ICPMS
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike
Author
Ústav organické chemie a biochemie AV ČR
The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry.
Tags
Article
Scientific article
Logo of LinkedIn

Insight into the nucleosome recognition mechanisms

We, 17.4.2024
| Original article from: IOCB Prague
Scientists from IOCB Prague, Charles University, CEITEC, the Institute of Microbiology, and the University of Cologne studied the chromatin reader LEDGF and its association with methylated histone H3K36.
- **Photo:** IOCB PRague: Insight into the nucleosome recognition mechanisms
  • Photo: IOCB PRague: Insight into the nucleosome recognition mechanisms

In the eukaryotic nucleus, DNA is wrapped around highly basic proteins called histones thereby forming a basic DNA storage unit – the nucleosome. Histone covalent modifications (methylation) represent an essential mechanism for the regulation of expression of specific genes in living organisms. Small changes in this highly regulated system, occurring in an abnormal position or place, often lead to disease. However, the molecular mechanisms of the recognition of various states of particular marks are not entirely understood.

Scientists led by Václav Veverka from IOCB Prague and Charles University, together with colleagues from CEITEC, the Institute of Microbiology of the CAS, and the University of Cologne studied the chromatin reader LEDGF and its association with methylated histone H3K36, which is known as a marker of active transcription.

The researchers employed cryo-EM coupled with NMR spectroscopy to dissect both structured and dynamic regions of LEDGF protein involved in reading the methylated nucleosomes.

They have structurally validated that the readout of both di- and trimethylated nucleosomes is mechanistically versatile.

The structural conservation of differently methylated histones might not be as surprising, but we have also identified an alternative binding site for LEDGF on a nucleosome, independent of the degree of methylation.
  • Eliška Koutná, the first author of the study

Moreover, with NMR they have also discovered that disordered regions in LEDGF that interact with DNA preferentially interact with extranucleosomal DNA but are still flexible in the context of dinucleosome.

ÚOCHB - Insight into the nucleosome recognition mechanisms - Structure of the PWWP domain from LEDGF bound to H3K36 trimetylated dinucleosome.jpg

These results were published in the journal Nucleic Acids Research with Eliška Koutná as the first author.

Resources

Koutná, E.; Lux, V.; Kouba, T.; Škerlová, J.; Nováček, J.; Srb, P.; Hexnerová, R.; Šváchová, H.; Kukačka, Z.; Novák, P.; Fábry, M.; Poepsel, S.; Veverka, V. Multivalency of nucleosome recognition by LEDGF. Nucleic Acids Res. 2023. https://doi.org/10.1093/nar/gkad674

Ústav organické chemie a biochemie AV ČR
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

FTIR Series and Infrared/Raman Microscope Accessories

Brochures and specifications
| 2024 | Shimadzu
Instrumentation
FTIR Spectroscopy, RAMAN Spectroscopy, Microscopy
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

For TOC Analyzers Shimadzu 40 mL Vial LE

Others
| 2024 | Shimadzu
Instrumentation
TOC, Consumables
Manufacturer
Shimadzu
Industries
Others

Transmission Measurements of Solid Samples at Variable Angles of Incidence by UV-Vis

Applications
| 2024 | Agilent Technologies
Instrumentation
UV–VIS spectrophotometry
Manufacturer
Agilent Technologies
Industries
Materials Testing

Utilizing hyphenated EC-Raman to study a model system

Applications
| 2024 | Metrohm
Instrumentation
RAMAN Spectroscopy
Manufacturer
Metrohm
Industries
Energy & Chemicals
 

Related articles


Article | Scientific article

Cryo-EM structures show how Trypanosomes escape the human innate immune response

Researchers from IOCB Prague determined the first cryo-EM structures of a surface receptor of the human parasite T. brucei gambiense in complex with human complement factor C3 in different conformations.
Ústav organické chemie a biochemie AV ČR
more

Article | Scientists

Gerrit Angst awarded the Lumina quaeruntur premium. He will start a new research team

Soil biologist Gerrit Angst from the Biology Centre of the CAS received the prestigious Lumina quaeruntur Premium from the President of the Czech Academy of Sciences.
Akademie věd České republiky
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
Other projects
Follow us
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike