Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer

Technical notes | 2022 | Agilent TechnologiesInstrumentation
UV–VIS spectrophotometry
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Agilent Technologies
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Application NoteBiopharmaceuticalsCharacterization of Protein ThermalStability Using UV-Vis SpectroscopyBenefits of the Agilent Cary 3500UV‑Vis spectrophotometer with a Peltiertemperature‑controlled multicell in proteintherapeutic researchAuthorsCarlos Santos MartinLa Trobe Institute forMolecular Science (LIMS),La Trobe University,Melbourne, Victoria 3086,AustraliaBiswaranjan MohantySydney Analytical CoreResearch Facility,The University of Sydney,Sydney, New...
Key words
protein, proteintemperature, temperaturespectrophotometer, spectrophotometerpeltier, peltierthermal, thermalabsorbance, absorbancedsba, dsbaderivative, derivativemeasure, measuresmoothing, smoothingstability, stabilityvis, vismulticell, multicellecdsba, ecdsbatrobe
The Basics of UV-Vis Spectrophotometry
2021|Agilent Technologies|Guides
The Basicsof UV-VisSpectrophotometryA primer Contents21Basic Principles of UV-Vis Measurement31.11.21.31.41.5The electromagnetic spectrumWavelength and frequencyUV-visible spectraTransmittance and absorbanceSummary333442How Does a Modern UV-Vis Spectrophotometer Work? 52.1Instrumental design3Selecting the Optimum Parameters for your UV-Vis Measurements133.13.23.33.43.53.63.73.83.93.10Optical cell selectionThermostatting your samplesStirring your sampleMeasurements at low temperaturesSolvent transparencyOptimum...
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absorbance, absorbancewavelength, wavelengthvis, vislight, lightvisible, visiblespectrophotometer, spectrophotometermonochromator, monochromatorwavelengths, wavelengthskinetics, kineticscuvette, cuvettespectrophotometers, spectrophotometerssample, samplemeasurement, measurementintensity, intensityenergy
Application NoteBiopharmaceuticalsFast Determination of Thermal MeltTemperature of Double-StrandedNucleic Acids by UV-Vis SpectroscopyReproducible melting temperature (Tm) data at fastramp rates using the Agilent Cary 3500 UV-VisAuthorsWesam Alwan andMathieu RaultAgilent Technologies, Inc. IntroductionDuring drug discovery, development,and manufacturing of nucleic acids,the Tm is often...
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ramp, rampderivative, derivativetemperature, temperaturesmoothing, smoothingmelt, meltmelting, meltinggξc, gξccary, carysperm, spermstranded, strandedherring, herringrate, ratenucleic, nucleicworkstation, workstationinterval
Fluorescence measurement ofhybridization between quencher(DABCYL) labelled PNA probes and afluoresceine labelled DNA using theFluorescence BioMelt PackageApplication NoteAuthorKatherine Lighton,Agilent Technologies, Inc.Mulgrave, Victoria 3170,Australia.Mark J. Fiandaca,Boston Probes, Bedford,Massachusetts 01730, USA.IntroductionIn living cells, the process of reversible hybridization is a critical requirementto the...
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hybridization, hybridizationpna, pnathermodynamic, thermodynamicdna, dnathermal, thermalmelt, meltprobes, probesprobe, probeeclipse, eclipsedabcyl, dabcylpeltier, peltierfluorescence, fluorescencesoftware, softwarecary, carymulticell