<div>In 2025, two outstanding young female researchers, Drs. Filipa Oliveira and Stella Gonsales, both originally from outside the Czech Republic, received prestigious JUNIOR STAR grants from the Czech Science Foundation (GAČR). JUNIOR STAR enables early career researchers to establish their own independent research groups and significantly advance their scientific careers. Their projects reflect cutting-edge research in materials science and sustainable chemistry, highlighting the international reach and welcoming research environment at UCT Prague.Dr. Stella Gonsales is also a&nbsp;laureate of the Dagmar Procházková Fund, which supports promising scientific projects in sustainable and advanced organic chemistry. Another recipient of this grant is Dr.&nbsp;Anna Valunchene, whose research complements UCT Prague’s&nbsp;sustainability goals. This dual funding underlines Stella’s&nbsp;remarkable achievements and the wide scope of innovative research at UCT Prague.Both Filipa and Stella are actively looking for motivated students and researchers to join their teams, offering a&nbsp;unique opportunity to work at the forefront of science in a&nbsp;vibrant and supportive environment.</div><div><h3>Interview with Filipa Oliveira: Pioneering tubular MXenes for supercapacitor energy storage</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Filipa Oliveira" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg 235w," sizes="100vw" width="1500" height="999"><h5>Congratulations on receiving the JUNIOR STAR grant. What does this achievement mean for you and how will it influence your next steps?</h5>Thank you. It’s&nbsp;a huge achievement to receive this prestigious grant. It gives me the opportunity to establish my own research group here at UCT Prague. The JUNIOR STAR program supports talented young scientists in building their teams and accessing resources. This means I&nbsp;now have the support to focus fully on my research about tubular MXenes and their application in supercapacitors.<h5>Why are tubular MXenes so innovative for energy storage?</h5>MXenes are a family of 2D materials with a layered, accordion-like structure and known for their excellent electrical conductivity. Our innovation is to produce them with a tubular morphology, combining the high conductivity and redox activity in superior ion-transport tubular architectures. Their open, hollow structure increases surface area, ion diffusion and charge transport. This could translate to faster, more efficient supercapacitors, critical for sustainable energy technologies.<h5>How did your scientific interest lead you to this field?</h5>Global challenges around green energy made me focus on materials that can improve energy storage. MXenes stood out because changes in the composition and structure can significantly change their properties. I&nbsp;was curious about how tuning different metallic elements in a&nbsp;tubular morphology can impact how MXenes perform and how to optimize them for practical applications.<h5>What challenges have you encountered?</h5>One major challenge is their stability: MXenes oxidize quickly which deteriorates performance. Another challenge is understanding how synthesis conditions of both MXenes and their precursors (MAX phases) must be adapted to create and preserve tubular morphologies since their synthesis mechanisms differ from conventional MXenes. Additionally, effectively communicating the project in the funding proposal to show to reviewers why I&nbsp;should obtain this funding was also a&nbsp;challenge, but thanks to mentorship and collaboration, I&nbsp;overcame these hurdles.<h5>What motivates you most about working at UCT Prague?</h5>The support from colleagues and the excellent technical infrastructure make it a&nbsp;great place to work. Also, being a&nbsp;former member of Professor Sofer’s&nbsp;leading group in 2D&nbsp;materials is inspiring and led me to this achievement.<h5>How do&nbsp;you see your professional development going in the future?</h5>The grant will help me build my own team, acquire equipment, and develop collaborations. My long-term vision is to contribute significantly to sustainable energy solutions and scientific progress both locally and internationally.<h5>You are looking for new team members. What kind of students or scientists do&nbsp;you need?</h5>We are currently welcoming enthusiastic master's&nbsp;students, PhD candidates, and postdocs who are passionate about materials science and eager to explore new frontiers in MXenes and energy storage. If you love curiosity-driven research and want to be part of a&nbsp;growing international team, we encourage you to join us.<h3>Interview with Stella Gonsales: Catalysis as a&nbsp;key to polymer recycling</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Stella Gonsales" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg 233w," sizes="100vw" width="1499" height="1000"><h5>Congratulations on your JUNIOR STAR grant and also being awarded the Dagmar Procházková Fund. What does receiving both mean to you and how will they shape your work?</h5>Thank you. Both grants are hugely important. The JUNIOR STAR grant allows me to expand my group and resources for research into catalytic recycling of polymers, while the Dagmar Procházková Fund supports complementary projects such as synthesizing new biodegradable polymers. Together, they help tackle the plastic waste problem from multiple scientific angles.<h5>Could you explain your research in layperson’s&nbsp;terms?</h5>Most plastics have strong carbon-carbon bonds. We insert more cleavable double bonds into polymers that can be broken down by catalytic reactions called olefin metathesis, making recycling on a&nbsp;molecular level more feasible.<h5>What makes the JUNIOR STAR grant particularly important for your research and career development?</h5>The JUNIOR STAR grant is a&nbsp;prestigious and highly competitive funding opportunity aimed at excellent early-career scientists within eight years of completing a&nbsp;Ph.D. It provides up to 25&nbsp;million CZK over five years, allowing recipients to establish independent research groups and pursue innovative scientific topics. This grant is crucial for building a&nbsp;solid foundation, acquiring necessary equipment, and recruiting talented team members, enabling the pursuit of ambitious projects that can significantly contribute to both the Czech scientific community and global challenges.<h5>What collaborations support your research?</h5>The collaboration between UCT Prague and the Institute of Organic Chemistry and Biochemistry (IOCB) of the Czech Academy of Sciences primarily provides financial and technical support. The cooperation helps with funding, access to advanced equipment, and operational resources needed for running research projects.<h5>What advice would you give young scientists aiming to succeed?</h5>I&nbsp;encourage thinking outside the box and combining diverse knowledge you acquire during your career to address complex problems that are exciting and relevant to you.<h5>Your laboratory bears the name of Otto Wichterle and was ceremonially opened in the presence of Professor Kamil Wichterle. How does Wichterle’s&nbsp;legacy inspire you personally?</h5>Wichterle’<s>’</s>s&nbsp;innovative spirit motivates me to push creative boundaries and pursue novel solutions in sustainable chemistry.<h5>What are your visions for the future?</h5>I&nbsp;aim to build a&nbsp;research group that advances sustainable polymer recycling, contributing to environmental responsibility and the circular economy.<h5>You are recruiting people for your team. What skills do&nbsp;they need to have?</h5>We are looking for talented Ph.D. students and postdocs experienced in air-sensitive catalysis techniques. If you want to contribute to transformative research in sustainable chemistry and join a&nbsp;dynamic team, this is a&nbsp;great opportunity.</div>

JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science

The clean laboratories of the CEITEC Nano research infrastructure at CEITEC Brno University of Technology house a four-meter-tall TITAN transmission electron microscope for researching materials at the atomic level. This scientific device underwent two significant upgrades this year, entailing the addition of two state-of-the-art technologies: an electromagnetic lens aberration corrector for scanning transmission electron microscopy and an electron energy loss spectrometer equipped with cameras featuring today's most sensitive sensors. Thanks to these upgrades, TITAN has become a state-of-the-art device that can only be found in a few of the world's most advanced laboratories in this configuration.The TITAN Themis microscope was manufactured in 2015 by FEI (now Thermo Fisher Scientific) and has been in use at CEITEC BUT since 2016. At the time of its acquisition, it was one of the most powerful commercially available electron microscopes; however, it lacked a corrector for optical defects in the lenses that form the narrow electron beam scanning the sample. This meant limited resolution and a lower ability to analyze the chemical composition and physical properties of materials at the atomic level."The latest generation S-CORR aberration corrector allows the electron beam to be focused to a size up to twice smaller than an atom, while also providing a higher electron current. This has led to a significant increase in the resolution and analytical capabilities of the microscope," explains Jan Michalička, guarantor of the TITAN microscope. "Thanks to the new corrector, our microscope achieves the world's highest standard in resolution, specifically less than 50 picometers at an acceleration voltage of 300 keV." For better understanding, he adds a comparison: "Imagine the ratio of the size of a golf ball to that of planet Earth. That is exactly the ratio of an atom measuring 100 pm to a golf ball." Thanks to this resolution, scientists are not only able to image individual atoms, but also to determine their chemical nature through further analysis. This is of fundamental importance, for example, for the development of new catalysts for a more environmentally friendly chemical industry.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Differences in resolution before and after the installation of the aberration corrector." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg 245w," sizes="100vw" width="1600" height="904">The new corrector also allows the microscope to achieve high resolution even with low-energy electrons. This is crucial for studying highly sensitive materials such as graphene and other two-dimensional structures, which would be damaged by a stronger electron beam. "Electrons interact strongly with the sample and can damage it at the atomic level, for example, by changing its structure and chemical composition. With the new corrector, we can achieve atomic resolution even at the lowest acceleration voltage of 60 keV, allowing us to study even very sensitive materials in a virtually unchanged state," says Jan Michalička.<h4>The additional installation of a corrector at the CEITEC laboratory is unique worldwide</h4>Correctors of optical defects in electromagnetic lenses are an exceptional technology that has completely changed the world of electron microscopy. For a long time, they were considered technically impossible, and the absence of correctors essentially prevented research into the structure and properties of materials at the atomic level. It was not until the 1990s that a group of four scientists – Harald Rose, Max Haider, Knut Urban, and Ondřej Křivánek – succeeded in implementing this technology, for which they received the prestigious Kavli Prize in 2020. “Correctors basically consist of a pair of complex lenses, each containing a series of magnetic multipoles. In the case of the correctors invented by the first three scientists mentioned above, defects are removed by two hexapole lenses. Our new corrector is based on the same concept,” adds Jan Michalička.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Delivered aberration corrector prior to installation, with the TITAN microscope in the background." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg 117w," sizes="100vw" width="1600" height="2133">The additional installation of a corrector is unique in itself nowadays. Similarly complex technologies are usually installed during the manufacture of microscopes in the factory, in this case in the Netherlands. However, in Brno, the microscope was successfully upgraded with a corrector under the limited conditions of the laboratory. The installation of the corrector took about four months and required the dismantling of a large part of the microscope. "With this installation, we basically went back to the time when the four scientists mentioned above placed their prototype corrector on an older microscope, thus improving its performance," says Jan Michalička with a smile, adding that the most difficult part was the precise adjustment of the corrector and the entire optics of the microscope in order to achieve top parameters. "Even though most things are now controlled by computers, there are only a few people in the world who can install and tune a corrector to the highest level of accuracy. We are therefore extremely pleased to have had such experts at our disposal here."<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the aberration corrector – removal of the condenser." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg 117w," sizes="100vw" width="1600" height="2133"><h4>New spectrometer will enable scientists to see more</h4>The second and equally important upgrade to the TITAN microscope is the installation of the latest generation GIF Continuum HR electron energy loss spectrometer by Gatan. This is an approximately two-meter-long device installed horizontally under the microscope, whose main component is a magnetic prism that separates the electron beam according to their energy losses. The spectra of these losses provide enormous information about the sample, principally at the atomic level, as they are characteristic of the given state of the atoms, such as the valence arrangement of their electron shell. In combination with the new corrector, the spectra can be used to analyze the chemical bonds, arrangement in the crystal lattice, or the entire range of physical properties of atomic nanostructures down to the level of individual atoms.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the spectrometer beneath the microscope, magnetic prism." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg 117w," sizes="100vw" width="1600" height="2133">An essential part of the spectrometer are the state-of-the-art K3 and Stela cameras, which can directly detect and count individual electrons. At the same time, these cameras are extremely fast and their sensors capture data without noise. "These features of our cameras are key to measuring sensitive or dynamically changing samples and allow us to obtain information that was previously invisible to electron microscopes," says Jan Michalička.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installed high-performance spectrometer cameras." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg 117w," sizes="100vw" width="1600" height="2133">Last but not least, the spectrometer will open up completely new possibilities for measuring the magnetic properties of atomic structures. The new spectrometer will also be used for such experiments within the project TERAFIT, which financed this upgrade, and which focuses on research into materials for ultra-high capacity, speed, and energy efficiency in information technology. "The device is fully prepared for advanced experiments and the use of modern software tools, including artificial intelligence," adds Vojtěch Uhlíř, head of the Nanomagnetism and Spintronics research group at CEITEC BUT and one of the TERAFIT project investigators, highlighting the advantages of the spectrometer.Together with the corrector, the spectrometer represents a breakthrough in the analysis of material properties, and according to Jan Michalička, CEITEC now has one of the most powerful microscopes available. “In modern materials science, for example, in the development of magnetic memories, quantum materials, or 2D structures, it is necessary to see not only atoms, but also their behaviour in real time, including magnetic states. And it is this spectrometer that pushes the boundaries of what we can see and analyze.” Michal Urbánek, head of the CEITEC Nano laboratories and coordinator of the large research infrastructure <a target="_blank" rel="noopener noreferrer" href="https://www.czechnanolab.cz/">CzechNanoLab</a>, agrees with him, adding that the device can now contribute to scientific knowledge on a global scale. “Thanks to its integration into the large CzechNanoLab research infrastructure, this state-of-the-art microscope is accessible not only to scientists from CEITEC, but also to other institutions in the Czech Republic and abroad.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Final configuration of the TITAN microscope after installation of the aberration corrector and spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg 117w," sizes="100vw" width="1600" height="2133"><blockquote>Do you want to know more? Read the <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/upgrade-of-titan-transmission-electron-microscope-ranks-ceitec-among-elite-laboratories-expert-version/t11696?secret=40ab0267-ec7e-11f0-8f5d-2ed9ab825d4b">expert version</a> of this article.</blockquote>

Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories

Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Thermo Scientific Qtegra Intelligent Scientific Data Solution Software

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The Thermo Scientific™ Qtegra™ Intelligent Scientific Data Solution (ISDS) Software is a software platform built on four pillars, designed to deliver a complete solution for laboratories depending on productivity, scalability, and quality results to achieve their goals.<h5>Intelligent</h5>Built-in intelligence facilitates the full workflow from sample to results. Laboratories can be more reactive and profitable, with software that facilitates data driven decisions more efficiently.<h5>Scientific</h5>Developed with scientific integrity, Qtegra ISDS Software delivers high quality, reliable data that labs can trust. Data transparency lets laboratory personnel focus on other tasks while ensuring the utmost confidence in scientific collaboration.<h5>Data</h5>Data is the key to success for all laboratories. Qtegra ISDS Software can help labs excel at efficiently delivering consistent and relevant results with extensive tools to effectively acquire and manage data.<h5>Solution</h5>Laboratories can enjoy the familiarity of common software across multiple analytical systems while differentiating their offering through customizable and application specific workflows.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_2_78dbb0f7f5.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Qtegra Intelligent Scientific Data Solution Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_2_78dbb0f7f5.jpg 217w," sizes="100vw" width="1535" height="1101"><h4>Routine analysis</h4>High-throughput production and contract testing laboratories benefit from the assistance and economy imparted through Qtegra ISDS Software. The design facilitates integration in a growing lab ecosystem, while intuitive workflow features, interference management and pertinent data visualization lets laboratories acquire and interpret data confidently, quickly, and with ease. Laboratories performing routine testing can ensure profitability through productivity, quality data, and right first-time analysis.<h4>Scientific research</h4>Researchers looking to push the boundaries of innovation in their field can focus intently on science, confident in the knowledge that Qtegra ISDS Software provides flexibility of customized configurations with multiple acquisition modes and data processing tools to probe and access new data. Within a framework that is exceptionally easy to set up and switch between configurations, Qtegra ISDS Software enables research labs with multiple users to focus on the data rather than the analysis.<h4>Regulated industries</h4>Food, environmental and pharmaceutical QA/QC are amongst the sectors that are constantly being challenged to meet rising standards of quality and to comply with rigorous regulatory requirements, whether they be analytical or procedural. Qtegra ISDS Software recognizes these challenges and includes the necessary tools for laboratories to rapidly meet their compliance goals.<h4>Integrated lab assistant saves time, simplifies sample preparation, and intelligently assists data analysis.</h4><ul><li>Lab is quickly alerted to QC anomalies and samples above Maximum Allowable Concentrations.</li><li>Integrated, flexible QA/QC protocols minimize failures and prevent costly re-runs.</li><li>Intuitive color-coding of data and reports aids faster interpretation and decision making.</li><li>Calculations are transparently applied in real time and can be audited easily.</li><li>Prescriptive sample dilutions save labor and mitigate errors.</li><li>Intelligent uptake and rinses optimize throughput and limit carryover challenges.</li><li>Intelligent over-range autodilution of high concentration samples automatically generates usable data without user input or delays</li></ul><h4>It’s not just about finding answers, it’s about finding the right answers</h4>Qtegra ISDS Software helps labs achieve consistently accurate results through Interference Management. Many analytical techniques are prone to interferences that are difficult to remove. Qtegra ISDS Software can help identify and automatically mitigate interferences with full visibility.The Reaction Finder method development assistant for Thermo Scientific™ TQ-ICP-MS analyzers intelligently selects the optimal measurement mode settings based on method analytes, removing the complexity from TQ technology and dramatically decreasing method development time.Interelement correction (IEC) for the Thermo Scientific™ iCAP™ PRO Series ICP-OES provides the ability to correct for interferences based on intensity or concentration and conform with regulatory methods, such as EPA Method 200.7.<h4>Gain deeper insights and accelerate innovation with the flexibility of different data evaluation modes and specific workflows.</h4><h4>IRMS Chromatogram</h4>Enjoy simple and reliable compound-specific IRMS or trace elemental speciation with a fully integrated workflow including chromatographic device control and dedicated chromatographic data processing.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_IRMS_Chromatogram_7b0a3b5376.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Qtegra Intelligent Scientific Data Solution Software - IRMS Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_IRMS_Chromatogram_7b0a3b5376.jpg 245w," sizes="100vw" width="615" height="331"><h4>Laser ablation</h4>Expand capabilities in spatial and depth characterization with solutions to integrate laser ablation systems and split-flow analysis with third-party plug-ins and laser ablation specific data evaluation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_Laser_ablation_63e97c2a0a.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Qtegra Intelligent Scientific Data Solution Software - Laser ablation" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_Laser_ablation_63e97c2a0a.jpg 245w," sizes="100vw" width="613" height="338"><h4>Single particle</h4>Explore the expanding fields of single cell and nanoparticle analysis with automated workflows and dedicated sample handling packages.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_Single_particle_72be4bfa52.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Qtegra Intelligent Scientific Data Solution Software - Single particle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Thermo_Scientific_Qtegra_Intelligent_Scientific_Data_Solution_Software_Single_particle_72be4bfa52.jpg 245w," sizes="100vw" width="615" height="329"><h4>How do you manage large volumes of data in your laboratory?</h4>Leverage data management and flexible and intuitive data processing tools within Qtegra ISDS Software to excel at delivering consistent data on time.<h5>Data security</h5>Be confident with the knowledge that all data is secure and encrypted, yet easily accessible. The Qtegra ISDS Software LabBook concept ensures the whole analytical workflow from samples to results is stored in one secure container.<h5>LIMS integration</h5>Complete analysis descriptions can be directly imported into Qtegra ISDS Software, eliminating additional user input. Full results, both analytical and metadata, are immediately available after analysis for rapid turnaround of results.<h5>Data visualization</h5>Access relevant data quickly, with flexibility to dig deeper when needed, enabling quicker decisions and efficiency.<h5>Enhanced LabBook query</h5>Combine data across LabBooks for method validation, long-term performance, or finding relationships and gaining deeper insights into data.<h5>Flexible data export</h5>Export data in the format you need for archiving, reporting, or further data processing and interrogation.<h5>Comprehensive data reporting</h5>Automated report generation makes data management effortless. Customize report formats to fit with the needs of your customers.<h5>Data management</h5>Store and manage data effortlessly with an intuitive file browser.<h5>User management</h5>Control access for up to 5 user levels with comprehensive user management.

