<div><p><strong>In 2025, two outstanding young female researchers, Drs. Filipa Oliveira and Stella Gonsales, both originally from outside the Czech Republic, received prestigious JUNIOR STAR grants from the Czech Science Foundation (GAČR). JUNIOR STAR enables early career researchers to establish their own independent research groups and significantly advance their scientific careers. Their projects reflect cutting-edge research in materials science and sustainable chemistry, highlighting the international reach and welcoming research environment at UCT Prague.</strong></p><p><strong>Dr. Stella Gonsales is also a&nbsp;laureate of the Dagmar Procházková Fund, which supports promising scientific projects in sustainable and advanced organic chemistry. Another recipient of this grant is Dr.&nbsp;Anna Valunchene, whose research complements UCT Prague’s&nbsp;sustainability goals. This dual funding underlines Stella’s&nbsp;remarkable achievements and the wide scope of innovative research at UCT Prague.</strong></p><p><strong>Both Filipa and Stella are actively looking for motivated students and researchers to join their teams, offering a&nbsp;unique opportunity to work at the forefront of science in a&nbsp;vibrant and supportive environment.</strong></p></div><div><h3><strong>Interview with Filipa Oliveira: Pioneering tubular MXenes for supercapacitor energy storage</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Filipa Oliveira" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg 235w," sizes="100vw" width="1500" height="999"></p><h5><strong>Congratulations on receiving the JUNIOR STAR grant. What does this achievement mean for you and how will it influence your next steps?</strong></h5><p>Thank you. It’s&nbsp;a huge achievement to receive this prestigious grant. It gives me the opportunity to establish my own research group here at UCT Prague. The JUNIOR STAR program supports talented young scientists in building their teams and accessing resources. This means I&nbsp;now have the support to focus fully on my research about tubular MXenes and their application in supercapacitors.</p><h5><strong>Why are tubular MXenes so innovative for energy storage?</strong></h5><p>MXenes are a family of 2D materials with a layered, accordion-like structure and known for their excellent electrical conductivity. Our innovation is to produce them with a tubular morphology, combining the high conductivity and redox activity in superior ion-transport tubular architectures. Their open, hollow structure increases surface area, ion diffusion and charge transport. This could translate to faster, more efficient supercapacitors, critical for sustainable energy technologies.</p><h5><strong>How did your scientific interest lead you to this field?</strong></h5><p>Global challenges around green energy made me focus on materials that can improve energy storage. MXenes stood out because changes in the composition and structure can significantly change their properties. I&nbsp;was curious about how tuning different metallic elements in a&nbsp;tubular morphology can impact how MXenes perform and how to optimize them for practical applications.</p><h5><strong>What challenges have you encountered?</strong></h5><p>One major challenge is their stability: MXenes oxidize quickly which deteriorates performance. Another challenge is understanding how synthesis conditions of both MXenes and their precursors (MAX phases) must be adapted to create and preserve tubular morphologies since their synthesis mechanisms differ from conventional MXenes. Additionally, effectively communicating the project in the funding proposal to show to reviewers why I&nbsp;should obtain this funding was also a&nbsp;challenge, but thanks to mentorship and collaboration, I&nbsp;overcame these hurdles.</p><h5><strong>What motivates you most about working at UCT Prague?</strong></h5><p>The support from colleagues and the excellent technical infrastructure make it a&nbsp;great place to work. Also, being a&nbsp;former member of Professor Sofer’s&nbsp;leading group in 2D&nbsp;materials is inspiring and led me to this achievement.</p><h5><strong>How do&nbsp;you see your professional development going in the future?</strong></h5><p>The grant will help me build my own team, acquire equipment, and develop collaborations. My long-term vision is to contribute significantly to sustainable energy solutions and scientific progress both locally and internationally.</p><h5><strong>You are looking for new team members. What kind of students or scientists do&nbsp;you need?</strong></h5><p>We are currently welcoming enthusiastic master's&nbsp;students, PhD candidates, and postdocs who are passionate about materials science and eager to explore new frontiers in MXenes and energy storage. If you love curiosity-driven research and want to be part of a&nbsp;growing international team, we encourage you to join us.</p><h3><strong>Interview with Stella Gonsales: Catalysis as a&nbsp;key to polymer recycling</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Stella Gonsales" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg 233w," sizes="100vw" width="1499" height="1000"></p><h5><strong>Congratulations on your JUNIOR STAR grant and also being awarded the Dagmar Procházková Fund. What does receiving both mean to you and how will they shape your work?</strong></h5><p>Thank you. Both grants are hugely important. The JUNIOR STAR grant allows me to expand my group and resources for research into catalytic recycling of polymers, while the Dagmar Procházková Fund supports complementary projects such as synthesizing new biodegradable polymers. Together, they help tackle the plastic waste problem from multiple scientific angles.</p><h5><strong>Could you explain your research in layperson’s&nbsp;terms?</strong></h5><p>Most plastics have strong carbon-carbon bonds. We insert more cleavable double bonds into polymers that can be broken down by catalytic reactions called olefin metathesis, making recycling on a&nbsp;molecular level more feasible.</p><h5><strong>What makes the JUNIOR STAR grant particularly important for your research and career development?</strong></h5><p>The JUNIOR STAR grant is a&nbsp;prestigious and highly competitive funding opportunity aimed at excellent early-career scientists within eight years of completing a&nbsp;Ph.D. It provides up to 25&nbsp;million CZK over five years, allowing recipients to establish independent research groups and pursue innovative scientific topics. This grant is crucial for building a&nbsp;solid foundation, acquiring necessary equipment, and recruiting talented team members, enabling the pursuit of ambitious projects that can significantly contribute to both the Czech scientific community and global challenges.</p><h5><strong>What collaborations support your research?</strong></h5><p>The collaboration between UCT Prague and the Institute of Organic Chemistry and Biochemistry (IOCB) of the Czech Academy of Sciences primarily provides financial and technical support. The cooperation helps with funding, access to advanced equipment, and operational resources needed for running research projects.</p><h5><strong>What advice would you give young scientists aiming to succeed?</strong></h5><p>I&nbsp;encourage thinking outside the box and combining diverse knowledge you acquire during your career to address complex problems that are exciting and relevant to you.</p><h5><strong>Your laboratory bears the name of Otto Wichterle and was ceremonially opened in the presence of Professor Kamil Wichterle. How does Wichterle’s&nbsp;legacy inspire you personally?</strong></h5><p>Wichterle’<s>’</s>s&nbsp;innovative spirit motivates me to push creative boundaries and pursue novel solutions in sustainable chemistry.</p><h5><strong>What are your visions for the future?</strong></h5><p>I&nbsp;aim to build a&nbsp;research group that advances sustainable polymer recycling, contributing to environmental responsibility and the circular economy.</p><h5><strong>You are recruiting people for your team. What skills do&nbsp;they need to have?</strong></h5><p>We are looking for talented Ph.D. students and postdocs experienced in air-sensitive catalysis techniques. If you want to contribute to transformative research in sustainable chemistry and join a&nbsp;dynamic team, this is a&nbsp;great opportunity.</p></div>

JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science

<p><strong>The clean laboratories of the CEITEC Nano research infrastructure at CEITEC Brno University of Technology house a four-meter-tall TITAN transmission electron microscope for researching materials at the atomic level. This scientific device underwent two significant upgrades this year, entailing the addition of two state-of-the-art technologies: an electromagnetic lens aberration corrector for scanning transmission electron microscopy and an electron energy loss spectrometer equipped with cameras featuring today's most sensitive sensors. Thanks to these upgrades, TITAN has become a state-of-the-art device that can only be found in a few of the world's most advanced laboratories in this configuration.</strong></p><p><strong>The TITAN Themis microscope </strong>was manufactured in 2015 by FEI (now Thermo Fisher Scientific) and has been in use at CEITEC BUT since 2016. At the time of its acquisition, it was one of<strong> the most powerful commercially available electron microscopes</strong>; however, it lacked a corrector for optical defects in the lenses that form the narrow electron beam scanning the sample. This meant limited resolution and a lower ability to analyze the chemical composition and physical properties of materials at the atomic level.</p><p><i>"The latest generation S-CORR aberration corrector allows the electron beam to be focused to a size up to twice smaller than an atom, while also providing a higher electron current. This has led to a significant increase in the resolution and analytical capabilities of the microscope,"</i> explains <strong>Jan Michalička</strong>, guarantor of the TITAN microscope. "<i>Thanks to the new corrector, our microscope achieves the world's highest standard in resolution, specifically less than 50 picometers at an acceleration voltage of 300 keV." </i>For better understanding, he adds a comparison: <i>"Imagine the ratio of the size of a golf ball to that of planet Earth. That is exactly the ratio of an atom measuring 100 pm to a golf ball."</i> Thanks to this resolution, scientists are not only able to image individual atoms, but also to determine their chemical nature through further analysis. This is of fundamental importance, for example, for the development of new catalysts for a more environmentally friendly chemical industry.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Differences in resolution before and after the installation of the aberration corrector." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8ebd8d7245.jpg 245w," sizes="100vw" width="1600" height="904"></p><p>The new corrector also allows the microscope to achieve high resolution even with low-energy electrons. This is crucial for studying highly sensitive materials such as graphene and other two-dimensional structures, which would be damaged by a stronger electron beam. <i>"Electrons interact strongly with the sample and can damage it at the atomic level, for example, by changing its structure and chemical composition. With the new corrector, we can achieve atomic resolution even at the lowest acceleration voltage of 60 keV, allowing us to study even very sensitive materials in a virtually unchanged state," </i>says Jan Michalička.</p><h4><strong>The additional installation of a corrector at the CEITEC laboratory is unique worldwide</strong></h4><p><strong>Correctors of optical defects in electromagnetic lenses are an exceptional technology</strong> that has completely changed the world of electron microscopy. For a long time, they were considered technically impossible, and the absence of correctors essentially prevented research into the structure and properties of materials at the atomic level. It was not until the 1990s that a group of four scientists – Harald Rose, Max Haider, Knut Urban, and Ondřej Křivánek – succeeded in implementing this technology, for which they received the prestigious Kavli Prize in 2020. <i>“Correctors basically consist of a pair of complex lenses, each containing a series of magnetic multipoles. In the case of the correctors invented by the first three scientists mentioned above, defects are removed by two hexapole lenses. Our new corrector is based on the same concept,”</i> adds Jan Michalička.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Delivered aberration corrector prior to installation, with the TITAN microscope in the background." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_2_c7e74d8554.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><p>The additional installation of a corrector is <strong>unique </strong>in itself nowadays. Similarly complex technologies are usually installed during the manufacture of microscopes in the factory, in this case in the Netherlands. However, in Brno, the microscope was successfully upgraded with a corrector under the limited conditions of the laboratory. The installation of the corrector took about four months and required the dismantling of a large part of the microscope. <i>"With this installation, we basically went back to the time when the four scientists mentioned above placed their prototype corrector on an older microscope, thus improving its performance,"</i> says Jan Michalička with a smile, adding that the most difficult part was the precise adjustment of the corrector and the entire optics of the microscope in order to achieve top parameters. <i>"Even though most things are now controlled by computers, there are only a few people in the world who can install and tune a corrector to the highest level of accuracy. We are therefore extremely pleased to have had such experts at our disposal here."