Comparison of Measurements performed on Cary 8454 and Cary 60 UV-Vis Spectrophotometers

Significance of the Topic

Ensuring consistent analytical results when transferring methods between different UV-Vis spectrophotometers is essential for compliance with GMP guidelines and for maintaining data integrity across laboratories.

Objectives and Study Overview

The study aimed to verify that a UV-Vis method originally developed on an Agilent Cary 8454 system can be reliably transferred to a Cary 60 instrument. Two main tasks were undertaken: selection of an appropriate analysis wavelength and quantification of an unknown potassium dichromate sample.

Methodology

The workflow was divided into two parts

• Part 1: Peak determination using a 40 mg/L potassium dichromate solution. Cary 8454 required a one-hour lamp warm up and measurement in ChemStation Standard Mode. The Cary 60 was measured immediately using WinUV Scan mode. Both systems scanned from 200 to 800 nm to identify absorption maxima.
• Part 2: Concentration analysis at 350 nm. Calibration curves were constructed with standard solutions at 40, 80, and 120 mg/L. Measurements were performed with Quantification Task in ChemStation and Concentration application in WinUV software.

Instrumentation Used

• Cary 8454 UV-Vis spectrophotometer with ChemStation software
• Cary 60 UV-Vis spectrophotometer with WinUV software

Main Results and Discussion

Both instruments produced overlapping spectra with absorption peaks at 257 nm and 350 nm, confirming wavelength consistency. Calibration curves showed linear responses. The unknown sample concentration was found to be 77.5 mg/L on the Cary 8454 and 77.4 mg/L on the Cary 60, demonstrating excellent agreement and reproducibility.

Benefits and Practical Applications

• Rapid method development and transfer between instruments
• High reproducibility supporting routine QA/QC workflows
• Regulatory compliance through comparable performance

Future Trends and Potential Uses

Advances may include integration with 21 CFR Part 11 compliant software tools, automated data processing, enhanced miniaturization of UV-Vis platforms, and incorporation of data analytics for real-time monitoring.

Conclusion

The Cary 60 UV-Vis spectrophotometer effectively replicates the performance of the Cary 8454 for both spectral scanning and quantitative analysis, facilitating seamless method transfer and supporting regulated laboratory environments.