Thermo Scientific™ OXSAS™ Optical Emission Analytical Software

Thermo Scientific™ OXSAS™ software for OES enables fast, accurate metals analysis, method and calibration development, automation, and easy batch processing.

Perform rapid high quality metals analyses with a customizable software solution for optical emission spectrometers. Flexible and powerful Thermo Scientific™ OXSAS™ Optical Emission Analytical Software runs a variety of analyses, including the development of new analytical methods and calibrations as well as rapid high-quality routine elemental analysis.&nbsp;From simple tasks to complex jobs, the OXSAS software platform can perform many operations in a single click and is designed to evolve to meet your needs throughout your instrument's lifetime.<h3>📋 Template-Driven Analysis</h3><ul><li>Simple, comprehensive analysis of unknown samples.</li><li>Required analytical parameters and results processing details pre-defined in the routine analysis shortcut.</li><li>One click creation of a batch with all samples requiring analysis.</li><li>Automatic Method Choice selects the best analytical parameters set without user intervention.</li></ul><h3>⚙️ Fast Calibration</h3><ul><li>Multiple analyte curve display, instant base curve calculation, templates for editing and measuring of calibration standards.</li><li>Calibration curve determination using multi-variable regression with additive and multiplicative correction models.</li></ul><h3>🖥️ Familiar User Interface</h3><ul><li>Comprehensive post-treatment of results, including export and Microsoft™ Excel support.</li><li>Integrated Microsoft SQL Server Express relational database stores setup and analyses data.</li><li>Export and import facilities allow calibration standards, methods or grades to be easily transferred between instruments.</li><li>Full network and serial transmission capabilities.</li><li>Common software platform with the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products?manufacturerItems=3&amp;search=WDXRF">WDXRF Thermo Scientific series</a> instrument range.</li><li>Supports Thermo Scientific SMS system for full automation of sample analysis.</li><li>Monitoring and maintenance tools to maximize uptime.</li><li>Measurement Uncertainty and Conformance Probability on unknown samples analysis.</li><li>SCT Manager: status and history overview of instrument and method analytical capability.</li><li>Comprehensive, on-line contextual Help.</li><li>Real, on-line integrated SPC.</li><li>Key to Metals: comprehensive steel and nonferrous metals database with cross-reference.</li></ul><h3>🔌Instrument Compatibility</h3>Supports the Spark option of the:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1977">Thermo Scientific™ ARL iSpark™ Plus</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1213">ARL iSpark™</a></li><li>Thermo Scientific™ ARL 4460™ Optical Emission Spectrometer</li></ul>Allows complex calculations such as:<ul><li>element soluble/insoluble</li><li>element inclusions (number, size and chemical composition)</li><li>with no additional analysis time</li></ul><h3>🧩 Options for OXSAS Software</h3><ul><li>Charge correction: calculates material weights to correct out-of-spec charges.</li><li>SPC-Full: full graphical extension for on-line Statistical Process Control.</li><li>ARLcom: result transmission over network or serial via TCP/IP or to files/printers.</li><li>Remote Sample Definition: sample registration from external applications anywhere on the network.</li></ul><h3>✅ Compatible with:</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1977">ARL iSpark Plus Optical Emission Spectrometer</a></li><li>ARL iSpark Plus Fire Assay Analyzer</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1213">ARL iSpark Optical Emission Spectrometer</a></li><li>ARL 4460 Optical Emission Spectrometer</li><li>Thermo Scientific™ ARL™ Fire Assay Analyzer</li></ul>

