This study investigates the chemical transformations of Eugenia uniflora essential oil under thermal conditions simulating gas chromatography analysis. GC–MS and NMR revealed that sesquiterpenes of the germacrene type, such as furanodiene, undergo sigmatropic rearrangement to form elemene-type compounds, including curzerene.Thermal treatment confirmed the formation of rearrangement products, indicating that bioactive properties historically attributed to curzerene may instead originate from furanodiene. The findings highlight the importance of combining complementary analytical techniques, such as NMR, to avoid misinterpretation of essential oil composition and bioactivity markers.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966">Is Curzerene Responsible for the Bioactive Properties of Eugenia uniflora? A Possible Misinterpretation of Bioactive Markers</a></h4>Vinicius Monteiro Schaffka, Raphaela Pereira Guaringue, Larissa Kozan, André Luis Kerek, Cássia Gonçalves Magalhães, Andersson Barison, and Barbara Celânia Fiorin*ACS Omega 2025, 10, 48, 59425–59434<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.5c08966">https://doi.org/10.1021/acsomega.5c08966</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div>Essential oils are a matrix of volatile and complex compounds characterized by strong odors and significant biological activity. In plants, they play a crucial role in defense against bacteria, viruses, and predators. <a href="javascript:void(0);">(1)</a> These biological properties support the extensive use of essential oils in the food, cosmetics, and pharmaceutical industries. <a href="javascript:void(0);">(1,2)</a> Eugenia uniflora L., commonly known as pitangueira, is a native Brazilian tree widely distributed across South America and is well-known for its bioactive essential oil. <a href="javascript:void(0);">(3)</a> Beyond the consumption of its fruits in natura or functional beverages, its leaves are used in infusions, decoctions, and tinctures to treat a variety of diseases, including diarrhea, stomach pain, worm infestations, fever, flu, hyperglycemia, hyperlipidemia, and hypertension. <a href="javascript:void(0);">(4,5)</a> Studies have shown that the composition of secondary metabolites in E. uniflora can vary depending on factors such as fruit color, ripening stage, <a href="javascript:void(0);">(6)</a> and seasonal influences. <a href="javascript:void(0);">(4,7−10)</a>The essential oil of E. uniflora is predominantly composed of sesquiterpenes, mainly germacrene-type, such as curzerene, germacrene B, germacrone, germacrene D, selina-1,3,7(11)-trien-8-one, and its oxide. Additionally, Elemene-type terpenes are also reported, including furanodiene, β-Elemene, and β-Elemenone. <a href="javascript:void(0);">(11−13)</a>However, those sesquiterpenes are well-documented for their thermal instability. When heat is applied, germacrene-type sesquiterpenes undergo a [3,3]-sigmatropic rearrangement, known as Cope rearrangement, converting into Elemene-type terpenes as presented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig1">Figure 1</a>. <a href="javascript:void(0);">(14−16)</a> This reaction is widely utilized as a synthetic pathway to produce Elemenes from germacrene precursors. <a href="javascript:void(0);">(17,18)</a><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_1_3_3_Sigmatropic_rearrangement_of_Germacrene_type_into_Elemene_type_250708e73c.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 1. [3,3]-Sigmatropic rearrangement of Germacrene-type into Elemene-type." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_1_3_3_Sigmatropic_rearrangement_of_Germacrene_type_into_Elemene_type_250708e73c.jpg 245w," sizes="100vw" width="3000" height="984">Gas chromatography (GC) is the main technique for characterizing essential oils, often coupled with mass spectrometry (GC-MS) and the retention index determination is used in the identification of the compounds through comparison with databases. <a href="javascript:void(0);">(21)</a> During GC analysis, essential oils are subjected to multiple heating stages. Initially, the sample is exposed to a high inlet temperature, typically around 240 °C, to ensure complete volatilization. It then enters the oven, where the temperature follows a programmed ramp starting at 60 °C, increasing at 3 °C/min, and reaching up to 240 °C. Finally, the sample encounters the elevated temperatures of the mass spectrometry (MS) detector’s source (250 °C) and interface (280 °C). Even in isocratic methods, where the oven temperature remains constant throughout the analysis, the inlet, source, and interface temperatures are maintained at high levels. Although the sample passes through these stages briefly, the elevated temperatures can induce Cope rearrangements