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Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer

 

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Application Note Biopharmaceuticals Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy Reproducible melting temperature (Tm) data at fast ramp rates using the Agilent Cary 3500 UV-Vis Authors Wesam Alwan and Mathieu Rault Agilent Technologies, Inc.…
Key words
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Application Note Biopharmaceuticals Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy Benefits of the Agilent Cary 3500 UV‑Vis spectrophotometer with a Peltier temperature‑controlled multicell in protein therapeutic research Authors Carlos Santos Martin La Trobe Institute for Molecular Science (LIMS), La…
Key words
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Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package Application Note Author Katherine Lighton, Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia. Mark J. Fiandaca, Boston Probes, Bedford, Massachusetts 01730,…
Key words
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Application Note Polymers Controlling the Swelling of Polymer Coated Gold Nanoparticles Impact of temperature and ionic strength on the cloud point, measured with a Cary 3500 Multizone Peltier UV-Vis spectrophotometer Author Ben Tadgell, Dr. Eser Akinoglu, Prof. Paul Mulvaney1 Dr.…
Key words
swelling, swellingaupnipam, aupnipamnanoparticles, nanoparticlestemperature, temperaturestrength, strengthionic, ioniccloud, cloudwavelength, wavelengthinduced, inducedinfluence, influenceinterval, intervalpoint, pointabsorbance, absorbancedeswelling, deswellingsalt
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