Analysis of Bovine Liver using the Agilent 5100 Synchronous Vertical Dual View (SVDV) ICP-OES

Importance of the topic

Analysis of elemental composition in animal food products is critical for ensuring compliance with international regulations and safeguarding public health and the environment. Methods capable of simultaneous detection of nutrients and toxic elements with high sensitivity and throughput are essential for food testing laboratories.

Objectives and Study Overview

This study evaluates the performance of the Agilent 5100 Synchronous Vertical Dual View ICP OES for multi element analysis of a bovine liver standard reference material. Key aims include assessment of linear dynamic range, accuracy against certified values and method detection limits.

Methodology and Instrumentation

Sample Preparation and Standards

• Bovine liver SRM 1577A was digested by microwave in nitric acid and hydrogen peroxide and diluted to a fixed volume.
• Calibration standards ranged from low ppb to high ppm in acid medium and a 100 ppb spike was applied to evaluate trace recovery.

ICP OES System

• The instrument uses a vertical plasma torch and Dichroic Spectral Combiner to merge axial and radial views in one measurement.
• Sample introduction employed a SeaSpray nebulizer, cyclonic spray chamber and demountable torch.
• A solid state radio frequency generator ensured stable plasma operation.
• Operating conditions included defined read times, replicate scans, gas flows and pump rates optimized for robust performance.

Main Results and Discussion

The system achieved a linear dynamic range for sodium and potassium up to 500 ppm with correlation coefficients above 0.999 and calibration errors below ten percent. Measured concentrations for major and trace elements matched certified values within five percent. Method detection limits spanned low mg per kg levels. Spike recoveries for trace elements demonstrated accuracy between ninety nine and one hundred ten percent.

Benefits and Practical Applications

The approach enables simultaneous measurement of elements present at widely varying concentrations in a single run, reducing analysis time and argon consumption. The vertical torch design enhances robustness against complex matrices and extends maintenance intervals. Simplified software workflows and plug and play hardware support high sample throughput and reproducible results across operators and instruments.

Future Trends and Opportunities

Integration of this technology with automated sample handling and data analysis platforms will further increase laboratory efficiency. Advances in detector technology and software algorithms may expand the linear dynamic range and lower detection limits. Potential applications include environmental monitoring, clinical research and industrial quality control.

Conclusion

The Agilent 5100 Synchronous Vertical Dual View ICP OES with Dichroic Spectral Combiner offers a versatile and reliable solution for multi element analysis in food matrices. It combines high sensitivity, broad dynamic range and operational simplicity to meet the demands of modern analytical laboratories.

Reference

• Drvodelic N Analysis of Bovine Liver using the Agilent 5100 Synchronous Vertical Dual View ICP OES Agilent Technologies Publication 5991 4868EN July 2014