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Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Agilent Cary Eclipse

 

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Minimizing photobleaching of Blue Fluorescent Protein (BFP) using the Agilent Cary Eclipse fluorescence spectrophotometer Application Note Author Introduction Paul Gavin*, Mark Prescott*, Ph.D, Daren J. Fyfe, Ph.D and Jeffrey J. Comerford**, Ph.D The term photobleaching refers to loss of fluorescence…
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Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Agilent Cary Eclipse Application Note Author Paul Gavin# and Mark Prescott#, Ph.D Daren J. Fyfe, Ph.D* #Department of Biochemistry and…
Key words
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Agilent Cary Eclipse fluorescence spectrophotometer
2019|Agilent Technologies|Brochures and specifications
Sensitive. Accurate. Flexible. Agilent Cary Eclipse fluorescence spectrophotometer Agilent Cary Eclipse Fluorescence Spectrophotometer Agilent is your premier resource and partner for molecular spectroscopy. The world-renowned Cary product line, encompasses FTIR, UV-Vis-NIR, and fluorescence instruments, offering you a comprehensive range of…
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Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package Application Note Author Katherine Lighton, Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia. Mark J. Fiandaca, Boston Probes, Bedford, Massachusetts 01730,…
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