Metrohm i-Raman Plus Quick Start Guide

Importance of the Topic

Portable Raman spectroscopy has become an essential analytical technique for rapid, non-destructive identification of materials across fields such as pharmaceutical quality control, forensic analysis and field diagnostics. The ability to perform in situ measurements without extensive sample preparation and to detect molecular fingerprints makes handheld Raman systems invaluable in both laboratory and on-site applications.

Objectives and Overview of the Guide

This guide describes the setup, safety procedures and operational workflow for the i-Raman Plus portable Raman spectrometer. It aims to help users install hardware, connect safety interlocks, launch the BWSpec software and acquire reliable Raman spectra from various sample types, including liquids, powders and solid surfaces.

Methodology and Used Instrumentation

The core instrumentation and accessories include:

• i-Raman Plus portable Raman spectrometer with integrated laser and detector
• FC-type fiber probe with distance regulator and manual shutter
• Cuvette holder for liquid samples and BAC150 stage for solids and powders
• Safety interlock plug to disable laser emission when removed
• BWSpec software configured in Raman workspace for spectrum acquisition, dark subtraction and data export

Sample measurement approaches cover direct contact readings through plastic bags, pigment analysis on surfaces and cuvette-based liquid tests.

Main Procedures and Discussion

Hardware Installation and Safety Interlock

• Connect the fiber probe to the front panel laser emission port, aligning the FC key and red dots gently until locked
• Insert the safety interlock plug to enable laser power; removal immediately shuts off the laser
• Adjust the distance regulator or attach the BAC150 stage depending on sample form

Software Startup and Parameter Settings

• Install and launch BWSpec, selecting the Raman workspace and confirming an online status
• Set integration time (typically several seconds) and multiplier to position the highest peak near 50 000 counts
• Perform a dark scan, then acquire sample spectra using single, overlay or continuous modes
• Monitor laser power percentage and scale settings for optimal signal-to-noise ratio
• Save spectra in preferred formats such as TXT or SPC

Operational Precautions

• Wear appropriate laser safety goggles and eliminate ambient or fluorescent light sources
• Reduce laser power for dark or heat-sensitive samples such as plastics and explosives
• Ensure probe shutter is open and software remains connected before each acquisition

Benefits and Practical Applications

The i-Raman Plus system offers:

• High portability for field and in-line monitoring
• Minimal sample preparation across liquid, powder and solid matrices
• Rapid spectral acquisition with dark subtraction for improved baseline correction
• Versatility in detecting a broad range of materials, from active pharmaceutical ingredients to inorganic compounds

This flexibility supports applications in pharmaceutical QA/QC, material verification, forensic screening and cultural heritage analysis.

Future Trends and Potential Applications

Advancements in portable Raman technology are expected to focus on:

• Integration with machine learning algorithms for automated spectral interpretation
• Enhanced connectivity for real-time cloud-based data sharing
• Miniaturization and battery optimization to extend field deployment
• Expanded spectral libraries for more comprehensive material identification

In addition, coupling Raman with complementary techniques such as fluorescence or infrared spectroscopy may broaden analytical capabilities.

Conclusion

The i-Raman Plus Quick Start Guide provides a clear, step-by-step framework for safe installation and operation of a portable Raman spectrometer. By following the outlined hardware connections, software configurations and measurement protocols, users can obtain high-quality Raman spectra efficiently. This enables reliable material identification and paves the way for diverse applications in research, industry and field analysis.

References

B&W Tek, LLC. i-Raman Plus Quick Start Guide, document number 290020251-C, September 2018.