<div><div><p><strong>Researchers led by </strong><a target="_blank" rel="noopener noreferrer" href="https://kudova.group.uochb.cz/en"><strong>Eva Kudová</strong></a><strong> from IOCB Prague and Jitka Viktorová from UCT Prague have identified new androstane-based steroid derivatives that can significantly improve the effectiveness of existing antibiotics against multidrug-resistant </strong><i><strong>Staphylococcus aureus</strong></i><strong>.</strong></p></div></div><div><p>The team screened dozens of natural steroidal hormones and synthetic derivatives to see whether they could block bacterial efflux pumps – mechanisms that bacteria use to eject antibiotics and survive treatment. Two compounds emerged as particularly promising: they inhibited efflux pumps and lowered the effective doses of antibiotics such as ciprofloxacin and erythromycin.</p><p>Further analysis showed that one of the compounds also suppressed genes linked to bacterial virulence. Another key finding is that the most active molecules are non-toxic to human immune cells and showed no detectable endocrine activity, suggesting a favorable safety profile.</p><p>These findings support the idea that rationally designed steroid-based molecules could act as antibiotic adjuvants, helping existing drugs overcome bacterial resistance.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg" alt="IOCB Prague: Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg 222w," sizes="100vw" width="1258" height="886"></p><blockquote><h4><strong>Original Article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0223523426001613?via%3Dihub"><strong>Steroidal hormones and neurosteroids - novel therapeutic strategies in bacterial infections: Design, synthesis, and biological evaluation</strong></a></h4><p>Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti-Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová&nbsp;</p><p><i><strong>Eur. J. Med. Chem., 2026, 308, 118716</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.ejmech.2026.118716">https://doi.org/10.1016/j.ejmech.2026.118716</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>The global rise of antibiotic resistance necessitates novel therapeutic strategies for infectious diseases. Inhibition of bacterial efflux pumps, which contribute to multidrug resistance, represents a promising approach to restore or even increase the efficacy of existing antibiotics. Using fluorescence-based ethidium bromide accumulation, broth microdilution, and checkerboard assays, we evaluated 26 endogenous steroidal hormones and neurosteroids, along with 30 synthetic derivatives, for their ability to enhance antibiotic susceptibility in multidrug-resistant <i>Staphylococcus aureus</i>. Structure-activity relationship analysis identified compounds <strong>13</strong> and <strong>16</strong> as lead candidates, exhibiting strong efflux pump inhibition and marked reductions in the minimum inhibitory concentrations of ciprofloxacin and erythromycin. Both compounds showed additive effects in checkerboard assays. Modifications at C-3 (polar substitution) and C-17 (3α,5β-stereochemistry and nonpolar substitution) were essential for potent efflux inhibition and sensitization, although these modifications were not additive when combined. Transcriptome analysis further revealed that compound <strong>13</strong> significantly downregulated <i>S. aureus</i> virulence-associated genes when administered alone or in combination with antibiotics. Cytotoxicity assessment in human peripheral blood mononuclear cells and receptor transactivation assays for estrogen, androgen, and progesterone receptors indicated that the most active derivatives were non-toxic and lacked detectable endocrine activity, suggesting a favorable safety profile. Overall, these findings support the concept that rationally designed andostane-based steroidal scaffolds can function as competitive bacterial efflux pump inhibitors and serve as potential antibiotic adjuvants to mitigate efflux-mediated resistance.</p><h4><strong>Instrumentation</strong></h4><h5><strong>Structural characterization</strong></h5><ul><li><strong>AVANCE III 400 MHz NMR spectrometer</strong> (Bruker)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=LTQ+Orbitrap+XL"><strong>LTQ Orbitrap XL HRMS</strong></a> (Thermo Fisher Scientific, ESI)</li></ul></div>

Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections

<p><strong>Wiley, a global leader in authoritative content and research intelligence for the advancement of scientific discovery, innovation and learning, today announced the release of additional data to its IR and Raman spectral libraries, significantly broadening compound coverage, including materials, polymers, minerals, and others.</strong></p><p>This latest release includes the first installment of Raman spectra from <strong>Wiley's collaboration with the American Museum of Natural History (AMNH), announced earlier this spring</strong>, drawing from one of the world's most important mineral collections.</p><p>“<i>Researchers depend on reference data as a foundation for sound scientific decisions</i>,” said Graeme Whitley, senior director, data science solutions at Wiley. “<i>Continuous updates ensure scientists always have the most current, reliable reference libraries at their fingertips, helping them work faster and make confident decisions when it matters most.</i>”</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Wiley_expands_its_trusted_IR_and_Raman_spectral_library_collections_helping_analytical_labs_make_faster_more_confident_decisions_1a54aaad3a.jpg" alt="Wiley: Wiley expands its trusted IR and Raman spectral library collections—helping analytical labs make faster, more confident decisions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Wiley_Wiley_expands_its_trusted_IR_and_Raman_spectral_library_collections_helping_analytical_labs_make_faster_more_confident_decisions_1a54aaad3a.jpg 245w," sizes="100vw" width="1600" height="856"></p><h4><strong>Summary of updates:</strong></h4><h5><strong>KnowItAll Raman Spectral Library Collection</strong></h5><ul><li><strong>Added: </strong>1,213 spectra</li><li><strong>Now contains: </strong>29K+ spectra</li></ul><p><strong>2 new databases:</strong></p><ul><li>Raman – American Museum of Natural History Minerals – Wiley – 198 spectra</li><li>Raman – Microbiology – 71 spectra</li></ul><p><strong>Spectra added to databases:</strong></p><ul><li>Raman – Consumer Goods &amp; Food-Related Compounds – +104</li><li>Raman – Drugs – +128</li><li>Raman – Materials – +132</li><li>Raman – Minerals – +233</li><li>Raman – Polymers – +5</li><li>Raman – Pigments &amp; Dyes – +342</li></ul><h5><strong>KnowItAll IR Spectral Library Collection</strong></h5><ul><li><strong>Added: </strong>949 spectra</li><li><strong>Now contains: </strong>344K+ spectra</li></ul><p><strong>7 new databases:</strong></p><ul><li>ATR-IR – Clinical – 364 spectra</li><li>ATR-IR – Materials – 10 spectra</li><li>ATR-IR – Pigments &amp; Dyes – 420 spectra</li><li>ATR-IR – Polymers – 21 spectra</li><li>IR – Clinical – 20 spectra</li><li>IR – Materials – 17 spectra</li><li>IR – Microbiology – 75 spectra</li></ul><p><strong>Spectra added to databases:</strong></p><ul><li>IR – Minerals – +5</li><li>IR – Polymers – +17</li></ul><p>Spectral library subscribers automatically gain access to new data, ensuring labs always have the most current, reliable reference libraries to improve analysis, reduce errors, and increase productivity.</p><ul><li>Learn more at: <a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/spectral-databases/"><strong>www.sciencesolutions.wiley.com/spectral-databases</strong></a></li><li>Existing subscribers can access step-by-step guidance on updating their KnowItAll databases at <a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/how-to-update-knowitall-databases"><strong>https://sciencesolutions.wiley.com/how-to-update-knowitall-databases</strong></a></li></ul><h5><strong>Wiley Spectral Databases</strong></h5><p>Wiley's spectral databases are consistently evolving to meet today's research demands, covering multiple spectral techniques across a wide range of applications including polymers, materials, environmental, forensics, toxicology, pharmaceutical, biotech, automotive, aerospace, food, cosmetics, and more. Built on rigorous quality protocols, Wiley delivers industry-leading spectral data and intelligent tools to accelerate analysis and deliver deeper insights — making it the authoritative, trusted source that analytical labs worldwide rely on.&nbsp;</p><h5><strong>About Wiley</strong></h5><p>Wiley (NYSE: WLY) is a global leader in authoritative content and research intelligence for the advancement of scientific discovery, innovation, and learning. With more than 200 years at the center of the scholarly ecosystem, Wiley combines trusted publishing heritage with AI-powered platforms to transform how knowledge is discovered, accessed, and applied. From individual researchers and students to Fortune 500 R&amp;D teams, Wiley enables the transformation of scientific breakthroughs into real-world impact. From knowledge to impact—Wiley is redefining what's possible in science and learning.&nbsp;</p>