</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the aberration corrector – removal of the condenser." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_4_b2cf95fb5c.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><h4><strong>New spectrometer will enable scientists to see more</strong></h4><p>The second and equally important upgrade to the TITAN microscope is the installation of <strong>the latest generation GIF Continuum HR electron energy loss spectrometer by Gatan</strong>. This is an approximately two-meter-long device installed horizontally under the microscope, whose main component is a magnetic prism that separates the electron beam according to their energy losses. The spectra of these losses provide enormous information about the sample, principally at the atomic level, as they are characteristic of the given state of the atoms, such as the valence arrangement of their electron shell. In combination with the new corrector, the spectra can be used to analyze the chemical bonds, arrangement in the crystal lattice, or the entire range of physical properties of atomic nanostructures down to the level of individual atoms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installation of the spectrometer beneath the microscope, magnetic prism." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_8_fdab871ef5.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><p>An essential part of the spectrometer are the <strong>state-of-the-art K3 and Stela cameras</strong>, which can directly detect and count individual electrons. At the same time, these cameras are extremely fast and their sensors capture data without noise. <i>"These features of our cameras are key to measuring sensitive or dynamically changing samples and allow us to obtain information that was previously invisible to electron microscopes,"</i> says Jan Michalička.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Installed high-performance spectrometer cameras." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_9_d8b87903f2.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><p>Last but not least, the spectrometer will open up completely new possibilities for measuring the magnetic properties of atomic structures. The new spectrometer will also be used for such experiments within the project TERAFIT, which financed this upgrade, and which focuses on research into materials for ultra-high capacity, speed, and energy efficiency in information technology. <i>"The device is fully prepared for advanced experiments and the use of modern software tools, including artificial intelligence,"</i> adds <strong>Vojtěch Uhlíř</strong>, head of the <i>Nanomagnetism and Spintronics</i> research group at CEITEC BUT and one of the TERAFIT project investigators, highlighting the advantages of the spectrometer.</p><p>Together with the corrector, the spectrometer represents a breakthrough in the analysis of material properties, and according to Jan Michalička, CEITEC now has one of the most powerful microscopes available. <i>“In modern materials science, for example, in the development of magnetic memories, quantum materials, or 2D structures, it is necessary to see not only atoms, but also their behaviour in real time, including magnetic states. And it is this spectrometer that pushes the boundaries of what we can see and analyze.</i>” Michal Urbánek, head of the CEITEC Nano laboratories and coordinator of the large research infrastructure <a target="_blank" rel="noopener noreferrer" href="https://www.czechnanolab.cz/"><strong>CzechNanoLab</strong></a>, agrees with him, adding that the device can now contribute to scientific knowledge on a global scale. <i>“Thanks to its integration into the large CzechNanoLab research infrastructure, this state-of-the-art microscope is accessible not only to scientists from CEITEC, but also to other institutions in the Czech Republic and abroad.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg" alt="CEITEC/CEITEC BUT archive: Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories: Final configuration of the TITAN microscope after installation of the aberration corrector and spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Upgrade_transmisniho_elektronoveho_mikroskopu_TITAN_zaradil_CEITEC_mezi_elitni_laboratore_10_7a2b57cc6d.jpeg 117w," sizes="100vw" width="1600" height="2133"></p><blockquote><p>Do you want to know more? Read the<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/upgrade-of-titan-transmission-electron-microscope-ranks-ceitec-among-elite-laboratories-expert-version/t11696?secret=40ab0267-ec7e-11f0-8f5d-2ed9ab825d4b"><strong>expert version</strong></a><strong> </strong>of this article.</p></blockquote>