in the essential oil, leading to possible changes in its composition. <a href="javascript:void(0);">(22,23)</a>The accurate characterization of the chemical composition of essential oils is crucial for any study employing them as a biological or analytical matrix, as it supports the interpretation of bioactivity, chemotaxonomic classification, and quality control of samples with economic value added. There are many studies attributing relevant biological activities, classifying specimens, and identifying phenotypic and genetic variations based on the chemical composition of the essential oil of E. uniflora L., which is usually characterized by GC-MS. <a href="javascript:void(0);">(8,9,11,24)</a> As an example of the study carried out by de Jesus, found acute anti-inflammatory effect of a rich in curzerene essential oil. <a href="javascript:void(0);">(5)</a> Similarly, Pascoal, reported distinct essential oil composition patterns among E. uniflora varieties─yellow, red, and purple fruits─primarily driven by variations in β-Elemene and germacrene D content. <a href="javascript:void(0);">(7)</a> These findings highlight metabolic changes during fruit ripening and underscore the influence of genetic diversity among E. uniflora varieties. Such exhibitions are unsatisfactory, because they sustain all their evidence in the characterization of their essential oil only by GC-MS analysis, without considering the Cope rearrangements, a fact that may change the outcome of their research. In this context Nuclear Magnetic Resonance (NMR) spectroscopy is a good alternative for essential oil analysis, since is a robust not destructive technique and able to detect and quantify simultaneously a wide range of metabolites that exceed the concentration of about 5–10 μM. <a href="javascript:void(0);">(25)</a>In order to highlight analytical concerns about the uses of only GC-MS data to access chemical relevance of E. uniflora essential oil studies, Nuclear Magnetic Resonance (NMR) spectroscopy was employed to evaluate the chemical composition of the essential oil of Eugenia uniflora L. before and after an induced thermal treatment, compared to GC-MS data. The findings provide insights into thermolabile compounds and highlight potential misidentifications of the bioactive properties associated with the sesquiterpenes of E. uniflora present in literature.<h4>2. Results and Discussion</h4><h5>2.1. Characterization of the Essential Oil</h5>Approximately 60 compounds, mostly sesquiterpenes (Germacrene B) and oxygenated sesquiterpenes (Germacrone), were visualized in the essential oil, with the 9 compounds having a relative area above 2%, as shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#tbl1">Table 1</a>. The identified and most prevalent compounds align with those reported in the literature for E. uniflora. <a href="javascript:void(0);">(4,11,12)</a> It is possible to highlight the significant proportion of Germacrene-type sesquiterpenes compared to Elemene-type, except Curzerene, which was found in high concentration.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Table_1_Main_Components_Identified_in_the_GC_MS_Analysis_of_the_Essential_Oil_from_Leaves_of_E_uniflora_9d59d96bef.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Table 1. Main Components Identified in the GC–MS Analysis of the Essential Oil from Leaves of E. uniflora." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Table_1_Main_Components_Identified_in_the_GC_MS_Analysis_of_the_Essential_Oil_from_Leaves_of_E_uniflora_9d59d96bef.jpg 218w," sizes="100vw" width="1076" height="771">The variation in the composition of the essential oil from E. uniflora leaves was described in literature, <a href="javascript:void(0);">(9)</a> who classified the specimens into three groups: Group I, containing yellow, dark red, and purple fruits, which showed high concentrations of germacrene B, germacrone, and atractylone; Group II, consisting of light red fruit samples, with high concentrations of curzerene, germacrene D, and germacrene A; and Group III, containing orange-red fruits, with a high content of selina-1,3,7(11)-trien-8-one and oxyselina-1,3,7(11)-trien-8-one. According to this classification, this essential oil fits into Group II.Chemical transformations of many components found in the E. uniflora leaves essential oil result from the heating conditions typically applied during GC–MS analysis, which occur through Cope Rearrangement. <a href="javascript:void(0);">(12,19)</a> Cope rearrangement is a stereospecific [3,3]-sigmatropic rearrangement that proceeds via the most stable chairlike transition state of 1,5-cyclodecadiene, from germacrene-type, to give 1,2-divinylcyclohexane, known as Elemene-type. <a href="javascript:void(0);">(14)</a> Some of the Cope rearrangements found in the E. uniflora essential oil are represented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig2">Figure 2</a>. <a href="javascript:void(0);">(11,22,23,26)</a><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_2_Cope_Rearrangements_of_sesquiterpenes_7fe9282d75.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 2. Cope Rearrangements of sesquiterpenes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_2_Cope_Rearrangements_of_sesquiterpenes_7fe9282d75.jpg 150w," sizes="100vw" width="1500" height="1566">The same essential oil was analyzed using NMR spectroscopy to identify its components. Although NMR identification is considerably more labor-intensive than GC-MS analysis, it offers the advantage of being a cold and nondestructive technique. Since the sample does not undergo heating during the process, its stability is preserved throughout the analysis. The conformational differences observed in germacrene-type compounds further increase the spectrum’s complexity, leading to signal broadening in the 1H NMR spectra. <a href="javascript:void(0);">(27)</a>A substantial number of signals were observed in the 1H spectrum, with the majority concentrated between 0.5 and 3.0 ppm, a region characteristic of terpene compounds. The region between 4.5 and 6.0 ppm also exhibited numerous signals attributed to vinylic protons, observed in sesquiterpenes (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig3">Figure 3</a>).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_3_1_H_NMR_400_13_M_Hz_CD_Cl3_spectra_of_the_raw_essential_oil_a46e04574e.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 3. 1H NMR (400.13 MHz, CDCl3) spectra of the raw essential oil." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_3_1_H_NMR_400_13_M_Hz_CD_Cl3_spectra_of_the_raw_essential_oil_a46e04574e.jpg 245w," sizes="100vw" width="4175" height="2376"><h4>3. Conclusions</h4>The investigation of the composition of E. uniflora essential oil by NMR and the thermal treatment performed proved that curzerene may come from furanodiene due to the transformations that occur in the matrix during gas chromatographic analysis. These findings have significant implications for the investigation into the chemical composition of E. uniflora essential oil and the evaluation of the biological properties of its sesquiterpenes. Since each technique has advantages and disadvantages, combining GC-MS analysis with a cold technique, such as NMR is important to guarantee the proper identification of the compound genuinely responsible for any property assigned to E. uniflora essential oil.<h4>4. Materials and Methods</h4><h5>4.2. GC-MS Analysis</h5>The gas chromatographic analyses of the essential oils (10 mg mL–1) were performed using a gas chromatography–mass spectrometry system (<a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=QP2020">Shimadzu GCMS-QP2020 Gas Chromatograph</a>). The analyses were conducted on an RTx-5MS capillary column (30 m × 0.25 mm internal diameter × 0.25 μm film thickness), and the analytical conditions were as follows: split ratio of 1/10, injector temperature at 250 °C, ion source at 250 °C, and interface at 280 °C. The temperature program of the oven was set at 60 °C for 5 min, followed by a temperature ramp of 3 °C/min until reaching the final temperature of 240 °C. The components were identified based on the relative retention index, calculated for each constituent by injecting a series of n-alkane standards (C8–C20) under the same sample conditions and comparing them with tabulated values as well as by comparing the obtained mass spectra with the mass spectra database and literature comparisons. <a href="javascript:void(0);">(21)</a><h5>4.4. NMR Analysis</h5>All NMR spectra were acquired at 298 K on a Bruker AVANCE III spectrometer operating at 9.4 T, observing 1H at 400.13 MHz and 13C at 100.13 MHz, equipped with a broadband probe. The essential oil was solubilized in CDCl3 with 0.05% of tetramethylsilane (TMS) used as an internal reference. The 1H NMR spectra were recorded utilizing 65k time-domain data points across a spectral width of 20.00 ppm. This setup provided a digital resolution of 0.24 Hz. The data were obtained using a single 30° excitation pulse, with a relaxation delay of 1.0 s, and an average of 128 scans. The 13C NMR spectra was carried out using the zgpg30 pulse sequence utilizing 64k time-domain data points across a spectral width of 238 ppm, with an acquisition time of 1.3631 s, providing a digital resolution of 0.7434 Hz and a relaxation delay of 2.0s and an average of 2k scans.The HSQC and HMBC experiments were conducted using standard pulse sequences from the Bruker library, with modifications to the number of acquired points to improve resolution, 2048 in F2 and 512 in F1.</div>

Is Curzerene Responsible for the Bioactive Properties of Eugenia uniflora? A Possible Misinterpretation of Bioactive Markers

Quantitative Assessment of Chemical Modifications of Macromolecules through Counterion Measurement

In the week from March 30, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Bruker: MALDI Imaging Becomes IR-resistible: Going Multimodal for Advanced Biomedical Insights</h5><ul><li>Mo, 30.3.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/5971">Registration</a></li></ul>Explore advanced multimodal imaging with MALDI, QCL-based IR, and fluorescence microscopy to achieve ROI-focused molecular insights in complex tissues.<h5>2. LECO: Moisture and LOI Determination in Cement, Fly Ash, Gypsum, and Lime</h5><ul><li>Tu, 31.3.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/5972">Registration</a></li></ul>Learn how TGA supports accurate moisture and LOI determination in cement, fly ash, gypsum, and lime, ensuring quality control and compliance with ASTM methods.<h5>3. Thermo Fisher Scientific: FTIR microscopy: Practical guidance for effective sample analysis</h5><ul><li>Tu, 31.3.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6088">Registration</a></li></ul>Join this webinar to gain practical guidance on FTIR microscopy workflows, including measurement modes, sample preparation, parameter optimization and spectral interpretation.<h5>4. Bruker: Understanding Polymer Reactions and Degradation with FT-IR and EPR Spectroscopy</h5><ul><li>Tu, 31.3.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/5996">Registration</a></li></ul>Discover how FT-IR and EPR spectroscopy reveal polymer curing kinetics and radical-driven mechanisms—enabling real-time monitoring, degree of cure quantification, and stability studies.<h5>5. Agilent Technologies: Fueling Accuracy: ICP-OES Analysis for Organic Biofuel and Renewable Fuel Feedstocks</h5><ul><li>Tu, 31.3.2026 20:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6043">Registration</a></li></ul>This webinar provides a practical overview of setting up ICP-OES instrumentation for organic sample analysis.<h5>6. C&amp;EN: Overcoming Raman Microscopy Challenges in Microplastics and Life Sciences</h5><ul><li>We, 1.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6016">Registration</a></li></ul>Learn how O-PTIR spectroscopy overcomes fluorescence limitations in Raman microscopy. Discover simultaneous sub-micron IR and Raman analysis for microplastics, biological samples, and organic materials.<h5>7. SPECTRO Analytical Instruments: AI-Driven Semiquantitative ICP-OES Analysis: Introducing SPECTRO AI QUANT</h5><ul><li>Th, 2.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6090">Registration</a></li></ul>This live webinar introduces an AI-driven approach that streamlines semiquantitative analysis across a wide range of sample types.<h5>8. Anton Paar: Extensional Rheology of Polymers: Air Bearing Rheometer Applications</h5><ul><li>Th, 2.4.2026 18:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/5800">Registration</a></li></ul>Learn why extensional rheology is essential for polymer processing. See real measurements, core concepts, and practical examples, and explore SER and UXF solutions for elongational flow testing.<h5>9. Agilent Technologies: Heavy Metal Elements in Food Spices: A Critical Analysis Using ICP-MS and ICP-OES Techniques</h5><ul><li>Th, 2.4.2026 19:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6044">Registration</a></li></ul>This session discusses techniques for determination of heavy metals in food by ICP-OES and ICP-MS.

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TBS... Elemental Analysis of Lithium-ion Battery Samples using ICP-OES and ICP-MS
<blockquote>
Presenter: L. Craig Jones (ICP-MS Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Craig has been with Agilent for over 15 years as an ICP-MS applications scientist. He has been involved with multiple type of applications for ICP-MS, including environmental, pharmaceutical, semiconductor, geologic, and clinical analyses, to name a few. Previous to Agilent he worked in an environmental lab performing analysis and supervising both the inorganic and organic sections of the laboratory. In his spare time, Craig enjoys volunteering at the local marine science center, mountain biking, hiking, and relaxing at the beach. Craig obtained a Bachelor of Science degree in chemistry from Fort Lewis College in Durango, CO.
</blockquote>
<blockquote>
Presenter: Sima Singha, PhD (ICP-OES Application Scientist, Atomic Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Sima has over fifteen years of hands on experience as an applications scientist in the field of atomic spectroscopy. Before joining Agilent in 2017, managed an agricultural chemistry laboratory for the analysis of water, soil, and plant tissue digests using ICP-OES for various EPA methods. She received a Ph.D. degree in Chemistry from the University of Illinois at Chicago with numerous peer-reviewed publications.
</blockquote>

Agilent Technologies: Elemental Analysis of Lithium-ion Battery Samples using ICP-OES and ICP-MS

Elemental Analysis of Lithium-ion Battery Samples using ICP-OES and ICP-MS

Lithium-ion Batteries Analysis using ICP-OES and ICP-MS

TBS... Agilent M Lens for ICP-MS and the Semiconductor Industry
<blockquote>
Presenter: Bert Woods (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
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Joined the Agilent ICP-MS team in 2004, with previous employment in the semiconductor industry with Dominion Semiconductor (IBM/Toshiba) and Micron. Bert is a 1997 Chemistry graduate of Radford University in Virginia and an avid Washington DC Sports fan.
</blockquote>

Agilent technologies: Agilent M Lens for ICP-MS and the Semiconductor Industry

Agilent M Lens for ICP-MS and the Semiconductor Industry

This webinar explores the limits of flow cytometry technology and outline how the CytoFLEX nano flow cytometer is designed to help address these limitations in a robust and consistent system able to standardize results between labs. Our expert speaker, Alfonso Blanco of University College Dublin, will discuss the advantages of using fluorescence combined with multiple scatters for further characterization of nanoparticles and extracellular vesicles. In addition, he will present some preliminary results research groups have obtained with the CytoFLEX instrument.
Key learning objectives
<ul>
<li>Discover the current limits for flow cytometry</li>
<li>Gain an introduction to the CytoFLEX nano flow cytometer</li>
<li>Explore samples run</li>
</ul>
Who should attend?
Core lab managers, principal investigators, research scientists, core facility managers, data scientists, analysts, professors, instructors, researchers, postdoctoral research fellows, laboratory assistants, laboratory coordinators, laboratory specialists, group leaders
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Alfonso Blanco (Director of Flow, University College Dublin)
</blockquote>
<blockquote>
Moderator: Charlie Carter (Associate Editor, SelectScience)
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SelectScience: Advancing EV research – First Experiences with the CytoFLEX Nano Flow Cytometer

Advancing EV research – First Experiences with the CytoFLEX Nano Flow Cytometer

The advantages of using fluorescence combined with multiple scatters for further characterization of nanoparticles and extracellular vesicles.