Wiley expands its trusted IR and Raman spectral library collections

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 4th May 2026? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by<strong> KNAUER, Shimadzu, </strong>and <strong>Thermo Fisher Scientific!</strong></p><h3><strong>1. KNAUER: Analysis of HPLC fractions by Benchtop NMR</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/knauer_hplc_benchtop_nmr_web_638c60e13c.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Preparative HPLC is essential for isolating compounds and removing impurities in pharmaceutical and chemical industries. Non-destructive, fast analysis is preferred to maintain yield and enable at-line monitoring, avoiding lengthy sample preparation. Recent advancements in benchtop NMR spectrometers (up to 90 MHz) allow quick analysis without deuterated solvents. In this proof-of-concept study we purified a pharmaceutical sample via HPLC and confirmed its identity using benchtop NMR. Fractions (0.7–4.4 mM) were analyzed without preparation, using only 300 µL per sample, ensuring rapid batch control with no product loss. This study demonstrates the seamless integration of HPLC and benchtop NMR.</p><h4><strong>Experimental</strong></h4><h5><strong>Preparative HPLC Method</strong></h5><p>Eluent 20:80 Ethanol/water, isocratic mode, flow rate 12.5 ml/min, detection UV254 nm 2 Hz, 500 µl stacked injection, automated fractionation with threshold function, column temperature ambient, Column Eurospher II 100-10 C18 150x20 mm&nbsp;</p><h5><strong>Analytical HPLC Method</strong></h5><p>Eluent 20:80 Ethanol /water + 0.1% formic acid, isocratic mode, flow rate 1 ml/min, detection UV254 nm 10 Hz, 5 µl partial loop injection, column temperature 25 °C, Column <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1633"><strong>Eurospher II 100-5 C18</strong></a> 150x4 mm&nbsp;</p><h5><strong>NMR Method&nbsp;</strong></h5><p>NMR spectra were acquired on a <strong>Magritek Multi-X Ultra 80 MHz benchtop NMR system</strong> using an adapted WET sequence for solvent suppression. 32 scans were acquired with a repetition time of 15s. WET parameters were adjusted for each sample to yield optimal suppression for all solvent signals. Pooled HPLC fractions were analysed directly without addition of deuterated solvents or other sample preparation.</p><h4><strong>Conclusion</strong></h4><p>This proof-of-concept study successfully shows that HPLC and benchtop NMR can be used together easily. Samples can be analyzed non-destructively within minutes and without any further preparation due to highly efficient suppression of multiple solvent signals. Compounds can be identified without the need of reference standards. This study shows that NMR can provide a valid compatible alternative detector for HPLC fraction analysis.</p><h3><strong>2. Shimadzu: DIC Analysis in High-Speed Tensile Test of CFRP</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00854_en_fb6c68b858.pdf"><strong>Full PDF for download</strong></a></li></ul><h4><strong>User Benefits</strong></h4><ul><li>The <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=HPV-X3"><strong>HPV-X3</strong></a> is suitable for observation of fracture of brittle materials such as CFRP because it has a maximum recording speed (framerate) of 20 Mfps.&nbsp;</li><li>The HPV-X3 provides improved DIC analysis performance, as its resolution is 3 times higher than that of the conventional highspeed video camera.</li><li>The HITS-TX impact testing machine enables testing at a maximum speed of 20 m/s</li></ul><p>Carbon fiber reinforced plastic (CFRP) is a material with particularly high specific strength, even among composite materials, and used in various applications such as transport aircraft, taking advantage of its excellent mechanical properties. Because CFRP consists of carbon fibers and resin, it shows complex fracture behavior, and brittle fracture progresses from damage as the point of origin. Since fracture proceeds instantaneously, a high-speed video camera with a high recording speed is necessary when observing fracture of CFRP. One method for measuring strain in a high-speed tensile test is Digital Image Correlation (DIC), which visualizes the distribution of strain at the specimen surface by comparing a random pattern on the specimen surface before and after deformation. Thus, a high-speed video camera with a high resolution is also necessary in order to improve the DIC performance.&nbsp;</p><p>Newly-developed <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=HPV-X3"><strong>Hyper Vision HPV-X3 high-speed video camera</strong></a> is extremely well-suited to DIC analysis of CFRP, as its recording speed is 2 timesfaster and itsresolution is 3 times higher than the conventional HPV-X2. In this experiment, a high-speed tensile test of CFRP was conducted using a HITS-TX high-speed tensile testing machine. The condition of the test was observed with the new HPV-X3, and the strain distribution of the CFRP test piece was clarified by a DIC analysis of the recorded images.</p><h4><strong>Test Piece and Test Instruments</strong></h4><p>A CFRP quasi-isotropic laminate was used as the test piece. Aluminum foil was glued to the back side of the test piece, and the timing when the electrical conduction of the aluminum foil was interrupted by fracture of the test piece was used as the trigger for video recording. The HITS-TX was used in the highspeed tensile test. The HPV-X3 was used in fracture observation, and for comparison, the fracture process was also recorded with the conventional HPV-X2 camera. Fig. 1 shows the condition of the test, and Table 1 and Table 2 show the equipment composition and test conditions, respectively.</p><h4><strong>DIC Analysis Results in High-Speed Tensile Test</strong></h4><p>Fig. 3 shows the DIC analysis results in the high-speed tensile test. The color bar was set to display the color purple at around 0 % strain and red at around 2 % strain. Fig. 3 (1) is an image at the start of the test. After the test started, strain in the 45˚ direction concentrated from the lower left to the right central area, as can be seen in Fig. 3 (2). Then, as the test progressed, a condition of increasing strain in the initial area of strain concentration can be seen in Fig. 3 (3) to (9). A crack occurs in Fig. 3 (10), and strain decreases in the crack area. Following this, the test piece fractured completely in Fig. 3 (11) and (12), and a decrease in the strain in the area around the crack could be observed.