Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis SpectrophotometerTechnical notes | 2022 | Agilent Technologies InstrumentationUV–VIS spectrophotometryManufacturerAgilent TechnologiesKey wordscuvette, temperature, peltier, probe, block, nucleic, cuvettes, melting, stirring, sample, thermal, stranded, optimize, interval, melt, control, ramp, recommended, measurement, multicell, strands, buffer, acid, zone, measurements, absorbance, right, during, vis, configuration, averaging, data, space, nucleotides, cary, should, how, bandwidth, degassing, spectrophotometer, degas, zones, option, use, micro, between, condensation, avoid, ensure, bubbles
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Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy
2022|Agilent Technologies|Applications
Application NoteBiopharmaceuticalsCharacterization of Protein ThermalStability Using UV-Vis SpectroscopyBenefits of the Agilent Cary 3500UV‑Vis spectrophotometer with a Peltiertemperature‑controlled multicell in proteintherapeutic researchAuthorsCarlos Santos MartinLa Trobe Institute forMolecular Science (LIMS),La Trobe University,Melbourne, Victoria 3086,AustraliaBiswaranjan MohantySydney Analytical CoreResearch Facility,The University of Sydney,Sydney, New...
Key words
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The Basicsof UV-VisSpectrophotometryA primer Contents21Basic Principles of UV-Vis Measurement31.11.21.31.41.5The electromagnetic spectrumWavelength and frequencyUV-visible spectraTransmittance and absorbanceSummary333442How Does a Modern UV-Vis Spectrophotometer Work? 52.1Instrumental design3Selecting the Optimum Parameters for your UV-Vis Measurements133.13.23.33.43.53.63.73.83.93.10Optical cell selectionThermostatting your samplesStirring your sampleMeasurements at low temperaturesSolvent transparencyOptimum...
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Multiply YourExperimental PossibilitiesAgilent Cary 3500 UV-Vis Spectrophotometer Multiply Your Experimental ImpactThe innovative Agilent Cary3500 UV-Vis will transformyour laboratory.Designed from the groundup, it will streamline yourexperimental design andamplify confidence inyour results.The Cary 3500 UV-Vis will changethe way you:100˚–– Monitor enzymatic reactions attemperature––...
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Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy
2022|Agilent Technologies|Applications
Application NoteBiopharmaceuticalsFast Determination of Thermal MeltTemperature of Double-StrandedNucleic Acids by UV-Vis SpectroscopyReproducible melting temperature (Tm) data at fastramp rates using the Agilent Cary 3500 UV-VisAuthorsWesam Alwan andMathieu RaultAgilent Technologies, Inc. IntroductionDuring drug discovery, development,and manufacturing of nucleic acids,the Tm is often...
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ramp, ramptemperature, temperaturederivative, derivativesmoothing, smoothingmelt, meltmelting, meltingcary, carygξc, gξcsperm, spermherring, herringstranded, strandedrate, rateworkstation, workstationnucleic, nucleiccuvette