ICPMS
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike
Author
Ústav organické chemie a biochemie AV ČR
The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry.
Tags
Article
Various
Video
Logo of LinkedIn

Researchers at IOCB Prague develop a new method for enzymatic synthesis of potential RNA therapeutics

Mo, 22.4.2024
| Original article from: ÚOCHB AV ČR/Veronika Sedláčková
A team of researchers at IOCB Prague led by Prof. Michal Hock has developed a novel method for preparing ribonucleic acid (RNA) containing modified bases.
Video placeholder
  • Photo: IOCB Prague/Tomáš Belloň: Scientists at IOCB Prague develop a new method for enzymatic synthesis of potential RNA therapeutics
  • Video: IOCB Prague: Scientists at IOCB Prague develop a new method for enzymatic synthesis of potential RNA therapeutics

A team of researchers at IOCB Prague led by Prof. Michal Hocek has developed a novel method for preparing ribonucleic acid (RNA) containing modified bases. Innovative use of engineered DNA polymerases, enzymes commonly used for synthesis of DNA, led to development of a general method for the synthesis of RNA modified only at selected sites or even RNA containing various modifications at all nucleotide building blocks. This opens the door to applications in chemical biology and, in a longer perspective, in the therapy of hitherto incurable diseases. The paper was published in the journal Nature Communications.

The researchers used two artificially modified DNA polymerases known to be capable of synthesizing RNA. In nature, DNA polymerases synthesize only DNA, while RNA polymerases produce RNA. Hocek’s team has developed a procedure for preparing modified RNA with significant advantages over the commonly available method, in vitro transcription with viral T7 RNA polymerase, which, for example, is used in the production of well-known mRNA vaccines.

Modified DNA polymerases can incorporate virtually any modification into any RNA sequence, even only at selected specific sites, precisely where the given modification is required. The commonly used T7 RNA polymerase can only incorporate the modified base in all positions in the RNA but not in one precisely designated location. Therefore, if the RNA requires site-specific modification, the classical in vitro transcription fails and a different method is needed.

And that’s exactly what the team at IOCB has developed, a method that in many respects is comparable to the commonly used in vitro transcription while eliminating most of its pitfalls and offering completely new possibilities. Now, various specific RNA probes can be prepared for the study of RNA biology, which is currently a very hot topic. In the longer term, however, there is great promise for therapeutic applications, especially for mRNA therapeutics.

IOCB - Researchers at IOCB Prague develop a new method for enzymatic synthesis of potential RNA therapeutics

The researchers chose two specific positions in mRNA for modifications and found that they led to a significant increase in the production of certain proteins. This is an excellent news for the development of potential mRNA drugs. If mRNA modified in this way could be introduced into cells, it would be possible to trigger the production of a protein which the body lacks or which is defective.

“Our method may lead to the development of therapeutics for the treatment of many diseases, including cancer and some genetic diseases caused by a malfunctioning or missing protein. It allows for the replacement of a missing or poorly functioning protein,” says Michal Hocek. He continues: “RNA therapy is a powerful technology, and it may emerge as one of the main directions of drug development within the next ten years.”

IOCB Prague/Tomáš Belloň: Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids group at IOCB Prague

The challenge is how to ensure that there is just the right amount of protein – not scarce and neither excessive – at just the right time. In most cases, if there is too much of certain proteins, they are harmful to the body. That’s why mRNA naturally breaks down quickly in the cell and the entire process is very finely regulated by the body. According to Michal Hocek, their method will not replace the classical in vitro transcription. However, if there is a need for specific modifications to occur only at selected RNA sites, then the advantage will lie with the novel method from IOCB Prague.

Ústav organické chemie a biochemie AV ČR
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
Other projects
Follow us
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike