditional mechanical weights and adjustment knobs in favor of an intuitive electronic control system.</strong></p><p>Hardness testing is performed through a simple touch-pad operation panel, where the user selects the desired test load and dwell time. The instrument then automatically applies, holds, and removes the load using direct load-cell feedback, ensuring precise force control and consistent results. Its rugged construction and enclosed load cell make the LCB3100 well suited for routine laboratory use as well as demanding industrial environments.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_LCB_3100_Tvrdomer_Brinell_se_snimacem_zatizeni_9cb81e86c5.jpg" alt="LECO: LCB3100 Brinell Load Cell Hardness Tester" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_LCB_3100_Tvrdomer_Brinell_se_snimacem_zatizeni_9cb81e86c5.jpg 196w," sizes="100vw" width="1723" height="1371"></p><h4><strong>Key Features</strong></h4><ul><li>Twelve selectable Brinell load options for flexible testing</li><li>Direct load method with real-time load-cell feedback</li><li>Automatic load application, holding, and removal</li><li>Selectable dwell times to meet different testing standards</li><li>Heavy-duty cast housing with enclosed load cell for long-term stability</li><li>User-friendly touch-pad operation panel that simplifies setup and minimizes errors</li></ul>

LECO AMH55 Software for Automated Hardness Testing

AMH55 software enables automated, semi-automated, and manual hardness testing with advanced image recognition, flexible reporting, and Cornerstone® control for accurate microindentation and Vickers analysis.

<p><strong>LECO’s AMH55 software is designed to maximize productivity and measurement confidence in microindentation and Macro/Vickers hardness testing. By introducing LECO’s Cornerstone® software platform to hardness testing systems, AMH55 delivers advanced automation, intuitive operation, and flexible reporting for a wide range of applications and surface conditions.</strong></p><p>AMH55 supports fully automatic, semi-automatic, and lite configurations, enabling laboratories to tailor their hardness testing workflow to specific needs while maintaining high accuracy and repeatability. Advanced image-recognition technology ensures reliable evaluation even on challenging sample surfaces, making AMH55 a powerful solution for modern hardness testing environments.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_AMH_55_Software_pro_automaticke_testovani_tvrdosti_2_9317816b67.jpg" alt="LECO: AMH55 Software for Automated Hardness Testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_AMH_55_Software_pro_automaticke_testovani_tvrdosti_2_9317816b67.jpg 172w," sizes="100vw" width="838" height="761"></p><h4><strong>Key Features</strong></h4><ul><li>Advanced image-recognition technology for analysis beyond simple threshold-based methods</li><li>Reliable measurement on scratched, etched, uneven, or variably illuminated surfaces</li><li>Cornerstone® software with optional touch-screen interface for full control of methods, calibrations, analysis parameters, and reporting</li><li>Flexible configurations to support automatic, semi-automatic, or manual workflows</li><li>Customizable data reporting to match laboratory and application requirements</li></ul><h4><strong>Configuration Options</strong></h4><h5><strong>AMH55 Automatic</strong></h5><ul><li>Fully automatic alignment and parfocal tools</li><li>Automatic pixel calibration and shading correction</li><li>Optional Advanced Analysis Module for color-contoured hardness mapping</li><li>Fully customizable reporting</li><li>Motorized X, Y, and Z axis movement</li></ul><h5><strong>AMH55 Semi-Automatic</strong></h5><ul><li>Automatic indent location and analysis</li><li>Automatic alignment and pixel calibration</li><li>Customizable reporting</li><li>Motorized X and Y axes</li><li>Manual focus</li></ul><h5><strong>AMH55 Lite</strong></h5><ul><li>Automatic indent detection and analysis within a single field of view</li><li>Automatic pixel calibration</li><li>Customizable reporting</li><li>Manual X, Y, and Z axes</li><li>Optional digital stage micrometers for position tracking</li><li>Upgradeable to AMH55 Semi-Automatic or Automatic</li></ul><h4><strong>Compatible Models</strong></h4><ul><li><strong>AMH55 with LM110 Series</strong> microindentation systems</li><li><strong>AMH55 with LM248 Series</strong> microindentation systems</li><li><strong>AMH55 with LV110/LV810 Series</strong> Macro Vickers hardness testers</li></ul>

LECO LR/LCR Series Rockwell-Type Hardness Testing System

LECO LR/LCR Series Rockwell hardness testers deliver accurate, repeatable results for high-volume labs, offering Rockwell, Superficial, and automated load-cell testing options.

