Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy
Applications | 2022 | Agilent TechnologiesInstrumentation
UV–VIS spectrophotometry
IndustriesPharma & Biopharma
ManufacturerAgilent Technologies
Key wordsprotein, temperature, spectrophotometer, peltier, thermal, absorbance, dsba, derivative, measure, smoothing, stability, vis, multicell, ecdsba, trobe, cuvette, controlled, characterization, dichroism, fluorescent, probes, cary, drug, microcell, proteins, using, measurements, therapeutic, circular, block, workstation, discovery, agreed, melting, averaging, disadvantages, portions, bandwidth, biochemical, can, properties, sample, wavelength, six, needed, biswaranjan, ellipticity, mohanty, interval, changes
Similar PDF
Characterization of Biomolecules using High-Performance UV-Vis Spectrophotometry
2024|Agilent Technologies|Applications
Technical Overview Characterization of Biomolecules using High-Performance UV-Vis Spectrophotometry Accelerating research into oligonucleotides and proteins with the Agilent Cary 3500 UV-Vis Author Introduction Wesam Alwan Agilent Technologies, Inc. Molecular biology research plays a vital role in understanding biochemical and molecular…
Key words
vis, visspectroscopy, spectroscopyoligonucleotides, oligonucleotidesbiomolecules, biomoleculesstudies, studiesmelts, meltscharacterization, characterizationcan, cantemperature, temperatureenzyme, enzymeexperiments, experimentsthermal, thermalwavelength, wavelengthmeasure, measureactivity
Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer
2022|Agilent Technologies|Technical notes
White Paper Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer Thermal melt (Tm) analysis using rapid, precise temperature-dependent UV-Vis absorbance measurements The Cary 3500 Peltier UV-Vis systems offer the ability to analyze the thermal…
Key words
cuvette, cuvettetemperature, temperaturepeltier, peltierprobe, probeblock, blocknucleic, nucleiccuvettes, cuvettesmelting, meltingstirring, stirringsample, samplethermal, thermalstranded, strandedoptimize, optimizemelt, meltinterval
Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy
2022|Agilent Technologies|Applications
Application Note Biopharmaceuticals Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy Reproducible melting temperature (Tm) data at fast ramp rates using the Agilent Cary 3500 UV-Vis Authors Wesam Alwan and Mathieu Rault Agilent Technologies, Inc.…
Key words
ramp, rampderivative, derivativetemperature, temperaturesmoothing, smoothingmelt, meltmelting, meltinggξc, gξccary, carysperm, spermherring, herringstranded, strandedrate, ratenucleic, nucleicworkstation, workstationinterval
Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package
2011|Agilent Technologies|Applications
Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package Application Note Author Katherine Lighton, Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia. Mark J. Fiandaca, Boston Probes, Bedford, Massachusetts 01730,…
Key words
hybridization, hybridizationpna, pnathermodynamic, thermodynamicdna, dnathermal, thermalmelt, meltprobes, probesprobe, probeeclipse, eclipsedabcyl, dabcylpeltier, peltierfluorescence, fluorescencesoftware, softwarecary, carymulticell