Upgrade of TITAN transmission electron microscope ranks CEITEC among elite laboratories

<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

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C&EN

<p><strong>Is your raw material identification (ID) testing process optimized? Can it handle a high volume of receipts without stretching your QC budget? If you use a sampling booth and/or open papers sacks, plastics buckets/drums, or other commonly used pharmaceutical containers to ID excipients, API, or other raw materials, your ID process can be improved.</strong></p>
<p>This webinar will describe how raw material verification testing is now possible through transparent and non-transparent packaging directly in quarantine using Spatially Offset Raman Spectroscopy (SORS). The Agilent Vaya Raman is the first handheld spectrometer incorporating SORS to quickly identify raw materials directly through containers in a cGMP environment. This webinar will also discuss SORS technology, its impact on the ID test process, and subsequent return on investment (ROI) against current ID testing solutions.</p>
<h5><strong>Key learning objectives</strong></h5>
<ul>
<li>Understand how raw material identity verification through transparent and non-transparent containers removes the need for sampling, increasing testing throughput without increasing costs</li>
<li>Learn how the Vaya system, with true through-barrier SORS technology, works through most common containers – from clear glass vials to multilayer paper sacks</li>
<li>Learn how Vaya improves current testing protocols – from low volume sampling to 100% ID testing</li>
<li>Discover the ROI against classic conventional Raman handhelds and NIR systems</li>
<li>Understand how to streamline an identification process with Vaya</li>
</ul>
<h5><strong>Who should attend?</strong></h5>
<ul>
<li>QC lab managers, analysts, and scientists</li>
<li>Raw material managers</li>
<li>Plant managers</li>
<li>Operation efficiency managers</li>
<li>Continuous improvement managers</li>
<li>Vice Presidents of Quality and Operations</li>
<li>PAT scientists</li>
</ul>
<blockquote>
<p><strong>Presenter: Frederic Prulliere (Raman ID Product Manager, Agilent Technologies)</strong></p>
</blockquote>
<blockquote>
<p>Frederic Prulliere is the Product Manager for the Raman ID product line at Agilent Technologies. Prulliere leads the product development of spatially offset Raman spectroscopy-based solutions, particularly for verifying raw materials at reception. He joined Agilent Technologies in 2012 as a business development manager for mobile Fourier transform infrared spectroscopy (FTIR).</p>
</blockquote>