</p><h4><strong>Conclusion</strong></h4><p>A high-speed tensile test of CFRP was conducted using a HITSTX high-speed tensile testing machine and the newlydeveloped HPV-X3 high-speed video camera, and the strain distribution at the time of test piece fracture was visualized by a DIC analysis. Because the HPV-X3 offers a 3 times higher resolution and a 2 times faster recording speed than the conventional device, it is a suitable high-speed video camera for fracture observation and DIC analysis in impact tests of brittle materials such as CFRP. The measurement system introduced in this Application News article is a useful tool for the development of composite materials.</p><h3><strong>3. Thermo Fisher Scientific: Polymer analysis using femtosecond-laser-ablation depth profiling</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/xps_depth_profiling_polymer_analysis_appnote_c731e2e7e0.pdf"><strong>Full PDF for download</strong></a></li></ul><p>X-ray photoelectron spectroscopy (XPS) is an essential polymer characterization technique that can identify not just the elemental composition but also the chemical state of polymer samples. This allows the chemical bonding environment to be deduced and, as a result, the polymer species can be identified. This can all be achieved with minimal sample preparation thanks to the built-in charge-compensation systems found in XPS instruments, which simplify the analysis of insulating samples.&nbsp;</p><p>The introduction of gas cluster ion sources (GCIS), such as the Thermo Scientific™ MAGCIS Dual Mode Ion Source, marked a major step forward in polymer analysis. Unlike monatomic ion beams, GCIS are able to clean surface contamination, and etch away layers of sample material, without damaging the underlying chemistry of organic samples such as polymers. As a result, GCIS has allowed chemical-damage-free analysis to reach micrometers below the sample surface. This depth of analysis can be highly time consuming, however, and total experimental times can range from multiple hours to days.&nbsp;</p><p>The addition of femtosecond-laser-ablation (fs-LA) technology is alleviating this, allowing for XPS spectra to be collected from a depth of tens of µm into a sample in a matter of minutes. This is because the laser ablation occurs on the femtosecond timescale (i.e., nearly instantaneously), effectively eliminating the material removal time of the depth profile. Additionally, unlike other material removal techniques such as atomic ion beam impact (where a collision cascade effect is induced to remove surface material) fs-LA is capable of removing material without chemical damage due to the ultrashort columbic explosion process used to eject the material. The resulting profile is therefore representative of the actual sample chemistry.</p><h4><strong>Paint layer analysis&nbsp;</strong></h4><p>In traditional ion-beam depth profiling, analytical depths are generally limited to several hundred nanometers, up to a micrometer. This means that it is extremely difficult to analyze the interfaces between multiple micrometer-thick layers in a sample without the use of additional techniques such as ultralow-angle microtome (ULAM) sectioning.¹ The introduction of fs-LA technology, however, allows micrometers of material to be removed rapidly, enabling the characterization of layered samples.&nbsp;</p><p>As an example, a multi-layer paint specimen (provided by the University of Surrey, UK) was depth profiled using the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2206"><strong>Thermo Scientific™ Hypulse™ Surface Analysis System</strong></a>. The sample consists of an aluminum substrate coated with a 5 µm layer of polyurethane primer followed by a 25 µm PMMA/PVdF topcoat.</p><p>The depth profile was generated using a 1,030 nm laser with an energy of 250 µJ for ablation, along with a 30 µm X-ray spot size for data collection. XPS spectra were collected after each ablation cycle to obtain an atomic concentration plot through both paint layers into the substrate material (Figure 2).&nbsp;</p><p>5 distinct regions throughout the analyzed depth can be seen. The top surface of the sample consists of an adventitious layer of carbon contamination, which is commonly found on samples exposed to air. The second layer is the 25 µm PVdF/PMMA topcoat, and the third layer is the PU primer. Between the primer and the final aluminum substrate layer, a clear increase in adventitious carbon is observed, similar to the signal seen at the top sample surface. This suggests residual carbon contamination was present on the substrate before the paint layers were applied.&nbsp;</p><p>It would take hours to produce the XPS depth profile shown in Figure 2 using traditional ion beam approaches. Even with the use of a monatomic beam with a high energy per atom, the length of the analysis could still be just as long, depending on the sample material, and would sacrifice the chemistry of any organic/polymeric materials. The combination of fs-LA depth profiling and snapshot XPS acquisition, meanwhile, can reduce the experimental time to less than 20 minutes without inducing any chemical damage to the sample surface.</p><h4><strong>Summary&nbsp;</strong></h4><p>One of the most critical aspects of successful polymer XPS depth profiling is the preservation of the sample chemistry during material removal. Additionally, experiment time can be a significant limiting factor, and thicker samples could lead to reduced time for repeat experiments. The femtosecond laser ablation capabilities of the Hypulse Surface Analysis System provide fast, efficient, and chemical-damage-free material removal from a sample surface, enabling analysis multiple micrometers into a sample. This means that hidden sample interfaces, that were largely inaccessible before, can now be analyzed easily and efficiently</p>

News from LabRulezICPMS Library - Week 19, 2026

Article

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Application

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Science and research

Software

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

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Wiley

LECO AC500 Isoperibol Calorimeter for Gross Calorific Content

The LECO AC500 is a compact isoperibol calorimeter for precise calorific value determination in solid and liquid fuels, featuring integrated circulation, multiple analysis modes, and high-temperature accuracy.

<p><strong>The AC500 determines calorific value—one of the most important indicators of quality and value in solid and liquid fuels—by accurately measuring the heat released during sample combustion. This standalone benchtop isoperibol calorimeter features a fully integrated circulation system, delivering a compact footprint without the need for external heaters or coolers.</strong></p><p>An integrated water-measuring station simplifies sample preparation, while a high-precision electronic thermometer with a resolution of 0.0001 °C records temperature every six seconds. Two-channel temperature correction is enabled through continuous monitoring of both the outer jacket and the calorimeter bucket, ensuring reliable and reproducible calorific value results across a wide range of operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_AC_500_Isoperibolicky_kalorimetr_pro_hrube_spalne_teplo_2_c9240dba59.jpg" alt="LECO AC500" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_AC_500_Isoperibolicky_kalorimetr_pro_hrube_spalne_teplo_2_c9240dba59.jpg 245w," sizes="100vw" width="1416" height="674"></p><h4><strong>Key Features</strong></h4><ul><li>High-precision calorific value determination across varying sample sizes and ambient conditions</li><li>Fully integrated circulation system for compact, standalone operation</li><li>Integral water-measuring station to simplify sample preparation</li><li>Electronic thermometer with 0.0001 °C resolution for precise temperature measurement</li><li>Temperature recorded every six seconds for accurate heat profile tracking</li><li>Two-channel temperature correction via constant monitoring of jacket and bucket</li><li><strong>Support for multiple analysis modes:</strong><ul><li>Regnault-Pfaundler</li><li>Precision</li><li>Predictive</li></ul></li><li>Combustion vessel options with fuse wire or string ignition</li><li>Optional string-ignition vessel eliminates wire connections for simplified handling</li></ul><h4><strong>Available Models</strong></h4><ul><li><strong>AC500</strong> – without combustion vessel</li><li><strong>AC500</strong> – with wire igniter vessel</li><li><strong>AC500</strong> – with string igniter vessel</li></ul>

LECO AC600 Semi-Automatic Isoperibol Calorimeter Gross Calorific Content

LECO AC600 is a semi-automatic isoperibol calorimeter delivering fast, accurate calorific value determination for solid and liquid fuels using TruSpeed™ technology and ergonomic vessel design.

<p><strong>The LECO AC600 semi-automatic isoperibol calorimeter is designed for accurate determination of calorific content in solid and liquid fuels. Combining semi-automated operation with LECO’s thermodynamic TruSpeed™ technology, the AC600 delivers rapid analysis times and high throughput without compromising accuracy or precision.</strong></p><p>A newly designed, lightweight, ergonomically optimized combustion vessel simplifies handling and significantly reduces operator strain. Together with intelligent software control and robust calorimeter design, the AC600 sets a benchmark for modern calorimetry laboratories focused on productivity, repeatability, and long-term reliability.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_AC_600_Poloautomaticky_isoperibolicky_kalorimetr_pro_hrube_spalne_teplo_2_8108ca8887.jpg" alt="LECO AC600" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_AC_600_Poloautomaticky_isoperibolicky_kalorimetr_pro_hrube_spalne_teplo_2_8108ca8887.jpg 188w," sizes="100vw" width="1455" height="1204"></p><h4><strong>Key Features</strong></h4><ul><li>Enhanced automation for increased laboratory productivity</li><li>Rapid analysis with results available in as little as 5 minutes</li><li>TruSpeed™ thermodynamic model enables fast measurements without loss of accuracy</li><li>Ergonomically designed, lightweight combustion vessel improves handling and heat transfer</li><li>Integrated vessel charging station simplifies oxygen filling</li><li>Quick-release depressurization ports speed up vessel handling between runs</li><li>Intuitive software with virtually unlimited data storage capacity</li><li>Simplified data management and compatibility with a wide range of LIMS platforms</li></ul><h4><strong>Theory of Operation</strong></h4><p>The LECO AC600 semi-automatic calorimeter determines the calorific value of organic materials such as coal, coke, and fuel oils by precisely measuring the heat released during combustion in a controlled environment.</p><p>A prepared sample is placed into a combustion vessel, which is then pressurized with oxygen. The vessel is automatically lowered into a water-filled bucket inside the instrument. The bucket is sealed using an inflatable bladder, while the surrounding jacket is also filled with water and maintained at a constant, controlled temperature characteristic of an isoperibol calorimeter system.</p><p>Following ignition of the sample, the temperature of both the bucket and jacket water is continuously measured using a high-resolution electrical thermometer with a resolution of 0.0001 °C. Temperature data are collected every second throughout the analysis.</p><p>The software allows correction of results for factors such as ignition wire contribution, nitrogen, sulfur, moisture, and ash content when required. Two data evaluation modes are available:</p><ul><li><strong>Delta T Mode</strong>, where the calorific value is calculated from the maximum temperature rise of the bucket</li><li><strong>TruSpeed™ Mode</strong>, a thermodynamic model developed by LECO that accounts for heat capacities of system components and energy transfer within the calorimeter</li></ul><p>The TruSpeed™ mode enables the AC600 to achieve rapid, 5-minute analysis cycles while maintaining excellent accuracy and precision.</p><h4><strong>Models</strong></h4><ul><li><strong>AC600</strong> – with standard combustion vessel</li><li><strong>AC600</strong> – with halogen-resistant combustion vessel</li><li><strong>Dual AC600</strong> – with standard combustion vessels</li><li><strong>Dual AC600</strong> – with halogen-resistant combustion vessels</li></ul>

LECO AF700 Coal and Coke Ash Fusibility Determinator

LECO AF700 is an automated ash fusion determinator for precise measurement of IT, ST, HT, and FT temperatures, featuring digital imaging, image recognition, enhanced safety, and high-durability furnace design.

<p><strong>The LECO AF700 Ash Fusion Determinator is designed to increase laboratory throughput by automatically determining ash fusibility temperatures, including Initial Deformation (IT), Softening (ST), Hemispherical (HT), and Flow (FT) temperatures. Built with enhanced robustness, integrated safety features, and advanced digital imaging, the AF700 delivers reliable, repeatable ash fusion analysis with minimal operator intervention.</strong></p><p>The system combines precise furnace temperature control, high-resolution digital imaging, and intelligent software-driven image recognition to support objective and defensible ash fusibility determinations. Digital archiving capabilities ensure full traceability and easy review of results, supporting both routine testing and confirmatory analysis.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_AF_700_Analyzator_tavitelnosti_popela_66f55ca93d.jpg" alt="LECO AF700" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_AF_700_Analyzator_tavitelnosti_popela_66f55ca93d.jpg 166w," sizes="100vw" width="1030" height="970"></p><h4><strong>Key Features</strong></h4><ul><li><strong>Integrated high-stability imaging system</strong><ul><li>Permanently mounted camera isolated from the high-temperature furnace zone</li><li>Integrated viewing system ensures consistent alignment of mirrors, camera, and furnace</li></ul></li><li><strong>Automated, objective ash fusion determination</strong><ul><li>Image Recognition Functions (IRF) automatically identify IT, ST, HT, and FT</li><li>Optional automatic analysis termination once all deformation points are reached</li></ul></li><li><strong>Complete digital documentation</strong><ul><li>Full image history archived for every analysis</li><li>Archived images can be retrieved for review, confirmation, or subjective evaluation</li><li>Supports storage on DVD, CD-RW, or hard drive</li></ul></li><li><strong>Enhanced laboratory safety</strong><ul><li>Door interlocks prevent unsafe furnace operation</li><li>Integrated carbon monoxide detector improves operator protection</li></ul></li><li><strong>Improved low-temperature visibility</strong><ul><li>LED light ring enables clear observation of early deformation points</li></ul></li><li><strong>High-durability furnace design</strong><ul><li>Six-element, high-endurance heating furnace ensures long service life</li><li>Designed for sustained operation under demanding laboratory conditions</li></ul></li></ul><h4><strong>Theory of Operation</strong></h4><p>The LECO AF700 automatically monitors ash cone deformation temperatures in coal ash and coke ash samples. Prepared ash cones are positioned on a ceramic tray and inserted into a programmable, high-temperature furnace.</p><p>The operator selects an analytical method defining the furnace atmosphere—oxidizing or reducing—as well as the temperature ramp rate in degrees Celsius per minute. Once the furnace reaches the method-defined starting temperature, a digital camera begins collecting images at defined intervals.</p><p>Image Recognition Functions (IRF) within the software analyze sample geometry in real time to automatically identify standard ash fusibility temperatures: Initial Deformation (IT), Softening (ST), Hemispherical (HT), and Flow (FT). The system can optionally terminate the analysis automatically once all deformation points have been detected for all samples, improving throughput while extending furnace lifetime.</p><p>All images collected during analysis are digitally archived, allowing easy retrieval for verification, reporting, or subjective reassessment of deformation temperatures when required.</p><h4><strong>Models</strong></h4><ul><li><strong>AF700</strong> – Single Furnace System</li><li><strong>AF700</strong> – Dual Furnace System</li></ul>

LECO TGA801 Thermogravimetric Analysis of Moisture, Ash, Volatile Content, and Loss-on-Ignition

LECO TGA801 is a macro thermogravimetric analyzer for automated determination of moisture, ash, volatiles, and LOI with high precision, flexible methods, and up to 19-sample capacity.

