Utilizing Imaging Flow Cytometry To Empower Studies Of Erythropoiesis
Two powerful single cell analytical methods have propelled the field of cell biology forward. Microscopy established the concept of a single cell over 300 years ago and has revealed thousands of features that are currently used to differentiate cells and delineate the processes of maturation and disease. Conventional flow cytometry is limited in the number of morphological and spatial features measured, but it is highly quantifiable both in the number of cells analyzed and the accuracy of measurements. Imaging flow cytometry (IFC) combines the strengths of both systems. Importantly, while conventional flow cytometry analysis has been particularly vital in the field of immunology, there have been challenges in applying it to cells that are defined by morphology and for which there are few distinguishing flow cytometry antibodies available. Thus, for these reasons, we turned to IFC in our studies of erythropoiesis.
Key topics discussed in this webinar will include:
- Delineating stages of erythropoiesis from early progenitor to terminal maturation with flow cytometry and IFC
- Issues and potentials for examining microenvironmental interactions with erythroid cells in bone marrow, particularly with macrophages
- Improving flow cytometry analysis by using IFC to remove artifacts
- Synergizing single cell assays by integrating morphology and fluorescence analysis on the same cell
- Combining advances in computer analysis with the inherent analytical power of human visual processing to manage the high dimensionality of IFC data
Who should attend:
- Researchers using IFC who wish to enhance and expand their assay repertoire
- Scientists considering adding IFC to their single cell analysis tool kit
- Scientists studying erythroid differentiation and macrophage interactions
- Anyone interested in imaging and flow cytometry applications
Presenter: Dr. Kathleen McGrath (Research Associate Professor, University of Rochester Medical Center)