SelectScience: Rapidly scan your raw materials with SORS

Rapidly scan your raw materials with SORS

We will describe how raw material verification testing is now possible through transparent and non-transparent packaging directly in quarantine using SORS.

<p><strong>We’ll explore polymer characterization, including quick polymer identification for recycling purposes, identifying product defects or failures for quality control, reverse engineering to facilitate product development. We’ll dive into polymer research – an active area of scientific endeavor where performance and a reduced environmental impact are key elements of interest. Learn why vibrational spectroscopy (infrared (IR) and Raman) instruments are useful for the analysis of polymers in an industrial setting, as IR and Raman are highly specific and sensitive tools that are non-destructive and easy to use.</strong></p>
<p>During the session, we’ll offer clear examples that demonstrate the effectiveness of IR and Raman analysis in each of these categories.</p>
<h5><strong>Key webinar take-aways:</strong></h5>
<ul>
<li>The importance of polymer research where performance and a reduced environmental impact are key elements of interest.</li>
<li>Utilizing vibrational spectroscopy (infrared (IR) and Raman) as a useful tool for the analysis of polymers in an industrial setting.</li>
<li>IR and Raman are highly specific and sensitive tools that are non-destructive and easy to use.</li>
<li>Examples will be presented demonstrating the effectiveness of IR and Raman analysis in each of these categories.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Tom Tague (Applications Manager, Bruker Corporation)</p>
</blockquote>
<blockquote>
<p>Dr. Tom Tague is the Applications Manager for Bruker Optics. He is also a member of the Visiting Advisory Committee of the Metropolitan Museum of Art in New York and the Strategic Advisory Board of Amplified Sciences. Dr. Tague received his Ph.D. from the University of Utah in Physical Chemistry and his B.S. also in Chemistry from the University of Texas at San Antonio. He also conducted his postdoctoral research at the University of Virginia working with Professor Lester Andrews. He is a member of the American Chemical Society, Society for Applied Spectroscopy, American Physical Society, and the Optical Society of America. Dr. Tague is active in developing new methods and instrumentation with the goal of improving the sensitivity and detection limits of spectroscopy related applications. Tom has more than 90 publications and 5 Patents.</p>
</blockquote>


Pixabay/Adrian Malec: Polymer Science A to Z: Getting Answers from FTIR and Raman Analyses

Polymer Science A to Z: Getting Answers from FTIR and Raman Analyses

Let’s tackle the science of polymer analysis using the power of spectroscopy, including identification, quality control and reverse engineering. 

Polymer Science A to Z: FTIR and Raman Analyses

<p><strong>Transmission Raman spectroscopy (TRS) is a variant of Raman spectroscopy which is advantageous in probing bulk content of diffusely scattering samples.  Spatially offset Raman spectroscopy (SORS) is a variant of Raman spectroscopy that allows highly accurate chemical analysis of objects beneath obscuring surfaces.  TRS is used in Agilent TRS100 Raman system for fast assay of tablets, capsules, and other dosage forms.  SORS is used in Agilent RapID and Vaya systems for see-through barrier verification applications.</strong></p>
<p>This online seminar provides a comprehensive introduction to the TRS and SORS technologies and their applications.</p>
<blockquote>
<p><strong>Presenter:</strong> Yanqia Wang, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Dr. Yanqia Wang started working for Agilent Technologies Inc. as a FTIR application Engineer in 2013, providing pre-&amp;post-sale application support. The products he covers from Bench-top FTIR microscopes to various Mobile FTIR spectrometers. Dr. Wang received his PhD of analytical chemistry from Duke University in 2004, doing dynamic IR spectroscopy research. Then he joined Fitzpatrick Center for Photonics at Duke University, doing Tissue Raman spectrometer instrumentation. Since 2007, Dr. Wang worked for Avery Dennison Corp. as a spectroscopist, providing molecular analytical support to the research &amp; development.</p>
</blockquote>


