Konference CEEPC/IPM/CMSC 2022 - Sborník abstraktů

Significance of the Topic

Proteomics and mass spectrometry have become indispensable tools in modern biomedical, clinical, and industrial analytics. The 16th Central and Eastern European Proteomic Conference, held in conjunction with the 10th Czech Mass Spectrometry Conference, brought together experts from across Europe to showcase cutting-edge developments in protein and metabolite analysis. This gathering highlighted the critical role of advanced mass spectrometric instrumentation, multi-omics approaches, and bioinformatics in addressing pressing challenges in disease biomarker discovery, drug development, environmental monitoring, and precision medicine.

Goals and Overview of the Conference

The conference was designed to:

• Present advances in proteomic and metabolomic technologies, including high-resolution instruments and novel sample preparation workflows.
• Discuss applications of mass spectrometry in clinical diagnostics, biomedicine, and industrial analytics.
• Foster interdisciplinary collaboration, with emphasis on translating proteomic discoveries into practical solutions.
• Support young researchers through poster sessions, short-talk awards, and dedicated networking events.

Methodology and Instrumentation

Key methodological themes and technologies included:

• High-throughput data-independent acquisition (DIA) methods such as dia-PASEF with TIMS-MS.
• Single-cell proteomics workflows employing sub-nanoliter sample handling and TMT multiplexing.
• Top-down and middle-up approaches for intact protein and antibody characterization using cyclic ion mobility and native MS.
• Advanced separation techniques: microflow LC-MS/MS, capillary electrophoresis-MS, and two-dimensional ion mobility mass spectrometry.
• Non-canonical omics: glycoproteomics, lipidomics, metabolomics, and nucleic acid adduct analysis.
• Innovative bioinformatic platforms for spectral library development, retention time prediction, and network analysis.

Main Results and Discussion

Highlights from plenary lectures and sessions:

• Single-cell proteomics demonstrated feasibility of mapping >1,500 proteins per cell and distinguishing cell types in organoid models.
• FT-ICR-MS metabolomics revealed deep coverage of small molecules from diverse biological and environmental samples.
• Native MS coupled with X-ray free-electron laser sample delivery showcased single-particle imaging possibilities.
• Novel ion mobility-resolved phosphoproteomics increased depth and throughput of dynamic signaling studies.
• Biomarker discovery in clinical contexts: tear fluid analysis for ocular and systemic diseases, blood plasma lipidomics in atherosclerosis and heart failure, and cerebrospinal fluid profiling in neurodegeneration.
• Applications in plant science and biotechnology: proteomic mapping of pathogen resistance, liposome-assisted lipopeptide analysis, and forensic proteomics for species identification.

Benefits and Practical Applications

The conference outcomes advance practical applications:

• Improved diagnostic assays based on high-sensitivity immunoassays and mass spectrometric assays for proteins, metabolites, and lipids.
• Standardized workflows for therapeutic drug monitoring of monoclonal antibodies, emerging peptide therapeutics, and small-molecule drugs.
• Novel biomarkers and mechanistic insights in cardiovascular, neurological, and infectious diseases.
• Enhanced quality control methods in food safety, cultural heritage pigment analysis, and environmental monitoring.
• Accelerated development of precision medicine strategies through multi-omics integration.

Future Trends and Possibilities

Emerging directions discussed at the conference:

• Integration of single-cell proteomics with spatial and imaging technologies for tissue atlases.
• Exploiting machine-learning and artificial intelligence to streamline data analysis, spectral library generation, and predictive models.
• Expansion of top-down MS technologies to handle megadalton protein assemblies and membrane proteins.
• Wider adoption of microflow and capillary electrophoresis-MS for clinical laboratories.
• Development of multi-omics platforms combining proteomics, glycomics, lipidomics, and metabolomics in a unified workflow.

Conclusion

The joint 16th Central and Eastern European Proteomic Conference and 10th Czech Mass Spectrometry Conference underscored rapid technological progress and growing interdisciplinary applications of mass spectrometry and proteomics. Attendees gained deep insights into advanced instrumentation, innovative workflows, and translation into biomedicine, agriculture, and industry. Continued collaboration, standardization, and methodological innovation were identified as keys to realizing the full potential of multi-omics in precision medicine and global health.

Reference

Not applicable