Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy

Applications | 2022 | Agilent TechnologiesInstrumentation
UV–VIS spectrophotometry
Pharma & Biopharma
Agilent Technologies
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Application NoteBiopharmaceuticalsFast Determination of Thermal MeltTemperature of Double-StrandedNucleic Acids by UV-Vis SpectroscopyReproducible melting temperature (Tm) data at fastramp rates using the Agilent Cary 3500 UV-VisAuthorsWesam Alwan andMathieu RaultAgilent Technologies, Inc. IntroductionDuring drug discovery, development,and manufacturing of nucleic acids,the Tm is often...
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White PaperBest Practice for Nucleic AcidThermal Stability MeasurementsUsing the Cary 3500 UV-VisSpectrophotometerThermal melt (Tm) analysis using rapid, precisetemperature-dependent UV-Vis absorbancemeasurementsThe Cary 3500 Peltier UV-Vis systems offer the ability to analyze the thermalstability and profiles of biological and other molecules usingtemperature‑controlled...
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Agilent Cary 3500 UV-Vis spectrophotometer series
2023|Agilent Technologies|Brochures and specifications
Multiply YourExperimental PossibilitiesAgilent Cary 3500 UV-Vis spectrophotometer series Modular Design That SuitsYour Application NeedsThe Agilent Cary 3500 UV-Vis spectrophotometer series share a common UV-Visengine, which produces monochromatic light that is measured by the various UVVis sample measurement modules.This allows for the...
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Fluorescence measurement ofhybridization between quencher(DABCYL) labelled PNA probes and afluoresceine labelled DNA using theFluorescence BioMelt PackageApplication NoteAuthorKatherine Lighton,Agilent Technologies, Inc.Mulgrave, Victoria 3170,Australia.Mark J. Fiandaca,Boston Probes, Bedford,Massachusetts 01730, USA.IntroductionIn living cells, the process of reversible hybridization is a critical requirementto the...
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