Agilent Technology: Fast assay of Tablets, Capsules, and other Dosage forms using Transmission Raman Spectroscopy and SORS Raman Spectroscopy

Fast assay of Tablets, Capsules, and other Dosage forms using Transmission Raman Spectroscopy and SORS Raman Spectroscopy

This online seminar provides a comprehensive introduction to the TRS and SORS technologies and their applications.

Tablets, Capsules by Raman Spectroscopy and SORS

<p>IR and Raman spectroscopy are well established, mature techniques present nowadays in most analytical laboratories.  However, they do pose several key fundamental limitations. The new breakthrough technique of Sub-micron Optical Photothermal Infrared (O-PTIR) Spectroscopy overcomes the limited spatial resolution of traditional IR microscopy with submicron resolution and the limited sensitivity and fluorescence interference limitations of Raman microscopy. </p>
<p>Furthermore, O-PTIR is able to bring together the complementarity of IR and Raman into a single platform, single measurement for the first time, enables sub-micron, simultaneous IR+Raman microscopy delivering IR and Raman spectra from the same spot, at the same time with the same spatial resolution. </p>
<p>This webinar discusses this novel technique and provides numerous application examples.</p>
<h4><strong>Part 1: Introduction &amp; Overview by Dr. Mustafa Kansiz</strong></h4>
<ul>
<li>Overview of current direct IR (FTIR &amp; QCL) and Raman micro spectroscopic techniques and Fluorescence Microscopy </li>
<li>Introduction to Submicron O-PTIR and simultaneous Raman microscopy (IR+Raman) and the benefits of co-located Fluorescence </li>
<li>Various application examples of particulates, materials science and more</li>
</ul>
<h4><strong>Part 2: Review of Environmental Applications by Dr. Andrew Ault Microplastics, environmental</strong></h4>
<p>Dr. Ault will present the results of his group&#39;s recent publication on an important source of atmospheric particles - Sea Spray Aerosols (SSA).  </p>
<p>In this work, they characterize individual SSA particles using new generation submicron simultaneous IR+Raman (O-PTIR) microscopy for organic profiling, combined with EDX for elemental profiling. </p>
<p>Dr. Ault will also talk to his recent research relating to microplastics.  </p>
<h5>Key Learning Objectives:</h5>
<ul>
<li><strong>Understand the principle of operation of O-PTIR for submicron IR spectroscopy</strong></li>
<li><strong>Learn about specific applications of submicron simultaneous IR and Raman</strong></li>
<li><strong>How O-PTIR can advance your research goals and increase the impact of your publications</strong></li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Laboratory Directors and Managers  </li>
<li>Research Professors and Academics</li>
<li>Regional Water Board Researchers</li>
<li>Industrial Failure Analysis Engineers</li>
<li>Chemical and Polymer Engineers and Scientists</li>
<li>Vibrational Spectroscopists</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Mustafa Kansiz (Director of Product Management and Marketing, Photothermal Spectroscopy Corp.)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Andrew Ault (Dow Corning Assistant Professor of Chemistry and Associate Professor of Chemistry, University of Michigan)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)</p>
</blockquote>


C&EN: Sub-500nm Simultaneous IR+Raman Microscopy for Microplastics and Materials Science Research

Sub-500nm Simultaneous IR+Raman Microscopy for Microplastics and Materials Science Research

This webinar discusses this novel technique and provides numerous application examples.