First Experiences with the CytoFLEX Nano Flow Cytometer

Digitalizing laboratory sample workflows is crucial yet challenging due to complex data types, resource constraints, and the need for integration, automation, training, and more.
But with CaliberLIMS, these challenges become opportunities. CaliberLIMS is a robust, scalable, and reliable platform designed to meet the demands of modern laboratories aiming for Pharma 4.0.
With the upgraded version, CaliberLIMS 4.0 enables users to effortlessly adopt a futuristic workflow that integrates instruments, workflows, and data across the entire laboratory. Packed with powerful features, this advanced software can enhance compliance, integrity, and productivity, making your laboratory tomorrow-ready.
Register now to secure your spot!
Learning Objectives:
<ul>
<li>Learn how LIMS application can accelerate laboratory efficiency </li>
<li>Explore how digitalization can be made easier with scalable solutions </li>
<li>Understand how to achieve seamless integrations with CaliberLIMS for automated workflows</li>
<li>Discover the latest advancements in Caliber 4.0</li>
</ul>
<blockquote>
Presenter: Kanimozhi Sivarajan (Informatics Manager, Shimadzu (Asia Pacific) Pte Ltd)
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<blockquote>
Dr. Kani is having 10 years of experince in Laboratory Management and Informatics Systems. She is serving as Informatics Manager at Shimadzu Asia Pacific, Singapore, providing Lab Informatics Solutions such as LabSolutions CS, LIMS and other QMS applications. She is supporting customers from various segments on implementing laboratory automation and responsible for providing consultation on streamlining Lab Workflows, Regulatory Compliance requirements for Electronic Data Management and Lab Practices/Analysis. 
</blockquote>

Shimadzu: Elevating Pharma 4.0: Harnessing CaliberLIMS For Digital Excellence

Elevating Pharma 4.0: Harnessing CaliberLIMS For Digital Excellence

CaliberLIMS is a robust, scalable, and reliable platform designed to meet the demands of modern laboratories aiming for Pharma 4.0.

 Pharma 4.0: CaliberLIMS For Digital Excellence

<h4>Unraveling Epigenetic Changes in Cancer Cells</h4>
During this webinar, guest speaker Dr. Babak Faryabi, University of Pennsylvania, discusses utilizing Oligopaint and super-resolution microscopy to investigate the role of transcription hubs in altering genome folding.
Attendees can expect to learn about the power of super-resolution microscopy and chromatin tracing in understanding the three-dimensional structure of the genome in single cells.
The importance of epigenetic changes in cancer pathogenesis has become increasingly clear, yet the underlying mechanisms remain obscure. Our research focuses on identifying epigenetic changes that can render cancer cells addicted to specific transcriptional regulators. I will present evidence that oncogenic subversion of transcription factors leads to disruption of chromosome topology and instruct enhancer elements to aberrantly interact and activate intact key proto-oncogenes. These aberrant epigenetic states can drive cancer cell growth and proliferation in the absence of genetic mutations and may also confer drug resistance. I will also discuss our efforts to develop single-cell molecular tools and experimental models to characterize epigenetic mechanisms of oncogenesis and attendant therapeutic opportunities.
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Presenter: Babak Faryabi, Ph.D. (Associate Professor of Pathology and Cancer Biology, University of Pennsylvania Perelman School of Medicine)
</blockquote>
<blockquote>
Dr. Faryabi has a distinguished history of research and accomplishments in cancer epigenomics. His work has significantly advanced our understanding of how transcription factors alter cancer genome folding. By conducting the first genome-wide and single-cell resolution measurement of genome looping in triple-negative breast cancer (TNBC) and mantle cell lymphoma (MCL), he discovered that Notch can regulate gene expression by changing genome folding and bringing together distant enhancer and promoter elements on the linear genome. This identified a novel mechanism by which oncogenic Notch alters transcription. Furthermore, he uncovered that Notch-dependent DNA loops bring multiple genomic elements into highly interacting spatial hubs of enhancers and promoters. He is among the first to elucidate the contribution of genome topology organization to cancer therapy resistance. His work revealed the role of genome folding reorganization as a novel epigenetic adaptation mechanism, conferring resistance to Notch inhibitors in T-cell leukemia and to BTK inhibitors in B cell lymphoma. To achieve his central scientific objective, he also innovates machine learning tools and software to better dissect the complexity and heterogeneity of genome structures and regulation in cancer.
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As the faculty lead at the Center for Personalized Diagnostics at the Hospital of the University of Pennsylvania, his clinical expertise lies in developing genomic assays for diagnostic and predictive markers in cancer.
</blockquote>

Bruker: Notch-Mutated Cancer Genomes in 3D

Notch-Mutated Cancer Genomes in 3D

Find out what super-resolution microscopy can reveal about epigenetic changes in cancer pathogenesis.