<p><strong>The LECO TGA801 builds on the proven performance of previous generations of LECO macro TGA instruments, delivering enhanced robustness, precision, and operational reliability. Combining state-of-the-art hardware with an intuitive touch-screen software platform and rugged construction, the TGA801 enables accurate, high-precision thermogravimetric constituent analysis.</strong></p><p>The instrument supports determination of moisture, ash, volatile content, and Loss-on-Ignition (LOI) in a wide range of organic, inorganic, and synthetic materials. Designed in accordance with <strong>ASTM standardized methods</strong>, the TGA801 is well suited for laboratories serving industries such as coal, cement, catalysts, food, and feed analysis.</p><p>Macro thermogravimetric analysis using nominal 1 g sample masses replaces traditional, labor-intensive gravimetric workflows that rely on multiple manual weighing steps, ovens, muffle furnaces, and desiccators. Flexible method configuration, automation, and advanced hardware allow the TGA801 to deliver a fully automated analysis sequence, requiring only the initial manual sample weighing to maximize laboratory productivity.</p><p><strong>Cornerstone Mobile remote software</strong> extends instrument visibility beyond the laboratory, enabling users to monitor batch progress, instrument status, and performance from a smartphone, tablet, or PC.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/TGA_801_Termogravimetricka_analyza_vlhkosti_popela_tekavych_latek_a_ztraty_zihanim_4_46d807b3c3.jpg" alt="LECO TGA801" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_TGA_801_Termogravimetricka_analyza_vlhkosti_popela_tekavych_latek_a_ztraty_zihanim_4_46d807b3c3.jpg 245w," sizes="100vw" width="757" height="476"></p><h4><strong>Key Features and Benefits</strong></h4><ul><li><strong>High-throughput automated thermogravimetric analysis</strong><ul><li>Simultaneous analysis of up to 19 samples</li></ul></li><li><strong>Intelligent endpoint determination</strong><ul><li>Automatic endpoint recognition based on sample mass constancy</li></ul></li><li><strong>High-precision mass measurement</strong><ul><li>Integrated, thermally isolated balance with 0.0001 g resolution</li><li>Low drift design ensures stable and repeatable results throughout the analysis cycle</li></ul></li><li><strong>Accurate and controlled furnace operation</strong><ul><li>Precise temperature ramping and set-point control</li><li>Minimal temperature overshoot for excellent thermal accuracy</li></ul></li><li><strong>Uniform sample exposure</strong><ul><li>Rotating automation sequence ensures consistent furnace conditions for all samples</li></ul></li><li><strong>Flexible atmosphere control</strong><ul><li>Automated control of furnace atmosphere and gas flow</li><li>Supports air, nitrogen, or oxygen environments</li></ul></li><li><strong>Method versatility</strong><ul><li>Configurable method steps allow emulation of classic gravimetric test methods</li></ul></li><li><strong>Enhanced safety and reliability</strong><ul><li>Furnace lid remains locked open during power or pneumatic interruptions</li></ul></li></ul><h4><strong>Applications</strong></h4><p>The TGA801 is ideal for thermogravimetric analysis across a wide range of materials, including:</p><ul><li>Coal and coke</li><li>Combustion residues</li><li>Biomass materials</li><li>Catalyst materials</li><li>Soils and ores</li><li>Ceramics</li><li>Gypsum and building materials</li><li>Hydrated lime and cement</li><li>Flour and milled products</li><li>Feed materials</li><li>Resins and polymers</li></ul><h4><strong>Models</strong></h4><ul><li><strong>TGA801</strong> – Thermogravimetric Analyzer</li><li><strong>TGA801 Dual Furnace Package</strong> – Dual furnace configuration for increased throughput and flexibility</li></ul><h4><strong>Theory of Operation</strong></h4><p>The TGA801 determines weight loss associated with moisture, volatiles, ash, and Loss-on-Ignition by measuring sample mass as a function of temperature under precisely controlled atmospheric conditions. The multi-sample furnace design enables simultaneous analysis of up to 19 samples.</p><p>An analysis begins after samples are logged and empty crucibles are placed into the sample carousel. The system automatically records the mass of each empty crucible before prompting the operator to load samples. Once loaded, initial sample weights are recorded and the selected furnace temperature program is initiated.</p><p>The furnace atmosphere is controlled using one of three analytical gases—air, nitrogen, or oxygen—depending on the analytical step. Each method step can be configured with specific temperature set points, ramp rates, atmospheres, and durations defined either by fixed time or mass constancy. Preset method steps for moisture, volatile matter, and ash determination are available and commonly used.</p><p>Throughout the analysis, crucibles are automatically indexed and lowered onto the balance pedestal to record sample mass. Mass changes are tracked sequentially for each sample, and results are typically reported as percent mass loss at the end of each step. Additional or alternative calculations can be defined within the method as needed.</p><p>An external PC running LECO Cornerstone® software manages all quantitative calculations, stores analytical data, and provides comprehensive reporting and traceability.</p>