Sub-500nm Simultaneous IR+Raman Microscopy for Microplastics

<p><strong>Raman spectroscopy is used around the globe for identification of unknowns, verification of raw materials, basic R&amp;D, and process monitoring and control. The most commonly deployed technology is based on 785 nm lasers and detectors that leverage innovations from the telecommunications boom in the 1990s. However, this technology has been fundamentally limited by fluorescence interference that overpowers the Raman signal. The most common ways to overcome fluorescence have all had significant drawbacks around cost, safety and sample preparation – until now.</strong></p>
<p>Developed for first responders to help identify unknowns in street narcotics, eXTRaction (XTR) technology uses advanced digital signal processing to separate Raman data from a background. With XTR, 785 nm Raman can be extended to difficult-to-analyze systems.</p>
<p>In this webinar, Dr. Adam J. Hopkins and Elena Hagemann will explain how XTR works and show different use cases for both qualitative and quantitative measurements, including biodiesel and polyurethane production.</p>
<h5>Who should attend?</h5>
<ul>
<li>QA departments looking to extend raw material identification outside the lab</li>
<li>Researchers looking for flexible Raman measurement solutions for a variety of materials</li>
<li>Anyone measuring complex media that is too fluorescent for 785 nm Raman spectroscopy</li>
<li>Process development engineers and scientists looking for upstream/development solutions that can scale with production</li>
</ul>
<h5>Key learning points</h5>
<ul>
<li>XTR processing enables Raman measurements of fluorescent materials</li>
<li>Quantitative measurement performance is not adversely impacted by XTR processing</li>
<li>Raw material ID performance is enhanced with XTR, with identification performance comparable to 1064 nm systems</li>
<li>Non-contact Raman measurements are possible from up to 1.5 meters with XTR enabled systems</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Adam J. Hopkins, PhD (Spectroscopy Product Manager, Metrohm USA)</p>
</blockquote>
<blockquote>
<p>Dr. Adam J. Hopkins is the Spectroscopy Product Manager at Metrohm USA. He has nearly 20 years of experience as a vibrational spectroscopist. He received his Ph.D. in chemistry from the University of Oregon where he studied nonlinear optical spectroscopy under Geraldine Richmond.</p>
</blockquote>
<blockquote>
<p>Prior to joining Metrohm USA, Adam worked developing spectroscopic solutions for standoff and noncontact sensing applications using Laser Induced Breakdown and Raman spectroscopy. Dr. Hopkins is a member of the American Chemical Society, Society for Applied Spectroscopy, Coblentz Society and SPIE. He is the author of 19 papers and proceedings and is the author of three patents.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Elena Hagemann (Applications Specialist, Metrohm USA)</p>
</blockquote>
<blockquote>
<p>Elena Hagemann is an Applications Specialist at Metrohm USA in Riverview, Florida. She earned her Master degree in Analytical and Bioanalytical Chemistry at the Applied University of Aalen, Germany, where she completed her studies with her Master thesis focused on Karl Fischer Titration, NIR Spectroscopy and Multivariate Data Analysis. Elena joined the Metrohm Group in August 2013 and started with Metrohm USA in January of 2018, focusing on Raman and NIR Spectroscopy. She performs internal as well as customer training.</p>
</blockquote>


Metrohm: Fluorescence-Free Raman at 785nm

Fluorescence-Free Raman at 785nm

We will explain how XTR works and show different use cases for both qualitative and quantitative measurements, including biodiesel and polyurethane production.

<p><strong>You might be facing challenges with respect to material identification process and its qualification.</strong></p>
<p>Now, with Agilent&#39;s trusted solution - Vaya Raman Spectometer, confidently identify pharmaceutical materials and meet the performance specifications of global pharmacopoeia.</p>
<blockquote>
<p><strong>Presenter:</strong> Rob WIlls (Molecular Spectroscopy Specialist, Agilent Technologies)</p>
</blockquote>


Agilent Technologies: Raw Material ID and Raman

Raw Material ID and Raman

Challenges with respect to material identification process and its qualification.