Thermo Scientific™ OXSAS™ X-Ray Fluorescence Analysis Software

Thermo Scientific™ OXSAS™ is a powerful and customizable XRF software that enables fast, template-driven analysis, automation, and standardless quantification options.

Enhance your lab efficiency and productivity with a customizable software solution that operates with XRF spectrometers. The flexible and powerful Thermo Scientific™ OXSAS™ X-ray Fluorescence Analytical Software performs rapid, high-quality sample analysis using template-driven operations.Optional standardless quantitative analysis packages offer additional versatility for labs analyzing a wide range of materials. This platform evolves to meet changing needs with up-to-date solutions throughout the instrument's lifetime.<h3>⚙️ Ease of Use</h3><ul><li>Integrated Analytical Assistant guides method creation/extension, suggesting optimal parameters for fast and accurate analysis.</li><li>Automatic Method Choice selects the best parameter set without user intervention.</li><li>Routine analysis requires minimal data-entry (e.g., just sample ID).</li><li>One-click batch creation for all samples requiring analysis.</li><li>Simple batch definition and management allows sample prioritization.</li></ul><h3>🖥️ Familiar User Interface</h3><ul><li>Comprehensive post-treatment of results, including export and Excel support.</li><li>Integrated Microsoft SQL Server Express database stores setups and data.</li><li>Export/import functions simplify transfer of standards, methods, or grades between instruments.</li><li>Regular software updates available from the OXSAS website.</li><li>Monitoring and maintenance tools help maximize system uptime.</li><li>Real-time integrated SPC (Statistical Process Control).</li><li>SCT Manager provides overview of instrument and method capability.</li><li>Measurement Uncertainty and Conformance Probability reporting for unknowns.</li></ul><h3>⚡Fast Calibration</h3><ul><li>Multiple analyte curve display, instant base curve calculation, calibration templates.</li><li>Multi-variable regression and correction models with integrated theoretical alpha calculation.</li></ul><h3>🧩 Available Options</h3>Optional standardless analysis packages for broader capability:<ul><li>Thermo Scientific™ QuantAS™ – Semi-quantitative analysis.</li><li>Thermo Scientific™ UniQuant™ – Analyze up to 79 elements in unknown samples without specific calibration, no certified reference materials needed.</li></ul><h3>🔧 Other Options</h3><ul><li>ARLcom: Result transmission via LAN/TCP/IP, serial, or to files.</li><li>Remote Sample Definition: Sample registration from external networked applications.</li><li>Automation: Supports Thermo Scientific SMS for fully automated analysis.</li><li>Charge correction: Calculates material weights needed to adjust out-of-spec charges.</li><li>SPC-Full: Full graphical package for online Statistical Process Control.</li></ul><h3>✅ Compatible with:</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1211">Thermo Scientific™ ARL™ PERFORM'X Series</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1212">Thermo Scientific™ ARL™ OPTIM'X Series</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products?manufacturerItems=3&amp;search=9900">Thermo Scientific™ ARL™ 9900 IntelliPower Series</a></li></ul>

Products from category Software and libraries distributed by Thermo Fisher Scientific (EMEA) - page 1

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