LECO TGM800 Thermogravimetric Moisture Determinator

LECO TGM800 is an automated thermogravimetric moisture analyzer enabling simultaneous analysis of up to 16 samples with intelligent endpoint detection and integrated Cornerstone® software.

<p><strong>The LECO TGM800 delivers a high-precision, fully automated solution for moisture determination by replacing traditional, time-consuming loss-on-drying methods with a modern thermogravimetric approach. Designed to improve laboratory efficiency and consistency, the TGM800 combines multi-sample automation, intelligent endpoint detection, and intuitive Cornerstone® touch-screen software.</strong></p><p>Capable of analyzing up to 16 samples simultaneously, the TGM800 significantly reduces hands-on time while ensuring accurate and reproducible moisture results. Its versatility makes it suitable for a wide range of applications, including food, feed, milled products, and agricultural materials.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_TGM_800_Termogravimetricky_analyzator_vlhkosti_11_6e4d62debb.jpg" alt="LECO TGM800" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_TGM_800_Termogravimetricky_analyzator_vlhkosti_11_6e4d62debb.jpg 245w," sizes="100vw" width="1309" height="805"></p><h4><strong>Key Features and Benefits</strong></h4><ul><li><strong>Automated loss-on-drying moisture determination</strong><ul><li>Up to 16 samples using 1.5-inch aluminum foil crucibles</li><li>Up to 10 samples using 2.4-inch aluminum foil crucibles</li></ul></li><li><strong>Increased laboratory throughput</strong><ul><li>Rapid oven temperature ramping</li><li>Fast cooling following analysis completion</li></ul></li><li><strong>Intelligent drying control</strong><ul><li>Automatic endpoint recognition based on sample mass constancy</li><li>Optimized drying times without unnecessary over-drying</li></ul></li><li><strong>Reduced operator workload</strong><ul><li>Minimal sample interaction requirements</li><li>Automated weighing and mass tracking throughout the analysis</li></ul></li><li><strong>Integrated, space-saving design</strong><ul><li>Built-in 10.1-inch color LCD touch-screen</li><li>Cornerstone® software integrated directly into the instrument for efficient, ergonomic operation</li></ul></li></ul><h4><strong>Applications</strong></h4><p>The TGM800 is suitable for moisture determination across a broad range of sample types, including:</p><ul><li>Food and food ingredients</li><li>Feeds and pet food</li><li>Milled products</li><li>Agricultural and plant-based materials</li></ul><h4><strong>Theory of Operation</strong></h4><p>The TGM800 is a thermogravimetric analyzer designed to directly measure moisture content using a controlled loss-on-drying technique. Moisture is determined by continuously measuring sample mass as a function of oven temperature while precisely controlling atmosphere and ventilation conditions.</p><p>The instrument integrates a computer, a four-place analytical balance, and a multi-sample oven that enables the simultaneous analysis of up to 16 samples. Once an analysis method is selected, empty aluminum foil crucibles are loaded into the oven carousel. The system automatically tares each crucible before sequentially presenting them to the operator for sample loading. Initial sample masses are measured and stored automatically.</p><p>After sample loading is complete, the analysis begins with controlled oven temperature ramping and the initiation of ventilation. Each crucible is automatically indexed and lowered onto the balance pedestal, where the sample mass is recorded. This process continues sequentially for all samples throughout the analysis.</p><p>Mass loss is continuously monitored for each sample while the oven temperature and ventilation rate are regulated according to the selected method. Analysis duration can be defined either by a fixed time or by achieving sample mass constancy. Moisture content is calculated as percent mass loss and reported individually for each sample at the conclusion of the run.</p><p>The intuitive touch-screen interface provides full access to method creation, analysis control, diagnostics, and reporting in a highly organized environment. Methods can be customized with user-defined oven temperatures, ramp rates, atmospheres, and ventilation settings. Real-time plots of sample mass loss and temperature are displayed on screen, while all results and calculations are automatically stored and managed by the software.</p><p>By enabling batch processing of up to 16 samples with automated mass measurements, the TGM800 maximizes laboratory productivity, analytical consistency, and overall workflow efficiency.</p>

Products focused on Thermal Analysis distributed by LECO Instrumente Plzeň - page 1

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