<p><strong>Raw materials identification (RMID) is a crucial regulatory step required before drug manufacturing. Raman spectroscopy is well-suited to making the process simpler, yet some spectrometers are challenged by light-blocking containers or struggle to reach compliance standards.</strong></p>
<p>How do you ensure that your RMID is fast and cost-effective while also meeting data integrity and compliance standards?</p>
<p>Find out in this webinar! </p>
<h5><strong>Attend this webinar to:</strong></h5>
<ul>
<li>Discover spatially offset Raman spectroscopy (SORS) – a new solution for RMID testing</li>
<li>Learn how SORS deals with common raw materials challenges, including discrimination and hard to scan materials</li>
<li>Explore how Raman spectroscopy can support your green initiative by reducing the need for consumables</li>
</ul>
<blockquote>
<p><strong>Presenter</strong>: Frederic Prulliere (Raman ID Product Manager, Agilent Technologies)</p>
</blockquote>


Technology Networks: Discover How Raw Materials Identification Testing Should Be

Discover How Raw Materials Identification Testing Should Be

How do you ensure that your RMID is fast and cost-effective while also meeting data integrity and compliance standards?

<p><strong>A real bottleneck in GMP environments can be the verification and release of incoming raw materials. Agilent's Raman systems for raw material identification can alleviate this by making measurements through opaque containers using spatially offset Raman spectroscopy (SORS).&nbsp;</strong></p><p>We will discuss Agilent's handheld Vaya and mobile RapID solutions as well as demonstrate the Vaya's capabilities.</p><blockquote><p><strong>Presener: </strong>Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)</p><p>Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</p></blockquote>

Agilent Technologies: Advance Your Raw Material ID: How Handheld Raman Can Verify Materials Through Opaque Packaging

Advance Your Raw Material ID: How Handheld Raman Can Verify Materials Through Opaque Packaging

We will discuss Agilent's handheld Vaya and mobile RapID solutions as well as demonstrate the Vaya's capabilities.

Handheld Raman: Verifying Materials Through Opaque Packaging

<p><strong>The fundamentals of fluorescence analysis will be discussed. Topics considered will be choosing the best excitation wavelength, identifying interferences like the Raman band and second order peaks from the monochromator. Concentration quenching, environmental factors, and a variety of other factors will be discussed.</strong></p><blockquote><p><strong>Presenter:&nbsp;</strong> Dean Brown (Application Scientist, Molecular Spectroscopy, Agilent Technologies, Inc.)</p><p>A graduate of the University of Maryland and West Virginia University. Started as research scientist at Johns Hopkins School of Medicine developing clinical laboratory assays. Transitions to an Applications Scientist for Uv/Vis/Nir, Fluorescence, IR and Raman spectroscopy. He has published numerous papers in the field of molecular spectroscopy and served on a number of Committees.</p></blockquote>

Agilent Technologies: Agilent Webinars On Demand

Back to Basics with Fluorescence: A Tips & Tricks Workshop

The fundamentals of fluorescence analysis will be discussed - choosing the best excitation wavelength, identifying interferences and a variety of other factors.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>This session will delve into model training, method validation, tips and tricks to improve or ensure method robustness and custom methods.</strong></p><p>Don't miss this opportunity to enhance your understanding and skills!</p><blockquote><p>First session: <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/5748"><strong>Watch on-demand here</strong></a></p></blockquote><h5><strong>In this webinar, you'll learn:</strong></h5><ul><li>How to build method training&nbsp;</li><li>Method validation</li><li>How to ensure model robustness on the Vaya?</li><li>What is a custom method on Vaya?</li></ul><p>This webinar is beneficial for all Vaya users or any pharma/biopharma customers interested to know more on through-packaging raw materials identification.</p><blockquote><p><strong>Presenter:</strong> Chris Welsby (Raman ID Product Line Manager, Agilent Technologies)</p><p>Chris is a PhD chemist, graduating from Cardiff University in 2001 having studied heterogenous catalysis, now focusing on molecular spectroscopy products intended for the pharma industry, namely Spatially Offset Raman Spectroscopy (SORS) through barrier technology, to enable verification of raw material ID through packaging in the warehouse environment. Chris has worked as part of the marketing team on Spatially Offset Raman applications since 2016, firstly as an Applications Scientist and latterly as a Marketing Product Manager of the Vaya handheld for raw material ID.</p></blockquote></div></div></div>

Agilent Technologies: Tip and Tricks – How to Best Use the Handheld Raman Vaya