Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy
Applications | 2022 | Agilent TechnologiesInstrumentation
UV–VIS spectrophotometry
IndustriesPharma & Biopharma
ManufacturerAgilent Technologies
Key wordsprotein, temperature, spectrophotometer, peltier, thermal, absorbance, dsba, derivative, measure, smoothing, stability, vis, multicell, ecdsba, trobe, cuvette, controlled, cary, dichroism, characterization, fluorescent, probes, drug, microcell, proteins, using, measurements, circular, block, therapeutic, workstation, agreed, discovery, averaging, melting, bandwidth, portions, disadvantages, wavelength, biochemical, can, properties, sample, six, needed, interval, biswaranjan, ellipticity, mohanty, quartz
Similar PDF
Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy
2022|Agilent Technologies|Applications
Application NoteBiopharmaceuticalsFast Determination of Thermal MeltTemperature of Double-StrandedNucleic Acids by UV-Vis SpectroscopyReproducible melting temperature (Tm) data at fastramp rates using the Agilent Cary 3500 UV-VisAuthorsWesam Alwan andMathieu RaultAgilent Technologies, Inc.IntroductionDuring drug discovery, development,and manufacturing of nucleic acids,the Tm is often...
Key words
ramp, rampderivative, derivativetemperature, temperaturesmoothing, smoothingmelt, meltmelting, meltinggξc, gξccary, carysperm, spermstranded, strandedherring, herringrate, ratenucleic, nucleicworkstation, workstationinterval
Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer
2022|Agilent Technologies|Technical notes
White PaperBest Practice for Nucleic AcidThermal Stability MeasurementsUsing the Cary 3500 UV-VisSpectrophotometerThermal melt (Tm) analysis using rapid, precisetemperature-dependent UV-Vis absorbancemeasurementsThe Cary 3500 Peltier UV-Vis systems offer the ability to analyze the thermalstability and profiles of biological and other molecules usingtemperature‑controlled...
Key words
cuvette, cuvettetemperature, temperaturepeltier, peltierprobe, probeblock, blocknucleic, nucleiccuvettes, cuvettesmelting, meltingstirring, stirringsample, samplethermal, thermalstranded, strandedoptimize, optimizemelt, meltinterval
Agilent Cary 3500 UV-Vis spectrophotometer series
2023|Agilent Technologies|Brochures and specifications
Multiply YourExperimental PossibilitiesAgilent Cary 3500 UV-Vis spectrophotometer seriesModular Design That SuitsYour Application NeedsThe Agilent Cary 3500 UV-Vis spectrophotometer series share a common UV-Visengine, which produces monochromatic light that is measured by the various UVVis sample measurement modules.This allows for the...
Key words
spectrophotometer, spectrophotometeropenlab, openlabsecure, secureabsorbance, absorbancecary, caryagilent, agilentvis, visdata, datastorage, storagecsv, csvspectrophotometers, spectrophotometerstemperature, temperatureesignatures, esignaturesworkstation, workstationyour
Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package
2011|Agilent Technologies|Applications
Fluorescence measurement ofhybridization between quencher(DABCYL) labelled PNA probes and afluoresceine labelled DNA using theFluorescence BioMelt PackageApplication NoteAuthorKatherine Lighton,Agilent Technologies, Inc.Mulgrave, Victoria 3170,Australia.Mark J. Fiandaca,Boston Probes, Bedford,Massachusetts 01730, USA.IntroductionIn living cells, the process of reversible hybridization is a critical requirementto the...
Key words
hybridization, hybridizationpna, pnathermodynamic, thermodynamicdna, dnathermal, thermalmelt, meltprobes, probesprobe, probeeclipse, eclipsedabcyl, dabcylpeltier, peltierfluorescence, fluorescencesoftware, softwarecary, carymulticell