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Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer

 

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Application Note Biopharmaceuticals Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy Reproducible melting temperature (Tm) data at fast ramp rates using the Agilent Cary 3500 UV-Vis Authors Wesam Alwan and Mathieu Rault Agilent Technologies, Inc.…
Key words
ramp, rampderivative, derivativetemperature, temperaturesmoothing, smoothingmelt, meltmelting, meltinggξc, gξccary, carysperm, spermherring, herringstranded, strandedrate, rateworkstation, workstationnucleic, nucleicinterval
Application Note Biopharmaceuticals Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy Benefits of the Agilent Cary 3500 UV‑Vis spectrophotometer with a Peltier temperature‑controlled multicell in protein therapeutic research Authors Carlos Santos Martin La Trobe Institute for Molecular Science (LIMS), La…
Key words
protein, proteintemperature, temperaturespectrophotometer, spectrophotometerpeltier, peltierthermal, thermalabsorbance, absorbancedsba, dsbaderivative, derivativemeasure, measuresmoothing, smoothingvis, visstability, stabilitymulticell, multicellecdsba, ecdsbatrobe
Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package Application Note Author Katherine Lighton, Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia. Mark J. Fiandaca, Boston Probes, Bedford, Massachusetts 01730,…
Key words
hybridization, hybridizationpna, pnathermodynamic, thermodynamicdna, dnathermal, thermalmelt, meltprobes, probeseclipse, eclipseprobe, probedabcyl, dabcylpeltier, peltierfluorescence, fluorescencesoftware, softwarecary, carymulticell
Application Note Polymers Controlling the Swelling of Polymer Coated Gold Nanoparticles Impact of temperature and ionic strength on the cloud point, measured with a Cary 3500 Multizone Peltier UV-Vis spectrophotometer Author Ben Tadgell, Dr. Eser Akinoglu, Prof. Paul Mulvaney1 Dr.…
Key words
swelling, swellingaupnipam, aupnipamnanoparticles, nanoparticlestemperature, temperatureionic, ionicstrength, strengthcloud, cloudwavelength, wavelengthinduced, inducedinfluence, influenceinterval, intervalpoint, pointabsorbance, absorbancedeswelling, deswellingsalt
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