<p><strong>The LECO LR/LCR Series Rockwell-type hardness testing systems are designed for high-throughput laboratories that require reliable, repeatable, and efficient hardness measurements. Built for routine industrial and quality control environments, these instruments combine robust mechanical design with intuitive operation to ensure consistent results, even under heavy workloads.</strong></p><p>With a range of configurations available—including analog, digital, and fully automated load-cell-based systems—the LR/LCR Series can be precisely matched to your testing requirements. Whether performing standard Rockwell, Superficial Rockwell, or advanced automated testing, these systems deliver confidence, ease of use, and long-term stability.</p><h4><strong>Key Features</strong></h4><ul><li>User-friendly operation panel for simple and efficient testing</li><li>Clear OK/NG indicators for rapid pass/fail evaluation</li><li>Automatic measuring mode to improve repeatability and reduce operator influence</li><li>Integrated hardness conversion scales displayed directly on the operation panel</li><li>Free loads and optional light-load Brinell testing available on the LCR500 model</li></ul><h4><strong>Available Models</strong></h4><h5><strong>LR110 Series</strong></h5><ul><li>Rockwell-only or Superficial-only testing</li></ul><h5><strong>LR310 Series</strong></h5><ul><li>Supports both Rockwell and Superficial Rockwell testing</li></ul><h5><strong>LCR500</strong></h5><ul><li>Fully automated load-cell Rockwell-type hardness tester</li><li>Supports free loads and optional light-load Brinell testing</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_LR_LCR_Tvrdomery_Rockwell_3_aaf47a1ae5.jpg" alt="LECO: LR/LCR Series Rockwell-Type Hardness Testing System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_LR_LCR_Tvrdomery_Rockwell_3_aaf47a1ae5.jpg 231w," sizes="100vw" width="1613" height="1091"></p>

LECO LV Series Macro-Vickers Hardness Testing System

LECO LV Series Macro-Vickers hardness testers provide affordable, flexible hardness testing for large samples, offering wide load ranges, multiple objectives, and upgrade options for demanding applications.

<p><strong>The LECO LV Series Macro-Vickers hardness testing systems provide an affordable and versatile solution for hardness testing of larger and heavier samples commonly encountered in welding, metallurgical, and industrial applications. Designed for reliability and flexibility, the LV Series supports both routine and more demanding hardness measurements across a wide load range.</strong></p><p>Available in analog and digital configurations, the LV Series can be tailored to laboratory needs, with upgrade paths toward more automated operation. This modular approach allows laboratories to start with a basic configuration and expand capabilities as testing requirements evolve.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_LV_Stanoveni_makrotvrdosti_2_c6b6bb0ddb.jpg" alt="LECO: LV Series Macro-Vickers Hardness Testing System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_LV_Stanoveni_makrotvrdosti_2_c6b6bb0ddb.jpg 220w," sizes="100vw" width="1795" height="1269"></p><h4><strong>Key Features</strong></h4><ul><li>Turret accommodating up to three objectives for flexible inspection and measurement</li><li>Improved, finer-pitched focusing mechanism compared to previous generations</li><li>Wide load range with eight load steps from 0.3 kgf up to 50 kgf</li><li>Broad selection of objectives ranging from 2.5× to 100×</li><li>Optional light-load Brinell testing on the LV810 (custom load set required)</li></ul><h4><strong>Available Models</strong></h4><h5><strong>LV110 Series</strong></h5><ul><li>Economical solution for low-volume laboratories</li><li>Supports a combination of micro and macro loads</li></ul><h5><strong>LV810 Series</strong></h5><ul><li>Supports a combination of micro and macro loads</li><li>Optional light-load Brinell testing capability</li></ul>

LECO BG Series Belt Grinder for Metallographic Sample Polishing

The LECO BG Series belt grinders provide durable, safe, and flexible wet or dry grinding for metallographic and spectrographic sample preparation in single- or dual-belt configurations.

<p><strong>The BG Series belt grinders are designed to deliver durable, safe, and convenient grinding performance for metallographic and spectrographic sample preparation. Available in both single-belt and dual-belt configurations, the BG Series supports efficient material removal while maintaining operator safety and consistent results.</strong></p><p>By combining the grinder with an optional dust collector or a recirculating system, the BG Series can be configured for either dry or wet grinding, making it a flexible solution for a wide range of laboratory workflows. Its robust construction and straightforward operation make it well suited for routine use in production, quality control, and research laboratories.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_BG_Pasova_bruska_pro_lesteni_metalografickych_vzorku_5_adfd9793f2.jpg" alt="LECO: BG Series Belt Grinder for Metallographic Sample Polishing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_BG_Pasova_bruska_pro_lesteni_metalografickych_vzorku_5_adfd9793f2.jpg 245w," sizes="100vw" width="1303" height="691"></p><h4><strong>Key Features</strong></h4><ul><li>Heavy-duty 1 HP (0.75 kW) motor for reliable and consistent grinding performance</li><li>Enclosed belt tension mechanism that prevents belt slippage and improves coolant and debris drainage</li><li>Integrated safety guards to maximize operator protection during operation</li><li>Corrosion-resistant aluminum external surfaces for long service life in laboratory environments</li></ul><h4><strong>Available Models</strong></h4><ul><li><strong>BG30</strong> – Single belt drive configuration</li><li><strong>BG32</strong> – Dual belt drive configuration</li></ul>

LECO VX4 Compact Inverted Microscope

LECO VX4 is a compact inverted metallurgical microscope delivering high-resolution imaging, polarized light, and advanced optics for precise microstructural analysis in materials and educational labs.