In an effort to build smarter factories, many industry leaders have started moving the point-of-analysis from the laboratory to process.
Clearly actionable data generated by Process Analytical Technology (PAT) makes this paradigm shift possible. PAT not only enhances Predictive Maintenance (PdM) by detecting issues early and minimizing downtime, it also supports Statistical Process Control (SPC) through real-time monitoring and optimization, and improves Quality by Design (QbD) with better process understanding and risk management.
In this webinar, Chad Shade, Global PAT Team Leader, will share how Albemarle Corporation approached the implementation of Spectroscopy PAT to obtain smarter process operations and what you can do to help drive a mindset change in your business. Elena Hagemann, Product Manager for Process Spectroscopy, will introduce the newest spectroscopy technologies for real-time, non-destructive analysis that further support sustainability initiatives.
Key Learning Objectives:
<ul>
<li>Staying Competitive: Attendees will gain knowledge that can give them an edge over competitors who may not be leveraging these advanced technologies.
</li>
<li>Enhanced Process Understanding: Attendees will learn how Spectroscopy PAT can be applied to optimize processes, ensuring better quality and efficiency.
</li>
<li>Managing Expectations: Attendees will discover practical tips on fostering a culture shift within their organization to embrace advanced Spectroscopy PAT solutions.
</li>
<li>Actionable Strategies: Attendees will learn the steps and considerations to successfully implement Spectroscopy PAT for enhanced process operations.
</li>
<li>Innovation: Attendees will explore the latest spectroscopy technologies that enable real-time, non-destructive analysis and support sustainability initiatives.
</li>
</ul>
Who Should Attend:
<ul>
<li>Production manager / Operational manager/ Plant manager
</li>
<li>Process engineers
</li>
<li>Product development scientists
</li>
<li>Quality control specialists
</li>
</ul>
<blockquote>
Presenter: Chad Shade (Global Process Analytical Team Leader, Albemarle Corporation)
</blockquote>
<blockquote>
Preenter: Elena Hagemann (Spectroscopy Product Manager, Process Analytics, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Kelly McSweeney (Contributing Editor, C&amp;EN Media Group)
</blockquote>

CEN: Smart and Actionable: Spectroscopy PAT-Supported Process Operations

Smart and Actionable: Spectroscopy PAT-Supported Process Operations

How Albemarle Corporation approached the implementation of Spectroscopy PAT to obtain smarter process operations.

Smart and Actionable: Spectroscopy PAT-Supported Process

Adeno-associated vectors (AAVs) are transforming therapeutics – from gene therapy to vaccines.
A major challenge when developing AAV-based therapeutics is ensuring that empty AAVs that lack therapeutic DNA are removed, increasing the efficacy of the product.
But the empty AAVs are chemically identical to their full counterparts, making this process complicated. Enter mass photometry, a new bioanalytical technique that can efficiently characterize AAVs and distinguish between full and empty AAVs.
This webinar explores how mass photometry can circumvent challenges associated with AAVs, helping to accelerate therapeutic development and manufacturing.
Attend this webinar to: 
<ul>
<li>Discover how mass photometry is currently being used in industry</li>
<li>Learn how mass photometry can accurately measure empty/full AAV capsid ratios</li>
<li>Explore how to integrate mass photometry in a GMP-regulated environment</li>
</ul>
Will I receive a certificate of attendance?
Yes, when attending the webinar, you will be able to download a certificate of attendance from the literature section.
Will the webinar be available to watch On-Demand?
Yes, after the live date the recording will be available to watch. Once registered, you will receive an email notifying you when the recording is available along with your on-demand link.
<ul>
<li>Presenter: Dr. Matt Ranaghan (Senior Applications Scientist, Refeyn)</li>
</ul>

Technology Networks: Enhancing the Development of AAV-Based Therapeutics With Mass Photometry

Enhancing the Development of AAV-Based Therapeutics With Mass Photometry

Mass photometry, a new bioanalytical technique that can efficiently characterize AAVs and distinguish between full and empty AAVs.

