UV-VIS Spectrophotometer UV-2600i UV-2700i

Importance of the Topic

Ultraviolet-visible spectroscopy is fundamental in analytical chemistry because it enables rapid, non-destructive analysis of a broad range of samples. Modern UV-Vis systems with extended wavelength range and low stray light allow highly precise absorbance and reflectance measurements across fields such as electronics, environment, pharmaceuticals, cosmetics, textiles, and materials science.

Study Objectives and Overview

This application note presents the Shimadzu UV-2600i single monochromator and UV-2700i double monochromator spectrophotometers. It highlights their extended spectral range, ultra-low stray light performance, modular accessories, and advanced software for data acquisition, processing, and compliance with regulatory frameworks. The goal is to demonstrate versatility, precision, and integration capabilities for routine and demanding analyses.

Methodology and Instrumentation

• UV-2600i single monochromator system covers 185 to 1400 nm with optional ISR-2600Plus integrating sphere for diffuse reflectance and near-infrared transmittance
• UV-2700i double monochromator achieves stray light suppression down to 0.000001 percent transmittance (8 Abs), enabling high-concentration absorbance and polarization film measurements
• Accessories include integrating spheres, multipurpose compartments, polarized and specular reflectance attachments, film holders, temperature-controlled cells, and autosamplers
• Advanced LabSolutions software provides spectral evaluation, quantitation, kinetic studies, color, film thickness, UPF, Tm analysis, automated sequences, and FDA 21 CFR Part 11 compliance

Main Results and Discussion

• Band gap analysis of solar cell materials using diffuse reflectance and Tauc plot calculation yielded clear differences between CuIn0.5Ga0.5Se2 and CuInSe2
• Film thickness determination by interference fringe analysis delivered accurate measurements for polyvinylidene chloride, nylon, and polypropylene films
• Thermal melting transition Tm of oligonucleotide therapeutics was characterized across concentration ranges, demonstrating precise temperature control and absorbance monitoring
• UV protection factor of textiles and umbrellas, turbidity and trace metal quantitation in environmental water samples, and vitamin B2 calibration exhibited strong linearity and repeatability
• Variable angle measurements of dielectric films confirmed angle-dependent shifts in transmission and reflection spectra
• Autosampler integration enabled unattended analysis of up to 360 samples, reducing operator workload
• Data management features ensured integrity, audit trails, electronic signatures, and database centralization aligned with ER ES and FDA 21 CFR Part 11 requirements

Benefits and Practical Applications

• Scalable design supports analyses from UV to near infrared, meeting diverse research and QA QC demands
• Ultra-low stray light performance permits direct measurement of highly absorbing samples without dilution
• Modular attachments facilitate reflectance, transmittance, thin film, and polarization studies
• Automated sequences and software macros enhance reproducibility and throughput
• Comprehensive compliance tools streamline regulatory documentation and secure data handling

Future Trends and Applications

Continued integration of IoT, machine learning, and self diagnostic functions will further automate laboratory workflows and predictive maintenance. Expansion of cloud based data analytics and AI driven interpretation promises faster method development and real time quality control across multidisciplinary environments.

Conclusion

The Shimadzu UV-2600i and UV-2700i spectrophotometers deliver high precision, extended range, and robust data integrity for a wide variety of analytical tasks. Their flexible accessory platform and advanced software suite enable efficient, compliant operation in research, manufacturing, and regulatory settings.

Used Instrumentation

• Shimadzu UV-2600i Single Monochromator UV-Vis Spectrophotometer
• Shimadzu UV-2700i Double Monochromator UV-Vis Spectrophotometer
• ISR-2600Plus Integrating Sphere Attachment
• LabSolutions UV-Vis Software Suite
• TMSPC-8i Thermoelectric Multicell Holder
• ASX-560/280 Autosampler Systems