<p><strong>The LECO VX Series metallurgical microscopes are advanced microscopy solutions designed for materials laboratories that require high-resolution imaging, reliable performance, and consistent metallographic results. Built with a strong focus on precision, repeatability, and ease of operation, the VX Series supports a wide range of applications across automotive, aerospace, academic research, and quality assurance laboratories.</strong></p><p>From routine microstructural evaluation to detailed failure investigations, the VX Series delivers the optical quality and robustness professionals expect. Integrated digital imaging systems and compatibility with LECO’s analytical software tools ensure efficient workflows, clear documentation, and scalable solutions for laboratories of all sizes. The <strong>VX3, VX4, and VX5 models</strong> allow laboratories to select the configuration that best matches their technical and budgetary requirements.</p><h4><strong>Why the VX Series Is Ideal for Metallography</strong></h4><p>LECO metallographic microscopes play a critical role in examining the microstructure of metals and alloys. This essential step in metallographic analysis enables detailed evaluation of grain size, phase distribution, and structural features, helping users identify defects and assess material quality. The high level of detail provided by the VX Series supports quality control, failure analysis, and materials inspection across a broad range of industries.</p><h4><strong>LECO VX4 Compact Inverted Microscope</strong></h4><p>The LECO VX4 is a compact yet powerful inverted microscope designed for metallurgical and educational laboratories. It features a bright LED polarized light source suitable for both mounted and unmounted samples, delivering excellent contrast and image clarity. Engineered for strong optical performance and intuitive operation, the VX4 combines modern imaging technology with automated functionality to support demanding material investigations.</p><p>The VX4 is well suited for microstructural analysis such as <strong>grain size determination</strong>, <strong>pearlite/ferrite evaluation</strong>, <strong>nodularity assessment, dendrite arm spacing measurements</strong>, and <strong>advanced failure analysis</strong>. Full compatibility with LECO’s analytical software enhances efficiency in image capture, analysis, data management, and reporting, making the VX4 a practical and future-ready solution for metallography and materials characterization.</p><h4><strong>Key Features of the VX4</strong></h4><ul><li>Variable LED light source with built-in lever condenser, including field and aperture diaphragms</li><li>Trinocular viewing head with 10× / 22 mm adjustable focusing eyepieces</li><li>Revolving nosepiece accommodating up to five objectives</li><li>Standard objectives: 5×, 10×, 20×, and 50×</li><li>Optional 100× objective available</li><li>Brightfield observation with 360° polarizer/analyzer</li><li>Coaxial coarse and fine focus controls for precise adjustment</li><li>Ergonomic mechanical XY stage for smooth sample positioning</li></ul>

LECO SX100 Benchtop Metallographic Saw

SX100M benchtop sectioning machine delivers precise and safe cutting of metals, carbides, composites, and electronic components with a powerful motor and compact, ergonomic design.

<p><strong>The SX100M is an efficient benchtop sectioning machine designed to handle a wide range of materials and applications with precision and reliability. It is an ideal solution for sectioning bar stock, castings, fasteners, tungsten carbide, composite materials, electronic components, weldments, and more. With an approximate maximum sample cut diameter of 2.75 inches, the SX100M delivers dependable performance in a compact laboratory-friendly footprint.</strong></p><p>Equipped with a powerful 3 HP (2.2 kW) motor and a 10-inch (250 mm) blade capacity, the SX100M ensures consistent cutting results even for demanding materials. An integrated electronic mechanical braking system enhances operator safety and improves workflow efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_SX_100_Stolni_metalograficka_pila_83b2b057c2.jpg" alt="LECO: SX100 Benchtop Metallographic Saw" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_SX_100_Stolni_metalograficka_pila_83b2b057c2.jpg 158w," sizes="100vw" width="1185" height="1175"></p><h4><strong>Key Features</strong></h4><ul><li>Radial arm design for manual operation with an ergonomic, user-friendly working height</li><li>Base aluminum casting combined with a steel hood for durability and long service life</li><li>Integrated safety lock to protect the operator during sectioning</li><li>Built-in 6.6-gallon (25 L) recirculating coolant tank</li><li>Sloped interior floor for efficient coolant flow and reduced debris accumulation</li><li>Electronic mechanical braking system for rapid and safe blade stopping</li></ul><h4><strong>Models</strong></h4><h5><strong>SX100M</strong></h5><ul><li>Power supply options: 230 V or 400 V</li></ul>

Products from LECO - page 2

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