Development of AAV-Based Therapeutics With Mass Photometry

In this webinar, Caroline Roe, Managing Director, Cancer and Immunology Core, Vanderbilt University, will give a broad overview of available high-dimensional flow cytometry technologies and reagents, how they work, and an experience-informed breakdown of the strengths and limitations of each technique. She will review considerations for choosing the appropriate high-dimensional flow cytometry technology for a project. In addition, she will provide guidance for those who have experience in one technology and are transitioning to another.
Key topics discussed in this webinar will include:
<ul>
<li>An overview of high-dimensional flow cytometry technologies and reagents</li>
<li>Considerations for choosing the appropriate high-dimensional flow cytometry technology for a project</li>
<li>Guidance for those who are transitioning to a new flow cytometry technology</li>
</ul>
Who should attend?
<ul>
<li>Flow cytometry novices who wish to learn more about high-dimensional flow cytometry</li>
<li>Experienced flow cytometrists who are new to high-dimensional flow cytometry</li>
<li>Anyone seeking to have a deeper understanding of high-dimensional flow technologies</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Caroline E. Roe, MLI (Managing Director, Cancer and Immunology Core, Vanderbilt University)
</blockquote>
<blockquote>
Caroline Roe received her BS.c. in Genetics, Cell Biology, and Development from the University of Minnesota-Twin Cities, and Master of Laboratory Investigation from Vanderbilt University in Nashville, Tennessee, where she has managed the Mass Cytometry Center of Excellence since its inception in 2017. As director of the Cancer and Immunology Core, her focus is on increasing both the standardization and accessibility of cutting-edge cytometry techniques for application across a broad range of basic and translational research projects.
</blockquote>
<blockquote>
Moderator: Charlie Carter (Associate Editor, SelectScience)
</blockquote>

SelectScience: Improving Results Through Better Understanding: A Primer On High-Dimensional Flow Cytometry Technologies And Reagents

Improving Results Through Better Understanding: A Primer On High-Dimensional Flow Cytometry Technologies And Reagents

A broad overview of available high-dimensional flow cytometry technologies and reagents.

A Primer On High-Dimensional Flow Cytometry Technologies

High matrix samples, such as those containing high dissolved solids, can often be challenging potentially resulting in poor data quality and instrument performance, leading to excess maintenance.
In this webinar we will discuss the techniques available to deal with these samples.
<blockquote>
Presenter: David Fishwick (Applications Specialist, TEA, Thermo Fisher Scientific)
</blockquote>
<blockquote>
David joined Thermo in 2022 as an Applications Specialist for the Trace Elemental Analysis product range in the UK and Ireland. Prior to joining Thermo, David has gained experience of trace elemental analysis and chromatography in industry, working in GMP, GLP and UKAS accrediated R&amp;D and pharmaceutical laboratories. David holds a BSc in Chemistry from Bangor University.
</blockquote>
<blockquote>
Presenter: Matthew Gregory (Applications Specialist, TEA, Thermo Fisher Scientific)
</blockquote>
<blockquote>
After graduating from the University of Surrey with an MChem in Chemistry, Matthew has gained 20 years’ experience of trace elemental analysis in industry, working in environmental and precious metal research laboratories. In 2018, Matthew joined Thermo Fisher Scientific as an Applications Specialist for the Trace Elemental Analysis product range in the UK and Ireland.
</blockquote>

Thermo Scientific: Dealing with the Complexity of High Matrix Samples on ICP-OES and ICP-MS

Dealing with the Complexity of High Matrix Samples on ICP-OES and ICP-MS

Techniques available to deal with these samples.

Complexity of High Matrix Samples ICP-OES amd ICP-MS

Rice fortification is a cost-effective solution for addressing nutritional challenges by adding Iron, Folic acid, and Vitamin B12. Our innovative Equipment offers robust and reliable performance with high sensitivity and throughput, able to test for challenging samples and analytes.
This webinar offers valuable insights into the latest advancements in analytical testing for fortified rice, catering to food manufacturers, regulatory agencies, and research institutions.

PerkinElmer: Fortify Rice in a compliant way

Fortify Rice in a compliant way

Valuable insights into the latest advancements in analytical testing for fortified rice.

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